2 layer percoll (Danaher Inc)
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97
Structured Review
Danaher Inc
2 layer percoll
2 Layer Percoll, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 97/100, based on 9372 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/isolate+2-layer+gradient+system/Percoll/pm33450198-252-34-37
Average 97 stars, based on 9372 article reviews
2 Layer Percoll, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 97/100, based on 9372 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/isolate+2-layer+gradient+system/Percoll/pm33450198-252-34-37
Average 97 stars, based on 9372 article reviews
2 layer percoll - by Bioz Stars,
2026-11
97/100 stars
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other:Article Title: Diet-mediated constitutive induction of novel IL-4 + ILC2 cells maintains intestinal homeostasis in mice Article Snippet: An aliquot of epithelial cells was washed with 10 ml of HBSS containing 5 mM EDTA, resuspended in 5 ml of 20% Percoll (GE Healthcare), and underlaid with 2.5 ml of 40% Percoll in a 15 ml Falcon tube to isolate colonic tuft cells. Article Title: Diet-mediated constitutive induction of novel IL-4 + ILC2 cells maintains intestinal homeostasis in mice Article Snippet: The digested tissues were washed with 10 ml of HBSS containing 5 mM EDTA, resuspended in 5 ml of 40% Percoll (GE Healthcare), and underlaid with 2.5 ml of 80% Percoll in a 15 ml Falcon tube. Gradient Centrifugation:Article Title: TRIM32 reduced the recruitment of innate immune cells and the killing capacity of Listeria monocytogenes by inhibiting secretion of chemokines Article Snippet: Livers were minced through a 100 μm cell strainer and digested for 30 min at 37 °C with rotation in HBSS (Ca 2+ , Mg 2+ ) buffer containing 1 mg/ml (335 U/mg) collagenase IV (Sangon Biotech, Shanghai, China) and 200 μg/ml (100 Kunitz U/ml) DNase I (Sangon Biotech, Shanghai, China). .. Liver leukocytes were separated by Isolation:Article Title: A diet rich in fermentable fiber promotes robust changes in the intestinal microbiota, mitigates intestinal permeability, and attenuates autoimmune uveitis Article Snippet: The cecum and colon were cut into 1 cm-long pieces and were digested in RPMI media with 5 mM EDTA (37 °C, 30 min × 2) and subsequently with 0.1 mg/ml collagenase II (Sigma-Aldrich) and 0.1 mg/ml DNase I (Roche, San Francisco, CA, USA) at 37 °C for 45 min twice (total 90 min). .. LPL were isolated after resuspending digested intestinal tissues in 30% Purification:Article Title: Functional virus-specific memory T cells survey glioblastoma Article Snippet: .. Tumors were minced and digested in Collagenase type IV with DNAse for 30 min then dissociated via gentleMACS Dissociator, and lymphocytes purified on a 44/67% Article Title: Functional virus-specific memory T cells survey glioblastoma Article Snippet: .. In brief, GBM tumors were removed, digested in Collagenase IV (Sigma) with DNAse for 30 min, then dissociated via gentleMACS Dissociator (Miltenyi Biotec) and lymphocytes purified on a 44/67% |