Review




Structured Review

ActivX Inc activex container
Activex Container, supplied by ActivX Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/java+or+activex+software+components/activex+control+container/us07334166-112-14-12
Average 90 stars, based on 1 article reviews
activex container - by Bioz Stars, 2026-09
90/100 stars

Images

Related Articles

other:

Article Title: Video streaming technologies using ActiveX and LabVIEW
Article Snippet: From these containers, the ActiveX controls have their own methods and properties that can be used in order to manipulate the control.

Article Title: System and method for interoperability between an embedded activex control and an external application
Article Snippet: For example, an ActiveX Container, presented by ICHookCnt interface may provide client 335 with a representation of a set of ActiveX instances using a set of IActiveXWrapper objects.

Article Title: User interface for a system and process for providing dynamic communication access and information awareness in an interactive peripheral display
Article Snippet: In accordance with this working example, the main display surface for tickets is an ActiveX® container with prescribed interaction and implementation behavior between container and viewer ActiveX® servers, realized by deriving the ActiveX® servers interface contract from Ticket-interface.

Control:

Article Title: Manipulation of window controls in a popup window
Article Snippet: When the popup window launches and the applet loads, a notification is sent from the popup window to the ActiveX control container (620). .. The ActiveX control container then calls the system menu of the popup window and disables the “X” close window button (e.g., button 520) (630). .. The ActiveX control container also uses the system menu of the popup window to maximize the popup window (640).

Article Title: Syncronizing content blocks between multiple electronic documents
Article Snippet: .. For example, the container can be a Microsoft® OfficeTM comment or an ActiveX Control Container as known per se in the art. ..

Article Title: Syncronizing content blocks between multiple electronic documents
Article Snippet: .. For example, the container can be an ActiveX Control Container as known per se in the art. ..

Article Title: Manipulation of window controls in a popup window
Article Snippet: The ActiveX control container also uses the system menu of the popup window to maximize the popup window (640). .. Example code for an ActiveX control container in accordance with an embodiment is shown below: STDMETHODIMP CSSFlexAxContainerCtrl::Notify (NotifyType nType, ISSPropertySet* pData) { ............................ . if (m_bMaximizeWindow) { HWND hPopWnd = GetTopLevelParent( ); ::GetWindowPlacement(hPopWnd, ℘); wp.showCmd = SW_SHOWMAXIMIZED; ::SetWindowPlacement(hPopWnd, ℘); //Setting m_bMaximizeWindow to FALSE, so that the popup will //not be MAXIMIZED every time the function is getting called. m_bMaximizeWindow = FALSE; //Graying out X button in popup window hPopWnd = GetTopLevelParent( ); HMENU hSysMenu = ::GetSystemMenu(hPopWnd, FALSE); EnableMenuItem (hSysMenu, SC_CLOSE, MF_GRAYED); } ............................ . As disclosed, systems and methods for manipulating a browser window are disclosed. ..

Article Title: Manipulation of window controls in a popup window
Article Snippet: .. Accordingly, an embodiment embeds an ActiveX control container in the presentation popup window. .. When the popup window applet launches, a notification can be sent from the user interface (“UI”) to the ActiveX control container to disable the “X” close window button and to maximize the popup window.



Similar Products

86
Oracle Corporation oracle java cloud service jcs
Oracle Java Cloud Service Jcs, supplied by Oracle Corporation, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/java+or+activex+software+components/120+asset+cloud+device+digital+implements+management+oracle+service+system+utilities+%E2%80%9Cdacs/us12657208-430-16-6
Average 86 stars, based on 1 article reviews
oracle java cloud service jcs - by Bioz Stars, 2026-09
86/100 stars
  Buy from Supplier

86
Oracle Corporation enterprise java beans
Enterprise Java Beans, supplied by Oracle Corporation, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/java+or+activex+software+components/beans+enterprise+java/us12620123-208-17-22
Average 86 stars, based on 1 article reviews
enterprise java beans - by Bioz Stars, 2026-09
86/100 stars
  Buy from Supplier

86
Prodia Laboratories prodia stemcell indonesia
Prodia Stemcell Indonesia, supplied by Prodia Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/java+or+activex+software+components/indonesia+prodia+stemcell/pmc13166702-166-0-0
Average 86 stars, based on 1 article reviews
prodia stemcell indonesia - by Bioz Stars, 2026-09
86/100 stars
  Buy from Supplier

86
Novoprotein ha recombinant protein of a indonesia 5 2005
Comparison of vaccine efficacy between H5 Texas Mut vaccine and H5 Ast20 Mut vaccine in mice (A) Schematic of cleavage-defective mutant (Mut) HA mRNA vaccines. (B) Western blotting analysis of HA protein expression in transfected BHK-21 cells. Cells were transfected with varying doses (50, 100, 200, and 500 ng/μL) of Texas Mut or Ast20 Mut mRNA. (C) Mice were vaccinated (i.m.) with 1 or 5 μg Texas/Ast20 mRNA-LNP (SM102) encoding Mut HA or placebo using in a prime-boost schedule with an interval of 4 weeks. Blood samples ( n = 8) were collected from mice 2 weeks after prime and boost. Binding IgG of serum, reactive to the Texas HA protein, was measured by ELISA. Titers of placebo group were below detection limit. (D) 50% neutralization titers of pseudotyped virus (A/Texas/37/2024) in serum collected 2 weeks post-boost (week 6). Titers of placebo group were below detection limit. (E and F) Binding IgG of 1 μg Texas vaccine group (E) and Ast20 vaccine group (F) was measured by ELISA, reactive to the HA of clade 1 virus (A/Viet Nam/1203/2004), clade 2.1.3.2 virus <t>(A/Indonesia/5/2005),</t> clade 2.2 virus (A/bar-headed goose/Qinghai/5/2005), clade 2.3.2.1a virus (A/Hubei/1/2010), clade 2.3.4 virus (A/Anhui/1/2005), clade 2.3.4.4b virus (A/Astrakhan/3212/2020), and clade 2.3.4.4b virus (A/Texas/37/2024). (G) Radar chart of broad-binding IgG levels elicited by 1 μg Texas vaccine and Ast20 vaccine. (H) Vaccination schedule. Mice were vaccinated (i.m.) with 1 or 5 μg mRNA-LNP (SM102) encoding Texas HA-Mut, Ast20 HA-Mut, or placebo. Four weeks post-vaccination (week 4), mice were challenged with 50 × LD 50 of A/ostrich/China/HB/2024. Virus load of organs in vaccinated mice 1, 3, 5 days post-infection determined using plaque assays on MDCK cells. (I–L) Virus load of lung (I), heart (J), kidney (K), and brain (L). The dashed horizontal line indicates the assay limit of detection. Data are presented as the mean ± SD; p values are analyzed with t test (∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001) (C–L). ∗/∗∗∗ (E and F) indicates significant differences between other HA proteins and Texas (E) or Ast20 (F) protein. ∗ (E and F) indicates significant differences between other HA proteins and Texas (E) or Ast20 (F) protein. ∗ (I–L) indicates significant differences between placebo and vaccination groups in the same day.
Ha Recombinant Protein Of A Indonesia 5 2005, supplied by Novoprotein, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/java+or+activex+software+components/2005+5+a+bar+goose+ha+headed+of+protein+qinghai+recombinant/pmc13130686-29-0-8
Average 86 stars, based on 1 article reviews
ha recombinant protein of a indonesia 5 2005 - by Bioz Stars, 2026-09
86/100 stars
  Buy from Supplier

86
Insan Bamboo Salt jurnal insan farmasi indonesia
Comparison of vaccine efficacy between H5 Texas Mut vaccine and H5 Ast20 Mut vaccine in mice (A) Schematic of cleavage-defective mutant (Mut) HA mRNA vaccines. (B) Western blotting analysis of HA protein expression in transfected BHK-21 cells. Cells were transfected with varying doses (50, 100, 200, and 500 ng/μL) of Texas Mut or Ast20 Mut mRNA. (C) Mice were vaccinated (i.m.) with 1 or 5 μg Texas/Ast20 mRNA-LNP (SM102) encoding Mut HA or placebo using in a prime-boost schedule with an interval of 4 weeks. Blood samples ( n = 8) were collected from mice 2 weeks after prime and boost. Binding IgG of serum, reactive to the Texas HA protein, was measured by ELISA. Titers of placebo group were below detection limit. (D) 50% neutralization titers of pseudotyped virus (A/Texas/37/2024) in serum collected 2 weeks post-boost (week 6). Titers of placebo group were below detection limit. (E and F) Binding IgG of 1 μg Texas vaccine group (E) and Ast20 vaccine group (F) was measured by ELISA, reactive to the HA of clade 1 virus (A/Viet Nam/1203/2004), clade 2.1.3.2 virus <t>(A/Indonesia/5/2005),</t> clade 2.2 virus (A/bar-headed goose/Qinghai/5/2005), clade 2.3.2.1a virus (A/Hubei/1/2010), clade 2.3.4 virus (A/Anhui/1/2005), clade 2.3.4.4b virus (A/Astrakhan/3212/2020), and clade 2.3.4.4b virus (A/Texas/37/2024). (G) Radar chart of broad-binding IgG levels elicited by 1 μg Texas vaccine and Ast20 vaccine. (H) Vaccination schedule. Mice were vaccinated (i.m.) with 1 or 5 μg mRNA-LNP (SM102) encoding Texas HA-Mut, Ast20 HA-Mut, or placebo. Four weeks post-vaccination (week 4), mice were challenged with 50 × LD 50 of A/ostrich/China/HB/2024. Virus load of organs in vaccinated mice 1, 3, 5 days post-infection determined using plaque assays on MDCK cells. (I–L) Virus load of lung (I), heart (J), kidney (K), and brain (L). The dashed horizontal line indicates the assay limit of detection. Data are presented as the mean ± SD; p values are analyzed with t test (∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001) (C–L). ∗/∗∗∗ (E and F) indicates significant differences between other HA proteins and Texas (E) or Ast20 (F) protein. ∗ (E and F) indicates significant differences between other HA proteins and Texas (E) or Ast20 (F) protein. ∗ (I–L) indicates significant differences between placebo and vaccination groups in the same day.
Jurnal Insan Farmasi Indonesia, supplied by Insan Bamboo Salt, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/java+or+activex+software+components/farmasi+indonesia+insan+jurnal/10__55262_slash_fabadeczacilik__1752400-299-0-1
Average 86 stars, based on 1 article reviews
jurnal insan farmasi indonesia - by Bioz Stars, 2026-09
86/100 stars
  Buy from Supplier

86
Statistik Georg Ferber statistik indonesia
Comparison of vaccine efficacy between H5 Texas Mut vaccine and H5 Ast20 Mut vaccine in mice (A) Schematic of cleavage-defective mutant (Mut) HA mRNA vaccines. (B) Western blotting analysis of HA protein expression in transfected BHK-21 cells. Cells were transfected with varying doses (50, 100, 200, and 500 ng/μL) of Texas Mut or Ast20 Mut mRNA. (C) Mice were vaccinated (i.m.) with 1 or 5 μg Texas/Ast20 mRNA-LNP (SM102) encoding Mut HA or placebo using in a prime-boost schedule with an interval of 4 weeks. Blood samples ( n = 8) were collected from mice 2 weeks after prime and boost. Binding IgG of serum, reactive to the Texas HA protein, was measured by ELISA. Titers of placebo group were below detection limit. (D) 50% neutralization titers of pseudotyped virus (A/Texas/37/2024) in serum collected 2 weeks post-boost (week 6). Titers of placebo group were below detection limit. (E and F) Binding IgG of 1 μg Texas vaccine group (E) and Ast20 vaccine group (F) was measured by ELISA, reactive to the HA of clade 1 virus (A/Viet Nam/1203/2004), clade 2.1.3.2 virus <t>(A/Indonesia/5/2005),</t> clade 2.2 virus (A/bar-headed goose/Qinghai/5/2005), clade 2.3.2.1a virus (A/Hubei/1/2010), clade 2.3.4 virus (A/Anhui/1/2005), clade 2.3.4.4b virus (A/Astrakhan/3212/2020), and clade 2.3.4.4b virus (A/Texas/37/2024). (G) Radar chart of broad-binding IgG levels elicited by 1 μg Texas vaccine and Ast20 vaccine. (H) Vaccination schedule. Mice were vaccinated (i.m.) with 1 or 5 μg mRNA-LNP (SM102) encoding Texas HA-Mut, Ast20 HA-Mut, or placebo. Four weeks post-vaccination (week 4), mice were challenged with 50 × LD 50 of A/ostrich/China/HB/2024. Virus load of organs in vaccinated mice 1, 3, 5 days post-infection determined using plaque assays on MDCK cells. (I–L) Virus load of lung (I), heart (J), kidney (K), and brain (L). The dashed horizontal line indicates the assay limit of detection. Data are presented as the mean ± SD; p values are analyzed with t test (∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001) (C–L). ∗/∗∗∗ (E and F) indicates significant differences between other HA proteins and Texas (E) or Ast20 (F) protein. ∗ (E and F) indicates significant differences between other HA proteins and Texas (E) or Ast20 (F) protein. ∗ (I–L) indicates significant differences between placebo and vaccination groups in the same day.
Statistik Indonesia, supplied by Statistik Georg Ferber, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/java+or+activex+software+components/indonesia+statistik/pm41906164-559-3-3
Average 86 stars, based on 1 article reviews
statistik indonesia - by Bioz Stars, 2026-09
86/100 stars
  Buy from Supplier

86
Statistik Georg Ferber statistik indonesia 2023
Comparison of vaccine efficacy between H5 Texas Mut vaccine and H5 Ast20 Mut vaccine in mice (A) Schematic of cleavage-defective mutant (Mut) HA mRNA vaccines. (B) Western blotting analysis of HA protein expression in transfected BHK-21 cells. Cells were transfected with varying doses (50, 100, 200, and 500 ng/μL) of Texas Mut or Ast20 Mut mRNA. (C) Mice were vaccinated (i.m.) with 1 or 5 μg Texas/Ast20 mRNA-LNP (SM102) encoding Mut HA or placebo using in a prime-boost schedule with an interval of 4 weeks. Blood samples ( n = 8) were collected from mice 2 weeks after prime and boost. Binding IgG of serum, reactive to the Texas HA protein, was measured by ELISA. Titers of placebo group were below detection limit. (D) 50% neutralization titers of pseudotyped virus (A/Texas/37/2024) in serum collected 2 weeks post-boost (week 6). Titers of placebo group were below detection limit. (E and F) Binding IgG of 1 μg Texas vaccine group (E) and Ast20 vaccine group (F) was measured by ELISA, reactive to the HA of clade 1 virus (A/Viet Nam/1203/2004), clade 2.1.3.2 virus <t>(A/Indonesia/5/2005),</t> clade 2.2 virus (A/bar-headed goose/Qinghai/5/2005), clade 2.3.2.1a virus (A/Hubei/1/2010), clade 2.3.4 virus (A/Anhui/1/2005), clade 2.3.4.4b virus (A/Astrakhan/3212/2020), and clade 2.3.4.4b virus (A/Texas/37/2024). (G) Radar chart of broad-binding IgG levels elicited by 1 μg Texas vaccine and Ast20 vaccine. (H) Vaccination schedule. Mice were vaccinated (i.m.) with 1 or 5 μg mRNA-LNP (SM102) encoding Texas HA-Mut, Ast20 HA-Mut, or placebo. Four weeks post-vaccination (week 4), mice were challenged with 50 × LD 50 of A/ostrich/China/HB/2024. Virus load of organs in vaccinated mice 1, 3, 5 days post-infection determined using plaque assays on MDCK cells. (I–L) Virus load of lung (I), heart (J), kidney (K), and brain (L). The dashed horizontal line indicates the assay limit of detection. Data are presented as the mean ± SD; p values are analyzed with t test (∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001) (C–L). ∗/∗∗∗ (E and F) indicates significant differences between other HA proteins and Texas (E) or Ast20 (F) protein. ∗ (E and F) indicates significant differences between other HA proteins and Texas (E) or Ast20 (F) protein. ∗ (I–L) indicates significant differences between placebo and vaccination groups in the same day.
Statistik Indonesia 2023, supplied by Statistik Georg Ferber, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/java+or+activex+software+components/2023+indonesia+statistik/pm41906164-420-0-0
Average 86 stars, based on 1 article reviews
statistik indonesia 2023 - by Bioz Stars, 2026-09
86/100 stars
  Buy from Supplier

86
Statistik Georg Ferber statistik indonesia 2024
Comparison of vaccine efficacy between H5 Texas Mut vaccine and H5 Ast20 Mut vaccine in mice (A) Schematic of cleavage-defective mutant (Mut) HA mRNA vaccines. (B) Western blotting analysis of HA protein expression in transfected BHK-21 cells. Cells were transfected with varying doses (50, 100, 200, and 500 ng/μL) of Texas Mut or Ast20 Mut mRNA. (C) Mice were vaccinated (i.m.) with 1 or 5 μg Texas/Ast20 mRNA-LNP (SM102) encoding Mut HA or placebo using in a prime-boost schedule with an interval of 4 weeks. Blood samples ( n = 8) were collected from mice 2 weeks after prime and boost. Binding IgG of serum, reactive to the Texas HA protein, was measured by ELISA. Titers of placebo group were below detection limit. (D) 50% neutralization titers of pseudotyped virus (A/Texas/37/2024) in serum collected 2 weeks post-boost (week 6). Titers of placebo group were below detection limit. (E and F) Binding IgG of 1 μg Texas vaccine group (E) and Ast20 vaccine group (F) was measured by ELISA, reactive to the HA of clade 1 virus (A/Viet Nam/1203/2004), clade 2.1.3.2 virus <t>(A/Indonesia/5/2005),</t> clade 2.2 virus (A/bar-headed goose/Qinghai/5/2005), clade 2.3.2.1a virus (A/Hubei/1/2010), clade 2.3.4 virus (A/Anhui/1/2005), clade 2.3.4.4b virus (A/Astrakhan/3212/2020), and clade 2.3.4.4b virus (A/Texas/37/2024). (G) Radar chart of broad-binding IgG levels elicited by 1 μg Texas vaccine and Ast20 vaccine. (H) Vaccination schedule. Mice were vaccinated (i.m.) with 1 or 5 μg mRNA-LNP (SM102) encoding Texas HA-Mut, Ast20 HA-Mut, or placebo. Four weeks post-vaccination (week 4), mice were challenged with 50 × LD 50 of A/ostrich/China/HB/2024. Virus load of organs in vaccinated mice 1, 3, 5 days post-infection determined using plaque assays on MDCK cells. (I–L) Virus load of lung (I), heart (J), kidney (K), and brain (L). The dashed horizontal line indicates the assay limit of detection. Data are presented as the mean ± SD; p values are analyzed with t test (∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001) (C–L). ∗/∗∗∗ (E and F) indicates significant differences between other HA proteins and Texas (E) or Ast20 (F) protein. ∗ (E and F) indicates significant differences between other HA proteins and Texas (E) or Ast20 (F) protein. ∗ (I–L) indicates significant differences between placebo and vaccination groups in the same day.
Statistik Indonesia 2024, supplied by Statistik Georg Ferber, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/java+or+activex+software+components/2023+indonesia+statistik/10__64118_slash_aj__v3i1__71-129-0-0
Average 86 stars, based on 1 article reviews
statistik indonesia 2024 - by Bioz Stars, 2026-09
86/100 stars
  Buy from Supplier

86
Statistik Georg Ferber cerita data statistik untuk indonesia
Comparison of vaccine efficacy between H5 Texas Mut vaccine and H5 Ast20 Mut vaccine in mice (A) Schematic of cleavage-defective mutant (Mut) HA mRNA vaccines. (B) Western blotting analysis of HA protein expression in transfected BHK-21 cells. Cells were transfected with varying doses (50, 100, 200, and 500 ng/μL) of Texas Mut or Ast20 Mut mRNA. (C) Mice were vaccinated (i.m.) with 1 or 5 μg Texas/Ast20 mRNA-LNP (SM102) encoding Mut HA or placebo using in a prime-boost schedule with an interval of 4 weeks. Blood samples ( n = 8) were collected from mice 2 weeks after prime and boost. Binding IgG of serum, reactive to the Texas HA protein, was measured by ELISA. Titers of placebo group were below detection limit. (D) 50% neutralization titers of pseudotyped virus (A/Texas/37/2024) in serum collected 2 weeks post-boost (week 6). Titers of placebo group were below detection limit. (E and F) Binding IgG of 1 μg Texas vaccine group (E) and Ast20 vaccine group (F) was measured by ELISA, reactive to the HA of clade 1 virus (A/Viet Nam/1203/2004), clade 2.1.3.2 virus <t>(A/Indonesia/5/2005),</t> clade 2.2 virus (A/bar-headed goose/Qinghai/5/2005), clade 2.3.2.1a virus (A/Hubei/1/2010), clade 2.3.4 virus (A/Anhui/1/2005), clade 2.3.4.4b virus (A/Astrakhan/3212/2020), and clade 2.3.4.4b virus (A/Texas/37/2024). (G) Radar chart of broad-binding IgG levels elicited by 1 μg Texas vaccine and Ast20 vaccine. (H) Vaccination schedule. Mice were vaccinated (i.m.) with 1 or 5 μg mRNA-LNP (SM102) encoding Texas HA-Mut, Ast20 HA-Mut, or placebo. Four weeks post-vaccination (week 4), mice were challenged with 50 × LD 50 of A/ostrich/China/HB/2024. Virus load of organs in vaccinated mice 1, 3, 5 days post-infection determined using plaque assays on MDCK cells. (I–L) Virus load of lung (I), heart (J), kidney (K), and brain (L). The dashed horizontal line indicates the assay limit of detection. Data are presented as the mean ± SD; p values are analyzed with t test (∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001) (C–L). ∗/∗∗∗ (E and F) indicates significant differences between other HA proteins and Texas (E) or Ast20 (F) protein. ∗ (E and F) indicates significant differences between other HA proteins and Texas (E) or Ast20 (F) protein. ∗ (I–L) indicates significant differences between placebo and vaccination groups in the same day.
Cerita Data Statistik Untuk Indonesia, supplied by Statistik Georg Ferber, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/java+or+activex+software+components/atau+berdistribusi+berdistribusi+data+data+digunakan+diketahui+hipotesis+maka+menguji+normal+normal+setelah+tersebut+tidak+untuk/10__57178_slash_paradoks__v9i1__2158-338-0-2
Average 86 stars, based on 1 article reviews
cerita data statistik untuk indonesia - by Bioz Stars, 2026-09
86/100 stars
  Buy from Supplier

Image Search Results


Comparison of vaccine efficacy between H5 Texas Mut vaccine and H5 Ast20 Mut vaccine in mice (A) Schematic of cleavage-defective mutant (Mut) HA mRNA vaccines. (B) Western blotting analysis of HA protein expression in transfected BHK-21 cells. Cells were transfected with varying doses (50, 100, 200, and 500 ng/μL) of Texas Mut or Ast20 Mut mRNA. (C) Mice were vaccinated (i.m.) with 1 or 5 μg Texas/Ast20 mRNA-LNP (SM102) encoding Mut HA or placebo using in a prime-boost schedule with an interval of 4 weeks. Blood samples ( n = 8) were collected from mice 2 weeks after prime and boost. Binding IgG of serum, reactive to the Texas HA protein, was measured by ELISA. Titers of placebo group were below detection limit. (D) 50% neutralization titers of pseudotyped virus (A/Texas/37/2024) in serum collected 2 weeks post-boost (week 6). Titers of placebo group were below detection limit. (E and F) Binding IgG of 1 μg Texas vaccine group (E) and Ast20 vaccine group (F) was measured by ELISA, reactive to the HA of clade 1 virus (A/Viet Nam/1203/2004), clade 2.1.3.2 virus (A/Indonesia/5/2005), clade 2.2 virus (A/bar-headed goose/Qinghai/5/2005), clade 2.3.2.1a virus (A/Hubei/1/2010), clade 2.3.4 virus (A/Anhui/1/2005), clade 2.3.4.4b virus (A/Astrakhan/3212/2020), and clade 2.3.4.4b virus (A/Texas/37/2024). (G) Radar chart of broad-binding IgG levels elicited by 1 μg Texas vaccine and Ast20 vaccine. (H) Vaccination schedule. Mice were vaccinated (i.m.) with 1 or 5 μg mRNA-LNP (SM102) encoding Texas HA-Mut, Ast20 HA-Mut, or placebo. Four weeks post-vaccination (week 4), mice were challenged with 50 × LD 50 of A/ostrich/China/HB/2024. Virus load of organs in vaccinated mice 1, 3, 5 days post-infection determined using plaque assays on MDCK cells. (I–L) Virus load of lung (I), heart (J), kidney (K), and brain (L). The dashed horizontal line indicates the assay limit of detection. Data are presented as the mean ± SD; p values are analyzed with t test (∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001) (C–L). ∗/∗∗∗ (E and F) indicates significant differences between other HA proteins and Texas (E) or Ast20 (F) protein. ∗ (E and F) indicates significant differences between other HA proteins and Texas (E) or Ast20 (F) protein. ∗ (I–L) indicates significant differences between placebo and vaccination groups in the same day.

Journal: Cell Reports Medicine

Article Title: Preclinical evaluation of an mRNA vaccine developed from the first human isolate of bovine H5N1

doi: 10.1016/j.xcrm.2026.102702

Figure Lengend Snippet: Comparison of vaccine efficacy between H5 Texas Mut vaccine and H5 Ast20 Mut vaccine in mice (A) Schematic of cleavage-defective mutant (Mut) HA mRNA vaccines. (B) Western blotting analysis of HA protein expression in transfected BHK-21 cells. Cells were transfected with varying doses (50, 100, 200, and 500 ng/μL) of Texas Mut or Ast20 Mut mRNA. (C) Mice were vaccinated (i.m.) with 1 or 5 μg Texas/Ast20 mRNA-LNP (SM102) encoding Mut HA or placebo using in a prime-boost schedule with an interval of 4 weeks. Blood samples ( n = 8) were collected from mice 2 weeks after prime and boost. Binding IgG of serum, reactive to the Texas HA protein, was measured by ELISA. Titers of placebo group were below detection limit. (D) 50% neutralization titers of pseudotyped virus (A/Texas/37/2024) in serum collected 2 weeks post-boost (week 6). Titers of placebo group were below detection limit. (E and F) Binding IgG of 1 μg Texas vaccine group (E) and Ast20 vaccine group (F) was measured by ELISA, reactive to the HA of clade 1 virus (A/Viet Nam/1203/2004), clade 2.1.3.2 virus (A/Indonesia/5/2005), clade 2.2 virus (A/bar-headed goose/Qinghai/5/2005), clade 2.3.2.1a virus (A/Hubei/1/2010), clade 2.3.4 virus (A/Anhui/1/2005), clade 2.3.4.4b virus (A/Astrakhan/3212/2020), and clade 2.3.4.4b virus (A/Texas/37/2024). (G) Radar chart of broad-binding IgG levels elicited by 1 μg Texas vaccine and Ast20 vaccine. (H) Vaccination schedule. Mice were vaccinated (i.m.) with 1 or 5 μg mRNA-LNP (SM102) encoding Texas HA-Mut, Ast20 HA-Mut, or placebo. Four weeks post-vaccination (week 4), mice were challenged with 50 × LD 50 of A/ostrich/China/HB/2024. Virus load of organs in vaccinated mice 1, 3, 5 days post-infection determined using plaque assays on MDCK cells. (I–L) Virus load of lung (I), heart (J), kidney (K), and brain (L). The dashed horizontal line indicates the assay limit of detection. Data are presented as the mean ± SD; p values are analyzed with t test (∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001) (C–L). ∗/∗∗∗ (E and F) indicates significant differences between other HA proteins and Texas (E) or Ast20 (F) protein. ∗ (E and F) indicates significant differences between other HA proteins and Texas (E) or Ast20 (F) protein. ∗ (I–L) indicates significant differences between placebo and vaccination groups in the same day.

Article Snippet: HA recombinant protein of A/Indonesia/5/2005 , Synthesized from Novoprotein , N/A.

Techniques: Comparison, Mutagenesis, Vaccines, Western Blot, Expressing, Transfection, Binding Assay, Enzyme-linked Immunosorbent Assay, Neutralization, Virus, Infection