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Becton Dickinson λ triplex2 vector
Primers used for cloning and analysis of ligation mixtures.
λ Triplex2 Vector, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lambda+triplex2+recombinants/lambda+triplex2+recombinants/pmc01190383-33-8-11
Average 90 stars, based on 1 article reviews
λ triplex2 vector - by Bioz Stars, 2026-10
90/100 stars

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Article Title: Analyzing ligation mixtures using a PCR based method

Journal: Biological Procedures Online

doi: 10.1251/bpo108

Primers used for cloning and analysis of ligation mixtures.
Figure Legend Snippet: Primers used for cloning and analysis of ligation mixtures.

Techniques Used: Clone Assay, Ligation, Sequencing

Related Articles

Bacteria:

Article Title: Identification of Fer Tyrosine Kinase Localized on Microtubules as a Platelet Endothelial Cell Adhesion Molecule-1 Phosphorylating Kinase in Vascular Endothelial Cells
Article Snippet: .. Then, resuspended bacteria were infected with the lambda phage library containing human placental cDNAs (TriplEx2; BD Biosciences Clontech). ..

Infection:

Article Title: Identification of Fer Tyrosine Kinase Localized on Microtubules as a Platelet Endothelial Cell Adhesion Molecule-1 Phosphorylating Kinase in Vascular Endothelial Cells
Article Snippet: .. Then, resuspended bacteria were infected with the lambda phage library containing human placental cDNAs (TriplEx2; BD Biosciences Clontech). ..

Sequencing:

Article Title: Catalytic Efficiency Diversification of Duplicate β-1,3-1,4-Glucanases from Neocallimastix patriciarum J11
Article Snippet: .. The cDNA plasmids used for directional sequencing and sequential cloning were converted by in vivo excision from the lambda TriplEx2 recombinants in E. coli BM25.8 (BD Bioscience). ..

Article Title: Catalytic Efficiency Diversification of Duplicate ?-1,3-1,4-Glucanases from Neocallimastix patriciarum J11
Article Snippet: .. The cDNA plasmids used for directional sequencing and sequential cloning were converted by in vivo excision from the lambda TriplEx2 recombinants in E. coli BM25.8 (BD Bioscience). ..

Article Title: Analyzing ligation mixtures using a PCR based method
Article Snippet: .. The original cDNA library was constructed in a λ TriplEx2 vector (BD Bioscience/Clontech, Palo Alto, CA, USA) and the 60S-L37 and 40S-L17P inserts were generated as PCR products using vector based primers 5’ λ TriplEx2 sequencing primer and λ TriplEx3’ LD-insert screening amplimer (Fig. A and Table ). ..

Cloning:

Article Title: Catalytic Efficiency Diversification of Duplicate β-1,3-1,4-Glucanases from Neocallimastix patriciarum J11
Article Snippet: .. The cDNA plasmids used for directional sequencing and sequential cloning were converted by in vivo excision from the lambda TriplEx2 recombinants in E. coli BM25.8 (BD Bioscience). ..

Article Title: Catalytic Efficiency Diversification of Duplicate ?-1,3-1,4-Glucanases from Neocallimastix patriciarum J11
Article Snippet: .. The cDNA plasmids used for directional sequencing and sequential cloning were converted by in vivo excision from the lambda TriplEx2 recombinants in E. coli BM25.8 (BD Bioscience). ..

In Vivo:

Article Title: Catalytic Efficiency Diversification of Duplicate β-1,3-1,4-Glucanases from Neocallimastix patriciarum J11
Article Snippet: .. The cDNA plasmids used for directional sequencing and sequential cloning were converted by in vivo excision from the lambda TriplEx2 recombinants in E. coli BM25.8 (BD Bioscience). ..

Article Title: Catalytic Efficiency Diversification of Duplicate ?-1,3-1,4-Glucanases from Neocallimastix patriciarum J11
Article Snippet: .. The cDNA plasmids used for directional sequencing and sequential cloning were converted by in vivo excision from the lambda TriplEx2 recombinants in E. coli BM25.8 (BD Bioscience). ..

Isolation:

Article Title: Axial (apical-basal) expression of pro-apoptotic and pro-survival genes in the lake baikal demosponge Lubomirskia baicalensis.
Article Snippet: Like in all other Metazoa, also in sponges (Porifera) proliferation, differentiation, and death of cells are controlled by apoptotic processes, thus allowing the establishment of a Bauplan (body plan).. The demosponge Lubomirskia baicalensis from the Lake Baikal is especially suitable to assess the role of the apoptotic molecules, since its grade of construction is highly elaborated into an encrusting base and branches composed of modules lined up along the apical–basal axis.. The four cDNAs, ALG-2, BAK, MA-3, and Bcl-2, were isolated from this sponge species.

Membrane:

Article Title: Axial (apical-basal) expression of pro-apoptotic and pro-survival genes in the lake baikal demosponge Lubomirskia baicalensis.
Article Snippet: Like in all other Metazoa, also in sponges (Porifera) proliferation, differentiation, and death of cells are controlled by apoptotic processes, thus allowing the establishment of a Bauplan (body plan).. The demosponge Lubomirskia baicalensis from the Lake Baikal is especially suitable to assess the role of the apoptotic molecules, since its grade of construction is highly elaborated into an encrusting base and branches composed of modules lined up along the apical–basal axis.. The four cDNAs, ALG-2, BAK, MA-3, and Bcl-2, were isolated from this sponge species.

Polymerase Chain Reaction:

Article Title: Axial (apical-basal) expression of pro-apoptotic and pro-survival genes in the lake baikal demosponge Lubomirskia baicalensis.
Article Snippet: Like in all other Metazoa, also in sponges (Porifera) proliferation, differentiation, and death of cells are controlled by apoptotic processes, thus allowing the establishment of a Bauplan (body plan).. The demosponge Lubomirskia baicalensis from the Lake Baikal is especially suitable to assess the role of the apoptotic molecules, since its grade of construction is highly elaborated into an encrusting base and branches composed of modules lined up along the apical–basal axis.. The four cDNAs, ALG-2, BAK, MA-3, and Bcl-2, were isolated from this sponge species.

Article Title: Analyzing ligation mixtures using a PCR based method
Article Snippet: .. The original cDNA library was constructed in a λ TriplEx2 vector (BD Bioscience/Clontech, Palo Alto, CA, USA) and the 60S-L37 and 40S-L17P inserts were generated as PCR products using vector based primers 5’ λ TriplEx2 sequencing primer and λ TriplEx3’ LD-insert screening amplimer (Fig. A and Table ). ..

Caspase Assay:

Article Title: Axial (apical-basal) expression of pro-apoptotic and pro-survival genes in the lake baikal demosponge Lubomirskia baicalensis.
Article Snippet: Like in all other Metazoa, also in sponges (Porifera) proliferation, differentiation, and death of cells are controlled by apoptotic processes, thus allowing the establishment of a Bauplan (body plan).. The demosponge Lubomirskia baicalensis from the Lake Baikal is especially suitable to assess the role of the apoptotic molecules, since its grade of construction is highly elaborated into an encrusting base and branches composed of modules lined up along the apical–basal axis.. The four cDNAs, ALG-2, BAK, MA-3, and Bcl-2, were isolated from this sponge species.

cDNA Library Assay:

Article Title: Analyzing ligation mixtures using a PCR based method
Article Snippet: .. The original cDNA library was constructed in a λ TriplEx2 vector (BD Bioscience/Clontech, Palo Alto, CA, USA) and the 60S-L37 and 40S-L17P inserts were generated as PCR products using vector based primers 5’ λ TriplEx2 sequencing primer and λ TriplEx3’ LD-insert screening amplimer (Fig. A and Table ). ..

Construct:

Article Title: Analyzing ligation mixtures using a PCR based method
Article Snippet: .. The original cDNA library was constructed in a λ TriplEx2 vector (BD Bioscience/Clontech, Palo Alto, CA, USA) and the 60S-L37 and 40S-L17P inserts were generated as PCR products using vector based primers 5’ λ TriplEx2 sequencing primer and λ TriplEx3’ LD-insert screening amplimer (Fig. A and Table ). ..

Generated:

Article Title: Analyzing ligation mixtures using a PCR based method
Article Snippet: .. The original cDNA library was constructed in a λ TriplEx2 vector (BD Bioscience/Clontech, Palo Alto, CA, USA) and the 60S-L37 and 40S-L17P inserts were generated as PCR products using vector based primers 5’ λ TriplEx2 sequencing primer and λ TriplEx3’ LD-insert screening amplimer (Fig. A and Table ). ..



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