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filter wheel sutter instruments lambda 10b  (Sutter Instrument Company)


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    Structured Review

    Sutter Instrument Company filter wheel sutter instruments lambda 10b

    Filter Wheel Sutter Instruments Lambda 10b, supplied by Sutter Instrument Company, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lambda+10b/filter+wheel+sutter+lambda+10+c/pmc08651295-176-8-5
    Average 90 stars, based on 1 article reviews
    filter wheel sutter instruments lambda 10b - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Lactate is an energy substrate for rodent cortical neurons and enhances their firing activity"

    Article Title: Lactate is an energy substrate for rodent cortical neurons and enhances their firing activity

    Journal: eLife

    doi: 10.7554/eLife.71424


    Figure Legend Snippet:

    Techniques Used: Recombinant, Plasmid Preparation, Sequencing, Reverse Transcription, Software, Microscopy

    Related Articles

    Fluorescence:

    Article Title: Patterning and growth are coordinated early in the cell cycle
    Article Snippet: .. Fluorescence emitted from the sample was detected through a water-immersion detection objective (Olympus XLUMPLFLN20XW, 20X, NA = 1.0), filtered through detection filters specific to the appropriate fluorophore (Semrock, GFP: FF03-525/50-25; mKATE2: FF01-624/40-25; CFP: FF01-482/35-25) mounted on a filter wheel (Sutter Instruments Lambda 10-3) and a short-pass 750nm filter, and projected onto a cMOS camera (Andor Zyla 5.5) through a double-achromatic tube lens (focal length = 300 mm; Thorlabs ACT508-300-A-ML), to yield a final magnification of 33X. ..

    Article Title: Construction, imaging and analysis of FRET-based tension sensors in living cells
    Article Snippet: Details on microsphere selection and use can be found in Section 2.3.2 Phosphate buffered saline (PBS): 137 mM NaCl, 2.7 mM KCl, 10 mM Na 2 HPO 4 , 2 mM KH 2 PO 4 , pH to 7.2–7.4 with HCl Human fibronectin (Fisher Scientific, CB-40008A) Growth medium: DMEM, high glucose (Sigma Aldrich, D6429) supplemented with 1X non-essential amino acids (Life Technologies, 11140), 10% FBS (HyClone), 1X Antibiotic Antimycotic Solution (Sigma Aldrich, A5955) Transfection reagents: Plasmid DNA constructs, Lipofectamine 2000 (Life Technologies, 11668), OptiMEM I (Life Technologies, 11058) Imaging medium: Medium 199, without phenol red, which contains sodium bicarbonate for pH buffering (Life Technologies, 11043) supplemented with 10% FBS (HyClone) Immersion Oil (Cargille, Type LDF) .. Inverted fluorescence microscope (Olympus, IX83) Xenon arc lamp (Sutter Instruments, Lambda LS) Objective (Olympus, UPlanSApo-60X, 1.35NA oil immersion) Motorized filter wheels (Sutter Instruments, Lambda 10-3) Automated Stage (Prior, H117EIX3) Vibration damping laboratory table (Ametek, Technical Manufacturing Corporation, 63-500 Series) Digital camera (Hamamatsu, ORCA-Flash4.0 V2, {"type":"entrez-nucleotide","attrs":{"text":"C11440","term_id":"1536511","term_text":"C11440"}} C11440 ) Excitation and emission filters and dichroic mirror (depend on donor and acceptor fluorophores) Specimen warming system and objective heater (Bioptechs, 403-1926 and 150803) Image acquisition software (Metamorph Advanced, Olympus) Marker or diamond tip pen ..

    Imaging:

    Article Title: Study of the Actin Cytoskeleton in Live Endothelial Cells Expressing GFP-Actin
    Article Snippet: .. Our system is a Nikon Eclipse TE-2000U with the following components: Sutter Instruments Lambda LS 300 W xenon lamp Sutter Instruments Lambda 10-3 excitation filter wheel with SmartShutter and S492 filter (D350 and S572 exciter filters are also available for UV and RFP applications) Dichroic 2002bs emitter (Nikon 61002m) CI Plan Fluor 10X DLL Objective, NA 0.30 (Nikon MRH10100) Plan Fluor ELWD 40X DM Objective, NA 0.60 (Nikon MRH08420) Plan Apo VC 100X Oil Objective, NA 1.40 (Nikon MRD01901) Photometrics CoolSNAP HQ2 camera, 1392 x 1040 imaging array, 6.45 x 6.45 μm pixels(Roper Scientific) We also have two software packages that can be used for image acquisition. .. Nikon Elements-AR 3.0, and Metamorph 6.1.

    Software:

    Article Title: Study of the Actin Cytoskeleton in Live Endothelial Cells Expressing GFP-Actin
    Article Snippet: .. Our system is a Nikon Eclipse TE-2000U with the following components: Sutter Instruments Lambda LS 300 W xenon lamp Sutter Instruments Lambda 10-3 excitation filter wheel with SmartShutter and S492 filter (D350 and S572 exciter filters are also available for UV and RFP applications) Dichroic 2002bs emitter (Nikon 61002m) CI Plan Fluor 10X DLL Objective, NA 0.30 (Nikon MRH10100) Plan Fluor ELWD 40X DM Objective, NA 0.60 (Nikon MRH08420) Plan Apo VC 100X Oil Objective, NA 1.40 (Nikon MRD01901) Photometrics CoolSNAP HQ2 camera, 1392 x 1040 imaging array, 6.45 x 6.45 μm pixels(Roper Scientific) We also have two software packages that can be used for image acquisition. .. Nikon Elements-AR 3.0, and Metamorph 6.1.

    Article Title: Construction, imaging and analysis of FRET-based tension sensors in living cells
    Article Snippet: Details on microsphere selection and use can be found in Section 2.3.2 Phosphate buffered saline (PBS): 137 mM NaCl, 2.7 mM KCl, 10 mM Na 2 HPO 4 , 2 mM KH 2 PO 4 , pH to 7.2–7.4 with HCl Human fibronectin (Fisher Scientific, CB-40008A) Growth medium: DMEM, high glucose (Sigma Aldrich, D6429) supplemented with 1X non-essential amino acids (Life Technologies, 11140), 10% FBS (HyClone), 1X Antibiotic Antimycotic Solution (Sigma Aldrich, A5955) Transfection reagents: Plasmid DNA constructs, Lipofectamine 2000 (Life Technologies, 11668), OptiMEM I (Life Technologies, 11058) Imaging medium: Medium 199, without phenol red, which contains sodium bicarbonate for pH buffering (Life Technologies, 11043) supplemented with 10% FBS (HyClone) Immersion Oil (Cargille, Type LDF) .. Inverted fluorescence microscope (Olympus, IX83) Xenon arc lamp (Sutter Instruments, Lambda LS) Objective (Olympus, UPlanSApo-60X, 1.35NA oil immersion) Motorized filter wheels (Sutter Instruments, Lambda 10-3) Automated Stage (Prior, H117EIX3) Vibration damping laboratory table (Ametek, Technical Manufacturing Corporation, 63-500 Series) Digital camera (Hamamatsu, ORCA-Flash4.0 V2, {"type":"entrez-nucleotide","attrs":{"text":"C11440","term_id":"1536511","term_text":"C11440"}} C11440 ) Excitation and emission filters and dichroic mirror (depend on donor and acceptor fluorophores) Specimen warming system and objective heater (Bioptechs, 403-1926 and 150803) Image acquisition software (Metamorph Advanced, Olympus) Marker or diamond tip pen ..

    Microscopy:

    Article Title: Construction, imaging and analysis of FRET-based tension sensors in living cells
    Article Snippet: Details on microsphere selection and use can be found in Section 2.3.2 Phosphate buffered saline (PBS): 137 mM NaCl, 2.7 mM KCl, 10 mM Na 2 HPO 4 , 2 mM KH 2 PO 4 , pH to 7.2–7.4 with HCl Human fibronectin (Fisher Scientific, CB-40008A) Growth medium: DMEM, high glucose (Sigma Aldrich, D6429) supplemented with 1X non-essential amino acids (Life Technologies, 11140), 10% FBS (HyClone), 1X Antibiotic Antimycotic Solution (Sigma Aldrich, A5955) Transfection reagents: Plasmid DNA constructs, Lipofectamine 2000 (Life Technologies, 11668), OptiMEM I (Life Technologies, 11058) Imaging medium: Medium 199, without phenol red, which contains sodium bicarbonate for pH buffering (Life Technologies, 11043) supplemented with 10% FBS (HyClone) Immersion Oil (Cargille, Type LDF) .. Inverted fluorescence microscope (Olympus, IX83) Xenon arc lamp (Sutter Instruments, Lambda LS) Objective (Olympus, UPlanSApo-60X, 1.35NA oil immersion) Motorized filter wheels (Sutter Instruments, Lambda 10-3) Automated Stage (Prior, H117EIX3) Vibration damping laboratory table (Ametek, Technical Manufacturing Corporation, 63-500 Series) Digital camera (Hamamatsu, ORCA-Flash4.0 V2, {"type":"entrez-nucleotide","attrs":{"text":"C11440","term_id":"1536511","term_text":"C11440"}} C11440 ) Excitation and emission filters and dichroic mirror (depend on donor and acceptor fluorophores) Specimen warming system and objective heater (Bioptechs, 403-1926 and 150803) Image acquisition software (Metamorph Advanced, Olympus) Marker or diamond tip pen ..

    Article Title: Concurrent Viewing of H&E and Multiplex Immunohistochemistry in Clinical Specimens.
    Article Snippet: .. A 100 W tungsten-halogen microscope lamp (Olympus model U-LH100) was the source for broad-spectrum light, and the means to select different bands of light was either a Sutter Lambda 10-3 10-position filter wheel (Sutter Instruments, Novato, CA, USA) placed between the microscope lamp and the microscope illumination port, or a Thorlabs CFS1 filter slider placed between the microscope camera port and color camera. .. Diagnostics 2025, 15, x FOR PEER REVIEW 4 of 18 Kiralux, 5.0 Mpixel color camera (model CS505CU; Thorlabs, Newton, NJ, USA), sources of visible white light and invisible light, and a means to select different wavelengths of light.

    Marker:

    Article Title: Construction, imaging and analysis of FRET-based tension sensors in living cells
    Article Snippet: Details on microsphere selection and use can be found in Section 2.3.2 Phosphate buffered saline (PBS): 137 mM NaCl, 2.7 mM KCl, 10 mM Na 2 HPO 4 , 2 mM KH 2 PO 4 , pH to 7.2–7.4 with HCl Human fibronectin (Fisher Scientific, CB-40008A) Growth medium: DMEM, high glucose (Sigma Aldrich, D6429) supplemented with 1X non-essential amino acids (Life Technologies, 11140), 10% FBS (HyClone), 1X Antibiotic Antimycotic Solution (Sigma Aldrich, A5955) Transfection reagents: Plasmid DNA constructs, Lipofectamine 2000 (Life Technologies, 11668), OptiMEM I (Life Technologies, 11058) Imaging medium: Medium 199, without phenol red, which contains sodium bicarbonate for pH buffering (Life Technologies, 11043) supplemented with 10% FBS (HyClone) Immersion Oil (Cargille, Type LDF) .. Inverted fluorescence microscope (Olympus, IX83) Xenon arc lamp (Sutter Instruments, Lambda LS) Objective (Olympus, UPlanSApo-60X, 1.35NA oil immersion) Motorized filter wheels (Sutter Instruments, Lambda 10-3) Automated Stage (Prior, H117EIX3) Vibration damping laboratory table (Ametek, Technical Manufacturing Corporation, 63-500 Series) Digital camera (Hamamatsu, ORCA-Flash4.0 V2, {"type":"entrez-nucleotide","attrs":{"text":"C11440","term_id":"1536511","term_text":"C11440"}} C11440 ) Excitation and emission filters and dichroic mirror (depend on donor and acceptor fluorophores) Specimen warming system and objective heater (Bioptechs, 403-1926 and 150803) Image acquisition software (Metamorph Advanced, Olympus) Marker or diamond tip pen ..

    other:

    Article Title: Lactate is an energy substrate for rodent cortical neurons and enhances their firing activity
    Article Snippet: Other , Filter wheel , Sutter Instruments , Lambda 10B , .

    Article Title: A Method to Simultaneously Detect Changes in Intracellular Ca2+ Concentration and Cell Volume
    Article Snippet: ⎯A method to simultaneously assess the changes in intracellular calcium concentration and cell volume in single cells was developed using the Ca2+-sensitive f luorescent probe Fura-2 and a three-dimensional image-surface reconstruction technique, respectively.. Studies with this method showed that Fura-2 loading had no significant effect on the kinetics of A549 human epithelial cell swelling in a hypotonic solution, as well as the volume restoration kinetics.. Significant changes in intracellular Ca2+ concentration were not observed in the examined volume modulation range.



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    Image Search Results


    Journal: eLife

    Article Title: Lactate is an energy substrate for rodent cortical neurons and enhances their firing activity

    doi: 10.7554/eLife.71424

    Figure Lengend Snippet:

    Article Snippet: Other , Filter wheel , Sutter Instruments , Lambda 10B , .

    Techniques: Recombinant, Plasmid Preparation, Sequencing, Reverse Transcription, Software, Microscopy