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reverse transcriptase rt kits  (Bio-Rad)


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    Structured Review

    Bio-Rad reverse transcriptase rt kits
    Reverse Transcriptase Rt Kits, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 6616 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/iscript+reverse+transcriptase+rt+kit/IScript+C+DNA+Synthesis+Kit/pmc11225910__mmc1-7-33-39
    Average 99 stars, based on 6616 article reviews
    reverse transcriptase rt kits - by Bioz Stars, 2026-10
    99/100 stars

    Images

    Related Articles

    Reverse Transcription:

    Article Title: Targeted Intra-Atrial Delivery of GMP-Grade Human Extracellular Vesicles Prevents Inflammation-Driven Postoperative Atrial Fibrillation in Pigs
    Article Snippet: THP-1 cells were lysed in Qiazol, and RNA was extracted using the RNeasy Micro Kit (Qiagen, 1038703). .. Reverse transcription was conducted on 200 ng of the isolated RNA (iScript DNA Synthesis Kit, Bio-Rad), followed by a reverse transcription-quantitative polymerase chain reaction (Bio-Rad) according to the manufacturer protocol. ..

    Article Title: Selection of appropriate reference genes in Apoe -/- mouse brain
    Article Snippet: The concentrations and purity of RNA samples were measured using a NanoDropTM 2000/2000c Spectrophotometer (ThermoScientific). .. iScript cDNA synthesis kit was used to synthesize cDNA (1708891, BioRad, Hercules, CA, USA) and 500 ng DNase-treated RNA was used for reverse transcription. cDNA samples were diluted 4-fold, and 2 μl of the diluted cDNA was used for RT-qPCR experiments. .. LightCycler 480 SYBR Green I Master mix solution (04887352001, Roche, Mannheim, Germany) was utilized according to the manufacturer‘s instructions.

    Article Title: Generation of precise and accurate engineered circRNAs using enzymatic ligation
    Article Snippet: .. Reverse transcription for ligation junction accuracy used the iScript kit (Bio-Rad #1708 891) containing random hexamers. ..

    Isolation:

    Article Title: Targeted Intra-Atrial Delivery of GMP-Grade Human Extracellular Vesicles Prevents Inflammation-Driven Postoperative Atrial Fibrillation in Pigs
    Article Snippet: THP-1 cells were lysed in Qiazol, and RNA was extracted using the RNeasy Micro Kit (Qiagen, 1038703). .. Reverse transcription was conducted on 200 ng of the isolated RNA (iScript DNA Synthesis Kit, Bio-Rad), followed by a reverse transcription-quantitative polymerase chain reaction (Bio-Rad) according to the manufacturer protocol. ..

    DNA Synthesis:

    Article Title: Targeted Intra-Atrial Delivery of GMP-Grade Human Extracellular Vesicles Prevents Inflammation-Driven Postoperative Atrial Fibrillation in Pigs
    Article Snippet: THP-1 cells were lysed in Qiazol, and RNA was extracted using the RNeasy Micro Kit (Qiagen, 1038703). .. Reverse transcription was conducted on 200 ng of the isolated RNA (iScript DNA Synthesis Kit, Bio-Rad), followed by a reverse transcription-quantitative polymerase chain reaction (Bio-Rad) according to the manufacturer protocol. ..

    Polymerase Chain Reaction:

    Article Title: Targeted Intra-Atrial Delivery of GMP-Grade Human Extracellular Vesicles Prevents Inflammation-Driven Postoperative Atrial Fibrillation in Pigs
    Article Snippet: THP-1 cells were lysed in Qiazol, and RNA was extracted using the RNeasy Micro Kit (Qiagen, 1038703). .. Reverse transcription was conducted on 200 ng of the isolated RNA (iScript DNA Synthesis Kit, Bio-Rad), followed by a reverse transcription-quantitative polymerase chain reaction (Bio-Rad) according to the manufacturer protocol. ..

    other:

    Article Title: Human induced pluripotent stem cell-derived inner ear organoids reveal hair cell damage and plasticity after cisplatin and gentamicin exposure
    Article Snippet: RNA concentration was measured using NanoDrop One (Thermo Fisher Scientific), and samples were stored at −80°C.

    Article Title: Octopamine regulates neural circuits in the mushroom body and central complex, influencing sleep and arousal
    Article Snippet: GCamp6s was excited with a 470 nm LED light source (X-Cite turbo multiwavelength system, Excelitas Inc) and images were acquired using ORCA FLASH 4.0 V2 digital CMOS camera.

    Purification:

    Article Title: Decoupling Mechanical Confinement and Fibrotic Extracellular Matrix Signaling in Vestibular Schwannoma Using Tunable 3D Hydrogels
    Article Snippet: .. Gels were lysed in TRIzol (Invitrogen 15596026), and RNA was purified using Qiagen silica column–based kit (74104) according to Manufacturer’s protocol. cDNA was synthesized using iScript cDNA Synthesis Kit (Bio-Rad 1708890) and RT-qPCR was performed using iTaq Universal SYBR Green Supermix (Bio-Rad 1725121) on a real-time PCR system. ..

    Synthesized:

    Article Title: Decoupling Mechanical Confinement and Fibrotic Extracellular Matrix Signaling in Vestibular Schwannoma Using Tunable 3D Hydrogels
    Article Snippet: .. Gels were lysed in TRIzol (Invitrogen 15596026), and RNA was purified using Qiagen silica column–based kit (74104) according to Manufacturer’s protocol. cDNA was synthesized using iScript cDNA Synthesis Kit (Bio-Rad 1708890) and RT-qPCR was performed using iTaq Universal SYBR Green Supermix (Bio-Rad 1725121) on a real-time PCR system. ..

    cDNA Synthesis:

    Article Title: Decoupling Mechanical Confinement and Fibrotic Extracellular Matrix Signaling in Vestibular Schwannoma Using Tunable 3D Hydrogels
    Article Snippet: .. Gels were lysed in TRIzol (Invitrogen 15596026), and RNA was purified using Qiagen silica column–based kit (74104) according to Manufacturer’s protocol. cDNA was synthesized using iScript cDNA Synthesis Kit (Bio-Rad 1708890) and RT-qPCR was performed using iTaq Universal SYBR Green Supermix (Bio-Rad 1725121) on a real-time PCR system. ..

    Article Title: Integrated multiomics reveals starvation-driven keratin degradation and persistence in Fervidobacterium islandicum AW-1
    Article Snippet: CPM (7-diethylamino-3-(4’-maleimidylphenyl)-4-methyl-coumarin) , Invitrogen, MA, USA , Cat# D346. .. iScript cDNA Synthesis Kit , Bio-Rad, CA, USA , Cat# 1708890. .. 2× SsoAdvancedTM Universal SYBR Green Supermix , Bio-Rad, CA, USA , Cat# 1725271.

    Article Title: Selection of appropriate reference genes in Apoe -/- mouse brain
    Article Snippet: The concentrations and purity of RNA samples were measured using a NanoDropTM 2000/2000c Spectrophotometer (ThermoScientific). .. iScript cDNA synthesis kit was used to synthesize cDNA (1708891, BioRad, Hercules, CA, USA) and 500 ng DNase-treated RNA was used for reverse transcription. cDNA samples were diluted 4-fold, and 2 μl of the diluted cDNA was used for RT-qPCR experiments. .. LightCycler 480 SYBR Green I Master mix solution (04887352001, Roche, Mannheim, Germany) was utilized according to the manufacturer‘s instructions.

    Article Title: Heterogeneity of estrogen and β-adrenergic receptors in female human coronary artery endothelial cells
    Article Snippet: .. RNA was stored at − 80◦C for subsequent cDNA synthesis, which was carried out within 24 h using the iScript cDNA Synthesis Kit (Bio-Rad, 1708890). ..

    Quantitative RT-PCR:

    Article Title: Decoupling Mechanical Confinement and Fibrotic Extracellular Matrix Signaling in Vestibular Schwannoma Using Tunable 3D Hydrogels
    Article Snippet: .. Gels were lysed in TRIzol (Invitrogen 15596026), and RNA was purified using Qiagen silica column–based kit (74104) according to Manufacturer’s protocol. cDNA was synthesized using iScript cDNA Synthesis Kit (Bio-Rad 1708890) and RT-qPCR was performed using iTaq Universal SYBR Green Supermix (Bio-Rad 1725121) on a real-time PCR system. ..

    Article Title: Selection of appropriate reference genes in Apoe -/- mouse brain
    Article Snippet: The concentrations and purity of RNA samples were measured using a NanoDropTM 2000/2000c Spectrophotometer (ThermoScientific). .. iScript cDNA synthesis kit was used to synthesize cDNA (1708891, BioRad, Hercules, CA, USA) and 500 ng DNase-treated RNA was used for reverse transcription. cDNA samples were diluted 4-fold, and 2 μl of the diluted cDNA was used for RT-qPCR experiments. .. LightCycler 480 SYBR Green I Master mix solution (04887352001, Roche, Mannheim, Germany) was utilized according to the manufacturer‘s instructions.

    SYBR Green Assay:

    Article Title: Decoupling Mechanical Confinement and Fibrotic Extracellular Matrix Signaling in Vestibular Schwannoma Using Tunable 3D Hydrogels
    Article Snippet: .. Gels were lysed in TRIzol (Invitrogen 15596026), and RNA was purified using Qiagen silica column–based kit (74104) according to Manufacturer’s protocol. cDNA was synthesized using iScript cDNA Synthesis Kit (Bio-Rad 1708890) and RT-qPCR was performed using iTaq Universal SYBR Green Supermix (Bio-Rad 1725121) on a real-time PCR system. ..

    Real-time Polymerase Chain Reaction:

    Article Title: Decoupling Mechanical Confinement and Fibrotic Extracellular Matrix Signaling in Vestibular Schwannoma Using Tunable 3D Hydrogels
    Article Snippet: .. Gels were lysed in TRIzol (Invitrogen 15596026), and RNA was purified using Qiagen silica column–based kit (74104) according to Manufacturer’s protocol. cDNA was synthesized using iScript cDNA Synthesis Kit (Bio-Rad 1708890) and RT-qPCR was performed using iTaq Universal SYBR Green Supermix (Bio-Rad 1725121) on a real-time PCR system. ..

    Ligation:

    Article Title: Generation of precise and accurate engineered circRNAs using enzymatic ligation
    Article Snippet: .. Reverse transcription for ligation junction accuracy used the iScript kit (Bio-Rad #1708 891) containing random hexamers. ..



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    Image Search Results


    Loss of NK cell IFNAR1 impairs NK cell function, may impact adaptive immune regulation of tumorigenesis and increases lung metastasis. 1 × 105 EO771.LMB cells were injected into the fourth mammary fat pad (IMFP) of controliCre and Ifnar−/−NKp46 C57BL/6 mice at day 0 and subsequent alterations to circulating and primary tumor cells assessed. Peripheral blood (PB) was taken at day 7 for FACS analysis and for intracellular staining (ICS) of monocytes was performed at endpoint. a Primary tumor weights (mg) of control (n = 7) and Ifnar−/−NKp46 mice (n = 7) mice at experimental endpoint. Flow cytometric analysis of b absolute number (cell #) of primary tumor NK cells (n = 6/group). c PB CD69+ NK (NK1.1+) cell frequency (%). Primary tumor d CD69+ (e. representative plots shown) and f IFNγ+ NK cell frequency (%) and g IFNAR1 expression, represented as MFI (n = 5/group). Flow cytometric analysis of h CD8+ T cell IFNAR1 (MFI; n = 5/group) and i. PB CD69+ CD8+ T cells, j primary tumor CD69+ CD8+ T cells, k CD8+ IFNγ+ T cells, l. PB CD69+ CD4+ T cells and m primary tumor CD4+ IFNγ+ T cells, expressed as frequency (%). n RT-qPCR of mCherry (EO771.LMB) DNA expression in the lungs post-intravenous (IV) injection of EO771.LMB cells into WT (n = 9), controliCre (n = 5) and Ifnar−/−NKp46 (n = 8) mice at day 24. Representative fluorescence imaging of mCherry (EO771.LMB) in lungs shown for each group. All PB analysis (n = 7/group) and primary tumor analysis (n = 6/group). p values * < 0.05, ** < 0.005, *** < 0.0005. **** < 0.0001 determined by Student’s t test. Errors bars, SEM

    Journal: Cancer Immunology, Immunotherapy : CII

    Article Title: Loss of type I IFN responsiveness impairs natural killer cell antitumor activity in breast cancer

    doi: 10.1007/s00262-021-02857-z

    Figure Lengend Snippet: Loss of NK cell IFNAR1 impairs NK cell function, may impact adaptive immune regulation of tumorigenesis and increases lung metastasis. 1 × 105 EO771.LMB cells were injected into the fourth mammary fat pad (IMFP) of controliCre and Ifnar−/−NKp46 C57BL/6 mice at day 0 and subsequent alterations to circulating and primary tumor cells assessed. Peripheral blood (PB) was taken at day 7 for FACS analysis and for intracellular staining (ICS) of monocytes was performed at endpoint. a Primary tumor weights (mg) of control (n = 7) and Ifnar−/−NKp46 mice (n = 7) mice at experimental endpoint. Flow cytometric analysis of b absolute number (cell #) of primary tumor NK cells (n = 6/group). c PB CD69+ NK (NK1.1+) cell frequency (%). Primary tumor d CD69+ (e. representative plots shown) and f IFNγ+ NK cell frequency (%) and g IFNAR1 expression, represented as MFI (n = 5/group). Flow cytometric analysis of h CD8+ T cell IFNAR1 (MFI; n = 5/group) and i. PB CD69+ CD8+ T cells, j primary tumor CD69+ CD8+ T cells, k CD8+ IFNγ+ T cells, l. PB CD69+ CD4+ T cells and m primary tumor CD4+ IFNγ+ T cells, expressed as frequency (%). n RT-qPCR of mCherry (EO771.LMB) DNA expression in the lungs post-intravenous (IV) injection of EO771.LMB cells into WT (n = 9), controliCre (n = 5) and Ifnar−/−NKp46 (n = 8) mice at day 24. Representative fluorescence imaging of mCherry (EO771.LMB) in lungs shown for each group. All PB analysis (n = 7/group) and primary tumor analysis (n = 6/group). p values * < 0.05, ** < 0.005, *** < 0.0005. **** < 0.0001 determined by Student’s t test. Errors bars, SEM

    Article Snippet: When required, cells were transfected with poly I:C (10 μg/ml) overnight, prior to RNA extraction. cDNA was generated using the iScript Reverse Transcriptase Supermix cDNA for RT-qPCR kit (BioRad).

    Techniques: Cell Function Assay, Injection, Staining, Expressing, Quantitative RT-PCR, IV Injection, Fluorescence, Imaging