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lambda nuclease buffer  (New England Biolabs)


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    Structured Review

    New England Biolabs lambda nuclease buffer
    Lambda Nuclease Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 1100 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lambda+nuclease+buffer/Lambda+Exonuclease/us11266655-1507-17-32
    Average 98 stars, based on 1100 article reviews
    lambda nuclease buffer - by Bioz Stars, 2026-09
    98/100 stars

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    Incubation:

    Article Title: Novel Nuclease MbovP701 with a Yqaj Domain Is Interrelated with the Growth of Mycoplasma bovis
    Article Snippet: .. Subsequently, the two dsDNA substrates (1 μg) were separately incubated with 2.5 μg of Lambda-Exo (NEB, Beijing, China) or rMbovP701 in Tris-HCl buffer (25 mM, pH8.0), containing 7.5 mM MgCl 2 and 1 mM DTT at 37 °C. ..

    Lysis:

    Article Title: Interrogation of the interplay between DNA N 6 -methyladenosine (6mA) and hypoxia-induced chromatin accessibility by a randomized empirical model (EnrichShuf)
    Article Snippet: The DNA:antibody mixture was cross-linked using UV-C irradiation in 1 × IP buffer (10 mM Tris–HCl pH 7.4, 150 mM NaCl, 0.1% Igepal-630) and pulled down by protein A Dynabeads (Invitrogen). .. Subsequent washes were performed with FA lysis buffer (50 mM HEPES-KOH pH 7.4, 150 mM NaCl, 2 mM EDTA, 1% Triton X-100, 0.1% sodium deoxycholate), NaCl buffer (50 mM HEPES-KOH pH 7.4, 500 mM NaCl, 2 mM EDTA, 1% Triton X-100, 0.1% sodium deoxycholate), LiCl buffer (100 mM Tris–HCl pH 7.9, 500 mM LiCl, 1% NP-40, 1% sodium deoxycholate), TE buffer (10 mM Tris–HCl pH 7.4, 1 mM EDTA) and Tris buffer (10 mM Tris–HCl pH 7.4) in the following enzymatic steps: DNA repair with PreCR Repair mix (M0309S, NEB), A-tailing with Klenow fragment (3′–5′, exo-) (M0212S, NEB), ligating the adapter with T4 ligase (M0202S, NEB) and T4 polynucleotide kinase (M0201S, NEB), fill-in with phi29 DNA polymerase (M0269S, NEB) and digesting with lambda exonuclease (M0262S, NEB). ..

    Polymerase Chain Reaction:

    Article Title: Gut epithelium modifies enteric behaviors during nutritional adversity via distinct peptidergic signaling axes
    Article Snippet: .. 10 μg of the PCR product was digested with lambda exonuclease (NEB) to digest the DNA strand with 5’ phosphorylation. .. The unphosphorylated strand of DNA was purified using Monarch PCR & DNA Cleanup Kit (NEB) and was used as a repair template at a final concentration of 100 ng/μL in the CRISPR injection mix.

    Article Title: Meiotic crossovers revealed by differential visualization of homologous chromosomes using enhanced haplotype oligo-painting in cucumber.
    Article Snippet: .. Two units of lambda exonuclease (M0262; New England Biolabs, Ipswich, MA, USA) were added to the two 50 lL PCR reactions obtained, respectively. ..

    Phospho-proteomics:

    Article Title: Gut epithelium modifies enteric behaviors during nutritional adversity via distinct peptidergic signaling axes
    Article Snippet: .. 10 μg of the PCR product was digested with lambda exonuclease (NEB) to digest the DNA strand with 5’ phosphorylation. .. The unphosphorylated strand of DNA was purified using Monarch PCR & DNA Cleanup Kit (NEB) and was used as a repair template at a final concentration of 100 ng/μL in the CRISPR injection mix.



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