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Promega nuclei isolation buffer 0.1% triton-x
Nuclei Isolation Buffer 0.1% Triton X, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/isolation+buffer/nuclei+isolation+buffer+0+1++triton+x/pmc04465030-101-19-47
Average 90 stars, based on 1 article reviews
nuclei isolation buffer 0.1% triton-x - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Nuclear Run-on Assay:

Article Title: Alteration of mammary gland development and gene expression by in utero exposure to arsenic
Article Snippet: Samples were then run on the 7900HT (Applied Biosystems) and the data analyzed by the 2 −ΔΔCt method using the SDS 2.1 software (Applied Biosystems). .. Nuclear run-on assay For the preparation of nuclei, mammary glands were frozen, pulverized in liquid nitrogen, and resuspended in nuclei isolation buffer (15mM HEPES, 1.5M sucrose, 60mM KCl, 15mM NaCl, 0.15mM spermine, 0.5mM spermidine, 2mM EDTA, 0.5mM EGTA, 0.1mM PMSF*, 2mM DTT*; *added fresh) plus 0.1% Triton-X (Promega). ..

Isolation:

Article Title: Alteration of mammary gland development and gene expression by in utero exposure to arsenic
Article Snippet: Samples were then run on the 7900HT (Applied Biosystems) and the data analyzed by the 2 −ΔΔCt method using the SDS 2.1 software (Applied Biosystems). .. Nuclear run-on assay For the preparation of nuclei, mammary glands were frozen, pulverized in liquid nitrogen, and resuspended in nuclei isolation buffer (15mM HEPES, 1.5M sucrose, 60mM KCl, 15mM NaCl, 0.15mM spermine, 0.5mM spermidine, 2mM EDTA, 0.5mM EGTA, 0.1mM PMSF*, 2mM DTT*; *added fresh) plus 0.1% Triton-X (Promega). ..



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