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phage lambda dna  (New England Biolabs)


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    Structured Review

    New England Biolabs phage lambda dna
    Templates and primers.
    Phage Lambda Dna, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1829 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lambda+dna/Lambda+DNA/pmc07841393-8-5-8
    Average 97 stars, based on 1829 article reviews
    phage lambda dna - by Bioz Stars, 2026-09
    97/100 stars

    Images

    1) Product Images from "A Single Amino Acid Change to Taq DNA Polymerase Enables Faster PCR, Reverse Transcription and Strand-Displacement"

    Article Title: A Single Amino Acid Change to Taq DNA Polymerase Enables Faster PCR, Reverse Transcription and Strand-Displacement

    Journal: Frontiers in Bioengineering and Biotechnology

    doi: 10.3389/fbioe.2020.553474

    Templates and primers.
    Figure Legend Snippet: Templates and primers.

    Techniques Used: Lamp Assay, Lambda DNA Preparation, Reverse Transcription Polymerase Chain Reaction

    Related Articles

    Construct:

    Article Title: Hidden route of protein damage through oxygen-confined photooxidation
    Article Snippet: .. The DNA constructs, modified at one end with amine group and at the other end with either azide or 2×biotin, were generated by PCR using λ DNA template (NEB, N3011S) , . .. ∼8 ml PCR product (azide-DNA:2xbiotin-DNA = 1:1) was purified using HiSpeed Plasmid Maxi kit (Qiagen) in 1 ml NaHCO 3 (pH 8.3).

    Modification:

    Article Title: Hidden route of protein damage through oxygen-confined photooxidation
    Article Snippet: .. The DNA constructs, modified at one end with amine group and at the other end with either azide or 2×biotin, were generated by PCR using λ DNA template (NEB, N3011S) , . .. ∼8 ml PCR product (azide-DNA:2xbiotin-DNA = 1:1) was purified using HiSpeed Plasmid Maxi kit (Qiagen) in 1 ml NaHCO 3 (pH 8.3).

    Article Title: Integrated multiomics signatures to optimize the accurate diagnosis of lung cancer
    Article Snippet: For cfDNA extraction, a VAHTS Serum/Plasma Circulating DNA Kit (Vazyme, Cat # N902-02) was used, and the cfDNA was quantified with a Qubit fluorometer (Life Technologies). cfDNA (10-30 ng) was applied for library construction: adapter ligation was facilitated via the VAHTS Universal DNA Library Prep Kit for Illumina (Vazyme, Cat # ND607) according to the protocol provided by the manufacturer, with spike-in controls added (0.01 pg of each amplicon per 10 ng of cfDNA). .. The spike-in control, including 3 distinct lambda DNA amplification products (~180 bp; one without modification and the other two with 5mC and 5hmC modifications), was prepared as follow : lambda DNA was PCR amplified by Taq DNA Polymerase (NEB) and purified by AMPure XP beads (Beckman Counlter) in nonoverlapping ~180 bp amplicons, with a cocktail of dATP/dGTP/dTTP and one of the following: dCTP, dmCTP or 10% dhmCTP (Zymo)/90% dCTP. ..

    Generated:

    Article Title: Hidden route of protein damage through oxygen-confined photooxidation
    Article Snippet: .. The DNA constructs, modified at one end with amine group and at the other end with either azide or 2×biotin, were generated by PCR using λ DNA template (NEB, N3011S) , . .. ∼8 ml PCR product (azide-DNA:2xbiotin-DNA = 1:1) was purified using HiSpeed Plasmid Maxi kit (Qiagen) in 1 ml NaHCO 3 (pH 8.3).

    Polymerase Chain Reaction:

    Article Title: Hidden route of protein damage through oxygen-confined photooxidation
    Article Snippet: .. The DNA constructs, modified at one end with amine group and at the other end with either azide or 2×biotin, were generated by PCR using λ DNA template (NEB, N3011S) , . .. ∼8 ml PCR product (azide-DNA:2xbiotin-DNA = 1:1) was purified using HiSpeed Plasmid Maxi kit (Qiagen) in 1 ml NaHCO 3 (pH 8.3).

    Article Title: Integrated multiomics signatures to optimize the accurate diagnosis of lung cancer
    Article Snippet: For cfDNA extraction, a VAHTS Serum/Plasma Circulating DNA Kit (Vazyme, Cat # N902-02) was used, and the cfDNA was quantified with a Qubit fluorometer (Life Technologies). cfDNA (10-30 ng) was applied for library construction: adapter ligation was facilitated via the VAHTS Universal DNA Library Prep Kit for Illumina (Vazyme, Cat # ND607) according to the protocol provided by the manufacturer, with spike-in controls added (0.01 pg of each amplicon per 10 ng of cfDNA). .. The spike-in control, including 3 distinct lambda DNA amplification products (~180 bp; one without modification and the other two with 5mC and 5hmC modifications), was prepared as follow : lambda DNA was PCR amplified by Taq DNA Polymerase (NEB) and purified by AMPure XP beads (Beckman Counlter) in nonoverlapping ~180 bp amplicons, with a cocktail of dATP/dGTP/dTTP and one of the following: dCTP, dmCTP or 10% dhmCTP (Zymo)/90% dCTP. ..

    Activity Assay:

    Article Title: Single-cell mapping of regulatory DNA:Protein interactions
    Article Snippet: After purification, the protein was denatured at 95 °C for 5 minutes, analyzed by SDS-PAGE gel (BIORAD, 4561085) and imaged by BIORAD ChemiDoc Touch Imaging System ( ). .. DNA substrates, including lambda phage DNA (NEB, N3011) or 5’ 6-FAM-labeled 30-mer dsDNA oligonucleotides (IDT, Supplementary Note ) were used for testing nb-DddA deamination activity. ..

    Methylation:

    Article Title: Distinct Control of histone H1 expression within the Histone Locus body by CRAMP1
    Article Snippet: .. The bisulfite conversion efficiency was 99.5% for all samples based on the observed methylation level of unmethylated λ-bacteriophage DNA (New England Biolabs) spiked in to each sample. .. Sequencing was performed on an Illumina NextSeq 2000 instrument with P2 kits (75-bp single-end reads).

    other:

    Article Title: Design and in vitr o anticancer assessment of a click chemistry-derived dinuclear copper artificial metallo-nuclease
    Article Snippet: The relative extension of DNA on the cover slips was estimated by stretching lambda DNA (48,502 bp, New England Biolabs) in a similar buffer to determine 1 μm stretched DNA to be ∼3000 bp.

    Article Title: The Pre-Polarization and Concentration of Cells near Micro-Electrodes Using AC Electric Fields Enhances the Electrical Cell Lysis in a Sessile Drop
    Article Snippet: First, to find the frequency needed for the cellular DNA concentration, we separately conducted a series of experiments using commercially available lambda DNA (λ-DNA; New England Biolabs, Ipswich, MA, USA) and varied the frequency of the electric field (0–20 MHz).

    Article Title: Species-specific oxygen sensing governs the initiation of vertebrate limb regeneration
    Article Snippet: At the final step, samples were eluted in 25 μl of elution buffer (Qiagen, 19086) containing 2 pg of lambda-DNA (NEB, N3011S; tagmented with Tn5 and cleaned up on columns before use) as a spike-in.

    Control:

    Article Title: Integrated multiomics signatures to optimize the accurate diagnosis of lung cancer
    Article Snippet: For cfDNA extraction, a VAHTS Serum/Plasma Circulating DNA Kit (Vazyme, Cat # N902-02) was used, and the cfDNA was quantified with a Qubit fluorometer (Life Technologies). cfDNA (10-30 ng) was applied for library construction: adapter ligation was facilitated via the VAHTS Universal DNA Library Prep Kit for Illumina (Vazyme, Cat # ND607) according to the protocol provided by the manufacturer, with spike-in controls added (0.01 pg of each amplicon per 10 ng of cfDNA). .. The spike-in control, including 3 distinct lambda DNA amplification products (~180 bp; one without modification and the other two with 5mC and 5hmC modifications), was prepared as follow : lambda DNA was PCR amplified by Taq DNA Polymerase (NEB) and purified by AMPure XP beads (Beckman Counlter) in nonoverlapping ~180 bp amplicons, with a cocktail of dATP/dGTP/dTTP and one of the following: dCTP, dmCTP or 10% dhmCTP (Zymo)/90% dCTP. ..

    Lambda DNA Preparation:

    Article Title: Integrated multiomics signatures to optimize the accurate diagnosis of lung cancer
    Article Snippet: For cfDNA extraction, a VAHTS Serum/Plasma Circulating DNA Kit (Vazyme, Cat # N902-02) was used, and the cfDNA was quantified with a Qubit fluorometer (Life Technologies). cfDNA (10-30 ng) was applied for library construction: adapter ligation was facilitated via the VAHTS Universal DNA Library Prep Kit for Illumina (Vazyme, Cat # ND607) according to the protocol provided by the manufacturer, with spike-in controls added (0.01 pg of each amplicon per 10 ng of cfDNA). .. The spike-in control, including 3 distinct lambda DNA amplification products (~180 bp; one without modification and the other two with 5mC and 5hmC modifications), was prepared as follow : lambda DNA was PCR amplified by Taq DNA Polymerase (NEB) and purified by AMPure XP beads (Beckman Counlter) in nonoverlapping ~180 bp amplicons, with a cocktail of dATP/dGTP/dTTP and one of the following: dCTP, dmCTP or 10% dhmCTP (Zymo)/90% dCTP. ..

    Article Title: Primer-independent DNA polymerases and their use for DNA synthesis
    Article Snippet: .. Lambda DNA ladder used as a size marker was labeled by filling-in with Klenow fragment (New England Biolabs) in the presence of [α-32P]dATP. .. Replication of homopolymeric single stranded template was performed in similar conditions, using the 33-mer oligonucleotides (IDT) with the indicated sequences (Table 2), containing a terminal 3′-3′ inverted 3′-3′ inverted link to prevent both primer extension and exonucleolytic degradation.

    Amplification:

    Article Title: Integrated multiomics signatures to optimize the accurate diagnosis of lung cancer
    Article Snippet: For cfDNA extraction, a VAHTS Serum/Plasma Circulating DNA Kit (Vazyme, Cat # N902-02) was used, and the cfDNA was quantified with a Qubit fluorometer (Life Technologies). cfDNA (10-30 ng) was applied for library construction: adapter ligation was facilitated via the VAHTS Universal DNA Library Prep Kit for Illumina (Vazyme, Cat # ND607) according to the protocol provided by the manufacturer, with spike-in controls added (0.01 pg of each amplicon per 10 ng of cfDNA). .. The spike-in control, including 3 distinct lambda DNA amplification products (~180 bp; one without modification and the other two with 5mC and 5hmC modifications), was prepared as follow : lambda DNA was PCR amplified by Taq DNA Polymerase (NEB) and purified by AMPure XP beads (Beckman Counlter) in nonoverlapping ~180 bp amplicons, with a cocktail of dATP/dGTP/dTTP and one of the following: dCTP, dmCTP or 10% dhmCTP (Zymo)/90% dCTP. ..

    Purification:

    Article Title: Integrated multiomics signatures to optimize the accurate diagnosis of lung cancer
    Article Snippet: For cfDNA extraction, a VAHTS Serum/Plasma Circulating DNA Kit (Vazyme, Cat # N902-02) was used, and the cfDNA was quantified with a Qubit fluorometer (Life Technologies). cfDNA (10-30 ng) was applied for library construction: adapter ligation was facilitated via the VAHTS Universal DNA Library Prep Kit for Illumina (Vazyme, Cat # ND607) according to the protocol provided by the manufacturer, with spike-in controls added (0.01 pg of each amplicon per 10 ng of cfDNA). .. The spike-in control, including 3 distinct lambda DNA amplification products (~180 bp; one without modification and the other two with 5mC and 5hmC modifications), was prepared as follow : lambda DNA was PCR amplified by Taq DNA Polymerase (NEB) and purified by AMPure XP beads (Beckman Counlter) in nonoverlapping ~180 bp amplicons, with a cocktail of dATP/dGTP/dTTP and one of the following: dCTP, dmCTP or 10% dhmCTP (Zymo)/90% dCTP. ..

    Marker:

    Article Title: Primer-independent DNA polymerases and their use for DNA synthesis
    Article Snippet: .. Lambda DNA ladder used as a size marker was labeled by filling-in with Klenow fragment (New England Biolabs) in the presence of [α-32P]dATP. .. Replication of homopolymeric single stranded template was performed in similar conditions, using the 33-mer oligonucleotides (IDT) with the indicated sequences (Table 2), containing a terminal 3′-3′ inverted 3′-3′ inverted link to prevent both primer extension and exonucleolytic degradation.

    Labeling:

    Article Title: Primer-independent DNA polymerases and their use for DNA synthesis
    Article Snippet: .. Lambda DNA ladder used as a size marker was labeled by filling-in with Klenow fragment (New England Biolabs) in the presence of [α-32P]dATP. .. Replication of homopolymeric single stranded template was performed in similar conditions, using the 33-mer oligonucleotides (IDT) with the indicated sequences (Table 2), containing a terminal 3′-3′ inverted 3′-3′ inverted link to prevent both primer extension and exonucleolytic degradation.



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    Image Search Results


    Dendrogram generated from PFGE analysis of A. pleuropneumoniae strains digested with the ApaI restriction enzyme, with corresponding epidemiological information and antimicrobial resistance profiles. Colored boxes adjacent to each strain identify the PFGE profiles.

    Journal: Microorganisms

    Article Title: Serotypes, MIC-Based Antimicrobial Susceptibility, and Genotypic Diversity of Actinobacillus pleuropneumoniae Isolates from Diseased Pigs in Brazil

    doi: 10.3390/microorganisms14040828

    Figure Lengend Snippet: Dendrogram generated from PFGE analysis of A. pleuropneumoniae strains digested with the ApaI restriction enzyme, with corresponding epidemiological information and antimicrobial resistance profiles. Colored boxes adjacent to each strain identify the PFGE profiles.

    Article Snippet: Band sizes were estimated using a lambda DNA PFGE marker (New England BioLabs, Ipswich, MA, USA).

    Techniques: Generated