pgex 4t 2 expression vector (Danaher Inc)
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Pgex 4t 2 Expression Vector, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 95/100, based on 918 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lambda-zap+express+vector/PGEX-4T-2/pmc03516578-90-59-63
Average 95 stars, based on 918 article reviews
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Transformation Assay:Article Title: Maize CDKA2;1a and CDKB1;1 kinases have different requirements for their activation and participate in substrate recognition. Article Snippet: His-CDKA2;1a, His-CDKB1;1, MBP-CycD2;2a, MBPCycB2;1 proteins and His-CDKA2;1a/CycD2;2a-MBP, HisCDKB1;1/CycD2;2a-MBP complexes that included the wild-type or the mutant versions T161A, Y15A, K60A, T197A and T197 were induced from the Tuner (DE3) E. coli strain (Novagene), previously transformed with the plasmids described above (Table 2). .. GST-RBRCT protein was induced from the Rosetta2 (DE3) E. coli strain (Novagene), and transformed with the pDEST15*ZmRBRct plasmid [89], while GST-CKS protein was induced from the Tuner (DE3) E. coli strain and transformed with the Article Title: OsDREB2A , a Rice Transcription Factor, Significantly Affects Salt Tolerance in Transgenic Soybean Article Snippet: .. This fragment was cloned into the Bam H I/ Xho I sites of the Plasmid Preparation:Article Title: Maize CDKA2;1a and CDKB1;1 kinases have different requirements for their activation and participate in substrate recognition. Article Snippet: His-CDKA2;1a, His-CDKB1;1, MBP-CycD2;2a, MBPCycB2;1 proteins and His-CDKA2;1a/CycD2;2a-MBP, HisCDKB1;1/CycD2;2a-MBP complexes that included the wild-type or the mutant versions T161A, Y15A, K60A, T197A and T197 were induced from the Tuner (DE3) E. coli strain (Novagene), previously transformed with the plasmids described above (Table 2). .. GST-RBRCT protein was induced from the Rosetta2 (DE3) E. coli strain (Novagene), and transformed with the pDEST15*ZmRBRct plasmid [89], while GST-CKS protein was induced from the Tuner (DE3) E. coli strain and transformed with the Article Title: OsDREB2A , a Rice Transcription Factor, Significantly Affects Salt Tolerance in Transgenic Soybean Article Snippet: .. This fragment was cloned into the Bam H I/ Xho I sites of the Article Title: Big Domains Are Novel Ca 2+ -Binding Modules: Evidences from Big Domains of Leptospira Immunoglobulin-Like (Lig) Proteins Article Snippet: .. Briefly, the PCR products of LigCon (first 599 residues of common region of LigA and B), Lig A3 (residues 281–378 of common region) and Lig A4 (common region residues 379–466) were cloned into SmaI and XhoI restriction sites of Article Title: Sterol carrier protein-x gene and effects of sterol carrier protein-2 inhibitors on lipid uptake in Manduca sexta Article Snippet: .. To produce recombinant MsSCP-2 (rMsSCP-2), PCR products of the entire coding region of the MsSCP-2 gene were cloned into the Produced:Article Title: Maize CDKA2;1a and CDKB1;1 kinases have different requirements for their activation and participate in substrate recognition. Article Snippet: His-CDKA2;1a, His-CDKB1;1, MBP-CycD2;2a, MBPCycB2;1 proteins and His-CDKA2;1a/CycD2;2a-MBP, HisCDKB1;1/CycD2;2a-MBP complexes that included the wild-type or the mutant versions T161A, Y15A, K60A, T197A and T197 were induced from the Tuner (DE3) E. coli strain (Novagene), previously transformed with the plasmids described above (Table 2). .. GST-RBRCT protein was induced from the Rosetta2 (DE3) E. coli strain (Novagene), and transformed with the pDEST15*ZmRBRct plasmid [89], while GST-CKS protein was induced from the Tuner (DE3) E. coli strain and transformed with the Over Expression:Article Title: Maize CDKA2;1a and CDKB1;1 kinases have different requirements for their activation and participate in substrate recognition. Article Snippet: His-CDKA2;1a, His-CDKB1;1, MBP-CycD2;2a, MBPCycB2;1 proteins and His-CDKA2;1a/CycD2;2a-MBP, HisCDKB1;1/CycD2;2a-MBP complexes that included the wild-type or the mutant versions T161A, Y15A, K60A, T197A and T197 were induced from the Tuner (DE3) E. coli strain (Novagene), previously transformed with the plasmids described above (Table 2). .. GST-RBRCT protein was induced from the Rosetta2 (DE3) E. coli strain (Novagene), and transformed with the pDEST15*ZmRBRct plasmid [89], while GST-CKS protein was induced from the Tuner (DE3) E. coli strain and transformed with the Clone Assay:Article Title: OsDREB2A , a Rice Transcription Factor, Significantly Affects Salt Tolerance in Transgenic Soybean Article Snippet: .. This fragment was cloned into the Bam H I/ Xho I sites of the Article Title: Big Domains Are Novel Ca 2+ -Binding Modules: Evidences from Big Domains of Leptospira Immunoglobulin-Like (Lig) Proteins Article Snippet: .. Briefly, the PCR products of LigCon (first 599 residues of common region of LigA and B), Lig A3 (residues 281–378 of common region) and Lig A4 (common region residues 379–466) were cloned into SmaI and XhoI restriction sites of Article Title: Sterol carrier protein-x gene and effects of sterol carrier protein-2 inhibitors on lipid uptake in Manduca sexta Article Snippet: .. To produce recombinant MsSCP-2 (rMsSCP-2), PCR products of the entire coding region of the MsSCP-2 gene were cloned into the Polymerase Chain Reaction:Article Title: Big Domains Are Novel Ca 2+ -Binding Modules: Evidences from Big Domains of Leptospira Immunoglobulin-Like (Lig) Proteins Article Snippet: .. Briefly, the PCR products of LigCon (first 599 residues of common region of LigA and B), Lig A3 (residues 281–378 of common region) and Lig A4 (common region residues 379–466) were cloned into SmaI and XhoI restriction sites of Article Title: Sterol carrier protein-x gene and effects of sterol carrier protein-2 inhibitors on lipid uptake in Manduca sexta Article Snippet: .. To produce recombinant MsSCP-2 (rMsSCP-2), PCR products of the entire coding region of the MsSCP-2 gene were cloned into the Recombinant:Article Title: Sterol carrier protein-x gene and effects of sterol carrier protein-2 inhibitors on lipid uptake in Manduca sexta Article Snippet: .. To produce recombinant MsSCP-2 (rMsSCP-2), PCR products of the entire coding region of the MsSCP-2 gene were cloned into the Generated:Article Title: H-NOX Regulates Biofilm Formation in Agrobacterium Vitis in Response to NO Article Snippet: After washing, the protein was eluted with 250 mM imidazole, and the eluted protein was desalted using a PD-10 column (GE Healthcare Life Sciences) and stored at −80 ° C. Expression and purification of GST-tagged WT av HaCE. .. WT HaCE ( avi_3097) containing GST at the N-terminus, was generated by QuikChange site-directed mutagenesis using a GST-tagged av GGAAF construct in Mutagenesis:Article Title: H-NOX Regulates Biofilm Formation in Agrobacterium Vitis in Response to NO Article Snippet: After washing, the protein was eluted with 250 mM imidazole, and the eluted protein was desalted using a PD-10 column (GE Healthcare Life Sciences) and stored at −80 ° C. Expression and purification of GST-tagged WT av HaCE. .. WT HaCE ( avi_3097) containing GST at the N-terminus, was generated by QuikChange site-directed mutagenesis using a GST-tagged av GGAAF construct in Construct:Article Title: H-NOX Regulates Biofilm Formation in Agrobacterium Vitis in Response to NO Article Snippet: After washing, the protein was eluted with 250 mM imidazole, and the eluted protein was desalted using a PD-10 column (GE Healthcare Life Sciences) and stored at −80 ° C. Expression and purification of GST-tagged WT av HaCE. .. WT HaCE ( avi_3097) containing GST at the N-terminus, was generated by QuikChange site-directed mutagenesis using a GST-tagged av GGAAF construct in |
