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Promega mboi
Mboi, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lambda-zap+express+vector/mboi/pm09723925-152-6-7
Average 90 stars, based on 1 article reviews
mboi - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Terminal Restriction Fragment Length Polymorphism:

Article Title: Microform-related community patterns of methane-cycling microbes in boreal Sphagnum bogs are site specific.
Article Snippet: Each 50-μl PCR reaction contained 0.5 μM of each primer, 200 μM of dNTPs and 1 U of DNA polymerase (Biotools B & M Labs S.A., Madrid, Spain) in 1 × reaction buffer and 1 μl of template DNA. .. Thermal cycling was started by initial denaturation at 94◦C for 3 min, followed by 40 cycles of denaturation at 94◦C for 30 s, annealing at 55◦C for 45 s, extension at 72◦C for 30 s and a final extension at 72◦C for 5 min. Products were digested for T-RFLP analysis with MboI (3 U, Promega) and HhaI (3 U, Promega) in a volume of 20 μl for 6 h. The enzymes were selected for optimal separation of methanogens common in northern peatlands based on previous sequence data from 11 peatlands (Martı́ et al. 2015). .. Digested products were ethanol precipitated, dissolved in 10 μl of Hi-Di formamide (Applied Biosystems) and 4 μl of the sample was mixed with 0.2 μl of 600-bp standard (Beckmann Coulter) and Sample Loading Solution (Beckmann Coulter) to a total volume of 30 μl.

Northern Blot:

Article Title: Microform-related community patterns of methane-cycling microbes in boreal Sphagnum bogs are site specific.
Article Snippet: Each 50-μl PCR reaction contained 0.5 μM of each primer, 200 μM of dNTPs and 1 U of DNA polymerase (Biotools B & M Labs S.A., Madrid, Spain) in 1 × reaction buffer and 1 μl of template DNA. .. Thermal cycling was started by initial denaturation at 94◦C for 3 min, followed by 40 cycles of denaturation at 94◦C for 30 s, annealing at 55◦C for 45 s, extension at 72◦C for 30 s and a final extension at 72◦C for 5 min. Products were digested for T-RFLP analysis with MboI (3 U, Promega) and HhaI (3 U, Promega) in a volume of 20 μl for 6 h. The enzymes were selected for optimal separation of methanogens common in northern peatlands based on previous sequence data from 11 peatlands (Martı́ et al. 2015). .. Digested products were ethanol precipitated, dissolved in 10 μl of Hi-Di formamide (Applied Biosystems) and 4 μl of the sample was mixed with 0.2 μl of 600-bp standard (Beckmann Coulter) and Sample Loading Solution (Beckmann Coulter) to a total volume of 30 μl.

Sequencing:

Article Title: Microform-related community patterns of methane-cycling microbes in boreal Sphagnum bogs are site specific.
Article Snippet: Each 50-μl PCR reaction contained 0.5 μM of each primer, 200 μM of dNTPs and 1 U of DNA polymerase (Biotools B & M Labs S.A., Madrid, Spain) in 1 × reaction buffer and 1 μl of template DNA. .. Thermal cycling was started by initial denaturation at 94◦C for 3 min, followed by 40 cycles of denaturation at 94◦C for 30 s, annealing at 55◦C for 45 s, extension at 72◦C for 30 s and a final extension at 72◦C for 5 min. Products were digested for T-RFLP analysis with MboI (3 U, Promega) and HhaI (3 U, Promega) in a volume of 20 μl for 6 h. The enzymes were selected for optimal separation of methanogens common in northern peatlands based on previous sequence data from 11 peatlands (Martı́ et al. 2015). .. Digested products were ethanol precipitated, dissolved in 10 μl of Hi-Di formamide (Applied Biosystems) and 4 μl of the sample was mixed with 0.2 μl of 600-bp standard (Beckmann Coulter) and Sample Loading Solution (Beckmann Coulter) to a total volume of 30 μl.

Polymerase Chain Reaction:

Article Title: Herbivory and Soil Water Availability Induce Changes in Arbuscular Mycorrhizal Fungal Abundance and Composition.
Article Snippet: We tested the prediction that abundance and composition of arbuscular mycorrhizal fungi (AMF) in Ipomopsis aggregata roots and soils are influenced by ungulate herbivory and drought conditions by examining the effects in a field setting over two years.. We used a multi-metric approach to quantify AMF root colonization, AMF reproduction, and AMF community composition in roots and soils.. We incorporated complimentary community characterization assays by morphologically identifying spores from trap cultures and the use of terminal restriction fragment length polymorphism (T-RFLP) fingerprinting.

Article Title: A Founder Mutation in PET100 Causes Isolated Complex IV Deficiency in Lebanese Individuals with Leigh Syndrome
Article Snippet: .. RFLP Analysis PCR product amplified by forward primer 5′-gccagacccgttttctattg-3′ and reverse primer 5′-aacccactcctgtgtccact-3′ was digested for 5 hr with MboI (Promega) as per manufacturer’s protocol and resolved on 2% agarose gels. .. Sequenom Genotyping Assay A specifically designed multiplexed MALDI-TOF mass spectrometry (Sequenom) assay was used to genotype 81 unrelated Lebanese individuals for the c.3G>C PET100 mutation.

Article Title: Long-term legacies and partial recovery of mycorrhizal communities after invasive plant removal
Article Snippet: Invasive plants can have strong impacts on native communities, which have prompted intense efforts at invasive removal.. However, relatively little is known about how native communities will reassemble after a dominant invader has been removed from the system.. Legacy effects of invasive plants on soil microbial communities may alter native plant community reassembly long after the invader is gone.

Electrophoresis:

Article Title: Herbivory and Soil Water Availability Induce Changes in Arbuscular Mycorrhizal Fungal Abundance and Composition.
Article Snippet: We tested the prediction that abundance and composition of arbuscular mycorrhizal fungi (AMF) in Ipomopsis aggregata roots and soils are influenced by ungulate herbivory and drought conditions by examining the effects in a field setting over two years.. We used a multi-metric approach to quantify AMF root colonization, AMF reproduction, and AMF community composition in roots and soils.. We incorporated complimentary community characterization assays by morphologically identifying spores from trap cultures and the use of terminal restriction fragment length polymorphism (T-RFLP) fingerprinting.

Article Title: Long-term legacies and partial recovery of mycorrhizal communities after invasive plant removal
Article Snippet: Invasive plants can have strong impacts on native communities, which have prompted intense efforts at invasive removal.. However, relatively little is known about how native communities will reassemble after a dominant invader has been removed from the system.. Legacy effects of invasive plants on soil microbial communities may alter native plant community reassembly long after the invader is gone.

Amplification:

Article Title: A Founder Mutation in PET100 Causes Isolated Complex IV Deficiency in Lebanese Individuals with Leigh Syndrome
Article Snippet: .. RFLP Analysis PCR product amplified by forward primer 5′-gccagacccgttttctattg-3′ and reverse primer 5′-aacccactcctgtgtccact-3′ was digested for 5 hr with MboI (Promega) as per manufacturer’s protocol and resolved on 2% agarose gels. .. Sequenom Genotyping Assay A specifically designed multiplexed MALDI-TOF mass spectrometry (Sequenom) assay was used to genotype 81 unrelated Lebanese individuals for the c.3G>C PET100 mutation.

other:

Article Title: Fungi in the future: interannual variation and effects of atmospheric change on arbuscular mycorrhizal fungal communities
Article Snippet: A further digestion using AluI or MboI (Promega), or additional sequencing, was performed if sequence analysis revealed that further differentiation of the initial RFLP types was required to confidently assign molecular operational taxonomic units (MOTUs).



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( A ) The expression vector was subsequently inserted into the λ phage, generating the recombinant NBPs, ( B ) Different cell types were infected with the indicated recombinant λ NBPs at an MOI of 10 PFU/cell and apoptin transcription was analyzed by RT-PCR, ( C ) RT-PCR result for λ ZAP-CMV vector treated cells that have no expression of apoptin, ( D ) Western blot analysis to detect apoptin protein from cells supernatants and lysates. To analyze apoptin expression, the cells were infected with the indicated recombinant NBPs. Purified apoptin was used as a positive control and CAV infected BT-474 cell was used as a negative control.

Journal: PLoS ONE

Article Title: λ Phage Nanobioparticle Expressing Apoptin Efficiently Suppress Human Breast Carcinoma Tumor Growth In Vivo

doi: 10.1371/journal.pone.0079907

Figure Lengend Snippet: ( A ) The expression vector was subsequently inserted into the λ phage, generating the recombinant NBPs, ( B ) Different cell types were infected with the indicated recombinant λ NBPs at an MOI of 10 PFU/cell and apoptin transcription was analyzed by RT-PCR, ( C ) RT-PCR result for λ ZAP-CMV vector treated cells that have no expression of apoptin, ( D ) Western blot analysis to detect apoptin protein from cells supernatants and lysates. To analyze apoptin expression, the cells were infected with the indicated recombinant NBPs. Purified apoptin was used as a positive control and CAV infected BT-474 cell was used as a negative control.

Article Snippet: Large scale NBPs containing λ ZAP-CMV-apoptin expressing vector was conducted in 1 l of M9 medium (Sigma-Aldrich, USA) containing ampicillin (Sigma, USA).

Techniques: Expressing, Plasmid Preparation, Recombinant, Infection, Reverse Transcription Polymerase Chain Reaction, Western Blot, Purification, Positive Control, Negative Control

Immunostaining of apoptin protein showed that in can express in breast carcinoma cell lines after 12λ ZAP-CMV vector have not any apoptosis after 36 h as same as untreated cells.

Journal: PLoS ONE

Article Title: λ Phage Nanobioparticle Expressing Apoptin Efficiently Suppress Human Breast Carcinoma Tumor Growth In Vivo

doi: 10.1371/journal.pone.0079907

Figure Lengend Snippet: Immunostaining of apoptin protein showed that in can express in breast carcinoma cell lines after 12λ ZAP-CMV vector have not any apoptosis after 36 h as same as untreated cells.

Article Snippet: Large scale NBPs containing λ ZAP-CMV-apoptin expressing vector was conducted in 1 l of M9 medium (Sigma-Aldrich, USA) containing ampicillin (Sigma, USA).

Techniques: Immunostaining, Plasmid Preparation

BT-474, SKBR-3 and ZR-75 cells were examined after 36 h of transfection by flowcytometry. All the cell lines were susceptible to NBPs apoptin-induced apoptosis. We have not apoptosis in vector treated group and untreated group.

Journal: PLoS ONE

Article Title: λ Phage Nanobioparticle Expressing Apoptin Efficiently Suppress Human Breast Carcinoma Tumor Growth In Vivo

doi: 10.1371/journal.pone.0079907

Figure Lengend Snippet: BT-474, SKBR-3 and ZR-75 cells were examined after 36 h of transfection by flowcytometry. All the cell lines were susceptible to NBPs apoptin-induced apoptosis. We have not apoptosis in vector treated group and untreated group.

Article Snippet: Large scale NBPs containing λ ZAP-CMV-apoptin expressing vector was conducted in 1 l of M9 medium (Sigma-Aldrich, USA) containing ampicillin (Sigma, USA).

Techniques: Transfection, Plasmid Preparation

BT-474 breast carcinoma cell line transfected with λ ZAP-CMV-apoptin, λ ZAP-CMV vector and λ phage (vehicle) construct stained with FITC immunostaining and then visualized by fluorescence microscopy. There was no sign of cell necrosis after the treatments. There is only apoptotic morphology of cells after treatment with λ ZAP-CMV-apoptin.

Journal: PLoS ONE

Article Title: λ Phage Nanobioparticle Expressing Apoptin Efficiently Suppress Human Breast Carcinoma Tumor Growth In Vivo

doi: 10.1371/journal.pone.0079907

Figure Lengend Snippet: BT-474 breast carcinoma cell line transfected with λ ZAP-CMV-apoptin, λ ZAP-CMV vector and λ phage (vehicle) construct stained with FITC immunostaining and then visualized by fluorescence microscopy. There was no sign of cell necrosis after the treatments. There is only apoptotic morphology of cells after treatment with λ ZAP-CMV-apoptin.

Article Snippet: Large scale NBPs containing λ ZAP-CMV-apoptin expressing vector was conducted in 1 l of M9 medium (Sigma-Aldrich, USA) containing ampicillin (Sigma, USA).

Techniques: Transfection, Plasmid Preparation, Construct, Staining, Immunostaining, Fluorescence, Microscopy

( A ) Histochemistry analysis of tumor tissue sections showing apoptotic changes. The untreated tumor tissue contains many dividing cells. After 96 h treatment with NBPs there are a few cells maintained in the tumor tissue that could proliferate. Tumor growth was markedly suppressed in the apoptin treated group, ( B ) Histological examination of other organs (brain and heart) in tumor bearing mice that is not involved in the pathological changes of BT-474 cells. There are no changes in morphology of the brain and/or heart tissues and they are as same as control groups.

Journal: PLoS ONE

Article Title: λ Phage Nanobioparticle Expressing Apoptin Efficiently Suppress Human Breast Carcinoma Tumor Growth In Vivo

doi: 10.1371/journal.pone.0079907

Figure Lengend Snippet: ( A ) Histochemistry analysis of tumor tissue sections showing apoptotic changes. The untreated tumor tissue contains many dividing cells. After 96 h treatment with NBPs there are a few cells maintained in the tumor tissue that could proliferate. Tumor growth was markedly suppressed in the apoptin treated group, ( B ) Histological examination of other organs (brain and heart) in tumor bearing mice that is not involved in the pathological changes of BT-474 cells. There are no changes in morphology of the brain and/or heart tissues and they are as same as control groups.

Article Snippet: Large scale NBPs containing λ ZAP-CMV-apoptin expressing vector was conducted in 1 l of M9 medium (Sigma-Aldrich, USA) containing ampicillin (Sigma, USA).

Techniques:

( A ) TUNEL analysis revealed that apoptin induces the apoptotic activity in neoplasms (Magnification: ×400), ( B ) Analysis of survival. Mice treated with NBPs survived longer than the mice in the other 2 groups and the mean survival of NBPs-infected mice were >90 days (p<0.005). Fifty days after the beginning of the treatment, 90% of the animals infected by NBPs were alive, while at this time 100% of vector treated mice and 100% of saline-treated mice had died. Tumor-bearing mice treated with saline had a mean survival of 50 days.

Journal: PLoS ONE

Article Title: λ Phage Nanobioparticle Expressing Apoptin Efficiently Suppress Human Breast Carcinoma Tumor Growth In Vivo

doi: 10.1371/journal.pone.0079907

Figure Lengend Snippet: ( A ) TUNEL analysis revealed that apoptin induces the apoptotic activity in neoplasms (Magnification: ×400), ( B ) Analysis of survival. Mice treated with NBPs survived longer than the mice in the other 2 groups and the mean survival of NBPs-infected mice were >90 days (p<0.005). Fifty days after the beginning of the treatment, 90% of the animals infected by NBPs were alive, while at this time 100% of vector treated mice and 100% of saline-treated mice had died. Tumor-bearing mice treated with saline had a mean survival of 50 days.

Article Snippet: Large scale NBPs containing λ ZAP-CMV-apoptin expressing vector was conducted in 1 l of M9 medium (Sigma-Aldrich, USA) containing ampicillin (Sigma, USA).

Techniques: TUNEL Assay, Activity Assay, Infection, Plasmid Preparation