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qscript cdna supermix kit  (Quanta Biosciences)


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    Structured Review

    Quanta Biosciences qscript cdna supermix kit
    Qscript Cdna Supermix Kit, supplied by Quanta Biosciences, used in various techniques. Bioz Stars score: 97/100, based on 4396 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/iscript+reverse+transcriptase+rt+kit/qScript+cDNA+SuperMix/pm37075982-165-21-25
    Average 97 stars, based on 4396 article reviews
    qscript cdna supermix kit - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: Hemoglobin Nanofibrils as Electrospun Cell Scaffolds to Enhance Primary Satellite Cell Proliferation and Differentiation for Muscle Regeneration.
    Article Snippet: See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense 5 of 12Journal of Biomedical Materials Research Part A, 2026 Spectrophotometer (ThermoFisher Scientific, US), and its purity was verified by absorbance the 260 nm/280 nm ratio. .. Complementary DNA (cDNA) was reverse- transcribed from the extracted RNA using qScript XLT cDNA SuperMix (Cat # 95048- 100, QuantaBio) at a volume ratio of 4:1 in a Veriti Thermal Cycler (Applied Biosystems, Thermo Fisher Scientific, US), with cycle settings of 25°C for 5 min, 42°C for 60 min, and 85°C for 5 min. ..

    Article Title: Molecular signature
    Article Snippet: .. Real Time PCR Validation For microarray validation, total RNA from the 33 pre-invasive LUSC lesions undergoing Illumina gene expression profiling was reverse transcribed using qScript® cDNA Super-Mix (Polymerase chain reaction (PCR) reagents) (Quanta Biosciences, Lutterworth, UK) according to the manufacturer's protocol. .. Real-time quantitative PCR was carried out in eight genes using the SYBR-green master mix (Applied BioSystems, Bleiswijk, Netherlands) in an Eppendorf real-time PCR Machine (Eppendorf, Stevenage, UK).

    Article Title: MECP2 mutations rewire human ESC fate and bias cortical lineage commitment.
    Article Snippet: Total RNA was isolated from each hiPSC line in triplicate (n = 3 biological replicates per genotype) with the RNeasy Mini Kit (Qiagen, #74106) on a QIAcube automated workstation. .. For cDNA synthesis, 500 ng of RNA were reverse-transcribed using qScript cDNA SuperMix (Quantabio, #95048-100) on a SimpliAmp thermal cycler, following the manufacturer’s protocol. ..

    Synthesized:

    Article Title: Engineered commensals for metabolic modulation of the gut-liver-brain axis.
    Article Snippet: .. Bacterial cells were harvested and RNA extracted using Trizol Reagent (Thermo Fisher Scientific, USA, Cat# 15596018) following the manufacturer’s protocol. cDNA was synthesized from RNA (1 μg) using qScript cDNA SuperMix (Quantabio, USA, Cat# 95048) following the manufacturer’s protocol. qRT-PCR was performed using 2X-diluted cDNA template using Luna Universal qPCR Master Mix (New England Biolabs, USA, Cat# M3003L) following the manufacturer’s protocol. ..

    Quantitative RT-PCR:

    Article Title: Engineered commensals for metabolic modulation of the gut-liver-brain axis.
    Article Snippet: .. Bacterial cells were harvested and RNA extracted using Trizol Reagent (Thermo Fisher Scientific, USA, Cat# 15596018) following the manufacturer’s protocol. cDNA was synthesized from RNA (1 μg) using qScript cDNA SuperMix (Quantabio, USA, Cat# 95048) following the manufacturer’s protocol. qRT-PCR was performed using 2X-diluted cDNA template using Luna Universal qPCR Master Mix (New England Biolabs, USA, Cat# M3003L) following the manufacturer’s protocol. ..

    Article Title: Tunable tau expression in C. elegans neurons reveals that early-AD tau phosphorylation selectively impacts behavior and mitochondrial quality control
    Article Snippet: Contaminating genomic DNA was digested using a Turbo DNA-free TM Kit (AM1907, Invitrogen, Carlsbad, CA), the RNA concentration of each sample was measured via NanoDrop (LT1022, Thermo Scientific, Waltham, MA). .. 300 ng RNA was used to create cDNA libraries from each sample using Quantabio qScript cDNA Supermix (95,048, Quantabio, Beverly, MA). qRT-PCR was run in 96-well plates using 2× Universal SYBR Green Fast qPCR Mix (RK21203, ABclonal, Woburn, MA) and the primers listed in . .. All statistical analyses were conducted using Prism 8.0 (GraphPad Software Inc., Boston, MA), with alpha-error level of p < 0.05 considered to be significant.

    Real-time Polymerase Chain Reaction:

    Article Title: Engineered commensals for metabolic modulation of the gut-liver-brain axis.
    Article Snippet: .. Bacterial cells were harvested and RNA extracted using Trizol Reagent (Thermo Fisher Scientific, USA, Cat# 15596018) following the manufacturer’s protocol. cDNA was synthesized from RNA (1 μg) using qScript cDNA SuperMix (Quantabio, USA, Cat# 95048) following the manufacturer’s protocol. qRT-PCR was performed using 2X-diluted cDNA template using Luna Universal qPCR Master Mix (New England Biolabs, USA, Cat# M3003L) following the manufacturer’s protocol. ..

    Article Title: Tunable tau expression in C. elegans neurons reveals that early-AD tau phosphorylation selectively impacts behavior and mitochondrial quality control
    Article Snippet: Contaminating genomic DNA was digested using a Turbo DNA-free TM Kit (AM1907, Invitrogen, Carlsbad, CA), the RNA concentration of each sample was measured via NanoDrop (LT1022, Thermo Scientific, Waltham, MA). .. 300 ng RNA was used to create cDNA libraries from each sample using Quantabio qScript cDNA Supermix (95,048, Quantabio, Beverly, MA). qRT-PCR was run in 96-well plates using 2× Universal SYBR Green Fast qPCR Mix (RK21203, ABclonal, Woburn, MA) and the primers listed in . .. All statistical analyses were conducted using Prism 8.0 (GraphPad Software Inc., Boston, MA), with alpha-error level of p < 0.05 considered to be significant.

    Article Title: Molecular signature
    Article Snippet: .. Real Time PCR Validation For microarray validation, total RNA from the 33 pre-invasive LUSC lesions undergoing Illumina gene expression profiling was reverse transcribed using qScript® cDNA Super-Mix (Polymerase chain reaction (PCR) reagents) (Quanta Biosciences, Lutterworth, UK) according to the manufacturer's protocol. .. Real-time quantitative PCR was carried out in eight genes using the SYBR-green master mix (Applied BioSystems, Bleiswijk, Netherlands) in an Eppendorf real-time PCR Machine (Eppendorf, Stevenage, UK).

    Concentration Assay:

    Article Title: Exercise volume modulates cardiac protection in a type 2 diabetic rat model: differential effects of high- and low-volume moderate-intensity endurance exercise training on diabetic cardiomyopathy
    Article Snippet: Total RNA isolation was performed using an RNeasy Fibrous Tissue Kit (Qiagen Benelux B.V., Antwerp, Belgium), following the manufacturer’s protocol. .. RNA concentration and purity were assessed using a NanoDrop 2000 spectrophotometer (Isogen Life Science B.V., Utrecht, Netherlands). cDNA synthesis was performed using a qScript cDNA SuperMix (QuantaBio, Leuven, Belgium). ..

    Spectrophotometry:

    Article Title: Exercise volume modulates cardiac protection in a type 2 diabetic rat model: differential effects of high- and low-volume moderate-intensity endurance exercise training on diabetic cardiomyopathy
    Article Snippet: Total RNA isolation was performed using an RNeasy Fibrous Tissue Kit (Qiagen Benelux B.V., Antwerp, Belgium), following the manufacturer’s protocol. .. RNA concentration and purity were assessed using a NanoDrop 2000 spectrophotometer (Isogen Life Science B.V., Utrecht, Netherlands). cDNA synthesis was performed using a qScript cDNA SuperMix (QuantaBio, Leuven, Belgium). ..

    cDNA Synthesis:

    Article Title: Exercise volume modulates cardiac protection in a type 2 diabetic rat model: differential effects of high- and low-volume moderate-intensity endurance exercise training on diabetic cardiomyopathy
    Article Snippet: Total RNA isolation was performed using an RNeasy Fibrous Tissue Kit (Qiagen Benelux B.V., Antwerp, Belgium), following the manufacturer’s protocol. .. RNA concentration and purity were assessed using a NanoDrop 2000 spectrophotometer (Isogen Life Science B.V., Utrecht, Netherlands). cDNA synthesis was performed using a qScript cDNA SuperMix (QuantaBio, Leuven, Belgium). ..

    Article Title: MECP2 mutations rewire human ESC fate and bias cortical lineage commitment.
    Article Snippet: Total RNA was isolated from each hiPSC line in triplicate (n = 3 biological replicates per genotype) with the RNeasy Mini Kit (Qiagen, #74106) on a QIAcube automated workstation. .. For cDNA synthesis, 500 ng of RNA were reverse-transcribed using qScript cDNA SuperMix (Quantabio, #95048-100) on a SimpliAmp thermal cycler, following the manufacturer’s protocol. ..

    SYBR Green Assay:

    Article Title: Tunable tau expression in C. elegans neurons reveals that early-AD tau phosphorylation selectively impacts behavior and mitochondrial quality control
    Article Snippet: Contaminating genomic DNA was digested using a Turbo DNA-free TM Kit (AM1907, Invitrogen, Carlsbad, CA), the RNA concentration of each sample was measured via NanoDrop (LT1022, Thermo Scientific, Waltham, MA). .. 300 ng RNA was used to create cDNA libraries from each sample using Quantabio qScript cDNA Supermix (95,048, Quantabio, Beverly, MA). qRT-PCR was run in 96-well plates using 2× Universal SYBR Green Fast qPCR Mix (RK21203, ABclonal, Woburn, MA) and the primers listed in . .. All statistical analyses were conducted using Prism 8.0 (GraphPad Software Inc., Boston, MA), with alpha-error level of p < 0.05 considered to be significant.

    Biomarker Discovery:

    Article Title: Molecular signature
    Article Snippet: .. Real Time PCR Validation For microarray validation, total RNA from the 33 pre-invasive LUSC lesions undergoing Illumina gene expression profiling was reverse transcribed using qScript® cDNA Super-Mix (Polymerase chain reaction (PCR) reagents) (Quanta Biosciences, Lutterworth, UK) according to the manufacturer's protocol. .. Real-time quantitative PCR was carried out in eight genes using the SYBR-green master mix (Applied BioSystems, Bleiswijk, Netherlands) in an Eppendorf real-time PCR Machine (Eppendorf, Stevenage, UK).

    Microarray:

    Article Title: Molecular signature
    Article Snippet: .. Real Time PCR Validation For microarray validation, total RNA from the 33 pre-invasive LUSC lesions undergoing Illumina gene expression profiling was reverse transcribed using qScript® cDNA Super-Mix (Polymerase chain reaction (PCR) reagents) (Quanta Biosciences, Lutterworth, UK) according to the manufacturer's protocol. .. Real-time quantitative PCR was carried out in eight genes using the SYBR-green master mix (Applied BioSystems, Bleiswijk, Netherlands) in an Eppendorf real-time PCR Machine (Eppendorf, Stevenage, UK).

    Gene Expression:

    Article Title: Molecular signature
    Article Snippet: .. Real Time PCR Validation For microarray validation, total RNA from the 33 pre-invasive LUSC lesions undergoing Illumina gene expression profiling was reverse transcribed using qScript® cDNA Super-Mix (Polymerase chain reaction (PCR) reagents) (Quanta Biosciences, Lutterworth, UK) according to the manufacturer's protocol. .. Real-time quantitative PCR was carried out in eight genes using the SYBR-green master mix (Applied BioSystems, Bleiswijk, Netherlands) in an Eppendorf real-time PCR Machine (Eppendorf, Stevenage, UK).

    Polymerase Chain Reaction:

    Article Title: Molecular signature
    Article Snippet: .. Real Time PCR Validation For microarray validation, total RNA from the 33 pre-invasive LUSC lesions undergoing Illumina gene expression profiling was reverse transcribed using qScript® cDNA Super-Mix (Polymerase chain reaction (PCR) reagents) (Quanta Biosciences, Lutterworth, UK) according to the manufacturer's protocol. .. Real-time quantitative PCR was carried out in eight genes using the SYBR-green master mix (Applied BioSystems, Bleiswijk, Netherlands) in an Eppendorf real-time PCR Machine (Eppendorf, Stevenage, UK).



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    Loss of NK cell IFNAR1 impairs NK cell function, may impact adaptive immune regulation of tumorigenesis and increases lung metastasis. 1 × 105 EO771.LMB cells were injected into the fourth mammary fat pad (IMFP) of controliCre and Ifnar−/−NKp46 C57BL/6 mice at day 0 and subsequent alterations to circulating and primary tumor cells assessed. Peripheral blood (PB) was taken at day 7 for FACS analysis and for intracellular staining (ICS) of monocytes was performed at endpoint. a Primary tumor weights (mg) of control (n = 7) and Ifnar−/−NKp46 mice (n = 7) mice at experimental endpoint. Flow cytometric analysis of b absolute number (cell #) of primary tumor NK cells (n = 6/group). c PB CD69+ NK (NK1.1+) cell frequency (%). Primary tumor d CD69+ (e. representative plots shown) and f IFNγ+ NK cell frequency (%) and g IFNAR1 expression, represented as MFI (n = 5/group). Flow cytometric analysis of h CD8+ T cell IFNAR1 (MFI; n = 5/group) and i. PB CD69+ CD8+ T cells, j primary tumor CD69+ CD8+ T cells, k CD8+ IFNγ+ T cells, l. PB CD69+ CD4+ T cells and m primary tumor CD4+ IFNγ+ T cells, expressed as frequency (%). n RT-qPCR of mCherry (EO771.LMB) DNA expression in the lungs post-intravenous (IV) injection of EO771.LMB cells into WT (n = 9), controliCre (n = 5) and Ifnar−/−NKp46 (n = 8) mice at day 24. Representative fluorescence imaging of mCherry (EO771.LMB) in lungs shown for each group. All PB analysis (n = 7/group) and primary tumor analysis (n = 6/group). p values * < 0.05, ** < 0.005, *** < 0.0005. **** < 0.0001 determined by Student’s t test. Errors bars, SEM

    Journal: Cancer Immunology, Immunotherapy : CII

    Article Title: Loss of type I IFN responsiveness impairs natural killer cell antitumor activity in breast cancer

    doi: 10.1007/s00262-021-02857-z

    Figure Lengend Snippet: Loss of NK cell IFNAR1 impairs NK cell function, may impact adaptive immune regulation of tumorigenesis and increases lung metastasis. 1 × 105 EO771.LMB cells were injected into the fourth mammary fat pad (IMFP) of controliCre and Ifnar−/−NKp46 C57BL/6 mice at day 0 and subsequent alterations to circulating and primary tumor cells assessed. Peripheral blood (PB) was taken at day 7 for FACS analysis and for intracellular staining (ICS) of monocytes was performed at endpoint. a Primary tumor weights (mg) of control (n = 7) and Ifnar−/−NKp46 mice (n = 7) mice at experimental endpoint. Flow cytometric analysis of b absolute number (cell #) of primary tumor NK cells (n = 6/group). c PB CD69+ NK (NK1.1+) cell frequency (%). Primary tumor d CD69+ (e. representative plots shown) and f IFNγ+ NK cell frequency (%) and g IFNAR1 expression, represented as MFI (n = 5/group). Flow cytometric analysis of h CD8+ T cell IFNAR1 (MFI; n = 5/group) and i. PB CD69+ CD8+ T cells, j primary tumor CD69+ CD8+ T cells, k CD8+ IFNγ+ T cells, l. PB CD69+ CD4+ T cells and m primary tumor CD4+ IFNγ+ T cells, expressed as frequency (%). n RT-qPCR of mCherry (EO771.LMB) DNA expression in the lungs post-intravenous (IV) injection of EO771.LMB cells into WT (n = 9), controliCre (n = 5) and Ifnar−/−NKp46 (n = 8) mice at day 24. Representative fluorescence imaging of mCherry (EO771.LMB) in lungs shown for each group. All PB analysis (n = 7/group) and primary tumor analysis (n = 6/group). p values * < 0.05, ** < 0.005, *** < 0.0005. **** < 0.0001 determined by Student’s t test. Errors bars, SEM

    Article Snippet: When required, cells were transfected with poly I:C (10 μg/ml) overnight, prior to RNA extraction. cDNA was generated using the iScript Reverse Transcriptase Supermix cDNA for RT-qPCR kit (BioRad).

    Techniques: Cell Function Assay, Injection, Staining, Expressing, Quantitative RT-PCR, IV Injection, Fluorescence, Imaging