Review




Structured Review

ActivX Inc plug-in
Plug In, supplied by ActivX Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/java+or+activex+software+components/plug+in/us07540011-178-13-19
Average 90 stars, based on 1 article reviews
plug-in - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Software:

Article Title: Method and client for using an embedded ActiveX plug-in in a browser
Article Snippet: Therefore, it may be checked and judged whether the plug-in has already been installed in the computer system utilizing the correspondence between the class identifier and the ActiveX plug-in.

Article Title: Separate plug-in processes in browsers and applications thereof
Article Snippet: In this way, a plug-in instance is developed to understand one standard (such as NPAPI) may be able to access plug-ins implementing another standard (such as ActiveX).

Article Title: One-time dynamic positioning authentication method and system and password alteration method
Article Snippet: When displaying the full-element dynamic factor table, the client does not need to load or install any additional plug-in, e.g. ActiveX.

Article Title: Distributed system for monitoring patient video, audio and medical parameter data
Article Snippet: In response to user selection of a particular patient in a clinical application display image provided on portable processing device 12 by user interface 26, a parameter is acquired from a medical record of a patient and provided in a URL data field to a Web-enabled plug-in (such as a Plug-in or other executable procedure, such as an ActiveX component) in server 20 (or in another embodiment in portable processing device 12), for example.

Article Title: Automatic selection of user-oriented web content
Article Snippet: The tool can take the form of a toolbar, plug-in, extension, or add-on to be installed in a browser, an ActiveX control, a stand-alone application, etc.

Article Title: Detection of malicious script operations using statistical analysis
Article Snippet: Generally, the security server 102 generates security data that describe whether a sequence of script operations performed by a script object(s) on a web page, or whether script operations performed by a browser add-on or extension object (e.g., an ActiveX control or plug-in), will cause a browser security vulnerability.

Article Title: System and method for sending, delivery and receiving of digital content
Article Snippet: In another embodiment, the sending connector 204 may be part of a web page (e.g., an applet, plug-in, ActiveX program, etc.) that is executing on a browser, where the browser may be rendering a web page that serves as transmission initiator 206.

Article Title: Method and system of facilitating automatic login to a web site using an Internet browser
Article Snippet: When an Internet user launches or activates a browser, the library file is opened and the ActiveX control or Plug-in code contained within that file is made available to the browser and incorporated into the browser interface, thus causing the interface object to be displayed with the browser interface.

Stable Transfection:

Article Title: Method and client for using an embedded ActiveX plug-in in a browser
Article Snippet: Therefore, it may be checked and judged whether the plug-in has already been installed in the computer system utilizing the correspondence between the class identifier and the ActiveX plug-in.

Article Title: Separate plug-in processes in browsers and applications thereof
Article Snippet: In this way, a plug-in instance is developed to understand one standard (such as NPAPI) may be able to access plug-ins implementing another standard (such as ActiveX).

Article Title: One-time dynamic positioning authentication method and system and password alteration method
Article Snippet: When displaying the full-element dynamic factor table, the client does not need to load or install any additional plug-in, e.g. ActiveX.

Article Title: Distributed system for monitoring patient video, audio and medical parameter data
Article Snippet: In response to user selection of a particular patient in a clinical application display image provided on portable processing device 12 by user interface 26, a parameter is acquired from a medical record of a patient and provided in a URL data field to a Web-enabled plug-in (such as a Plug-in or other executable procedure, such as an ActiveX component) in server 20 (or in another embodiment in portable processing device 12), for example.

Article Title: Automatic selection of user-oriented web content
Article Snippet: The tool can take the form of a toolbar, plug-in, extension, or add-on to be installed in a browser, an ActiveX control, a stand-alone application, etc.

Article Title: Detection of malicious script operations using statistical analysis
Article Snippet: Generally, the security server 102 generates security data that describe whether a sequence of script operations performed by a script object(s) on a web page, or whether script operations performed by a browser add-on or extension object (e.g., an ActiveX control or plug-in), will cause a browser security vulnerability.

Article Title: System and method for sending, delivery and receiving of digital content
Article Snippet: In another embodiment, the sending connector 204 may be part of a web page (e.g., an applet, plug-in, ActiveX program, etc.) that is executing on a browser, where the browser may be rendering a web page that serves as transmission initiator 206.

Article Title: Method and system of facilitating automatic login to a web site using an Internet browser
Article Snippet: When an Internet user launches or activates a browser, the library file is opened and the ActiveX control or Plug-in code contained within that file is made available to the browser and incorporated into the browser interface, thus causing the interface object to be displayed with the browser interface.

Modification:

Article Title: Method and client for using an embedded ActiveX plug-in in a browser
Article Snippet: Therefore, it may be checked and judged whether the plug-in has already been installed in the computer system utilizing the correspondence between the class identifier and the ActiveX plug-in.

Article Title: Separate plug-in processes in browsers and applications thereof
Article Snippet: In this way, a plug-in instance is developed to understand one standard (such as NPAPI) may be able to access plug-ins implementing another standard (such as ActiveX).

Article Title: One-time dynamic positioning authentication method and system and password alteration method
Article Snippet: When displaying the full-element dynamic factor table, the client does not need to load or install any additional plug-in, e.g. ActiveX.

Article Title: Distributed system for monitoring patient video, audio and medical parameter data
Article Snippet: In response to user selection of a particular patient in a clinical application display image provided on portable processing device 12 by user interface 26, a parameter is acquired from a medical record of a patient and provided in a URL data field to a Web-enabled plug-in (such as a Plug-in or other executable procedure, such as an ActiveX component) in server 20 (or in another embodiment in portable processing device 12), for example.

Article Title: Automatic selection of user-oriented web content
Article Snippet: The tool can take the form of a toolbar, plug-in, extension, or add-on to be installed in a browser, an ActiveX control, a stand-alone application, etc.

Article Title: Detection of malicious script operations using statistical analysis
Article Snippet: Generally, the security server 102 generates security data that describe whether a sequence of script operations performed by a script object(s) on a web page, or whether script operations performed by a browser add-on or extension object (e.g., an ActiveX control or plug-in), will cause a browser security vulnerability.

Article Title: System and method for sending, delivery and receiving of digital content
Article Snippet: In another embodiment, the sending connector 204 may be part of a web page (e.g., an applet, plug-in, ActiveX program, etc.) that is executing on a browser, where the browser may be rendering a web page that serves as transmission initiator 206.

Article Title: Method and system of facilitating automatic login to a web site using an Internet browser
Article Snippet: When an Internet user launches or activates a browser, the library file is opened and the ActiveX control or Plug-in code contained within that file is made available to the browser and incorporated into the browser interface, thus causing the interface object to be displayed with the browser interface.

Generated:

Article Title: Method and client for using an embedded ActiveX plug-in in a browser
Article Snippet: Therefore, it may be checked and judged whether the plug-in has already been installed in the computer system utilizing the correspondence between the class identifier and the ActiveX plug-in.

Article Title: Separate plug-in processes in browsers and applications thereof
Article Snippet: In this way, a plug-in instance is developed to understand one standard (such as NPAPI) may be able to access plug-ins implementing another standard (such as ActiveX).

Article Title: One-time dynamic positioning authentication method and system and password alteration method
Article Snippet: When displaying the full-element dynamic factor table, the client does not need to load or install any additional plug-in, e.g. ActiveX.

Article Title: Distributed system for monitoring patient video, audio and medical parameter data
Article Snippet: In response to user selection of a particular patient in a clinical application display image provided on portable processing device 12 by user interface 26, a parameter is acquired from a medical record of a patient and provided in a URL data field to a Web-enabled plug-in (such as a Plug-in or other executable procedure, such as an ActiveX component) in server 20 (or in another embodiment in portable processing device 12), for example.

Article Title: Automatic selection of user-oriented web content
Article Snippet: The tool can take the form of a toolbar, plug-in, extension, or add-on to be installed in a browser, an ActiveX control, a stand-alone application, etc.

Article Title: Detection of malicious script operations using statistical analysis
Article Snippet: Generally, the security server 102 generates security data that describe whether a sequence of script operations performed by a script object(s) on a web page, or whether script operations performed by a browser add-on or extension object (e.g., an ActiveX control or plug-in), will cause a browser security vulnerability.

Article Title: System and method for sending, delivery and receiving of digital content
Article Snippet: In another embodiment, the sending connector 204 may be part of a web page (e.g., an applet, plug-in, ActiveX program, etc.) that is executing on a browser, where the browser may be rendering a web page that serves as transmission initiator 206.

Article Title: Method and system of facilitating automatic login to a web site using an Internet browser
Article Snippet: When an Internet user launches or activates a browser, the library file is opened and the ActiveX control or Plug-in code contained within that file is made available to the browser and incorporated into the browser interface, thus causing the interface object to be displayed with the browser interface.

Blocking Assay:

Article Title: Method and client for using an embedded ActiveX plug-in in a browser
Article Snippet: Therefore, it may be checked and judged whether the plug-in has already been installed in the computer system utilizing the correspondence between the class identifier and the ActiveX plug-in.

Article Title: Separate plug-in processes in browsers and applications thereof
Article Snippet: In this way, a plug-in instance is developed to understand one standard (such as NPAPI) may be able to access plug-ins implementing another standard (such as ActiveX).

Article Title: One-time dynamic positioning authentication method and system and password alteration method
Article Snippet: When displaying the full-element dynamic factor table, the client does not need to load or install any additional plug-in, e.g. ActiveX.

Article Title: Distributed system for monitoring patient video, audio and medical parameter data
Article Snippet: In response to user selection of a particular patient in a clinical application display image provided on portable processing device 12 by user interface 26, a parameter is acquired from a medical record of a patient and provided in a URL data field to a Web-enabled plug-in (such as a Plug-in or other executable procedure, such as an ActiveX component) in server 20 (or in another embodiment in portable processing device 12), for example.

Article Title: Automatic selection of user-oriented web content
Article Snippet: The tool can take the form of a toolbar, plug-in, extension, or add-on to be installed in a browser, an ActiveX control, a stand-alone application, etc.

Article Title: Detection of malicious script operations using statistical analysis
Article Snippet: Generally, the security server 102 generates security data that describe whether a sequence of script operations performed by a script object(s) on a web page, or whether script operations performed by a browser add-on or extension object (e.g., an ActiveX control or plug-in), will cause a browser security vulnerability.

Article Title: System and method for sending, delivery and receiving of digital content
Article Snippet: In another embodiment, the sending connector 204 may be part of a web page (e.g., an applet, plug-in, ActiveX program, etc.) that is executing on a browser, where the browser may be rendering a web page that serves as transmission initiator 206.

Article Title: Method and system of facilitating automatic login to a web site using an Internet browser
Article Snippet: When an Internet user launches or activates a browser, the library file is opened and the ActiveX control or Plug-in code contained within that file is made available to the browser and incorporated into the browser interface, thus causing the interface object to be displayed with the browser interface.

Sequencing:

Article Title: Method and client for using an embedded ActiveX plug-in in a browser
Article Snippet: Therefore, it may be checked and judged whether the plug-in has already been installed in the computer system utilizing the correspondence between the class identifier and the ActiveX plug-in.

Article Title: Separate plug-in processes in browsers and applications thereof
Article Snippet: In this way, a plug-in instance is developed to understand one standard (such as NPAPI) may be able to access plug-ins implementing another standard (such as ActiveX).

Article Title: One-time dynamic positioning authentication method and system and password alteration method
Article Snippet: When displaying the full-element dynamic factor table, the client does not need to load or install any additional plug-in, e.g. ActiveX.

Article Title: Distributed system for monitoring patient video, audio and medical parameter data
Article Snippet: In response to user selection of a particular patient in a clinical application display image provided on portable processing device 12 by user interface 26, a parameter is acquired from a medical record of a patient and provided in a URL data field to a Web-enabled plug-in (such as a Plug-in or other executable procedure, such as an ActiveX component) in server 20 (or in another embodiment in portable processing device 12), for example.

Article Title: Automatic selection of user-oriented web content
Article Snippet: The tool can take the form of a toolbar, plug-in, extension, or add-on to be installed in a browser, an ActiveX control, a stand-alone application, etc.

Article Title: Detection of malicious script operations using statistical analysis
Article Snippet: Generally, the security server 102 generates security data that describe whether a sequence of script operations performed by a script object(s) on a web page, or whether script operations performed by a browser add-on or extension object (e.g., an ActiveX control or plug-in), will cause a browser security vulnerability.

Article Title: System and method for sending, delivery and receiving of digital content
Article Snippet: In another embodiment, the sending connector 204 may be part of a web page (e.g., an applet, plug-in, ActiveX program, etc.) that is executing on a browser, where the browser may be rendering a web page that serves as transmission initiator 206.

Article Title: Method and system of facilitating automatic login to a web site using an Internet browser
Article Snippet: When an Internet user launches or activates a browser, the library file is opened and the ActiveX control or Plug-in code contained within that file is made available to the browser and incorporated into the browser interface, thus causing the interface object to be displayed with the browser interface.

Control:

Article Title: Method and client for using an embedded ActiveX plug-in in a browser
Article Snippet: Therefore, it may be checked and judged whether the plug-in has already been installed in the computer system utilizing the correspondence between the class identifier and the ActiveX plug-in.

Article Title: Separate plug-in processes in browsers and applications thereof
Article Snippet: In this way, a plug-in instance is developed to understand one standard (such as NPAPI) may be able to access plug-ins implementing another standard (such as ActiveX).

Article Title: One-time dynamic positioning authentication method and system and password alteration method
Article Snippet: When displaying the full-element dynamic factor table, the client does not need to load or install any additional plug-in, e.g. ActiveX.

Article Title: Distributed system for monitoring patient video, audio and medical parameter data
Article Snippet: In response to user selection of a particular patient in a clinical application display image provided on portable processing device 12 by user interface 26, a parameter is acquired from a medical record of a patient and provided in a URL data field to a Web-enabled plug-in (such as a Plug-in or other executable procedure, such as an ActiveX component) in server 20 (or in another embodiment in portable processing device 12), for example.

Article Title: Automatic selection of user-oriented web content
Article Snippet: The tool can take the form of a toolbar, plug-in, extension, or add-on to be installed in a browser, an ActiveX control, a stand-alone application, etc.

Article Title: Detection of malicious script operations using statistical analysis
Article Snippet: Generally, the security server 102 generates security data that describe whether a sequence of script operations performed by a script object(s) on a web page, or whether script operations performed by a browser add-on or extension object (e.g., an ActiveX control or plug-in), will cause a browser security vulnerability.

Article Title: System and method for sending, delivery and receiving of digital content
Article Snippet: In another embodiment, the sending connector 204 may be part of a web page (e.g., an applet, plug-in, ActiveX program, etc.) that is executing on a browser, where the browser may be rendering a web page that serves as transmission initiator 206.

Article Title: Method and system of facilitating automatic login to a web site using an Internet browser
Article Snippet: When an Internet user launches or activates a browser, the library file is opened and the ActiveX control or Plug-in code contained within that file is made available to the browser and incorporated into the browser interface, thus causing the interface object to be displayed with the browser interface.



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Comparison of vaccine efficacy between H5 Texas Mut vaccine and H5 Ast20 Mut vaccine in mice (A) Schematic of cleavage-defective mutant (Mut) HA mRNA vaccines. (B) Western blotting analysis of HA protein expression in transfected BHK-21 cells. Cells were transfected with varying doses (50, 100, 200, and 500 ng/μL) of Texas Mut or Ast20 Mut mRNA. (C) Mice were vaccinated (i.m.) with 1 or 5 μg Texas/Ast20 mRNA-LNP (SM102) encoding Mut HA or placebo using in a prime-boost schedule with an interval of 4 weeks. Blood samples ( n = 8) were collected from mice 2 weeks after prime and boost. Binding IgG of serum, reactive to the Texas HA protein, was measured by ELISA. Titers of placebo group were below detection limit. (D) 50% neutralization titers of pseudotyped virus (A/Texas/37/2024) in serum collected 2 weeks post-boost (week 6). Titers of placebo group were below detection limit. (E and F) Binding IgG of 1 μg Texas vaccine group (E) and Ast20 vaccine group (F) was measured by ELISA, reactive to the HA of clade 1 virus (A/Viet Nam/1203/2004), clade 2.1.3.2 virus <t>(A/Indonesia/5/2005),</t> clade 2.2 virus (A/bar-headed goose/Qinghai/5/2005), clade 2.3.2.1a virus (A/Hubei/1/2010), clade 2.3.4 virus (A/Anhui/1/2005), clade 2.3.4.4b virus (A/Astrakhan/3212/2020), and clade 2.3.4.4b virus (A/Texas/37/2024). (G) Radar chart of broad-binding IgG levels elicited by 1 μg Texas vaccine and Ast20 vaccine. (H) Vaccination schedule. Mice were vaccinated (i.m.) with 1 or 5 μg mRNA-LNP (SM102) encoding Texas HA-Mut, Ast20 HA-Mut, or placebo. Four weeks post-vaccination (week 4), mice were challenged with 50 × LD 50 of A/ostrich/China/HB/2024. Virus load of organs in vaccinated mice 1, 3, 5 days post-infection determined using plaque assays on MDCK cells. (I–L) Virus load of lung (I), heart (J), kidney (K), and brain (L). The dashed horizontal line indicates the assay limit of detection. Data are presented as the mean ± SD; p values are analyzed with t test (∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001) (C–L). ∗/∗∗∗ (E and F) indicates significant differences between other HA proteins and Texas (E) or Ast20 (F) protein. ∗ (E and F) indicates significant differences between other HA proteins and Texas (E) or Ast20 (F) protein. ∗ (I–L) indicates significant differences between placebo and vaccination groups in the same day.
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Comparison of vaccine efficacy between H5 Texas Mut vaccine and H5 Ast20 Mut vaccine in mice (A) Schematic of cleavage-defective mutant (Mut) HA mRNA vaccines. (B) Western blotting analysis of HA protein expression in transfected BHK-21 cells. Cells were transfected with varying doses (50, 100, 200, and 500 ng/μL) of Texas Mut or Ast20 Mut mRNA. (C) Mice were vaccinated (i.m.) with 1 or 5 μg Texas/Ast20 mRNA-LNP (SM102) encoding Mut HA or placebo using in a prime-boost schedule with an interval of 4 weeks. Blood samples ( n = 8) were collected from mice 2 weeks after prime and boost. Binding IgG of serum, reactive to the Texas HA protein, was measured by ELISA. Titers of placebo group were below detection limit. (D) 50% neutralization titers of pseudotyped virus (A/Texas/37/2024) in serum collected 2 weeks post-boost (week 6). Titers of placebo group were below detection limit. (E and F) Binding IgG of 1 μg Texas vaccine group (E) and Ast20 vaccine group (F) was measured by ELISA, reactive to the HA of clade 1 virus (A/Viet Nam/1203/2004), clade 2.1.3.2 virus <t>(A/Indonesia/5/2005),</t> clade 2.2 virus (A/bar-headed goose/Qinghai/5/2005), clade 2.3.2.1a virus (A/Hubei/1/2010), clade 2.3.4 virus (A/Anhui/1/2005), clade 2.3.4.4b virus (A/Astrakhan/3212/2020), and clade 2.3.4.4b virus (A/Texas/37/2024). (G) Radar chart of broad-binding IgG levels elicited by 1 μg Texas vaccine and Ast20 vaccine. (H) Vaccination schedule. Mice were vaccinated (i.m.) with 1 or 5 μg mRNA-LNP (SM102) encoding Texas HA-Mut, Ast20 HA-Mut, or placebo. Four weeks post-vaccination (week 4), mice were challenged with 50 × LD 50 of A/ostrich/China/HB/2024. Virus load of organs in vaccinated mice 1, 3, 5 days post-infection determined using plaque assays on MDCK cells. (I–L) Virus load of lung (I), heart (J), kidney (K), and brain (L). The dashed horizontal line indicates the assay limit of detection. Data are presented as the mean ± SD; p values are analyzed with t test (∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001) (C–L). ∗/∗∗∗ (E and F) indicates significant differences between other HA proteins and Texas (E) or Ast20 (F) protein. ∗ (E and F) indicates significant differences between other HA proteins and Texas (E) or Ast20 (F) protein. ∗ (I–L) indicates significant differences between placebo and vaccination groups in the same day.
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Statistik Georg Ferber cerita data statistik untuk indonesia
Comparison of vaccine efficacy between H5 Texas Mut vaccine and H5 Ast20 Mut vaccine in mice (A) Schematic of cleavage-defective mutant (Mut) HA mRNA vaccines. (B) Western blotting analysis of HA protein expression in transfected BHK-21 cells. Cells were transfected with varying doses (50, 100, 200, and 500 ng/μL) of Texas Mut or Ast20 Mut mRNA. (C) Mice were vaccinated (i.m.) with 1 or 5 μg Texas/Ast20 mRNA-LNP (SM102) encoding Mut HA or placebo using in a prime-boost schedule with an interval of 4 weeks. Blood samples ( n = 8) were collected from mice 2 weeks after prime and boost. Binding IgG of serum, reactive to the Texas HA protein, was measured by ELISA. Titers of placebo group were below detection limit. (D) 50% neutralization titers of pseudotyped virus (A/Texas/37/2024) in serum collected 2 weeks post-boost (week 6). Titers of placebo group were below detection limit. (E and F) Binding IgG of 1 μg Texas vaccine group (E) and Ast20 vaccine group (F) was measured by ELISA, reactive to the HA of clade 1 virus (A/Viet Nam/1203/2004), clade 2.1.3.2 virus <t>(A/Indonesia/5/2005),</t> clade 2.2 virus (A/bar-headed goose/Qinghai/5/2005), clade 2.3.2.1a virus (A/Hubei/1/2010), clade 2.3.4 virus (A/Anhui/1/2005), clade 2.3.4.4b virus (A/Astrakhan/3212/2020), and clade 2.3.4.4b virus (A/Texas/37/2024). (G) Radar chart of broad-binding IgG levels elicited by 1 μg Texas vaccine and Ast20 vaccine. (H) Vaccination schedule. Mice were vaccinated (i.m.) with 1 or 5 μg mRNA-LNP (SM102) encoding Texas HA-Mut, Ast20 HA-Mut, or placebo. Four weeks post-vaccination (week 4), mice were challenged with 50 × LD 50 of A/ostrich/China/HB/2024. Virus load of organs in vaccinated mice 1, 3, 5 days post-infection determined using plaque assays on MDCK cells. (I–L) Virus load of lung (I), heart (J), kidney (K), and brain (L). The dashed horizontal line indicates the assay limit of detection. Data are presented as the mean ± SD; p values are analyzed with t test (∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001) (C–L). ∗/∗∗∗ (E and F) indicates significant differences between other HA proteins and Texas (E) or Ast20 (F) protein. ∗ (E and F) indicates significant differences between other HA proteins and Texas (E) or Ast20 (F) protein. ∗ (I–L) indicates significant differences between placebo and vaccination groups in the same day.
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Comparison of vaccine efficacy between H5 Texas Mut vaccine and H5 Ast20 Mut vaccine in mice (A) Schematic of cleavage-defective mutant (Mut) HA mRNA vaccines. (B) Western blotting analysis of HA protein expression in transfected BHK-21 cells. Cells were transfected with varying doses (50, 100, 200, and 500 ng/μL) of Texas Mut or Ast20 Mut mRNA. (C) Mice were vaccinated (i.m.) with 1 or 5 μg Texas/Ast20 mRNA-LNP (SM102) encoding Mut HA or placebo using in a prime-boost schedule with an interval of 4 weeks. Blood samples ( n = 8) were collected from mice 2 weeks after prime and boost. Binding IgG of serum, reactive to the Texas HA protein, was measured by ELISA. Titers of placebo group were below detection limit. (D) 50% neutralization titers of pseudotyped virus (A/Texas/37/2024) in serum collected 2 weeks post-boost (week 6). Titers of placebo group were below detection limit. (E and F) Binding IgG of 1 μg Texas vaccine group (E) and Ast20 vaccine group (F) was measured by ELISA, reactive to the HA of clade 1 virus (A/Viet Nam/1203/2004), clade 2.1.3.2 virus (A/Indonesia/5/2005), clade 2.2 virus (A/bar-headed goose/Qinghai/5/2005), clade 2.3.2.1a virus (A/Hubei/1/2010), clade 2.3.4 virus (A/Anhui/1/2005), clade 2.3.4.4b virus (A/Astrakhan/3212/2020), and clade 2.3.4.4b virus (A/Texas/37/2024). (G) Radar chart of broad-binding IgG levels elicited by 1 μg Texas vaccine and Ast20 vaccine. (H) Vaccination schedule. Mice were vaccinated (i.m.) with 1 or 5 μg mRNA-LNP (SM102) encoding Texas HA-Mut, Ast20 HA-Mut, or placebo. Four weeks post-vaccination (week 4), mice were challenged with 50 × LD 50 of A/ostrich/China/HB/2024. Virus load of organs in vaccinated mice 1, 3, 5 days post-infection determined using plaque assays on MDCK cells. (I–L) Virus load of lung (I), heart (J), kidney (K), and brain (L). The dashed horizontal line indicates the assay limit of detection. Data are presented as the mean ± SD; p values are analyzed with t test (∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001) (C–L). ∗/∗∗∗ (E and F) indicates significant differences between other HA proteins and Texas (E) or Ast20 (F) protein. ∗ (E and F) indicates significant differences between other HA proteins and Texas (E) or Ast20 (F) protein. ∗ (I–L) indicates significant differences between placebo and vaccination groups in the same day.

Journal: Cell Reports Medicine

Article Title: Preclinical evaluation of an mRNA vaccine developed from the first human isolate of bovine H5N1

doi: 10.1016/j.xcrm.2026.102702

Figure Lengend Snippet: Comparison of vaccine efficacy between H5 Texas Mut vaccine and H5 Ast20 Mut vaccine in mice (A) Schematic of cleavage-defective mutant (Mut) HA mRNA vaccines. (B) Western blotting analysis of HA protein expression in transfected BHK-21 cells. Cells were transfected with varying doses (50, 100, 200, and 500 ng/μL) of Texas Mut or Ast20 Mut mRNA. (C) Mice were vaccinated (i.m.) with 1 or 5 μg Texas/Ast20 mRNA-LNP (SM102) encoding Mut HA or placebo using in a prime-boost schedule with an interval of 4 weeks. Blood samples ( n = 8) were collected from mice 2 weeks after prime and boost. Binding IgG of serum, reactive to the Texas HA protein, was measured by ELISA. Titers of placebo group were below detection limit. (D) 50% neutralization titers of pseudotyped virus (A/Texas/37/2024) in serum collected 2 weeks post-boost (week 6). Titers of placebo group were below detection limit. (E and F) Binding IgG of 1 μg Texas vaccine group (E) and Ast20 vaccine group (F) was measured by ELISA, reactive to the HA of clade 1 virus (A/Viet Nam/1203/2004), clade 2.1.3.2 virus (A/Indonesia/5/2005), clade 2.2 virus (A/bar-headed goose/Qinghai/5/2005), clade 2.3.2.1a virus (A/Hubei/1/2010), clade 2.3.4 virus (A/Anhui/1/2005), clade 2.3.4.4b virus (A/Astrakhan/3212/2020), and clade 2.3.4.4b virus (A/Texas/37/2024). (G) Radar chart of broad-binding IgG levels elicited by 1 μg Texas vaccine and Ast20 vaccine. (H) Vaccination schedule. Mice were vaccinated (i.m.) with 1 or 5 μg mRNA-LNP (SM102) encoding Texas HA-Mut, Ast20 HA-Mut, or placebo. Four weeks post-vaccination (week 4), mice were challenged with 50 × LD 50 of A/ostrich/China/HB/2024. Virus load of organs in vaccinated mice 1, 3, 5 days post-infection determined using plaque assays on MDCK cells. (I–L) Virus load of lung (I), heart (J), kidney (K), and brain (L). The dashed horizontal line indicates the assay limit of detection. Data are presented as the mean ± SD; p values are analyzed with t test (∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001) (C–L). ∗/∗∗∗ (E and F) indicates significant differences between other HA proteins and Texas (E) or Ast20 (F) protein. ∗ (E and F) indicates significant differences between other HA proteins and Texas (E) or Ast20 (F) protein. ∗ (I–L) indicates significant differences between placebo and vaccination groups in the same day.

Article Snippet: HA recombinant protein of A/Indonesia/5/2005 , Synthesized from Novoprotein , N/A.

Techniques: Comparison, Mutagenesis, Vaccines, Western Blot, Expressing, Transfection, Binding Assay, Enzyme-linked Immunosorbent Assay, Neutralization, Virus, Infection