lambda gt10 primer (Promega)
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Promega
lambda gt10 primer
Lambda Gt10 Primer, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lambda+phosphatase/alkaline+phosphatase+treated+lambda+gt10+arms/pm09551399-41-10-13
Average 90 stars, based on 1 article reviews
Lambda Gt10 Primer, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lambda+phosphatase/alkaline+phosphatase+treated+lambda+gt10+arms/pm09551399-41-10-13
Average 90 stars, based on 1 article reviews
lambda gt10 primer - by Bioz Stars,
2026-11
90/100 stars
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Purification:Article Title: Cloning and sequencing of hydroxylase genes involved in taxol biosynthesis. Article Snippet: Two full-length cDNAs (TCH1 and TCH2) were obtained from a cDNA library of Taxus chinensis mainly by the single specific-primer PCR (SSP-PCR) method.. Compared with other reported enzymes from Taxus species, the deduced amino acid sequences of TCH1 and TCH2 exhibit significant homologies to hydroxylases that are involved in taxol biosynthesis.. These findings imply that the two new genes are closely related to the biosynthesis of taxol/taxoids. Article Title: Cloning and expression of Taxus acyltransferase cDNA. Article Snippet: A new full-length acyltransferase cDNA was obtained from Taxus chinensis by homologybased cloning strategy.. The cDNA has an open-reading frame of 1,275 nucleotides, which encodes 425 amino acids with a calculated molecular weight of 47,241 Da and an estimated pI value of 5.93.. The deduced amino acid sequence resembles the sequences of other cloned acyltransferases (56Ð61% identity; 71Ð75% similarity) involved directly in taxol biosynthetic pathways. cDNA Library Assay:Article Title: Cloning and sequencing of hydroxylase genes involved in taxol biosynthesis. Article Snippet: Two full-length cDNAs (TCH1 and TCH2) were obtained from a cDNA library of Taxus chinensis mainly by the single specific-primer PCR (SSP-PCR) method.. Compared with other reported enzymes from Taxus species, the deduced amino acid sequences of TCH1 and TCH2 exhibit significant homologies to hydroxylases that are involved in taxol biosynthesis.. These findings imply that the two new genes are closely related to the biosynthesis of taxol/taxoids. Article Title: Cloning and expression of Taxus acyltransferase cDNA. Article Snippet: A new full-length acyltransferase cDNA was obtained from Taxus chinensis by homologybased cloning strategy.. The cDNA has an open-reading frame of 1,275 nucleotides, which encodes 425 amino acids with a calculated molecular weight of 47,241 Da and an estimated pI value of 5.93.. The deduced amino acid sequence resembles the sequences of other cloned acyltransferases (56Ð61% identity; 71Ð75% similarity) involved directly in taxol biosynthetic pathways. Construct:Article Title: Cloning and sequencing of hydroxylase genes involved in taxol biosynthesis. Article Snippet: Two full-length cDNAs (TCH1 and TCH2) were obtained from a cDNA library of Taxus chinensis mainly by the single specific-primer PCR (SSP-PCR) method.. Compared with other reported enzymes from Taxus species, the deduced amino acid sequences of TCH1 and TCH2 exhibit significant homologies to hydroxylases that are involved in taxol biosynthesis.. These findings imply that the two new genes are closely related to the biosynthesis of taxol/taxoids. Article Title: Cloning and expression of Taxus acyltransferase cDNA. Article Snippet: A new full-length acyltransferase cDNA was obtained from Taxus chinensis by homologybased cloning strategy.. The cDNA has an open-reading frame of 1,275 nucleotides, which encodes 425 amino acids with a calculated molecular weight of 47,241 Da and an estimated pI value of 5.93.. The deduced amino acid sequence resembles the sequences of other cloned acyltransferases (56Ð61% identity; 71Ð75% similarity) involved directly in taxol biosynthetic pathways. Article Title: Salt- and osmotic stress-induced choline monooxygenase expression in Kochia scoparia is ABA-independent Article Snippet: Choline monooxygenase (CMO) is the first regulatory enzyme in the biosynthetic pathway for glycine betaine, an effective osmoprotectant in Kochia scoparia, a highly droughtand salt-tolerant species.. In seedlings, CMO transcript levels are rapidly increased in response to both NaCl and osmotic stress treatments.. The mRNA level in shoots was substantially higher than in roots. Polymerase Chain Reaction:Article Title: Development of 52 new polymorphic microsatellite markers for the olive flounder, Paralichthys olivaceus. Article Snippet: We characterized 52 new microsatellite markers isolated from (GT)n and (CT)n microsatelliteenriched genomic libraries of the olive flounder (Paralichthys olivaceus).. All markers were polymorphic, with eight to 30 (mean 15.1) alleles detected in 30 individuals from a single natural population.. Observed heterozygosity ranged from 0.20 to 1.0. Amplification:Article Title: Development of 52 new polymorphic microsatellite markers for the olive flounder, Paralichthys olivaceus. Article Snippet: We characterized 52 new microsatellite markers isolated from (GT)n and (CT)n microsatelliteenriched genomic libraries of the olive flounder (Paralichthys olivaceus).. All markers were polymorphic, with eight to 30 (mean 15.1) alleles detected in 30 individuals from a single natural population.. Observed heterozygosity ranged from 0.20 to 1.0. Magnetic Beads:Article Title: Development of 52 new polymorphic microsatellite markers for the olive flounder, Paralichthys olivaceus. Article Snippet: We characterized 52 new microsatellite markers isolated from (GT)n and (CT)n microsatelliteenriched genomic libraries of the olive flounder (Paralichthys olivaceus).. All markers were polymorphic, with eight to 30 (mean 15.1) alleles detected in 30 individuals from a single natural population.. Observed heterozygosity ranged from 0.20 to 1.0. other:Article Title: Isolation and characterization of polymorphic microsatellite DNA markers for an endangered freshwater fish in Korea, the bull-head torrent catfish Liobagrus obesus Article Snippet: The bull-head torrent catfish Liobagrus obesus (family Amblycipitidae) is an endangered freshwater fish in Korea.. There has been a rapid decline in its population, caused by anthropogenic activities.. This may also lead to reduced genetic diversity, which can significantly affect survival. Article Title: Peptide vaccination is superior to genetic vaccination using a recombineered bacteriophage λ subunit vaccine. Article Snippet: Genetic immunization holds promise as a vaccination method, but has so far proven ineffective in large primate and human trials.. Herein, we examined the relative merits of genetic immunization and peptide immunization using bacteriophage .. Bacteriophage has proven effective in immune challenge models using both immunization methods, but there has never been a direct comparison of efficacy and of the quality of immune response. Article Title: Nucleic acids encoding a house dust mite allergen Article Snippet: After the addition of EcoRI restriction enzyme linkers (New England Biolabs, Beverly, U.S.A.), the cDNA was ligated to Article Title: Nucleic acids encoding a house dust mite allergen, Article Snippet: After the addition of EcoRI restriction enzyme linkers (New England Biolabs, Beverly, U.S.A.), the cDNA was ligated to |
![(A) Representative confocal images of axon with growth cones stained for tyrosinated α-tubulin (Tyr, red), F-actin (phalloidin, magenta), and phosphorylated-VASH1 (P[S337]VASH1, gray). Scale bar, 10 µm. ( B ) Quantification of P[S337]VASH1 in growth cones of WT and Vash1 KO neurons. n/N: 67/5 (WT), 111/7 (KO) with n indicating the number of growth cones and N of E13 embryos. Data are expressed as mean ± SD and as fold change over WT. ( C ) Quantification of P[S337]VASH1 in growth cones without treatment or with CNP treatment. n/N: 61/4 (control), 48/3 (CNP) with n indicating the number of growth cones and N the number of E13 embryos. Data are expressed as mean ± SD and as fold change over control. Statistical significance was determined using Mann-Witney test. **** p < 0.0001, ns p > 0.05. ( D, E ) Detection by immunoblot of VASH1 protein and its phosphorylated form in cultured DRG neurons after immunoprecipitation using an anti-SVBP antibody. β-tubulin staining shows similar protein content in the immunoprecipitation inputs. ( D ) Both VASH1 protein and its phosphorylated form are detected in WT but not in Vash1 KO DRGs. Treatment with <t>Lambda</t> protein <t>phosphatase</t> results in the loss of P[S337]VASH1 band. ( E, F ) CNP induces phosphorylation of S337 of VASH1, which is reversed by DT-3, as shown by representative immunoblots from an experiment done with cultured DRG explants ( E ) and quantifications of the P[S337]VASH1/VASH1 ratios from 4 independent experiments ( F ). In F , triangles correspond to data from immunoprecipitation experiments done with cultured DRG dissociated neurons, and circle to immunoprecipitation experiments done with cultured DRG explants.](https://bio-rxiv-images-cdn.bioz.com/dois_ending_with_41/10__64898_slash_2026__10__14__759141/10__64898_slash_2026__10__14__759141___F6.large.jpg)