lambda phosphatase λpp buffer (New England Biolabs)

New England Biolabs is a verified supplier

New England Biolabs manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

New England Biolabs lambda phosphatase λpp buffer

( A ) 2D gel western blots (αGFP) of <t>lambda</t> <t>phosphatase</t> <t>(λPP)-treated</t> protein extracts from adult soil-grown plants expressing bZIP63-GFP (ox#3). ( B ) Phos-tag gel western blots showing the in vivo phosphorylation state of bZIP63 in seedlings after 6 hr extended night in the presence (+) or absence (−) of 1% sucrose and in 5 week-old soil-grown plants after 6 hr light (L) or extended night (EN). Plants either expressed 35S::bZIP63-GFP (ox#3) or a genomic fragment of bZIP63 (GY11) with a YFP-tag. Recombinant bZIP63-YFP was used as a nonphosphorylated control. Numbered arrowheads on the right mark the position of each observed bZIP63 band for easy reference with other figures (see for a comparative image of all Phos-tag western blots). For more information on the genomic line see and . ( C ) Identification of in vivo phosphorylation sites by immunoprecipitation (IP) and tandem mass spectrometry (MS/MS). An exemplary western blot of the IP is shown. The scheme at the bottom shows the positions of the identified in vivo phosphorylation sites and the total sequence coverage reached with each proteolytic enzyme (grey bars). Asterisks mark the identification of a phospho-site. For more information on samples and (phospho-) peptides see . IEF, isoelectric focusing; CBB, coomassie brilliant blue; BD, basic domain; ZIP, leucine zipper; LTQ, linear ion trap quadrupole; chym., chymotrypsin. DOI: http://dx.doi.org/10.7554/eLife.05828.012

Lambda Phosphatase λpp Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 2943 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lambda+phosphatase+%CE%BBpp+buffer/Lambda+Protein+Phosphatase/pmc04558565-617-4-8
Average 99 stars, based on 2943 article reviews

lambda phosphatase λpp buffer - by Bioz Stars, 2026-11

99/100 stars

Images

1) Product Images from "SnRK1-triggered switch of bZIP63 dimerization mediates the low-energy response in plants"

Article Title: SnRK1-triggered switch of bZIP63 dimerization mediates the low-energy response in plants

Journal: eLife

doi: 10.7554/eLife.05828

( A ) 2D gel western blots (αGFP) of lambda phosphatase (λPP)-treated protein extracts from adult soil-grown plants expressing bZIP63-GFP (ox#3). ( B ) Phos-tag gel western blots showing the in vivo phosphorylation state of bZIP63 in seedlings after 6 hr extended night in the presence (+) or absence (−) of 1% sucrose and in 5 week-old soil-grown plants after 6 hr light (L) or extended night (EN). Plants either expressed 35S::bZIP63-GFP (ox#3) or a genomic fragment of bZIP63 (GY11) with a YFP-tag. Recombinant bZIP63-YFP was used as a nonphosphorylated control. Numbered arrowheads on the right mark the position of each observed bZIP63 band for easy reference with other figures (see for a comparative image of all Phos-tag western blots). For more information on the genomic line see and . ( C ) Identification of in vivo phosphorylation sites by immunoprecipitation (IP) and tandem mass spectrometry (MS/MS). An exemplary western blot of the IP is shown. The scheme at the bottom shows the positions of the identified in vivo phosphorylation sites and the total sequence coverage reached with each proteolytic enzyme (grey bars). Asterisks mark the identification of a phospho-site. For more information on samples and (phospho-) peptides see . IEF, isoelectric focusing; CBB, coomassie brilliant blue; BD, basic domain; ZIP, leucine zipper; LTQ, linear ion trap quadrupole; chym., chymotrypsin. DOI: http://dx.doi.org/10.7554/eLife.05828.012

Figure Legend Snippet: ( A ) 2D gel western blots (αGFP) of lambda phosphatase (λPP)-treated protein extracts from adult soil-grown plants expressing bZIP63-GFP (ox#3). ( B ) Phos-tag gel western blots showing the in vivo phosphorylation state of bZIP63 in seedlings after 6 hr extended night in the presence (+) or absence (−) of 1% sucrose and in 5 week-old soil-grown plants after 6 hr light (L) or extended night (EN). Plants either expressed 35S::bZIP63-GFP (ox#3) or a genomic fragment of bZIP63 (GY11) with a YFP-tag. Recombinant bZIP63-YFP was used as a nonphosphorylated control. Numbered arrowheads on the right mark the position of each observed bZIP63 band for easy reference with other figures (see for a comparative image of all Phos-tag western blots). For more information on the genomic line see and . ( C ) Identification of in vivo phosphorylation sites by immunoprecipitation (IP) and tandem mass spectrometry (MS/MS). An exemplary western blot of the IP is shown. The scheme at the bottom shows the positions of the identified in vivo phosphorylation sites and the total sequence coverage reached with each proteolytic enzyme (grey bars). Asterisks mark the identification of a phospho-site. For more information on samples and (phospho-) peptides see . IEF, isoelectric focusing; CBB, coomassie brilliant blue; BD, basic domain; ZIP, leucine zipper; LTQ, linear ion trap quadrupole; chym., chymotrypsin. DOI: http://dx.doi.org/10.7554/eLife.05828.012

Techniques Used: Two-Dimensional Gel Electrophoresis, Western Blot, Expressing, In Vivo, Phospho-proteomics, Recombinant, Control, Immunoprecipitation, Mass Spectrometry, Tandem Mass Spectroscopy, Sequencing

Incubation:

Article Title: AKT Phosphorylation of FOXK2 at Ser398 Impacts Breast Cancer Cell Growth and Viability
Article Snippet: .. Afterwards, 100 μg of protein were incubated in a total of 50 μL containing 400 units of lambda protein phosphatase (λPP; P0753S, New England BioLabs Inc., Ipswich, MA, USA), 1× PMP (NEBuffer Pack for Protein MetalloPhosphatases; B0761S, New England BioLabs Inc.) and 1 mM MnCl2 (B1761S, New England BioLabs Inc.) for 1 h at 37°C. ..

Article Title: Single-molecule resolution of post-translational modification on GRK4 with cyclodextrin/SiN x hybrid nanopore.
Article Snippet: .. A 100 μL of the mixture containing 10 μM of pep-T182-P and 200 U of lambda protein phosphatase in 1 mM MnCl2 and NEbuffer for PMP was incubated at 30 ◦C for 2 h, a control assay is under identical condition but without lambda protein phosphatase. ..

Article Title: Identification of NEK9 as a novel substrate of protein kinase B.
Article Snippet: Protein kinase B (PKB/Akt) regulates diverse cellular processes through phosphorylation of numerous substrate proteins downstream of phosphatidylinositol 3-kinase (PI3K).. Although many PKB substrates have been identified, additional substrates remain to be characterized.. NEK9 is an essential serine/threonine kinase involved in mitotic progression and contains two putative PKB consensus phosphorylation motifs.

Article Title: The PvAURK-PvGRFs regulatory module controls salt tolerance in common bean (Phaseolus vulgaris L.).
Article Snippet: .. For the dephosphorylation assay, the extracted protein samples were incubated with λ-protein phosphatase (λ-PPase, New England Biolabs) at 30 ◦C for 45 min according to the manufacturer's instructions. ..

Article Title: VASH1 phosphorylation regulates tubulin detyrosination via CNP/PRKG1α signaling in DRG neurons
Article Snippet: .. For phosphatase treatment experiments, bead-bound complexes were incubated with phosphatase (Lambda protein phosphatase, New England Biolabs) for 30 min at 30°C according to the manufacturer’s protocol before elution. ..

Control:

Thin Layer Chromatography:

Article Title: A bifunctional ARHGEF17-AS1 locus encodes MSEP, a protein that supports mitotic spindle organization.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-α-tubulin (clone DM1A) Sigma-Aldrich Cat# T6199; RRID: AB_477583 Rabbit polyclonal anti-α-tubulin Proteintech Cat# 11224-1-AP; RRID: AB_2210206 Mouse monoclonal anti-γ-tubulin (clone GTU-88) Sigma-Aldrich Cat# T5326; RRID: AB_532292 Rabbit polyclonal anti-γ-tubulin Sigma-Aldrich Cat# T5192; RRID: AB_261690 Mouse monoclonal anti-RPS3 (clone 2G7H4) Proteintech Cat# 66046-1-Ig; RRID: AB_11182493 Rabbit polyclonal anti-RPS3 Novus Biologicals Cat# NBP1-33691; RRID: AB_2180759 Rabbit monoclonal anti-RPS3 (clone D50G7) Cell Signaling Technology Cat# 9538S; RRID: AB_10622028 Rabbit polyclonal anti-RNF10 Proteintech Cat# 16936-1-AP; RRID: AB_2181846 Mouse monoclonal anti-TPX2 (clone 2E10C1) Proteintech Cat# 68712-1-Ig; RRID: AB_3670405 Rabbit polyclonal anti-TPX2 Proteintech Cat# 11741-1-AP; RRID: AB_2208895 Mouse monoclonal anti-KPNA2 (clone 2G9C1) Proteintech Cat# 66870-1-Ig; RRID: AB_2882207 Rabbit polyclonal anti-KPNA2 Proteintech Cat# 10819-1-AP; RRID: AB_2265526 Rabbit polyclonal anti-DYNC1H1/dynein heavy chain Proteintech Cat# 12345-1-AP; RRID: AB_2261765 Mouse monoclonal anti-cytoplasmic dynein intermediate chain (clone 74.1) Abcam Cat# ab23905; RRID: AB_2096669 Mouse monoclonal anti-FLAG M2 (clone M2) Sigma-Aldrich Cat# F1804; RRID: AB_262044 Mouse monoclonal anti-GFP tag (clone 1E10H7) Proteintech Cat# 66002-1-Ig; RRID: AB_11182611 Rabbit polyclonal anti-GFP Abcam Cat# ab290; RRID: AB_303395 Rabbit monoclonal anti-GAPDH (clone D16H11) Cell Signaling Technology Cat# 5174; RRID: AB_10622025 Rabbit monoclonal anti-Aurora-A/AIK (clone 1G4) Cell Signaling Technology Cat# 4718; RRID: AB_2061482 Mouse monoclonal anti-Aurora-A (clone 1F4B10) Proteintech Cat# 66757-1-Ig; RRID: AB_2882103 Rabbit monoclonal anti-WEE1 (clone D10D2) Cell Signaling Technology Cat# 13084; RRID: AB_2713924 Rabbit monoclonal anti-PLK1 (clone 208G4) Cell Signaling Technology Cat# 4513; RRID: AB_2167409 Rabbit polyclonal anti-vinculin Proteintech Cat# 26520-1-AP; RRID: AB_2868558 Normal mouse IgG MilliporeSigma Cat# 12–371; RRID: AB_145840 Normal rabbit IgG MilliporeSigma Cat# 12–370; RRID: AB_145841 Rabbit polyclonal anti-MSEP; immunogen CGHRPQRLHPGRP (residues 257–269) This paper; Innovagen AB N/A Anti-MYC tag antibody Abcam Cat# ab32; RRID: AB_303599 Anti-cyclin B1 antibody Abcam Cat# ab32053; RRID: AB_731779 Anti-total PARP antibody Abcam Cat# ab191217; RRID: AB_2861274 (Continued on next page) 16 Cell Reports 45, 118026, October 27, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER N-Acetyl-D-sphingosine; C2 ceramide (d18:1/2:0), ≥97% (TLC) Sigma-Aldrich Cat# A7191; CAS: 3102–57-6 Lambda Protein Phosphatase New England Biolabs Cat# P0753S Puromycin dihydrochloride Sigma-Aldrich Cat# P8833; CAS: 58–58-2 Hygromycin B Sigma-Aldrich Cat# H3274; CAS: 31282–04-9 TRIzol Reagent Invitrogen/Thermo Fisher Scientific Cat# 15596026 ProLong Gold Antifade Mountant Invitrogen/Thermo Fisher Scientific Cat# P36930 RNase A Invitrogen/Thermo Fisher Scientific Cat# 12091021 Halt Protease and Phosphatase Inhibitor Cocktail Thermo Fisher Scientific Cat# 78440 Phosphatase Inhibitor Cocktail Set IV MilliporeSigma Cat# 524628 Protein G magnetic beads Invitrogen/Thermo Fisher Scientific Cat# 10004D MSEP immunogen peptide CGHRPQRLHPGRP (residues 257–269) This paper; Innovagen AB N/A Critical commercial assays Lipofectamine RNAiMAX Transfection Reagent Invitrogen/Thermo Fisher Scientific Cat# 13778150 Lipofectamine 2000 Transfection Reagent Invitrogen/Thermo Fisher Scientific Cat# 11668019 SF Cell Line 4D-Nucleofector X Kit L Lonza Cat# V4XC-2012 ReliaPrep RNA Cell Miniprep System Promega Cat# Z6010, Z6011. .. ImProm-II Reverse Transcription System Promega Cat# A3800 Power SYBR Green PCR Master Mix Applied Biosystems/Thermo Fisher Scientific Cat# 4367659 RNAscope Multiplex Fluorescent Reagent Kit v2 Advanced Cell Diagnostics/Bio-Techne Cat# 323100 Duolink In Situ Red Starter Kit Mouse/Rabbit MilliporeSigma Cat# DUO92101 Pierce BCA Protein Assay Kit Thermo Fisher Scientific Cat# 23225 NuPAGE Novex 4–12% Bis-Tris protein gels Invitrogen/Thermo Fisher Scientific Cat# NP0321 Microcon 30 kDa centrifugal filter units Merck Millipore Cat# MRCF0R030 CellTiter-Glo 2.0 Cell Viability Assay Promega Cat# G9241 Deposited data RNA-sequencing data reported in this study This paper; NCBI Gene Expression Omnibus GEO: GSE274156; https://www.ncbi.nlm.

Review

lambda phosphatase λpp buffer (New England Biolabs)

Structured Review

Images

1) Product Images from "SnRK1-triggered switch of bZIP63 dimerization mediates the low-energy response in plants"

Related Articles

Incubation:

Control:

Thin Layer Chromatography:

Magnetic Beads:

Transfection:

De-Phosphorylation Assay:

Similar Products

Image Search Results