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lambda stacks acquired on the positive controls for nb and bc  (Carl Zeiss)


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    Structured Review

    Carl Zeiss lambda stacks acquired on the positive controls for nb and bc
    ( A , B) UMAP clustering based on spatial target distribution for both the NB ( A ) and BC ( B ) PDO biobank, n = 3 independent experiments. ( C , D ), Stacked bar graphs representing the proportion of each cluster per PDO line of the NB ( C ) and BC ( D ) PDO biobank, n = 3 independent experiments. ( E ), Representative 3D multispectral images showing heterogeneous binding of selected FGS probes on individual organoids within the same PDO line. Scale bars 20 μm for representative image of 000IJY, 30 μm for NBL39 and 15 μm for representative images of <t>13T</t> and 62T.
    Lambda Stacks Acquired On The Positive Controls For Nb And Bc, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lambda+stacks/confocal+disk+microscope+spinning/pmc11251264-137-19-32
    Average 90 stars, based on 1 article reviews
    lambda stacks acquired on the positive controls for nb and bc - by Bioz Stars, 2026-11
    90/100 stars

    Images

    1) Product Images from "A multispectral 3D live organoid imaging platform to screen probes for fluorescence guided surgery"

    Article Title: A multispectral 3D live organoid imaging platform to screen probes for fluorescence guided surgery

    Journal: EMBO Molecular Medicine

    doi: 10.1038/s44321-024-00084-4

    ( A , B) UMAP clustering based on spatial target distribution for both the NB ( A ) and BC ( B ) PDO biobank, n = 3 independent experiments. ( C , D ), Stacked bar graphs representing the proportion of each cluster per PDO line of the NB ( C ) and BC ( D ) PDO biobank, n = 3 independent experiments. ( E ), Representative 3D multispectral images showing heterogeneous binding of selected FGS probes on individual organoids within the same PDO line. Scale bars 20 μm for representative image of 000IJY, 30 μm for NBL39 and 15 μm for representative images of 13T and 62T.
    Figure Legend Snippet: ( A , B) UMAP clustering based on spatial target distribution for both the NB ( A ) and BC ( B ) PDO biobank, n = 3 independent experiments. ( C , D ), Stacked bar graphs representing the proportion of each cluster per PDO line of the NB ( C ) and BC ( D ) PDO biobank, n = 3 independent experiments. ( E ), Representative 3D multispectral images showing heterogeneous binding of selected FGS probes on individual organoids within the same PDO line. Scale bars 20 μm for representative image of 000IJY, 30 μm for NBL39 and 15 μm for representative images of 13T and 62T.

    Techniques Used: Binding Assay

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    Microscopy:

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    Fluorescence:

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    Imaging:

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    Spinning Disk:

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    Confocal:

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    Article Snippet: Lastly, cells were washed three times with 1× PBS, stained with DAPI, and mounted with ProLong Glass Antifade Mountant (Invitrogen). .. Slides were left curing in the dark O/N at RT and eventually imaged with a Zeiss Spinning Disk confocal microscope. ..



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    Image Search Results


    ( A , B) UMAP clustering based on spatial target distribution for both the NB ( A ) and BC ( B ) PDO biobank, n = 3 independent experiments. ( C , D ), Stacked bar graphs representing the proportion of each cluster per PDO line of the NB ( C ) and BC ( D ) PDO biobank, n = 3 independent experiments. ( E ), Representative 3D multispectral images showing heterogeneous binding of selected FGS probes on individual organoids within the same PDO line. Scale bars 20 μm for representative image of 000IJY, 30 μm for NBL39 and 15 μm for representative images of 13T and 62T.

    Journal: EMBO Molecular Medicine

    Article Title: A multispectral 3D live organoid imaging platform to screen probes for fluorescence guided surgery

    doi: 10.1038/s44321-024-00084-4

    Figure Lengend Snippet: ( A , B) UMAP clustering based on spatial target distribution for both the NB ( A ) and BC ( B ) PDO biobank, n = 3 independent experiments. ( C , D ), Stacked bar graphs representing the proportion of each cluster per PDO line of the NB ( C ) and BC ( D ) PDO biobank, n = 3 independent experiments. ( E ), Representative 3D multispectral images showing heterogeneous binding of selected FGS probes on individual organoids within the same PDO line. Scale bars 20 μm for representative image of 000IJY, 30 μm for NBL39 and 15 μm for representative images of 13T and 62T.

    Article Snippet: Linear unmixing accuracy was assessed by unmixing 32 channel lambda stacks acquired on the positive controls for NB and BC (respectively, KCNR and 13T), as described previously (van Ineveld et al, ) (Zeiss ZEN Blue).

    Techniques: Binding Assay