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iscript one‐step reverse‐transcriptase polymerase chain reaction (rt‐pcr) kit  (Bio-Rad)


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    Bio-Rad iscript one‐step reverse‐transcriptase polymerase chain reaction (rt‐pcr) kit
    Iscript One‐Step Reverse‐Transcriptase Polymerase Chain Reaction (Rt‐Pcr) Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/iscript+reverse+transcriptase+rt+kit/iscript+one+step+reverse+transcriptase+polymerase+chain+reaction++rt+pcr++kit/pmc05442794-71-30-38
    Average 90 stars, based on 1 article reviews
    iscript one‐step reverse‐transcriptase polymerase chain reaction (rt‐pcr) kit - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Isolation:

    Article Title: Electromechanical Conditioning of Adult Progenitor Cells Improves Recovery of Cardiac Function After Myocardial Infarction
    Article Snippet: Total RNA was isolated from cardiac and subcutaneous ATDPCs by using the AllPrep RNA/Protein Kit (Qiagen, Hilden, Germany, http://www.qiagen.com ). cDNA was synthesized by using random hexamers (Qiagen) and the iScript One‐Step reverse‐transcriptase polymerase chain reaction (RT‐PCR) kit (Bio‐Rad, Hercules, CA, http://www.bio‐rad.com ) according to the manufacturer's protocol. cDNA was preamplified with the TaqMan PreAmp Master Mix Kit (Applied Biosystems, Foster City, CA, http://www.appliedbiosystems.com ) and then diluted 1:5 with RNase‐free water.

    Synthesized:

    Article Title: Electromechanical Conditioning of Adult Progenitor Cells Improves Recovery of Cardiac Function After Myocardial Infarction
    Article Snippet: Total RNA was isolated from cardiac and subcutaneous ATDPCs by using the AllPrep RNA/Protein Kit (Qiagen, Hilden, Germany, http://www.qiagen.com ). cDNA was synthesized by using random hexamers (Qiagen) and the iScript One‐Step reverse‐transcriptase polymerase chain reaction (RT‐PCR) kit (Bio‐Rad, Hercules, CA, http://www.bio‐rad.com ) according to the manufacturer's protocol. cDNA was preamplified with the TaqMan PreAmp Master Mix Kit (Applied Biosystems, Foster City, CA, http://www.appliedbiosystems.com ) and then diluted 1:5 with RNase‐free water.

    Polymerase Chain Reaction:

    Article Title: Electromechanical Conditioning of Adult Progenitor Cells Improves Recovery of Cardiac Function After Myocardial Infarction
    Article Snippet: Total RNA was isolated from cardiac and subcutaneous ATDPCs by using the AllPrep RNA/Protein Kit (Qiagen, Hilden, Germany, http://www.qiagen.com ). cDNA was synthesized by using random hexamers (Qiagen) and the iScript One‐Step reverse‐transcriptase polymerase chain reaction (RT‐PCR) kit (Bio‐Rad, Hercules, CA, http://www.bio‐rad.com ) according to the manufacturer's protocol. cDNA was preamplified with the TaqMan PreAmp Master Mix Kit (Applied Biosystems, Foster City, CA, http://www.appliedbiosystems.com ) and then diluted 1:5 with RNase‐free water.



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    Image Search Results


    Loss of NK cell IFNAR1 impairs NK cell function, may impact adaptive immune regulation of tumorigenesis and increases lung metastasis. 1 × 105 EO771.LMB cells were injected into the fourth mammary fat pad (IMFP) of controliCre and Ifnar−/−NKp46 C57BL/6 mice at day 0 and subsequent alterations to circulating and primary tumor cells assessed. Peripheral blood (PB) was taken at day 7 for FACS analysis and for intracellular staining (ICS) of monocytes was performed at endpoint. a Primary tumor weights (mg) of control (n = 7) and Ifnar−/−NKp46 mice (n = 7) mice at experimental endpoint. Flow cytometric analysis of b absolute number (cell #) of primary tumor NK cells (n = 6/group). c PB CD69+ NK (NK1.1+) cell frequency (%). Primary tumor d CD69+ (e. representative plots shown) and f IFNγ+ NK cell frequency (%) and g IFNAR1 expression, represented as MFI (n = 5/group). Flow cytometric analysis of h CD8+ T cell IFNAR1 (MFI; n = 5/group) and i. PB CD69+ CD8+ T cells, j primary tumor CD69+ CD8+ T cells, k CD8+ IFNγ+ T cells, l. PB CD69+ CD4+ T cells and m primary tumor CD4+ IFNγ+ T cells, expressed as frequency (%). n RT-qPCR of mCherry (EO771.LMB) DNA expression in the lungs post-intravenous (IV) injection of EO771.LMB cells into WT (n = 9), controliCre (n = 5) and Ifnar−/−NKp46 (n = 8) mice at day 24. Representative fluorescence imaging of mCherry (EO771.LMB) in lungs shown for each group. All PB analysis (n = 7/group) and primary tumor analysis (n = 6/group). p values * < 0.05, ** < 0.005, *** < 0.0005. **** < 0.0001 determined by Student’s t test. Errors bars, SEM

    Journal: Cancer Immunology, Immunotherapy : CII

    Article Title: Loss of type I IFN responsiveness impairs natural killer cell antitumor activity in breast cancer

    doi: 10.1007/s00262-021-02857-z

    Figure Lengend Snippet: Loss of NK cell IFNAR1 impairs NK cell function, may impact adaptive immune regulation of tumorigenesis and increases lung metastasis. 1 × 105 EO771.LMB cells were injected into the fourth mammary fat pad (IMFP) of controliCre and Ifnar−/−NKp46 C57BL/6 mice at day 0 and subsequent alterations to circulating and primary tumor cells assessed. Peripheral blood (PB) was taken at day 7 for FACS analysis and for intracellular staining (ICS) of monocytes was performed at endpoint. a Primary tumor weights (mg) of control (n = 7) and Ifnar−/−NKp46 mice (n = 7) mice at experimental endpoint. Flow cytometric analysis of b absolute number (cell #) of primary tumor NK cells (n = 6/group). c PB CD69+ NK (NK1.1+) cell frequency (%). Primary tumor d CD69+ (e. representative plots shown) and f IFNγ+ NK cell frequency (%) and g IFNAR1 expression, represented as MFI (n = 5/group). Flow cytometric analysis of h CD8+ T cell IFNAR1 (MFI; n = 5/group) and i. PB CD69+ CD8+ T cells, j primary tumor CD69+ CD8+ T cells, k CD8+ IFNγ+ T cells, l. PB CD69+ CD4+ T cells and m primary tumor CD4+ IFNγ+ T cells, expressed as frequency (%). n RT-qPCR of mCherry (EO771.LMB) DNA expression in the lungs post-intravenous (IV) injection of EO771.LMB cells into WT (n = 9), controliCre (n = 5) and Ifnar−/−NKp46 (n = 8) mice at day 24. Representative fluorescence imaging of mCherry (EO771.LMB) in lungs shown for each group. All PB analysis (n = 7/group) and primary tumor analysis (n = 6/group). p values * < 0.05, ** < 0.005, *** < 0.0005. **** < 0.0001 determined by Student’s t test. Errors bars, SEM

    Article Snippet: When required, cells were transfected with poly I:C (10 μg/ml) overnight, prior to RNA extraction. cDNA was generated using the iScript Reverse Transcriptase Supermix cDNA for RT-qPCR kit (BioRad).

    Techniques: Cell Function Assay, Injection, Staining, Expressing, Quantitative RT-PCR, IV Injection, Fluorescence, Imaging