Review



human lambda elisa quantitation  (Bethyl)


Bioz Verified Symbol Bethyl is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93

    Structured Review

    Bethyl human lambda elisa quantitation
    Human Lambda Elisa Quantitation, supplied by Bethyl, used in various techniques. Bioz Stars score: 93/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lambda+129/Human+Lambda+Free+Light+Chain/pmc09519052-280-22-21
    Average 93 stars, based on 36 article reviews
    human lambda elisa quantitation - by Bioz Stars, 2026-09
    93/100 stars

    Images

    Related Articles

    Expressing:

    Article Title: Anti-lambda myeloma antigen (LMA) binding proteins to treat LMA-expressing cancer and autoimmune disorders
    Article Snippet: .. Similarly, human anti-LMA antibodies (6A1, 13H3 and 4A1) demonstrated high and selective affinity to human multiple myeloma cell lines expressing lambda-light chain isotypes and not to kappa-light chain expressing JJN3 cell line (FIG. 4) while clones 18E8, 7F11 and 18F9 demonstrated selective affinity to commercial lambda-light chain (Bethyl) (FIG. 2B). ..

    Clone Assay:

    Article Title: Anti-lambda myeloma antigen (LMA) binding proteins to treat LMA-expressing cancer and autoimmune disorders
    Article Snippet: .. Similarly, human anti-LMA antibodies (6A1, 13H3 and 4A1) demonstrated high and selective affinity to human multiple myeloma cell lines expressing lambda-light chain isotypes and not to kappa-light chain expressing JJN3 cell line (FIG. 4) while clones 18E8, 7F11 and 18F9 demonstrated selective affinity to commercial lambda-light chain (Bethyl) (FIG. 2B). ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Heterohybridomas producing human immunoglobulin light chains using CD138-selected bone marrow cells.
    Article Snippet: Background: Light chain research is hampered by lack of mammalian cell lines producing human light chains (FLC).. Therefore, we used heterohybridoma (HH) technology to produce clones making FLC thereby providing tools to study light chain behavior.. Methods: Marrow CD138+ cells from patients with multiple myeloma (MM) and polyclonal gammopathy (PG) were selected, fused with B5-6 T cells and cultured in hypoxanthine-aminopterin-thymidine medium (HAT).

    Article Title: Treatment of al amyloidosis with melflufen
    Article Snippet: .. Levels of kappa and lambda light chains as well as BCMA from the serum was analysed on days 9 and 16 post tumor inoculation using commercial ELISA kits (below) and manufacturer's protocols: Human Kappa ELISA (Bethyl Laboratories, #E80-115) Human Lambda ELISA (Bethyl Laboratories, #E80-116) Human BCMA ELISA (R&D Systems, #DY193). ..

    Article Title: Heterohybridomas producing human immunoglobulin light chains using CD138-selected bone marrow cells
    Article Snippet: .. Heterohybridoma supernatants for ELISA were evaluated in quantitative sandwich ELISA for human IgA, IgG, IgM, κ and λ light chains (Bethyl Laboratories, Montgomery, TX) according to the manufacturers’ instructions. ..

    Article Title: Therapeutic implications of mitochondrial stress-induced proteasome inhibitor resistance in multiple myeloma.
    Article Snippet: .. Lysates of the cell pellets were prepared in RIPA buffer, and the lambda and kappa light chain antibodies were measured using Bethyl Human Lambda ELISA Quantitation Set (E80-116) and Bethyl Human Kappa ELISA Quantitation Set (#E80-115) following the manufacturer’s protocol. .. Bacterial clones containing ATF4 short hairpin RNAs were obtained from Sigma-Aldrich (TRCN0000013573 and TRCN0000013574) and plated on LB agar plates containing ampicillin.

    Article Title: Therapeutic implications of mitochondrial stress–induced proteasome inhibitor resistance in multiple myeloma
    Article Snippet: .. Lysates of the cell pellets were prepared in RIPA buffer, and the lambda and kappa light chain antibodies were measured using Bethyl Human Lambda ELISA Quantitation Set (E80-116) and Bethyl Human Kappa ELISA Quantitation Set (#E80-115) following the manufacturer’s protocol. .. Bacterial clones containing ATF4 short hairpin RNAs were obtained from Sigma-Aldrich (TRCN0000013573 and TRCN0000013574) and plated on LB agar plates containing ampicillin.

    Article Title: A non-ligand surrogate agonist antibody that enhances canonical Wnt signaling and bone regeneration
    Article Snippet: .. One week post treatment, mice were anesthetized and scanned by micro-CT. One week post CT scanning, blood and femurs were collected from the mice, and free human Ig-lambda light chains in serum was assessed using a Human Lambda ELISA Kit (Bethyl Laboratories) according to manufacturer’s instructions. ..

    Sandwich ELISA:

    Article Title: Heterohybridomas producing human immunoglobulin light chains using CD138-selected bone marrow cells.
    Article Snippet: Background: Light chain research is hampered by lack of mammalian cell lines producing human light chains (FLC).. Therefore, we used heterohybridoma (HH) technology to produce clones making FLC thereby providing tools to study light chain behavior.. Methods: Marrow CD138+ cells from patients with multiple myeloma (MM) and polyclonal gammopathy (PG) were selected, fused with B5-6 T cells and cultured in hypoxanthine-aminopterin-thymidine medium (HAT).

    Article Title: Heterohybridomas producing human immunoglobulin light chains using CD138-selected bone marrow cells
    Article Snippet: .. Heterohybridoma supernatants for ELISA were evaluated in quantitative sandwich ELISA for human IgA, IgG, IgM, κ and λ light chains (Bethyl Laboratories, Montgomery, TX) according to the manufacturers’ instructions. ..

    Quantitation Assay:

    Article Title: Therapeutic implications of mitochondrial stress-induced proteasome inhibitor resistance in multiple myeloma.
    Article Snippet: .. Lysates of the cell pellets were prepared in RIPA buffer, and the lambda and kappa light chain antibodies were measured using Bethyl Human Lambda ELISA Quantitation Set (E80-116) and Bethyl Human Kappa ELISA Quantitation Set (#E80-115) following the manufacturer’s protocol. .. Bacterial clones containing ATF4 short hairpin RNAs were obtained from Sigma-Aldrich (TRCN0000013573 and TRCN0000013574) and plated on LB agar plates containing ampicillin.

    Article Title: Therapeutic implications of mitochondrial stress–induced proteasome inhibitor resistance in multiple myeloma
    Article Snippet: .. Lysates of the cell pellets were prepared in RIPA buffer, and the lambda and kappa light chain antibodies were measured using Bethyl Human Lambda ELISA Quantitation Set (E80-116) and Bethyl Human Kappa ELISA Quantitation Set (#E80-115) following the manufacturer’s protocol. .. Bacterial clones containing ATF4 short hairpin RNAs were obtained from Sigma-Aldrich (TRCN0000013573 and TRCN0000013574) and plated on LB agar plates containing ampicillin.

    Micro-CT:

    Article Title: A non-ligand surrogate agonist antibody that enhances canonical Wnt signaling and bone regeneration
    Article Snippet: .. One week post treatment, mice were anesthetized and scanned by micro-CT. One week post CT scanning, blood and femurs were collected from the mice, and free human Ig-lambda light chains in serum was assessed using a Human Lambda ELISA Kit (Bethyl Laboratories) according to manufacturer’s instructions. ..



    Similar Products

    90
    Agilent technologies lambda mouse 129-sv/j genomic dna library
    Lambda Mouse 129 Sv/J Genomic Dna Library, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lambda+129/pmc03990712-29-18-24
    Average 90 stars, based on 1 article reviews
    lambda mouse 129-sv/j genomic dna library - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    90
    Agilent technologies lime phage clone obtained by screening a lambda library made from the 129 sv mouse strain
    Lime Phage Clone Obtained By Screening A Lambda Library Made From The 129 Sv Mouse Strain, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lambda+129/pmc07700840__molce___43___921_Supple-0-33-37
    Average 90 stars, based on 1 article reviews
    lime phage clone obtained by screening a lambda library made from the 129 sv mouse strain - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    96
    Bethyl human lambda elisa quantitation
    Human Lambda Elisa Quantitation, supplied by Bethyl, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lambda+129/Human+Albumin+ELISA+Quantitation+Set/pmc05400723-650-46-51
    Average 96 stars, based on 1 article reviews
    human lambda elisa quantitation - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    96
    Bethyl human lambda elisa quantitation set
    Human multiple myeloma cell line U266-ffluc was i.v. (tail vein) injected into NSG mice. After 4 weeks, the mice were randomized into 3 groups comprising 8–9 mice each and treated with i.v. (tail vein) injections of 3 mg/kg of the ADC, the unconjugated antibody, and vehicle alone. (A) Human <t>lambda</t> light chain concentrations correlating with tumor burden were determined by <t>ELISA</t> at the indicated time points and plotted as mean ± SD values. The p value (t-test) compares the ADC group to the unconjugated antibody group. (B) Kaplan-Meier survival curve. The p value (log-rank test) compares the ADC group to the unconjugated antibody group. See also Figure S3 and Table S3.
    Human Lambda Elisa Quantitation Set, supplied by Bethyl, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lambda+129/Human+Albumin+ELISA+Quantitation+Set/pmc05400723-52-0-6
    Average 96 stars, based on 1 article reviews
    human lambda elisa quantitation set - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    90
    Agilent technologies lambda fixii 129/sv phage library
    Human multiple myeloma cell line U266-ffluc was i.v. (tail vein) injected into NSG mice. After 4 weeks, the mice were randomized into 3 groups comprising 8–9 mice each and treated with i.v. (tail vein) injections of 3 mg/kg of the ADC, the unconjugated antibody, and vehicle alone. (A) Human <t>lambda</t> light chain concentrations correlating with tumor burden were determined by <t>ELISA</t> at the indicated time points and plotted as mean ± SD values. The p value (t-test) compares the ADC group to the unconjugated antibody group. (B) Kaplan-Meier survival curve. The p value (log-rank test) compares the ADC group to the unconjugated antibody group. See also Figure S3 and Table S3.
    Lambda Fixii 129/Sv Phage Library, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lambda+129/pm25027639-41-17-20
    Average 90 stars, based on 1 article reviews
    lambda fixii 129/sv phage library - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    90
    Agilent technologies lambda fix ii mouse 129/sv genomic library
    Human multiple myeloma cell line U266-ffluc was i.v. (tail vein) injected into NSG mice. After 4 weeks, the mice were randomized into 3 groups comprising 8–9 mice each and treated with i.v. (tail vein) injections of 3 mg/kg of the ADC, the unconjugated antibody, and vehicle alone. (A) Human <t>lambda</t> light chain concentrations correlating with tumor burden were determined by <t>ELISA</t> at the indicated time points and plotted as mean ± SD values. The p value (t-test) compares the ADC group to the unconjugated antibody group. (B) Kaplan-Meier survival curve. The p value (log-rank test) compares the ADC group to the unconjugated antibody group. See also Figure S3 and Table S3.
    Lambda Fix Ii Mouse 129/Sv Genomic Library, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lambda+129/pmc04156920-381-16-19
    Average 90 stars, based on 1 article reviews
    lambda fix ii mouse 129/sv genomic library - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    90
    Agilent technologies the lambda mouse 129-sv/j genomic dna library
    Human multiple myeloma cell line U266-ffluc was i.v. (tail vein) injected into NSG mice. After 4 weeks, the mice were randomized into 3 groups comprising 8–9 mice each and treated with i.v. (tail vein) injections of 3 mg/kg of the ADC, the unconjugated antibody, and vehicle alone. (A) Human <t>lambda</t> light chain concentrations correlating with tumor burden were determined by <t>ELISA</t> at the indicated time points and plotted as mean ± SD values. The p value (t-test) compares the ADC group to the unconjugated antibody group. (B) Kaplan-Meier survival curve. The p value (log-rank test) compares the ADC group to the unconjugated antibody group. See also Figure S3 and Table S3.
    The Lambda Mouse 129 Sv/J Genomic Dna Library, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lambda+129/10__1074_slash_jbc__m115__650531-61-29-35
    Average 90 stars, based on 1 article reviews
    the lambda mouse 129-sv/j genomic dna library - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    90
    Agilent technologies mouse 129/svevtachbr lambda genomic library
    Human multiple myeloma cell line U266-ffluc was i.v. (tail vein) injected into NSG mice. After 4 weeks, the mice were randomized into 3 groups comprising 8–9 mice each and treated with i.v. (tail vein) injections of 3 mg/kg of the ADC, the unconjugated antibody, and vehicle alone. (A) Human <t>lambda</t> light chain concentrations correlating with tumor burden were determined by <t>ELISA</t> at the indicated time points and plotted as mean ± SD values. The p value (t-test) compares the ADC group to the unconjugated antibody group. (B) Kaplan-Meier survival curve. The p value (log-rank test) compares the ADC group to the unconjugated antibody group. See also Figure S3 and Table S3.
    Mouse 129/Svevtachbr Lambda Genomic Library, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lambda+129/pm25131300-38-16-20
    Average 90 stars, based on 1 article reviews
    mouse 129/svevtachbr lambda genomic library - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    90
    Agilent technologies lambda fixii mouse 129/svj genomic library
    Human multiple myeloma cell line U266-ffluc was i.v. (tail vein) injected into NSG mice. After 4 weeks, the mice were randomized into 3 groups comprising 8–9 mice each and treated with i.v. (tail vein) injections of 3 mg/kg of the ADC, the unconjugated antibody, and vehicle alone. (A) Human <t>lambda</t> light chain concentrations correlating with tumor burden were determined by <t>ELISA</t> at the indicated time points and plotted as mean ± SD values. The p value (t-test) compares the ADC group to the unconjugated antibody group. (B) Kaplan-Meier survival curve. The p value (log-rank test) compares the ADC group to the unconjugated antibody group. See also Figure S3 and Table S3.
    Lambda Fixii Mouse 129/Svj Genomic Library, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lambda+129/pm20162674-71-8-14
    Average 90 stars, based on 1 article reviews
    lambda fixii mouse 129/svj genomic library - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    90
    Agilent technologies lambda fix 11 129 mouse liver genomic library
    Human multiple myeloma cell line U266-ffluc was i.v. (tail vein) injected into NSG mice. After 4 weeks, the mice were randomized into 3 groups comprising 8–9 mice each and treated with i.v. (tail vein) injections of 3 mg/kg of the ADC, the unconjugated antibody, and vehicle alone. (A) Human <t>lambda</t> light chain concentrations correlating with tumor burden were determined by <t>ELISA</t> at the indicated time points and plotted as mean ± SD values. The p value (t-test) compares the ADC group to the unconjugated antibody group. (B) Kaplan-Meier survival curve. The p value (log-rank test) compares the ADC group to the unconjugated antibody group. See also Figure S3 and Table S3.
    Lambda Fix 11 129 Mouse Liver Genomic Library, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lambda+129/us07534859-351-7-15
    Average 90 stars, based on 1 article reviews
    lambda fix 11 129 mouse liver genomic library - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    Image Search Results


    Human multiple myeloma cell line U266-ffluc was i.v. (tail vein) injected into NSG mice. After 4 weeks, the mice were randomized into 3 groups comprising 8–9 mice each and treated with i.v. (tail vein) injections of 3 mg/kg of the ADC, the unconjugated antibody, and vehicle alone. (A) Human lambda light chain concentrations correlating with tumor burden were determined by ELISA at the indicated time points and plotted as mean ± SD values. The p value (t-test) compares the ADC group to the unconjugated antibody group. (B) Kaplan-Meier survival curve. The p value (log-rank test) compares the ADC group to the unconjugated antibody group. See also Figure S3 and Table S3.

    Journal: Cell chemical biology

    Article Title: Stable and potent selenomab-drug conjugates

    doi: 10.1016/j.chembiol.2017.02.012

    Figure Lengend Snippet: Human multiple myeloma cell line U266-ffluc was i.v. (tail vein) injected into NSG mice. After 4 weeks, the mice were randomized into 3 groups comprising 8–9 mice each and treated with i.v. (tail vein) injections of 3 mg/kg of the ADC, the unconjugated antibody, and vehicle alone. (A) Human lambda light chain concentrations correlating with tumor burden were determined by ELISA at the indicated time points and plotted as mean ± SD values. The p value (t-test) compares the ADC group to the unconjugated antibody group. (B) Kaplan-Meier survival curve. The p value (log-rank test) compares the ADC group to the unconjugated antibody group. See also Figure S3 and Table S3.

    Article Snippet: Human Lambda ELISA Quantitation Set , Bethyl Laboratories , Cat#E80-116.

    Techniques: Injection, Enzyme-linked Immunosorbent Assay

    (A) Human breast cancer cell line KPL-4 was xenografted into the mammary fat pads of female NSG mice, grown to ~150 mm3, randomized into 5 groups comprising 6 mice each, and treated with i.v. (tail vein) injections of the indicated ADCs and controls four times every week at 5 mg/kg. Mean ± SD values are plotted. (B) Four female CD-1 mice were injected i.v. with 6 mg/kg of anti-HER2 scFv-Fc(Ser396Sec)/CN29. The plasma concentrations of total antibody and intact ADC were quantified by ELISA at the indicated time points, using HER2 ECD for capturing and either anti-His mAb (total antibody) or anti-MMAF mAb (intact ADC) for detection. Shown are mean ± SD values for each time point. See also Figure S5, Figure S6, Figure S7, and Table S6.

    Journal: Cell chemical biology

    Article Title: Stable and potent selenomab-drug conjugates

    doi: 10.1016/j.chembiol.2017.02.012

    Figure Lengend Snippet: (A) Human breast cancer cell line KPL-4 was xenografted into the mammary fat pads of female NSG mice, grown to ~150 mm3, randomized into 5 groups comprising 6 mice each, and treated with i.v. (tail vein) injections of the indicated ADCs and controls four times every week at 5 mg/kg. Mean ± SD values are plotted. (B) Four female CD-1 mice were injected i.v. with 6 mg/kg of anti-HER2 scFv-Fc(Ser396Sec)/CN29. The plasma concentrations of total antibody and intact ADC were quantified by ELISA at the indicated time points, using HER2 ECD for capturing and either anti-His mAb (total antibody) or anti-MMAF mAb (intact ADC) for detection. Shown are mean ± SD values for each time point. See also Figure S5, Figure S6, Figure S7, and Table S6.

    Article Snippet: Human Lambda ELISA Quantitation Set , Bethyl Laboratories , Cat#E80-116.

    Techniques: Injection, Clinical Proteomics, Enzyme-linked Immunosorbent Assay

    KEY RESOURCES TABLE

    Journal: Cell chemical biology

    Article Title: Stable and potent selenomab-drug conjugates

    doi: 10.1016/j.chembiol.2017.02.012

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: Human Lambda ELISA Quantitation Set , Bethyl Laboratories , Cat#E80-116.

    Techniques: Virus, Recombinant, Marker, Conjugation Assay, Proliferation Assay, Enzyme-linked Immunosorbent Assay, Quantitation Assay, Software