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pgex 4t 2 expression vector  (Danaher Inc)


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    Structured Review

    Danaher Inc pgex 4t 2 expression vector
    Pgex 4t 2 Expression Vector, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 95/100, based on 918 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lambda-zap+express+vector/PGEX-4T-2/pmc03516578-90-59-63
    Average 95 stars, based on 918 article reviews
    pgex 4t 2 expression vector - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Transformation Assay:

    Article Title: Maize CDKA2;1a and CDKB1;1 kinases have different requirements for their activation and participate in substrate recognition.
    Article Snippet: His-CDKA2;1a, His-CDKB1;1, MBP-CycD2;2a, MBPCycB2;1 proteins and His-CDKA2;1a/CycD2;2a-MBP, HisCDKB1;1/CycD2;2a-MBP complexes that included the wild-type or the mutant versions T161A, Y15A, K60A, T197A and T197 were induced from the Tuner (DE3) E. coli strain (Novagene), previously transformed with the plasmids described above (Table 2). .. GST-RBRCT protein was induced from the Rosetta2 (DE3) E. coli strain (Novagene), and transformed with the pDEST15*ZmRBRct plasmid [89], while GST-CKS protein was induced from the Tuner (DE3) E. coli strain and transformed with the pGEX4T-2*ZmCKS plasmid (not-published work); GST and MBP proteins were produced after overexpression of E. coli cells (Tuner (DE3) strain) transformed with pGEX4T-2 (GE Healthcare, Chicago, IL, USA) y pMAL-c5x (NEB) plasmids. ..

    Article Title: OsDREB2A , a Rice Transcription Factor, Significantly Affects Salt Tolerance in Transgenic Soybean
    Article Snippet: .. This fragment was cloned into the Bam H I/ Xho I sites of the pGEX-4T-2 vector (Amersham Biosciences, Piscataway, USA) and transformed into Escherichia coli BL21 cells (Amersham Biosciences, Piscataway, USA) to produce the GST-fusion protein. .. The GST-fusion protein was purified using a glutathione Sepharose 4B column according to the manufacturer’s instruction (Amersham Biosciences, Piscataway, USA).

    Plasmid Preparation:

    Article Title: Maize CDKA2;1a and CDKB1;1 kinases have different requirements for their activation and participate in substrate recognition.
    Article Snippet: His-CDKA2;1a, His-CDKB1;1, MBP-CycD2;2a, MBPCycB2;1 proteins and His-CDKA2;1a/CycD2;2a-MBP, HisCDKB1;1/CycD2;2a-MBP complexes that included the wild-type or the mutant versions T161A, Y15A, K60A, T197A and T197 were induced from the Tuner (DE3) E. coli strain (Novagene), previously transformed with the plasmids described above (Table 2). .. GST-RBRCT protein was induced from the Rosetta2 (DE3) E. coli strain (Novagene), and transformed with the pDEST15*ZmRBRct plasmid [89], while GST-CKS protein was induced from the Tuner (DE3) E. coli strain and transformed with the pGEX4T-2*ZmCKS plasmid (not-published work); GST and MBP proteins were produced after overexpression of E. coli cells (Tuner (DE3) strain) transformed with pGEX4T-2 (GE Healthcare, Chicago, IL, USA) y pMAL-c5x (NEB) plasmids. ..

    Article Title: OsDREB2A , a Rice Transcription Factor, Significantly Affects Salt Tolerance in Transgenic Soybean
    Article Snippet: .. This fragment was cloned into the Bam H I/ Xho I sites of the pGEX-4T-2 vector (Amersham Biosciences, Piscataway, USA) and transformed into Escherichia coli BL21 cells (Amersham Biosciences, Piscataway, USA) to produce the GST-fusion protein. .. The GST-fusion protein was purified using a glutathione Sepharose 4B column according to the manufacturer’s instruction (Amersham Biosciences, Piscataway, USA).

    Article Title: Big Domains Are Novel Ca 2+ -Binding Modules: Evidences from Big Domains of Leptospira Immunoglobulin-Like (Lig) Proteins
    Article Snippet: .. Briefly, the PCR products of LigCon (first 599 residues of common region of LigA and B), Lig A3 (residues 281–378 of common region) and Lig A4 (common region residues 379–466) were cloned into SmaI and XhoI restriction sites of pGex4T-2 vector (Amersham Biosciences) as GST fusion. ..

    Article Title: Sterol carrier protein-x gene and effects of sterol carrier protein-2 inhibitors on lipid uptake in Manduca sexta
    Article Snippet: .. To produce recombinant MsSCP-2 (rMsSCP-2), PCR products of the entire coding region of the MsSCP-2 gene were cloned into the pGEX-4T-2 GST tag vector (Amersham Pharmacia). ..

    Produced:

    Article Title: Maize CDKA2;1a and CDKB1;1 kinases have different requirements for their activation and participate in substrate recognition.
    Article Snippet: His-CDKA2;1a, His-CDKB1;1, MBP-CycD2;2a, MBPCycB2;1 proteins and His-CDKA2;1a/CycD2;2a-MBP, HisCDKB1;1/CycD2;2a-MBP complexes that included the wild-type or the mutant versions T161A, Y15A, K60A, T197A and T197 were induced from the Tuner (DE3) E. coli strain (Novagene), previously transformed with the plasmids described above (Table 2). .. GST-RBRCT protein was induced from the Rosetta2 (DE3) E. coli strain (Novagene), and transformed with the pDEST15*ZmRBRct plasmid [89], while GST-CKS protein was induced from the Tuner (DE3) E. coli strain and transformed with the pGEX4T-2*ZmCKS plasmid (not-published work); GST and MBP proteins were produced after overexpression of E. coli cells (Tuner (DE3) strain) transformed with pGEX4T-2 (GE Healthcare, Chicago, IL, USA) y pMAL-c5x (NEB) plasmids. ..

    Over Expression:

    Article Title: Maize CDKA2;1a and CDKB1;1 kinases have different requirements for their activation and participate in substrate recognition.
    Article Snippet: His-CDKA2;1a, His-CDKB1;1, MBP-CycD2;2a, MBPCycB2;1 proteins and His-CDKA2;1a/CycD2;2a-MBP, HisCDKB1;1/CycD2;2a-MBP complexes that included the wild-type or the mutant versions T161A, Y15A, K60A, T197A and T197 were induced from the Tuner (DE3) E. coli strain (Novagene), previously transformed with the plasmids described above (Table 2). .. GST-RBRCT protein was induced from the Rosetta2 (DE3) E. coli strain (Novagene), and transformed with the pDEST15*ZmRBRct plasmid [89], while GST-CKS protein was induced from the Tuner (DE3) E. coli strain and transformed with the pGEX4T-2*ZmCKS plasmid (not-published work); GST and MBP proteins were produced after overexpression of E. coli cells (Tuner (DE3) strain) transformed with pGEX4T-2 (GE Healthcare, Chicago, IL, USA) y pMAL-c5x (NEB) plasmids. ..

    Clone Assay:

    Article Title: OsDREB2A , a Rice Transcription Factor, Significantly Affects Salt Tolerance in Transgenic Soybean
    Article Snippet: .. This fragment was cloned into the Bam H I/ Xho I sites of the pGEX-4T-2 vector (Amersham Biosciences, Piscataway, USA) and transformed into Escherichia coli BL21 cells (Amersham Biosciences, Piscataway, USA) to produce the GST-fusion protein. .. The GST-fusion protein was purified using a glutathione Sepharose 4B column according to the manufacturer’s instruction (Amersham Biosciences, Piscataway, USA).

    Article Title: Big Domains Are Novel Ca 2+ -Binding Modules: Evidences from Big Domains of Leptospira Immunoglobulin-Like (Lig) Proteins
    Article Snippet: .. Briefly, the PCR products of LigCon (first 599 residues of common region of LigA and B), Lig A3 (residues 281–378 of common region) and Lig A4 (common region residues 379–466) were cloned into SmaI and XhoI restriction sites of pGex4T-2 vector (Amersham Biosciences) as GST fusion. ..

    Article Title: Sterol carrier protein-x gene and effects of sterol carrier protein-2 inhibitors on lipid uptake in Manduca sexta
    Article Snippet: .. To produce recombinant MsSCP-2 (rMsSCP-2), PCR products of the entire coding region of the MsSCP-2 gene were cloned into the pGEX-4T-2 GST tag vector (Amersham Pharmacia). ..

    Polymerase Chain Reaction:

    Article Title: Big Domains Are Novel Ca 2+ -Binding Modules: Evidences from Big Domains of Leptospira Immunoglobulin-Like (Lig) Proteins
    Article Snippet: .. Briefly, the PCR products of LigCon (first 599 residues of common region of LigA and B), Lig A3 (residues 281–378 of common region) and Lig A4 (common region residues 379–466) were cloned into SmaI and XhoI restriction sites of pGex4T-2 vector (Amersham Biosciences) as GST fusion. ..

    Article Title: Sterol carrier protein-x gene and effects of sterol carrier protein-2 inhibitors on lipid uptake in Manduca sexta
    Article Snippet: .. To produce recombinant MsSCP-2 (rMsSCP-2), PCR products of the entire coding region of the MsSCP-2 gene were cloned into the pGEX-4T-2 GST tag vector (Amersham Pharmacia). ..

    Recombinant:

    Article Title: Sterol carrier protein-x gene and effects of sterol carrier protein-2 inhibitors on lipid uptake in Manduca sexta
    Article Snippet: .. To produce recombinant MsSCP-2 (rMsSCP-2), PCR products of the entire coding region of the MsSCP-2 gene were cloned into the pGEX-4T-2 GST tag vector (Amersham Pharmacia). ..

    Generated:

    Article Title: H-NOX Regulates Biofilm Formation in Agrobacterium Vitis in Response to NO
    Article Snippet: After washing, the protein was eluted with 250 mM imidazole, and the eluted protein was desalted using a PD-10 column (GE Healthcare Life Sciences) and stored at −80 ° C. Expression and purification of GST-tagged WT av HaCE. .. WT HaCE ( avi_3097) containing GST at the N-terminus, was generated by QuikChange site-directed mutagenesis using a GST-tagged av GGAAF construct in vector pGEX-4T-2 (GE Healthcare) as the template. ..

    Mutagenesis:

    Article Title: H-NOX Regulates Biofilm Formation in Agrobacterium Vitis in Response to NO
    Article Snippet: After washing, the protein was eluted with 250 mM imidazole, and the eluted protein was desalted using a PD-10 column (GE Healthcare Life Sciences) and stored at −80 ° C. Expression and purification of GST-tagged WT av HaCE. .. WT HaCE ( avi_3097) containing GST at the N-terminus, was generated by QuikChange site-directed mutagenesis using a GST-tagged av GGAAF construct in vector pGEX-4T-2 (GE Healthcare) as the template. ..

    Construct:

    Article Title: H-NOX Regulates Biofilm Formation in Agrobacterium Vitis in Response to NO
    Article Snippet: After washing, the protein was eluted with 250 mM imidazole, and the eluted protein was desalted using a PD-10 column (GE Healthcare Life Sciences) and stored at −80 ° C. Expression and purification of GST-tagged WT av HaCE. .. WT HaCE ( avi_3097) containing GST at the N-terminus, was generated by QuikChange site-directed mutagenesis using a GST-tagged av GGAAF construct in vector pGEX-4T-2 (GE Healthcare) as the template. ..



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    Image Search Results


    ( A ) The expression vector was subsequently inserted into the λ phage, generating the recombinant NBPs, ( B ) Different cell types were infected with the indicated recombinant λ NBPs at an MOI of 10 PFU/cell and apoptin transcription was analyzed by RT-PCR, ( C ) RT-PCR result for λ ZAP-CMV vector treated cells that have no expression of apoptin, ( D ) Western blot analysis to detect apoptin protein from cells supernatants and lysates. To analyze apoptin expression, the cells were infected with the indicated recombinant NBPs. Purified apoptin was used as a positive control and CAV infected BT-474 cell was used as a negative control.

    Journal: PLoS ONE

    Article Title: λ Phage Nanobioparticle Expressing Apoptin Efficiently Suppress Human Breast Carcinoma Tumor Growth In Vivo

    doi: 10.1371/journal.pone.0079907

    Figure Lengend Snippet: ( A ) The expression vector was subsequently inserted into the λ phage, generating the recombinant NBPs, ( B ) Different cell types were infected with the indicated recombinant λ NBPs at an MOI of 10 PFU/cell and apoptin transcription was analyzed by RT-PCR, ( C ) RT-PCR result for λ ZAP-CMV vector treated cells that have no expression of apoptin, ( D ) Western blot analysis to detect apoptin protein from cells supernatants and lysates. To analyze apoptin expression, the cells were infected with the indicated recombinant NBPs. Purified apoptin was used as a positive control and CAV infected BT-474 cell was used as a negative control.

    Article Snippet: Large scale NBPs containing λ ZAP-CMV-apoptin expressing vector was conducted in 1 l of M9 medium (Sigma-Aldrich, USA) containing ampicillin (Sigma, USA).

    Techniques: Expressing, Plasmid Preparation, Recombinant, Infection, Reverse Transcription Polymerase Chain Reaction, Western Blot, Purification, Positive Control, Negative Control

    Immunostaining of apoptin protein showed that in can express in breast carcinoma cell lines after 12λ ZAP-CMV vector have not any apoptosis after 36 h as same as untreated cells.

    Journal: PLoS ONE

    Article Title: λ Phage Nanobioparticle Expressing Apoptin Efficiently Suppress Human Breast Carcinoma Tumor Growth In Vivo

    doi: 10.1371/journal.pone.0079907

    Figure Lengend Snippet: Immunostaining of apoptin protein showed that in can express in breast carcinoma cell lines after 12λ ZAP-CMV vector have not any apoptosis after 36 h as same as untreated cells.

    Article Snippet: Large scale NBPs containing λ ZAP-CMV-apoptin expressing vector was conducted in 1 l of M9 medium (Sigma-Aldrich, USA) containing ampicillin (Sigma, USA).

    Techniques: Immunostaining, Plasmid Preparation

    BT-474, SKBR-3 and ZR-75 cells were examined after 36 h of transfection by flowcytometry. All the cell lines were susceptible to NBPs apoptin-induced apoptosis. We have not apoptosis in vector treated group and untreated group.

    Journal: PLoS ONE

    Article Title: λ Phage Nanobioparticle Expressing Apoptin Efficiently Suppress Human Breast Carcinoma Tumor Growth In Vivo

    doi: 10.1371/journal.pone.0079907

    Figure Lengend Snippet: BT-474, SKBR-3 and ZR-75 cells were examined after 36 h of transfection by flowcytometry. All the cell lines were susceptible to NBPs apoptin-induced apoptosis. We have not apoptosis in vector treated group and untreated group.

    Article Snippet: Large scale NBPs containing λ ZAP-CMV-apoptin expressing vector was conducted in 1 l of M9 medium (Sigma-Aldrich, USA) containing ampicillin (Sigma, USA).

    Techniques: Transfection, Plasmid Preparation

    BT-474 breast carcinoma cell line transfected with λ ZAP-CMV-apoptin, λ ZAP-CMV vector and λ phage (vehicle) construct stained with FITC immunostaining and then visualized by fluorescence microscopy. There was no sign of cell necrosis after the treatments. There is only apoptotic morphology of cells after treatment with λ ZAP-CMV-apoptin.

    Journal: PLoS ONE

    Article Title: λ Phage Nanobioparticle Expressing Apoptin Efficiently Suppress Human Breast Carcinoma Tumor Growth In Vivo

    doi: 10.1371/journal.pone.0079907

    Figure Lengend Snippet: BT-474 breast carcinoma cell line transfected with λ ZAP-CMV-apoptin, λ ZAP-CMV vector and λ phage (vehicle) construct stained with FITC immunostaining and then visualized by fluorescence microscopy. There was no sign of cell necrosis after the treatments. There is only apoptotic morphology of cells after treatment with λ ZAP-CMV-apoptin.

    Article Snippet: Large scale NBPs containing λ ZAP-CMV-apoptin expressing vector was conducted in 1 l of M9 medium (Sigma-Aldrich, USA) containing ampicillin (Sigma, USA).

    Techniques: Transfection, Plasmid Preparation, Construct, Staining, Immunostaining, Fluorescence, Microscopy

    ( A ) Histochemistry analysis of tumor tissue sections showing apoptotic changes. The untreated tumor tissue contains many dividing cells. After 96 h treatment with NBPs there are a few cells maintained in the tumor tissue that could proliferate. Tumor growth was markedly suppressed in the apoptin treated group, ( B ) Histological examination of other organs (brain and heart) in tumor bearing mice that is not involved in the pathological changes of BT-474 cells. There are no changes in morphology of the brain and/or heart tissues and they are as same as control groups.

    Journal: PLoS ONE

    Article Title: λ Phage Nanobioparticle Expressing Apoptin Efficiently Suppress Human Breast Carcinoma Tumor Growth In Vivo

    doi: 10.1371/journal.pone.0079907

    Figure Lengend Snippet: ( A ) Histochemistry analysis of tumor tissue sections showing apoptotic changes. The untreated tumor tissue contains many dividing cells. After 96 h treatment with NBPs there are a few cells maintained in the tumor tissue that could proliferate. Tumor growth was markedly suppressed in the apoptin treated group, ( B ) Histological examination of other organs (brain and heart) in tumor bearing mice that is not involved in the pathological changes of BT-474 cells. There are no changes in morphology of the brain and/or heart tissues and they are as same as control groups.

    Article Snippet: Large scale NBPs containing λ ZAP-CMV-apoptin expressing vector was conducted in 1 l of M9 medium (Sigma-Aldrich, USA) containing ampicillin (Sigma, USA).

    Techniques:

    ( A ) TUNEL analysis revealed that apoptin induces the apoptotic activity in neoplasms (Magnification: ×400), ( B ) Analysis of survival. Mice treated with NBPs survived longer than the mice in the other 2 groups and the mean survival of NBPs-infected mice were >90 days (p<0.005). Fifty days after the beginning of the treatment, 90% of the animals infected by NBPs were alive, while at this time 100% of vector treated mice and 100% of saline-treated mice had died. Tumor-bearing mice treated with saline had a mean survival of 50 days.

    Journal: PLoS ONE

    Article Title: λ Phage Nanobioparticle Expressing Apoptin Efficiently Suppress Human Breast Carcinoma Tumor Growth In Vivo

    doi: 10.1371/journal.pone.0079907

    Figure Lengend Snippet: ( A ) TUNEL analysis revealed that apoptin induces the apoptotic activity in neoplasms (Magnification: ×400), ( B ) Analysis of survival. Mice treated with NBPs survived longer than the mice in the other 2 groups and the mean survival of NBPs-infected mice were >90 days (p<0.005). Fifty days after the beginning of the treatment, 90% of the animals infected by NBPs were alive, while at this time 100% of vector treated mice and 100% of saline-treated mice had died. Tumor-bearing mice treated with saline had a mean survival of 50 days.

    Article Snippet: Large scale NBPs containing λ ZAP-CMV-apoptin expressing vector was conducted in 1 l of M9 medium (Sigma-Aldrich, USA) containing ampicillin (Sigma, USA).

    Techniques: TUNEL Assay, Activity Assay, Infection, Plasmid Preparation