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lambda sc smart shutter controller unit  (Sutter Instrument Company)


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    Sutter Instrument Company lambda sc smart shutter controller unit
    Lambda Sc Smart Shutter Controller Unit, supplied by Sutter Instrument Company, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lambda+ls+with+smart+shutter/lambda+sc+smart+shutter+controllers/pm26312752-97-13-19
    Average 90 stars, based on 1 article reviews
    lambda sc smart shutter controller unit - by Bioz Stars, 2026-10
    90/100 stars

    Images

    Related Articles

    Generated:

    Article Title: High-Throughput All-Optical Analysis of Synaptic Transmission and Synaptic Vesicle Recycling in Caenorhabditis elegans.
    Article Snippet: Excitation light was passed through a double band pass excitation filter (479 and 585 nm), combined with a 605 nm beam splitter and a 647 nm emission filter (F74-423, F38-605, and F37-647, respectively; AHF Analysentechnik, Germany). .. Blue light illumination protocols (temporal sequences) were programmed on, and generated by, a Lambda SC Smart shutter controller unit (Sutter Instruments, USA), using its TTL output to drive the LED power supply. ..

    other:

    Article Title: Distinct fusion properties of synaptotagmin-1 and synaptotagmin-7 bearing dense core granules
    Article Snippet: The system uses Lambda SC smart-shutter controllers (Sutter Instruments, Novato, CA) that allow for rapid selection of three shutter openings sequentially— p - and s -polarized 561 nm excitation and the 488-nm excitation (one at a time)—and repeats the cycle using MetaMorph software (Molecular Devices, Sunnyvale, CA).

    Imaging:

    Article Title: A Microfluidic Device for Massively Parallel, Whole-lifespan Imaging of Single Fission Yeast Cells
    Article Snippet: Mini labroller (Labnet) Plasma Cleaner (Harrick Plasma, catalog number: PDC-32G) Laboratory oven (Ecotherm, Precision) Dissection microscope (AmScope, catalog number: SM-1T-PL) Fine tweezers (Fisher Scientific, catalog number: 16-100-103) Sonicator (Bransonic, catalog number: 2510R-DTH) Rocker/agitator (Belly Dancer; Stovall Life Science) Bunsen burner (accuFlame; Fisher Scientific, catalog number: 03-902Q) Centrifuge (Beckman Coulter, model: Avanti ® J-26XP) Centrifuge rotor (Beckman Coulter, model: JLA-16.250) .. list-behavior=enumerated prefix-word= mark-type=decimal max-label-size=0 Epifluorescence imaging microscope (Eclipse Ti; Nikon) Focus maintenance system (Nikon Perfect Focus, Nikon) CMOS camera (Andor, model: Zyla 5.5 sCMOS) 10x, 0.3 NA objective (Plan Fluor; Nikon) 60x, 0.95 NA objective (Plan Apo Lambda; Nikon) Computer-controlled microscope stage (Proscan III motorized stage; Prior) Objective heater (Bioptechs, catalog number: 150819-19) Appropriate filters for fluorescent imaging list-behavior=enumerated prefix-word= mark-type=lower-alpha max-label-size=0 eGFP (Chroma, catalog number: 49002) mKO (Chroma, catalog number: 49010) E2Crimson (Chroma, catalog number: 49015) Light source shutter (SmartShutter; Sutter Instrument) Shutter controller (Lambda SC; Sutter Instrument) Computer-controlled syringe pump (KD Scientific, model: LEGATO ® 210) Note: This pump is configured for two syringes. ..

    Article Title: Phosphatidylinositol 4-phosphate is a major source of GPCR-stimulated phosphoinositide production.
    Article Snippet: .. For TIRF microscopy imaging, we used Sutter Instrument Lambda SC SmartShutter controllers that enable rapid selection of two shutter openings sequentially, a 488-nm excitation (for GFP-P4M) and a 561-nm excitation (for FRB-mRFP), one at a time. .. The common beam path was focused through a custom side port to a sidefacing filter cube placed below the objective turret of an Olympus IX81 inverted microscope to produce objective-based TIRF illumination.

    Microscopy:

    Article Title: A Microfluidic Device for Massively Parallel, Whole-lifespan Imaging of Single Fission Yeast Cells
    Article Snippet: Mini labroller (Labnet) Plasma Cleaner (Harrick Plasma, catalog number: PDC-32G) Laboratory oven (Ecotherm, Precision) Dissection microscope (AmScope, catalog number: SM-1T-PL) Fine tweezers (Fisher Scientific, catalog number: 16-100-103) Sonicator (Bransonic, catalog number: 2510R-DTH) Rocker/agitator (Belly Dancer; Stovall Life Science) Bunsen burner (accuFlame; Fisher Scientific, catalog number: 03-902Q) Centrifuge (Beckman Coulter, model: Avanti ® J-26XP) Centrifuge rotor (Beckman Coulter, model: JLA-16.250) .. list-behavior=enumerated prefix-word= mark-type=decimal max-label-size=0 Epifluorescence imaging microscope (Eclipse Ti; Nikon) Focus maintenance system (Nikon Perfect Focus, Nikon) CMOS camera (Andor, model: Zyla 5.5 sCMOS) 10x, 0.3 NA objective (Plan Fluor; Nikon) 60x, 0.95 NA objective (Plan Apo Lambda; Nikon) Computer-controlled microscope stage (Proscan III motorized stage; Prior) Objective heater (Bioptechs, catalog number: 150819-19) Appropriate filters for fluorescent imaging list-behavior=enumerated prefix-word= mark-type=lower-alpha max-label-size=0 eGFP (Chroma, catalog number: 49002) mKO (Chroma, catalog number: 49010) E2Crimson (Chroma, catalog number: 49015) Light source shutter (SmartShutter; Sutter Instrument) Shutter controller (Lambda SC; Sutter Instrument) Computer-controlled syringe pump (KD Scientific, model: LEGATO ® 210) Note: This pump is configured for two syringes. ..

    Article Title: Phosphatidylinositol 4-phosphate is a major source of GPCR-stimulated phosphoinositide production.
    Article Snippet: .. For TIRF microscopy imaging, we used Sutter Instrument Lambda SC SmartShutter controllers that enable rapid selection of two shutter openings sequentially, a 488-nm excitation (for GFP-P4M) and a 561-nm excitation (for FRB-mRFP), one at a time. .. The common beam path was focused through a custom side port to a sidefacing filter cube placed below the objective turret of an Olympus IX81 inverted microscope to produce objective-based TIRF illumination.

    Selection:

    Article Title: Phosphatidylinositol 4-phosphate is a major source of GPCR-stimulated phosphoinositide production.
    Article Snippet: .. For TIRF microscopy imaging, we used Sutter Instrument Lambda SC SmartShutter controllers that enable rapid selection of two shutter openings sequentially, a 488-nm excitation (for GFP-P4M) and a 561-nm excitation (for FRB-mRFP), one at a time. .. The common beam path was focused through a custom side port to a sidefacing filter cube placed below the objective turret of an Olympus IX81 inverted microscope to produce objective-based TIRF illumination.



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    Image Search Results


    Cell unroofing is useful for a variety of adherent cell types. (A) Cartoon of unroofing approach in which cells are stained with R18 (red) after sonication. (B) Unroofed and stained ND7/23, PC-12, F-11, and HEK293T/17 cells imaged with epifluorescence microscopy. Scale bar applies to all images and is 50 µm.

    Journal: The Journal of General Physiology

    Article Title: Transition metal ion FRET to measure short-range distances at the intracellular surface of the plasma membrane

    doi: 10.1085/jgp.201511530

    Figure Lengend Snippet: Cell unroofing is useful for a variety of adherent cell types. (A) Cartoon of unroofing approach in which cells are stained with R18 (red) after sonication. (B) Unroofed and stained ND7/23, PC-12, F-11, and HEK293T/17 cells imaged with epifluorescence microscopy. Scale bar applies to all images and is 50 µm.

    Article Snippet: To label, patches were perfused with 100–250 nM R18 in recording solution (130 mM NaCl, 3 mM HEPES, and 0.2 mM EDTA, pH 7.2) for 1–2 min. To monitor labeling, patches were excited with epifluorescence (Lambda LS with Smart Shutter; Sutter Instrument) and a 560/10-nm excitation filter (Chroma Technology Corp.) and imaged with a 615/60 emission filter (Chroma Technology Corp.) until fluorescence of the patch reached steady state.

    Techniques: Staining, Sonication, Epifluorescence Microscopy

    tmFRET between R18 and Co 2+ -C18-NTA in inside-out patches from a Xenopus oocyte. (A) Bright-field and epifluorescence images from a representative patch. Fluorescence images from patches with either 0 or 1 µM Co 2+ before (left) or after (middle) application of C18-NTA and after 20 mM EDTA (right). Scale bar in top left image applies to all images and is 20 µm. (B) Patch fluorescence in Co 2+ was normalized to patch fluorescence in EDTA before and after C18-NTA ( n = 4). Mean (lines) and individual (points) patch values are shown. Asterisk indicates significance at P < 0.05.

    Journal: The Journal of General Physiology

    Article Title: Transition metal ion FRET to measure short-range distances at the intracellular surface of the plasma membrane

    doi: 10.1085/jgp.201511530

    Figure Lengend Snippet: tmFRET between R18 and Co 2+ -C18-NTA in inside-out patches from a Xenopus oocyte. (A) Bright-field and epifluorescence images from a representative patch. Fluorescence images from patches with either 0 or 1 µM Co 2+ before (left) or after (middle) application of C18-NTA and after 20 mM EDTA (right). Scale bar in top left image applies to all images and is 20 µm. (B) Patch fluorescence in Co 2+ was normalized to patch fluorescence in EDTA before and after C18-NTA ( n = 4). Mean (lines) and individual (points) patch values are shown. Asterisk indicates significance at P < 0.05.

    Article Snippet: To label, patches were perfused with 100–250 nM R18 in recording solution (130 mM NaCl, 3 mM HEPES, and 0.2 mM EDTA, pH 7.2) for 1–2 min. To monitor labeling, patches were excited with epifluorescence (Lambda LS with Smart Shutter; Sutter Instrument) and a 560/10-nm excitation filter (Chroma Technology Corp.) and imaged with a 615/60 emission filter (Chroma Technology Corp.) until fluorescence of the patch reached steady state.

    Techniques: Fluorescence