spike (New England Biolabs)

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New England Biolabs spike
Spike, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1829 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lambda+dna/Lambda+DNA/pm42102803-147-7-9
Average 97 stars, based on 1829 article reviews

spike - by Bioz Stars, 2026-09

97/100 stars

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Labeling:

Article Title: Unveiling a missing component of the atypical type IV secretion system required for natural transformation of Helicobacter pylori
Article Snippet: .. When cultures reached exponential phase (18-24h), OD was normalized to 4 in 20 μL and 200 ng of λ-DNA (New England Biolabs), labelled with Cy3 using Label IT Nucleic Acid Labeling Kit (Mirus Bio) according to manufacturer instructions, were added to the reaction. ..

Polymerase Chain Reaction:

Article Title: Cell cycle dynamics regulate H3K27 and H3K9 histone modifications in Drosophila
Article Snippet: .. Libraries were prepared by supplementing the NEBNext HighFidelity 2× PCR Master Mix with 1 pg of Tn5-tagmented lambda DNA (New England Biolabs, #N3011S) as a spike-in normalizer. .. The library indexing was performed using Illumina i5 and i7 [ ] through 15 cycles (1 × 5 min at 72 °C, 1 × 30 s at 98 °C, 13 × 10 s at 98 °C, 30 s at 63 °C, 1 × 1 min at 72 °C, hold at 4 °C).

Article Title: Cell cycle dynamics regulate H3K27 and H3K9 histone modifications in Drosophila.
Article Snippet: .. Libraries were prepared by supplementing the NEBNext HighFidelity 2× PCR Master Mix with 1 pg of Tn5-tagmented lambda DNA (New England Biolabs, #N3011S) as a spike-in normalizer. .. The library indexing was performed using Illumina i5 and i7 [106] through 15 cycles (1 × 5 min at 72 °C, 1 × 30 s at 98 °C, 13 × 10 s at 98 °C, 30 s at 63 °C, 1 × 1 min at 72 °C, hold at 4 °C).

Lambda DNA Preparation:

Article Title: A multiplex PCR-based sequencing method for the diagnosis and differentiation of echinococcosis using plasma samples, a proof-of-concept study.
Article Snippet: .. The panel used Lambda DNA as a spike-in control (NEB, N3011S) with 1,000 copies/reaction (5 primer pairs), and human genomic DNA from cell line (GM12878) as an internal quality control (1 ng/reaction) (7 primer pairs). .. Amplicon library preparation and sequencing E. granulosus and E. multilocularis gDNA were gradient-diluted to a range from 0.1 ng to 0.1 fg using nuclease-free water (Invitrogen, cat #AM9930) in a 10-fold gradient.

Translocation Assay:

Article Title: Nanopore functionality control
Article Snippet: Voltages were set and currents recorded by custom written LabView software4,20 or with ClampEx (Molecular Devices, USA). .. For translocation experiments we used linearized λ-DNA (New England Biolabs, USA) diluted in the measurement buffer prior to all experiments. ..

other:

Article Title: Recognition and remodelling of nucleosomes and hexasomes by the human INO80 complex
Article Snippet: Reactions were stopped by adding Lambda DNA (0.2 mg/ml; NEB).

Article Title: RPA directly stimulates Mer3 helicase processivity to ensure normal crossover formation in meiosis
Article Snippet: The dsDNA hairpin was obtained by PCR amplification with Phusion High-Fidelity DNA Polymerase (Thermo Scientific) using Lambda DNA (New England Biolabs) as template and oligonucleotides that include different BsaI restriction sites in each side of the PCR fragment (see Supplementary Tables and for oligonucleotides) followed by purification (QIAGEN) as described previously .

Control:

Force Spectroscopy:

Article Title: Conjugated Polymeric Nanostructures as Potential Tools in DNA Nanotechnology: Interactions with the Double Helix
Article Snippet: .. The samples prepared for the single-molecule force spectroscopy assays consist of biotin-labeled λ-DNA (48,502 base pairs, New England Biolabs) attached at the ends to a streptavidin-coated polystyrene bead with a 3 μm diameter (Bangs Laboratories) and to a streptavidin-coated glass coverslip (which serves as the bottom surface of the sample chamber used). ..

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