Review



qscript cdna supermix kit  (Quanta Biosciences)


Bioz Verified Symbol Quanta Biosciences is a verified supplier
Bioz Manufacturer Symbol Quanta Biosciences manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 97
      Buy from Supplier

    Structured Review

    Quanta Biosciences qscript cdna supermix kit
    Qscript Cdna Supermix Kit, supplied by Quanta Biosciences, used in various techniques. Bioz Stars score: 97/100, based on 4421 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/iscript+reverse+transcriptase+kit/qScript+cDNA+SuperMix/pm37075982-165-21-25
    Average 97 stars, based on 4421 article reviews
    qscript cdna supermix kit - by Bioz Stars, 2026-11
    97/100 stars

    Images

    Related Articles

    Reverse Transcription:

    Article Title: Time-dependent transcriptomic response to scrapie exposure in ovine MSC-derived neuron-like cells.
    Article Snippet: .. Reverse transcription was carried out using 200 ng of total RNA with qScript cDNA SuperMix (Quanta Biosciences), following the manufacturer’s instructions. .. The resulting cDNA was diluted 1:5 in water, and gene expression was quantified by RT-qPCR using Fast SYBR Green Master Mix (Applied Biosystems, Thermo Fisher Scientific) on a StepOne Plus Real-Time PCR instrument (Applied Biosystems).

    Article Title: Genetic dissection of DNA damage response pathways reveals divergent modulation of PD-1 blockade response in colorectal cancer
    Article Snippet: RNA was extracted using a RNeasy kit (Qiagen) per the manufacture’s protocol. .. Reverse transcription was performed using qScript cDNA SuperMix (Quantabio). ..

    Article Title: PLD1-driven oncogenic secretion creates a therapeutic vulnerability in chromosome 3q-amplified NSCLC.
    Article Snippet: To isolate the total RNA from cells, we utilized the RNeasy Mini Kit (Qiagen). .. Reverse transcription was carried out using the qScript cDNA superMix (Quanta Biosciences). .. Genomic DNA was extracted from cells using the DNeasy Blood and Tissue Kits (Qiagen).

    cDNA Synthesis:

    Article Title: Mitochondrial stress induces transcription of cytosolic and mitochondrial protein synthesis components via NHR-180.
    Article Snippet: .. Total RNA was extracted from frozen worm pellets using Trizol reagent, and 500 ng RNA was used for cDNA synthesis with qScriptTM cDNA SuperMix (QuantaBio). .. qPCR was performed using iQTM SYBR Green Supermix (Bio-Rad Laboratories).

    Article Title: Identification of TREK channels in mouse intracardiac ganglion neurons
    Article Snippet: .. J Physiol 0.0 TREK channels in intracardiac ganglion neurons 19 Table A1. (Continued) Product Company/distributor Reference Rabbit anti-KCNK2 Alomone Labs APC-047 Rabbit anti-KCNK10 Alomone Labs APC-055 Mouse anti-NeuN Tocris NBP1-92693SS Anti-rabbit AlexaFluor555 Invitrogen A31572 Anti-mouse AlexaFluor488 Thermo Fisher Scientific A-11 001 Prolong Antifade Gold with DAPI Invitrogen P36941 Patch clamp NaCl Fisher Scientific 7647-14-5 KCl Sigma-Aldrich 7447-40-7 MgCl2 EMSURE 7791-18-6 CaCl2 EMSURE 10 035-04-8 D-Glucose Sigma-Aldrich G5767 HEPES Sigma-Aldrich H3375 Tris (hydroxymethyl)-amino-methane Sigma-Aldrich 77-86-1 Potassium acetate Merck K24483720 EGTA Sigma-Aldrich E4378 Amphotericin-B Sigma-Aldrich A4888 NaOH Panreac 131 687 HCl Sigma-Aldrich 100 317 DMSO Tocris 3176 TTX Tocris 4368-28-9 CdCl2 Sigma-Aldrich 202 908 CsCl Sigma-Aldrich C3139 TEA Sigma-Aldrich T0886 Riluzole Sigma-Aldrich R-116 4-Aminopyridine Sigma-Aldrich A7840-3 Apamin Tocris 1652 BL-1249 Tocris 3797 Paxilline Tocris 2006 Clemizole hydrochloride Tocris 5371 Zncl2 Hellobio 21 127-3 Bacl2 Sigma-Aldrich B-0750 Fluoxetine Sigma-Aldrich F132 Spadin Sigma-Aldrich SML1500 RNA extraction, cDNA synthesis and qPCR RNeasy Micro Kit Qiagen 74 004 qScript cDNA SuperMix Quantabio 95 048-025 Rbfox3 forward (5-CAC CAC TCT CTT GTC CGT TTG C-3) and reverse (5- GGC TGA GCA TAT CTG TAA GCT GC -3) STAB VIDA Tnnt2 forward (5-GCT ACA GAC TCT GAT CGA GGC T-3) and reverse (5-GCT CAT TGC GAA TAC GCT GCT G-3) STAB VIDA Kcnk2 forward (5-CAC GGT GTT CAA GGC ATT GGA G-3) and reverse (5-TCC CTG CGT TTA TTG CTG CCA C-3) STAB VIDA Kcnk4 forward (5-GTC ACT CTC ACC ACT GTA GGC T-3) and reverse (5-TGG TGA GCA CTG AGG CGA AGT A-3) STAB VIDA Kcnk10 forward (5-CTG TCC TCA GTA TGA TCG GAG AC-3) and reverse (5-CAG CAG TGA CAT TAG CCT TCC AC-3) STAB VIDA GAPDH forward (5-CAT CAC TGC CAC CCA GAA GAC TG-3) and reverse (5-ATG CCA GTG AGC TTC CCG TTC AG-3) STAB VIDA AceQ Universal SYBR qPCR Master Mix NeoBiotech Q511-02 The Author(s) ..

    Synthesized:

    Article Title: Dietary Arthrospira platensis modulates autophagy-related markers in breast muscle of heat-stressed broilers and chicken primary myoblasts.
    Article Snippet: The concentrations and purity of RNA were accessed using a Take3 Microvolume with a BioTek Synergy HT Multimode Microplate Reader (BioTek, Winooski, VT). .. Complementary DNA was synthesized from 1 μg of total RNA using qScript cDNA SuperMix (Quanta Biosciences, Gaithersburg, MD). .. The relative expression of target genes was quantified by 7500 Real-Time PCR System (Applied Biosystems, Waltham, MA) using 2 × Universal SYBR Green Fast qPCR Mix (ABclonal, Woburn, MA).

    Article Title: iPSC neurodegenerative disease initiative isogenic CAG repeat iPSC line for Huntington's disease.
    Article Snippet: RNA purity and concentration were verified using a NanoDrop spectrophotometer by monitoring A260/280 and A260/230 ratios. .. cDNA was synthesized from 1 μg of total RNA using the QScript cDNA SuperMix (QuantaBio 101414-106) on a Bio-Rad T100 Thermal Cycler. ..

    Patch Clamp:

    Article Title: Identification of TREK channels in mouse intracardiac ganglion neurons
    Article Snippet: .. J Physiol 0.0 TREK channels in intracardiac ganglion neurons 19 Table A1. (Continued) Product Company/distributor Reference Rabbit anti-KCNK2 Alomone Labs APC-047 Rabbit anti-KCNK10 Alomone Labs APC-055 Mouse anti-NeuN Tocris NBP1-92693SS Anti-rabbit AlexaFluor555 Invitrogen A31572 Anti-mouse AlexaFluor488 Thermo Fisher Scientific A-11 001 Prolong Antifade Gold with DAPI Invitrogen P36941 Patch clamp NaCl Fisher Scientific 7647-14-5 KCl Sigma-Aldrich 7447-40-7 MgCl2 EMSURE 7791-18-6 CaCl2 EMSURE 10 035-04-8 D-Glucose Sigma-Aldrich G5767 HEPES Sigma-Aldrich H3375 Tris (hydroxymethyl)-amino-methane Sigma-Aldrich 77-86-1 Potassium acetate Merck K24483720 EGTA Sigma-Aldrich E4378 Amphotericin-B Sigma-Aldrich A4888 NaOH Panreac 131 687 HCl Sigma-Aldrich 100 317 DMSO Tocris 3176 TTX Tocris 4368-28-9 CdCl2 Sigma-Aldrich 202 908 CsCl Sigma-Aldrich C3139 TEA Sigma-Aldrich T0886 Riluzole Sigma-Aldrich R-116 4-Aminopyridine Sigma-Aldrich A7840-3 Apamin Tocris 1652 BL-1249 Tocris 3797 Paxilline Tocris 2006 Clemizole hydrochloride Tocris 5371 Zncl2 Hellobio 21 127-3 Bacl2 Sigma-Aldrich B-0750 Fluoxetine Sigma-Aldrich F132 Spadin Sigma-Aldrich SML1500 RNA extraction, cDNA synthesis and qPCR RNeasy Micro Kit Qiagen 74 004 qScript cDNA SuperMix Quantabio 95 048-025 Rbfox3 forward (5-CAC CAC TCT CTT GTC CGT TTG C-3) and reverse (5- GGC TGA GCA TAT CTG TAA GCT GC -3) STAB VIDA Tnnt2 forward (5-GCT ACA GAC TCT GAT CGA GGC T-3) and reverse (5-GCT CAT TGC GAA TAC GCT GCT G-3) STAB VIDA Kcnk2 forward (5-CAC GGT GTT CAA GGC ATT GGA G-3) and reverse (5-TCC CTG CGT TTA TTG CTG CCA C-3) STAB VIDA Kcnk4 forward (5-GTC ACT CTC ACC ACT GTA GGC T-3) and reverse (5-TGG TGA GCA CTG AGG CGA AGT A-3) STAB VIDA Kcnk10 forward (5-CTG TCC TCA GTA TGA TCG GAG AC-3) and reverse (5-CAG CAG TGA CAT TAG CCT TCC AC-3) STAB VIDA GAPDH forward (5-CAT CAC TGC CAC CCA GAA GAC TG-3) and reverse (5-ATG CCA GTG AGC TTC CCG TTC AG-3) STAB VIDA AceQ Universal SYBR qPCR Master Mix NeoBiotech Q511-02 The Author(s) ..

    RNA Extraction:

    Article Title: Identification of TREK channels in mouse intracardiac ganglion neurons
    Article Snippet: .. J Physiol 0.0 TREK channels in intracardiac ganglion neurons 19 Table A1. (Continued) Product Company/distributor Reference Rabbit anti-KCNK2 Alomone Labs APC-047 Rabbit anti-KCNK10 Alomone Labs APC-055 Mouse anti-NeuN Tocris NBP1-92693SS Anti-rabbit AlexaFluor555 Invitrogen A31572 Anti-mouse AlexaFluor488 Thermo Fisher Scientific A-11 001 Prolong Antifade Gold with DAPI Invitrogen P36941 Patch clamp NaCl Fisher Scientific 7647-14-5 KCl Sigma-Aldrich 7447-40-7 MgCl2 EMSURE 7791-18-6 CaCl2 EMSURE 10 035-04-8 D-Glucose Sigma-Aldrich G5767 HEPES Sigma-Aldrich H3375 Tris (hydroxymethyl)-amino-methane Sigma-Aldrich 77-86-1 Potassium acetate Merck K24483720 EGTA Sigma-Aldrich E4378 Amphotericin-B Sigma-Aldrich A4888 NaOH Panreac 131 687 HCl Sigma-Aldrich 100 317 DMSO Tocris 3176 TTX Tocris 4368-28-9 CdCl2 Sigma-Aldrich 202 908 CsCl Sigma-Aldrich C3139 TEA Sigma-Aldrich T0886 Riluzole Sigma-Aldrich R-116 4-Aminopyridine Sigma-Aldrich A7840-3 Apamin Tocris 1652 BL-1249 Tocris 3797 Paxilline Tocris 2006 Clemizole hydrochloride Tocris 5371 Zncl2 Hellobio 21 127-3 Bacl2 Sigma-Aldrich B-0750 Fluoxetine Sigma-Aldrich F132 Spadin Sigma-Aldrich SML1500 RNA extraction, cDNA synthesis and qPCR RNeasy Micro Kit Qiagen 74 004 qScript cDNA SuperMix Quantabio 95 048-025 Rbfox3 forward (5-CAC CAC TCT CTT GTC CGT TTG C-3) and reverse (5- GGC TGA GCA TAT CTG TAA GCT GC -3) STAB VIDA Tnnt2 forward (5-GCT ACA GAC TCT GAT CGA GGC T-3) and reverse (5-GCT CAT TGC GAA TAC GCT GCT G-3) STAB VIDA Kcnk2 forward (5-CAC GGT GTT CAA GGC ATT GGA G-3) and reverse (5-TCC CTG CGT TTA TTG CTG CCA C-3) STAB VIDA Kcnk4 forward (5-GTC ACT CTC ACC ACT GTA GGC T-3) and reverse (5-TGG TGA GCA CTG AGG CGA AGT A-3) STAB VIDA Kcnk10 forward (5-CTG TCC TCA GTA TGA TCG GAG AC-3) and reverse (5-CAG CAG TGA CAT TAG CCT TCC AC-3) STAB VIDA GAPDH forward (5-CAT CAC TGC CAC CCA GAA GAC TG-3) and reverse (5-ATG CCA GTG AGC TTC CCG TTC AG-3) STAB VIDA AceQ Universal SYBR qPCR Master Mix NeoBiotech Q511-02 The Author(s) ..

    Real-time Polymerase Chain Reaction:

    Article Title: Identification of TREK channels in mouse intracardiac ganglion neurons
    Article Snippet: .. J Physiol 0.0 TREK channels in intracardiac ganglion neurons 19 Table A1. (Continued) Product Company/distributor Reference Rabbit anti-KCNK2 Alomone Labs APC-047 Rabbit anti-KCNK10 Alomone Labs APC-055 Mouse anti-NeuN Tocris NBP1-92693SS Anti-rabbit AlexaFluor555 Invitrogen A31572 Anti-mouse AlexaFluor488 Thermo Fisher Scientific A-11 001 Prolong Antifade Gold with DAPI Invitrogen P36941 Patch clamp NaCl Fisher Scientific 7647-14-5 KCl Sigma-Aldrich 7447-40-7 MgCl2 EMSURE 7791-18-6 CaCl2 EMSURE 10 035-04-8 D-Glucose Sigma-Aldrich G5767 HEPES Sigma-Aldrich H3375 Tris (hydroxymethyl)-amino-methane Sigma-Aldrich 77-86-1 Potassium acetate Merck K24483720 EGTA Sigma-Aldrich E4378 Amphotericin-B Sigma-Aldrich A4888 NaOH Panreac 131 687 HCl Sigma-Aldrich 100 317 DMSO Tocris 3176 TTX Tocris 4368-28-9 CdCl2 Sigma-Aldrich 202 908 CsCl Sigma-Aldrich C3139 TEA Sigma-Aldrich T0886 Riluzole Sigma-Aldrich R-116 4-Aminopyridine Sigma-Aldrich A7840-3 Apamin Tocris 1652 BL-1249 Tocris 3797 Paxilline Tocris 2006 Clemizole hydrochloride Tocris 5371 Zncl2 Hellobio 21 127-3 Bacl2 Sigma-Aldrich B-0750 Fluoxetine Sigma-Aldrich F132 Spadin Sigma-Aldrich SML1500 RNA extraction, cDNA synthesis and qPCR RNeasy Micro Kit Qiagen 74 004 qScript cDNA SuperMix Quantabio 95 048-025 Rbfox3 forward (5-CAC CAC TCT CTT GTC CGT TTG C-3) and reverse (5- GGC TGA GCA TAT CTG TAA GCT GC -3) STAB VIDA Tnnt2 forward (5-GCT ACA GAC TCT GAT CGA GGC T-3) and reverse (5-GCT CAT TGC GAA TAC GCT GCT G-3) STAB VIDA Kcnk2 forward (5-CAC GGT GTT CAA GGC ATT GGA G-3) and reverse (5-TCC CTG CGT TTA TTG CTG CCA C-3) STAB VIDA Kcnk4 forward (5-GTC ACT CTC ACC ACT GTA GGC T-3) and reverse (5-TGG TGA GCA CTG AGG CGA AGT A-3) STAB VIDA Kcnk10 forward (5-CTG TCC TCA GTA TGA TCG GAG AC-3) and reverse (5-CAG CAG TGA CAT TAG CCT TCC AC-3) STAB VIDA GAPDH forward (5-CAT CAC TGC CAC CCA GAA GAC TG-3) and reverse (5-ATG CCA GTG AGC TTC CCG TTC AG-3) STAB VIDA AceQ Universal SYBR qPCR Master Mix NeoBiotech Q511-02 The Author(s) ..

    CTG Assay:

    Article Title: Identification of TREK channels in mouse intracardiac ganglion neurons
    Article Snippet: .. J Physiol 0.0 TREK channels in intracardiac ganglion neurons 19 Table A1. (Continued) Product Company/distributor Reference Rabbit anti-KCNK2 Alomone Labs APC-047 Rabbit anti-KCNK10 Alomone Labs APC-055 Mouse anti-NeuN Tocris NBP1-92693SS Anti-rabbit AlexaFluor555 Invitrogen A31572 Anti-mouse AlexaFluor488 Thermo Fisher Scientific A-11 001 Prolong Antifade Gold with DAPI Invitrogen P36941 Patch clamp NaCl Fisher Scientific 7647-14-5 KCl Sigma-Aldrich 7447-40-7 MgCl2 EMSURE 7791-18-6 CaCl2 EMSURE 10 035-04-8 D-Glucose Sigma-Aldrich G5767 HEPES Sigma-Aldrich H3375 Tris (hydroxymethyl)-amino-methane Sigma-Aldrich 77-86-1 Potassium acetate Merck K24483720 EGTA Sigma-Aldrich E4378 Amphotericin-B Sigma-Aldrich A4888 NaOH Panreac 131 687 HCl Sigma-Aldrich 100 317 DMSO Tocris 3176 TTX Tocris 4368-28-9 CdCl2 Sigma-Aldrich 202 908 CsCl Sigma-Aldrich C3139 TEA Sigma-Aldrich T0886 Riluzole Sigma-Aldrich R-116 4-Aminopyridine Sigma-Aldrich A7840-3 Apamin Tocris 1652 BL-1249 Tocris 3797 Paxilline Tocris 2006 Clemizole hydrochloride Tocris 5371 Zncl2 Hellobio 21 127-3 Bacl2 Sigma-Aldrich B-0750 Fluoxetine Sigma-Aldrich F132 Spadin Sigma-Aldrich SML1500 RNA extraction, cDNA synthesis and qPCR RNeasy Micro Kit Qiagen 74 004 qScript cDNA SuperMix Quantabio 95 048-025 Rbfox3 forward (5-CAC CAC TCT CTT GTC CGT TTG C-3) and reverse (5- GGC TGA GCA TAT CTG TAA GCT GC -3) STAB VIDA Tnnt2 forward (5-GCT ACA GAC TCT GAT CGA GGC T-3) and reverse (5-GCT CAT TGC GAA TAC GCT GCT G-3) STAB VIDA Kcnk2 forward (5-CAC GGT GTT CAA GGC ATT GGA G-3) and reverse (5-TCC CTG CGT TTA TTG CTG CCA C-3) STAB VIDA Kcnk4 forward (5-GTC ACT CTC ACC ACT GTA GGC T-3) and reverse (5-TGG TGA GCA CTG AGG CGA AGT A-3) STAB VIDA Kcnk10 forward (5-CTG TCC TCA GTA TGA TCG GAG AC-3) and reverse (5-CAG CAG TGA CAT TAG CCT TCC AC-3) STAB VIDA GAPDH forward (5-CAT CAC TGC CAC CCA GAA GAC TG-3) and reverse (5-ATG CCA GTG AGC TTC CCG TTC AG-3) STAB VIDA AceQ Universal SYBR qPCR Master Mix NeoBiotech Q511-02 The Author(s) ..

    Gas Chromatography:

    Article Title: Identification of TREK channels in mouse intracardiac ganglion neurons
    Article Snippet: .. J Physiol 0.0 TREK channels in intracardiac ganglion neurons 19 Table A1. (Continued) Product Company/distributor Reference Rabbit anti-KCNK2 Alomone Labs APC-047 Rabbit anti-KCNK10 Alomone Labs APC-055 Mouse anti-NeuN Tocris NBP1-92693SS Anti-rabbit AlexaFluor555 Invitrogen A31572 Anti-mouse AlexaFluor488 Thermo Fisher Scientific A-11 001 Prolong Antifade Gold with DAPI Invitrogen P36941 Patch clamp NaCl Fisher Scientific 7647-14-5 KCl Sigma-Aldrich 7447-40-7 MgCl2 EMSURE 7791-18-6 CaCl2 EMSURE 10 035-04-8 D-Glucose Sigma-Aldrich G5767 HEPES Sigma-Aldrich H3375 Tris (hydroxymethyl)-amino-methane Sigma-Aldrich 77-86-1 Potassium acetate Merck K24483720 EGTA Sigma-Aldrich E4378 Amphotericin-B Sigma-Aldrich A4888 NaOH Panreac 131 687 HCl Sigma-Aldrich 100 317 DMSO Tocris 3176 TTX Tocris 4368-28-9 CdCl2 Sigma-Aldrich 202 908 CsCl Sigma-Aldrich C3139 TEA Sigma-Aldrich T0886 Riluzole Sigma-Aldrich R-116 4-Aminopyridine Sigma-Aldrich A7840-3 Apamin Tocris 1652 BL-1249 Tocris 3797 Paxilline Tocris 2006 Clemizole hydrochloride Tocris 5371 Zncl2 Hellobio 21 127-3 Bacl2 Sigma-Aldrich B-0750 Fluoxetine Sigma-Aldrich F132 Spadin Sigma-Aldrich SML1500 RNA extraction, cDNA synthesis and qPCR RNeasy Micro Kit Qiagen 74 004 qScript cDNA SuperMix Quantabio 95 048-025 Rbfox3 forward (5-CAC CAC TCT CTT GTC CGT TTG C-3) and reverse (5- GGC TGA GCA TAT CTG TAA GCT GC -3) STAB VIDA Tnnt2 forward (5-GCT ACA GAC TCT GAT CGA GGC T-3) and reverse (5-GCT CAT TGC GAA TAC GCT GCT G-3) STAB VIDA Kcnk2 forward (5-CAC GGT GTT CAA GGC ATT GGA G-3) and reverse (5-TCC CTG CGT TTA TTG CTG CCA C-3) STAB VIDA Kcnk4 forward (5-GTC ACT CTC ACC ACT GTA GGC T-3) and reverse (5-TGG TGA GCA CTG AGG CGA AGT A-3) STAB VIDA Kcnk10 forward (5-CTG TCC TCA GTA TGA TCG GAG AC-3) and reverse (5-CAG CAG TGA CAT TAG CCT TCC AC-3) STAB VIDA GAPDH forward (5-CAT CAC TGC CAC CCA GAA GAC TG-3) and reverse (5-ATG CCA GTG AGC TTC CCG TTC AG-3) STAB VIDA AceQ Universal SYBR qPCR Master Mix NeoBiotech Q511-02 The Author(s) ..

    Chloramphenicol Acetyltransferase Assay:

    Article Title: Identification of TREK channels in mouse intracardiac ganglion neurons
    Article Snippet: .. J Physiol 0.0 TREK channels in intracardiac ganglion neurons 19 Table A1. (Continued) Product Company/distributor Reference Rabbit anti-KCNK2 Alomone Labs APC-047 Rabbit anti-KCNK10 Alomone Labs APC-055 Mouse anti-NeuN Tocris NBP1-92693SS Anti-rabbit AlexaFluor555 Invitrogen A31572 Anti-mouse AlexaFluor488 Thermo Fisher Scientific A-11 001 Prolong Antifade Gold with DAPI Invitrogen P36941 Patch clamp NaCl Fisher Scientific 7647-14-5 KCl Sigma-Aldrich 7447-40-7 MgCl2 EMSURE 7791-18-6 CaCl2 EMSURE 10 035-04-8 D-Glucose Sigma-Aldrich G5767 HEPES Sigma-Aldrich H3375 Tris (hydroxymethyl)-amino-methane Sigma-Aldrich 77-86-1 Potassium acetate Merck K24483720 EGTA Sigma-Aldrich E4378 Amphotericin-B Sigma-Aldrich A4888 NaOH Panreac 131 687 HCl Sigma-Aldrich 100 317 DMSO Tocris 3176 TTX Tocris 4368-28-9 CdCl2 Sigma-Aldrich 202 908 CsCl Sigma-Aldrich C3139 TEA Sigma-Aldrich T0886 Riluzole Sigma-Aldrich R-116 4-Aminopyridine Sigma-Aldrich A7840-3 Apamin Tocris 1652 BL-1249 Tocris 3797 Paxilline Tocris 2006 Clemizole hydrochloride Tocris 5371 Zncl2 Hellobio 21 127-3 Bacl2 Sigma-Aldrich B-0750 Fluoxetine Sigma-Aldrich F132 Spadin Sigma-Aldrich SML1500 RNA extraction, cDNA synthesis and qPCR RNeasy Micro Kit Qiagen 74 004 qScript cDNA SuperMix Quantabio 95 048-025 Rbfox3 forward (5-CAC CAC TCT CTT GTC CGT TTG C-3) and reverse (5- GGC TGA GCA TAT CTG TAA GCT GC -3) STAB VIDA Tnnt2 forward (5-GCT ACA GAC TCT GAT CGA GGC T-3) and reverse (5-GCT CAT TGC GAA TAC GCT GCT G-3) STAB VIDA Kcnk2 forward (5-CAC GGT GTT CAA GGC ATT GGA G-3) and reverse (5-TCC CTG CGT TTA TTG CTG CCA C-3) STAB VIDA Kcnk4 forward (5-GTC ACT CTC ACC ACT GTA GGC T-3) and reverse (5-TGG TGA GCA CTG AGG CGA AGT A-3) STAB VIDA Kcnk10 forward (5-CTG TCC TCA GTA TGA TCG GAG AC-3) and reverse (5-CAG CAG TGA CAT TAG CCT TCC AC-3) STAB VIDA GAPDH forward (5-CAT CAC TGC CAC CCA GAA GAC TG-3) and reverse (5-ATG CCA GTG AGC TTC CCG TTC AG-3) STAB VIDA AceQ Universal SYBR qPCR Master Mix NeoBiotech Q511-02 The Author(s) ..

    Cellular Antioxidant Activity Assay:

    Article Title: Identification of TREK channels in mouse intracardiac ganglion neurons
    Article Snippet: .. J Physiol 0.0 TREK channels in intracardiac ganglion neurons 19 Table A1. (Continued) Product Company/distributor Reference Rabbit anti-KCNK2 Alomone Labs APC-047 Rabbit anti-KCNK10 Alomone Labs APC-055 Mouse anti-NeuN Tocris NBP1-92693SS Anti-rabbit AlexaFluor555 Invitrogen A31572 Anti-mouse AlexaFluor488 Thermo Fisher Scientific A-11 001 Prolong Antifade Gold with DAPI Invitrogen P36941 Patch clamp NaCl Fisher Scientific 7647-14-5 KCl Sigma-Aldrich 7447-40-7 MgCl2 EMSURE 7791-18-6 CaCl2 EMSURE 10 035-04-8 D-Glucose Sigma-Aldrich G5767 HEPES Sigma-Aldrich H3375 Tris (hydroxymethyl)-amino-methane Sigma-Aldrich 77-86-1 Potassium acetate Merck K24483720 EGTA Sigma-Aldrich E4378 Amphotericin-B Sigma-Aldrich A4888 NaOH Panreac 131 687 HCl Sigma-Aldrich 100 317 DMSO Tocris 3176 TTX Tocris 4368-28-9 CdCl2 Sigma-Aldrich 202 908 CsCl Sigma-Aldrich C3139 TEA Sigma-Aldrich T0886 Riluzole Sigma-Aldrich R-116 4-Aminopyridine Sigma-Aldrich A7840-3 Apamin Tocris 1652 BL-1249 Tocris 3797 Paxilline Tocris 2006 Clemizole hydrochloride Tocris 5371 Zncl2 Hellobio 21 127-3 Bacl2 Sigma-Aldrich B-0750 Fluoxetine Sigma-Aldrich F132 Spadin Sigma-Aldrich SML1500 RNA extraction, cDNA synthesis and qPCR RNeasy Micro Kit Qiagen 74 004 qScript cDNA SuperMix Quantabio 95 048-025 Rbfox3 forward (5-CAC CAC TCT CTT GTC CGT TTG C-3) and reverse (5- GGC TGA GCA TAT CTG TAA GCT GC -3) STAB VIDA Tnnt2 forward (5-GCT ACA GAC TCT GAT CGA GGC T-3) and reverse (5-GCT CAT TGC GAA TAC GCT GCT G-3) STAB VIDA Kcnk2 forward (5-CAC GGT GTT CAA GGC ATT GGA G-3) and reverse (5-TCC CTG CGT TTA TTG CTG CCA C-3) STAB VIDA Kcnk4 forward (5-GTC ACT CTC ACC ACT GTA GGC T-3) and reverse (5-TGG TGA GCA CTG AGG CGA AGT A-3) STAB VIDA Kcnk10 forward (5-CTG TCC TCA GTA TGA TCG GAG AC-3) and reverse (5-CAG CAG TGA CAT TAG CCT TCC AC-3) STAB VIDA GAPDH forward (5-CAT CAC TGC CAC CCA GAA GAC TG-3) and reverse (5-ATG CCA GTG AGC TTC CCG TTC AG-3) STAB VIDA AceQ Universal SYBR qPCR Master Mix NeoBiotech Q511-02 The Author(s) ..

    Activated Clotting Time Assay:

    Article Title: Identification of TREK channels in mouse intracardiac ganglion neurons
    Article Snippet: .. J Physiol 0.0 TREK channels in intracardiac ganglion neurons 19 Table A1. (Continued) Product Company/distributor Reference Rabbit anti-KCNK2 Alomone Labs APC-047 Rabbit anti-KCNK10 Alomone Labs APC-055 Mouse anti-NeuN Tocris NBP1-92693SS Anti-rabbit AlexaFluor555 Invitrogen A31572 Anti-mouse AlexaFluor488 Thermo Fisher Scientific A-11 001 Prolong Antifade Gold with DAPI Invitrogen P36941 Patch clamp NaCl Fisher Scientific 7647-14-5 KCl Sigma-Aldrich 7447-40-7 MgCl2 EMSURE 7791-18-6 CaCl2 EMSURE 10 035-04-8 D-Glucose Sigma-Aldrich G5767 HEPES Sigma-Aldrich H3375 Tris (hydroxymethyl)-amino-methane Sigma-Aldrich 77-86-1 Potassium acetate Merck K24483720 EGTA Sigma-Aldrich E4378 Amphotericin-B Sigma-Aldrich A4888 NaOH Panreac 131 687 HCl Sigma-Aldrich 100 317 DMSO Tocris 3176 TTX Tocris 4368-28-9 CdCl2 Sigma-Aldrich 202 908 CsCl Sigma-Aldrich C3139 TEA Sigma-Aldrich T0886 Riluzole Sigma-Aldrich R-116 4-Aminopyridine Sigma-Aldrich A7840-3 Apamin Tocris 1652 BL-1249 Tocris 3797 Paxilline Tocris 2006 Clemizole hydrochloride Tocris 5371 Zncl2 Hellobio 21 127-3 Bacl2 Sigma-Aldrich B-0750 Fluoxetine Sigma-Aldrich F132 Spadin Sigma-Aldrich SML1500 RNA extraction, cDNA synthesis and qPCR RNeasy Micro Kit Qiagen 74 004 qScript cDNA SuperMix Quantabio 95 048-025 Rbfox3 forward (5-CAC CAC TCT CTT GTC CGT TTG C-3) and reverse (5- GGC TGA GCA TAT CTG TAA GCT GC -3) STAB VIDA Tnnt2 forward (5-GCT ACA GAC TCT GAT CGA GGC T-3) and reverse (5-GCT CAT TGC GAA TAC GCT GCT G-3) STAB VIDA Kcnk2 forward (5-CAC GGT GTT CAA GGC ATT GGA G-3) and reverse (5-TCC CTG CGT TTA TTG CTG CCA C-3) STAB VIDA Kcnk4 forward (5-GTC ACT CTC ACC ACT GTA GGC T-3) and reverse (5-TGG TGA GCA CTG AGG CGA AGT A-3) STAB VIDA Kcnk10 forward (5-CTG TCC TCA GTA TGA TCG GAG AC-3) and reverse (5-CAG CAG TGA CAT TAG CCT TCC AC-3) STAB VIDA GAPDH forward (5-CAT CAC TGC CAC CCA GAA GAC TG-3) and reverse (5-ATG CCA GTG AGC TTC CCG TTC AG-3) STAB VIDA AceQ Universal SYBR qPCR Master Mix NeoBiotech Q511-02 The Author(s) ..

    Knock-Out:

    Article Title: TM6SF2 acts at ER membrane contact sites to enable RAB7-dependent endolysosomal VLDL secretion and maintain Golgi structure.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-APOB clone C1.4 Santa Cruz Biotechnology Cat# sc-13538; RRID:AB_626690 Mouse monoclonal anti-Rab7 (E9O7E) Cell Signaling Technology Cat# 95746; RRID:AB_2800252 Rabbit monoclonal anti-Rab7 (D95F2) Cell Signaling Technology Cat# 9367; RRID:AB_1904103 Mouse monoclonal anti-GFP clone B2 Santa Cruz Biotechnology Cat# sc-9996; RRID:AB_627695 Mouse monoclonal anti-FLAG-M2 Sigma-Aldrich Cat# F3165; RRID:AB_259529 Mouse monoclonal anti-HA-Tag (6E2) Cell Signaling Technology Cat# 2367; RRID:AB_10691311 Rabbit monoclonal anti-HA-Tag (C29F4) Cell Signaling Technology Cat# 3724; RRID:AB_1549585 Rabbit monoclonal anti-GST-Tag (91G1) Cell Signaling Technology Cat# 2625; RRID:AB_490796 Rabbit monoclonal anti-β-Actin (13E5) Cell Signaling Technology Cat# 4970; RRID:AB_2223172 Rabbit polyclonal anti-VAPA (VAP-33) Bethyl Laboratories Cat# A304-366A; RRID:AB_2620561 Rabbit polyclonal anti-VAPB Bethyl Laboratories Cat# A302-894A; RRID:AB_10663774 Rabbit monoclonal anti-STING (D2P2F) Cell Signaling Technology Cat# 13647; RRID:AB_2732796 Rabbit monoclonal anti-IRF-3 (D83B9) Cell Signaling Technology Cat# 4302; RRID:AB_1904036 Rabbit monoclonal anti-phospho-IRF-3 (Ser386) (E7J8G) Cell Signaling Technology Cat# 37829; RRID:AB_2799121 Rabbit monoclonal anti-TBK1/NAK (D1B4) Cell Signaling Technology Cat# 3504; RRID:AB_2255663 Rabbit monoclonal anti-phospho-TBK1/NAK (Ser172) (D52C2) Cell Signaling Technology Cat# 5483; RRID:AB_10693472 Rabbit monoclonal anti-NF-κB p65 (D14E12) Cell Signaling Technology Cat# 8242; RRID:AB_10859369 Rabbit monoclonal anti-phosphoNF-κB p65 (Ser536) (93H1) Cell Signaling Technology Cat# 3033; RRID:AB_331284 Rabbit monoclonal anti-GM130 (D6B1) Cell Signaling Technology Cat# 12480; RRID:AB_2797933 Sheep polyclonal anti-TGN46 Bio-Rad Laboratories Cat# AHP500GT; RRID:AB_2203291 Rabbit monoclonal anti-LAMP1 (D2D11) Cell Signaling Technology Cat# 9091; RRID:AB_2687579 Rabbit polyclonal anti-RAB5 Cell Signaling Technology Cat# 3547; RRID:AB_2300649 Rabbit monoclonal anti-EEA1 (C45B10) Cell Signaling Technology Cat# 3288; RRID:AB_2096811 Rabbit polyclonal anti-SEC31A Proteintech Cat# 17913-1-AP; RRID:AB_2186378 Rabbit monoclonal anti-LC3B (D11) Cell Signaling Technology Cat# 3868; RRID:AB_2137707 Goat poly polyclonal anti-human Albumin Bethyl Laboratories Cat# A80-229A; RRID:AB_67018 Mouse monoclonal anti-HNF4A Abcam Cat# ab41898; RRID:AB_732976 Rabbit polyclonal anti-PtdIns(4)P IgM Echelon Bioscience Cat# Z-P004; RRID:AB_11127796 Bacterial and virus strains NEB 5-alpha Competent E. coli (High Efficiency) New England BioLabs Cat# C2987H BL21 (DE3) E. coli New England BioLabs Cat# C2527H Biological samples Liver tissue samples from patients with MASH UCSF Department of Pathology N/A Chemicals, peptides, and recombinant proteins Recombinant human insulin GeminiBio Cat# 800–112P–100 Recombinant human FGFb Peprotech Cat# 100-18B Recombinant human BMP4 Peprotech Cat# 120-05ET Activin A StemCell Technologies Cat# 78001 Recombinant human HGF Peprotech Cat# 100–39 Recombinant human Oncostatin M Peprotech Cat# 300–10 CHIR99021 StemCell Technologies Cat# 72054 (Continued on next page) 18 Cell Reports 45, 118116, October 27, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Dexamethasone Sigma-Aldrich Cat# D4902 1-Thioglycerol Sigma-Aldrich Cat# M6145 PI103 MedChemExpress Cat# HY-10115 Sodium butyrate TCI Chemicals Cat# S0519 Y-27632 Tocris Cat# 1254 Non-essential amino acids (NEAA) Thermo Fisher Scientific Cat# 11140050 Biotin Sigma-Aldrich Cat# B4639 ADU-S100 MedChemExpress Cat# HY-12885 Oleic acid Sigma-Aldrich Cat# O1383 Polybrene Sigma-Aldrich Cat# H9268 DMEM medium Thermo Fisher Scientific Cat# 10569044 RPMI 1640 medium Thermo Fisher Scientific Cat# 11875093 mTeSR1 medium StemCell Technologies Cat# 85850 Hepatocyte Culture Medium (HCM) Lonza Cat# 185389 Opti-MEM medium Thermo Fisher Scientific Cat# 31985070 KnockOut DMEM medium Thermo Fisher Scientific Cat# 10829018 KnockOut Serum Replacement (KSR) Thermo Fisher Scientific Cat# 10828028 Gem21 without insulin GeminiBio Cat# 400–962 GlutaMAX Thermo Fisher Scientific Cat# 35050061 Cultrex Reduced Growth Factor Basement Membrane Extract (RGF-BME) R&D Systems Cat# 3433–001 Type I Collagen Corning Cat# 354249 IPTG Thermo Fisher Scientific Cat# 15529019 Halt protease inhibitor cocktail Thermo Fisher Scientific Cat# 78429 Halt phosphatase inhibitor cocktail Thermo Fisher Scientific Cat# 78420 Dynabeads protein G Thermo Fisher Scientific Cat# 10003D Glutathione Agarose Thermo Fisher Scientific Cat# 21516 LipidTOX Green Thermo Fisher Scientific Cat# H34475 Lipofectamine 3000 Thermo Fisher Scientific Cat# L3000001 Lipofectamine RNAiMAX Thermo Fisher Scientific Cat# 13778150 Critical commercial assays Triglycerides Liquid Stable Reagent Thermo Fisher Scientific Cat# TR22421 PureLink RNA Mini Kit Thermo Fisher Scientific Cat# 12183025 qScript cDNA SuperMix Quantabio Cat# 95048 SYBR Green PCR Master Mix Thermo Fisher Scientific Cat# 4309155 PureLink Genomic DNA Mini Kit Thermo Fisher Scientific Cat# K182001 HiFi DNA assembly kit New England BioLabs Cat# E2621 Pierce GST Protein Interaction Pull-Down Kit Thermo Fisher Scientific Cat# 21516 MycoAlert Mycoplasma Detection Kit Lonza Cat# LT07-318 Experimental models: Cell lines HEK293T ATCC Cat# CRL-3216 HepG2 ATCC Cat# HB-8065 Huh7 Japanese Collection of Research Bioresources Cell Bank Cat# JCRB0403 Healthy donor iPSC line CIRM iPSC repository at Fujifilm Cellular Dynamics N/A Same healthy donor iPSC line with TM6SF2 E167K gene edit Synthego N/A (Continued on next page) Cell Reports 45, 118116, October 27, 2026 19 .. REAGENT or RESOURCE SOURCE IDENTIFIER iPSC lines from patients with MASH CIRM iPSC repository at Fujifilm Cellular Dynamics N/A Primary human hepatocytes UCSF Liver Center N/A Oligonucleotides TM6SF2 E167K sgRNA: 5′GCAAAUACAGCUCCGAGAUC3′ Synthego N/A TM6SF2 E167K donor template: 5′ATGCCCTCTCTCCTGCACCATGGAAGGCAAATA CAGCTCCAAGATTAGGCCTGCCTTCTTCCTCACCA TCCCCTACCTGCT3′ Synthego N/A TM6SF2 KO sgRNA: 5′CTACGCGCTCAACCACGTCT3′ IDT N/A TM6SF2 DsiRNA 13.1: Sense: 5′AAGGAAGAGAUACCGGAAUUUUGGA3′ Antisense: 5′UCCAAAAUUCCGGUAUCUCUUCCUUCU3′ IDT N/A TM6SF2 DsiRNA 13.2: Sense: 5′CAGUAAAAACCCUUUCAAUUUCCAA3′ Antisense: 5′UUGGAAAUUGAAAGGGUUUUUACUGAA3′ IDT N/A TM6SF2 DsiRNA 13.3: Sense: 5′GACCUGGCCCUUGUCAUAUAUCUCA3′ Antisense: 5′UGAGAUAUAUGACAAGGGCCAGGUCAG3′ IDT N/A RAB7 DsiRNA 13.1: Sense: 5′AACCAGUAUGUGAAUAAGAAAUUCA3′ Antisense: 5′UGAAUUUCUUAUUCACAUACUGGUUCA3′ IDT N/A RAB7 DsiRNA 13.2: Sense: 5′GUGCUACAGCAAAAACAACAUUCCC3′ Antisense: 5′GGGAAUGUUGUUUUUGCUGUAGCACCA3′ IDT N/A RAB7 DsiRNA 13.3: Sense: 5′CCGUUAGAUCAGCAUUCUACUACAA3′ Antisense: 5′UUGUAGUAGAAUGCUGAUCUAACGGGA3′ IDT N/A TM6SF2 qPCR primers: Fwd: 5′TGGTGGTGCTTGATTGCCC3′ Rev: 5′CATGTGCGAGAACTGTGCCT3′ IDT N/A RAB7 qPCR primers: Fwd: 5′GTGTTGCTGAAGGTTATCATCCT3′ Rev: 5′GCTCCTATTGTGGCTTTGTACTG3′ IDT N/A CXCL10 qPCR primers: Fwd: 5′GTGGCATTCAAGGAGTACCTC3′ Rev: 5′TGATGGCCTTCGATTCTGGATT3′ IDT N/A ALB qPCR primers: Fwd: 5′AAGCTGCCTGCCTGTTGCCAA3′ Rev: 5′GGCGAGCTACTGCCCATGCTT3′ IDT N/A HNF4A qPCR primers: Fwd: 5′CCGCTTTGGAGAGCTGCT3′ Rev: 5′GGTTGTCAATCTTGGCCATGC3′ IDT N/A APOB qPCR primers: Fwd: 5′TGCTCCACTCACTTTACCGTC3′ Rev: 5′TAGCGTCCAGTGTGTACTGAC3′ IDT N/A GAPDH qPCR primers: Fwd: 5′TGCACCACCAACTGCTTAGC3′ Rev: 5′GGCATGGACTGTGGTCATGAG3′ IDT N/A Recombinant DNA mCherry This study N/A mCherry-TM6SF2 This study N/A (Continued on next page) 20 Cell Reports 45, 118116, October 27, 2026

    Membrane:

    Article Title: TM6SF2 acts at ER membrane contact sites to enable RAB7-dependent endolysosomal VLDL secretion and maintain Golgi structure.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-APOB clone C1.4 Santa Cruz Biotechnology Cat# sc-13538; RRID:AB_626690 Mouse monoclonal anti-Rab7 (E9O7E) Cell Signaling Technology Cat# 95746; RRID:AB_2800252 Rabbit monoclonal anti-Rab7 (D95F2) Cell Signaling Technology Cat# 9367; RRID:AB_1904103 Mouse monoclonal anti-GFP clone B2 Santa Cruz Biotechnology Cat# sc-9996; RRID:AB_627695 Mouse monoclonal anti-FLAG-M2 Sigma-Aldrich Cat# F3165; RRID:AB_259529 Mouse monoclonal anti-HA-Tag (6E2) Cell Signaling Technology Cat# 2367; RRID:AB_10691311 Rabbit monoclonal anti-HA-Tag (C29F4) Cell Signaling Technology Cat# 3724; RRID:AB_1549585 Rabbit monoclonal anti-GST-Tag (91G1) Cell Signaling Technology Cat# 2625; RRID:AB_490796 Rabbit monoclonal anti-β-Actin (13E5) Cell Signaling Technology Cat# 4970; RRID:AB_2223172 Rabbit polyclonal anti-VAPA (VAP-33) Bethyl Laboratories Cat# A304-366A; RRID:AB_2620561 Rabbit polyclonal anti-VAPB Bethyl Laboratories Cat# A302-894A; RRID:AB_10663774 Rabbit monoclonal anti-STING (D2P2F) Cell Signaling Technology Cat# 13647; RRID:AB_2732796 Rabbit monoclonal anti-IRF-3 (D83B9) Cell Signaling Technology Cat# 4302; RRID:AB_1904036 Rabbit monoclonal anti-phospho-IRF-3 (Ser386) (E7J8G) Cell Signaling Technology Cat# 37829; RRID:AB_2799121 Rabbit monoclonal anti-TBK1/NAK (D1B4) Cell Signaling Technology Cat# 3504; RRID:AB_2255663 Rabbit monoclonal anti-phospho-TBK1/NAK (Ser172) (D52C2) Cell Signaling Technology Cat# 5483; RRID:AB_10693472 Rabbit monoclonal anti-NF-κB p65 (D14E12) Cell Signaling Technology Cat# 8242; RRID:AB_10859369 Rabbit monoclonal anti-phosphoNF-κB p65 (Ser536) (93H1) Cell Signaling Technology Cat# 3033; RRID:AB_331284 Rabbit monoclonal anti-GM130 (D6B1) Cell Signaling Technology Cat# 12480; RRID:AB_2797933 Sheep polyclonal anti-TGN46 Bio-Rad Laboratories Cat# AHP500GT; RRID:AB_2203291 Rabbit monoclonal anti-LAMP1 (D2D11) Cell Signaling Technology Cat# 9091; RRID:AB_2687579 Rabbit polyclonal anti-RAB5 Cell Signaling Technology Cat# 3547; RRID:AB_2300649 Rabbit monoclonal anti-EEA1 (C45B10) Cell Signaling Technology Cat# 3288; RRID:AB_2096811 Rabbit polyclonal anti-SEC31A Proteintech Cat# 17913-1-AP; RRID:AB_2186378 Rabbit monoclonal anti-LC3B (D11) Cell Signaling Technology Cat# 3868; RRID:AB_2137707 Goat poly polyclonal anti-human Albumin Bethyl Laboratories Cat# A80-229A; RRID:AB_67018 Mouse monoclonal anti-HNF4A Abcam Cat# ab41898; RRID:AB_732976 Rabbit polyclonal anti-PtdIns(4)P IgM Echelon Bioscience Cat# Z-P004; RRID:AB_11127796 Bacterial and virus strains NEB 5-alpha Competent E. coli (High Efficiency) New England BioLabs Cat# C2987H BL21 (DE3) E. coli New England BioLabs Cat# C2527H Biological samples Liver tissue samples from patients with MASH UCSF Department of Pathology N/A Chemicals, peptides, and recombinant proteins Recombinant human insulin GeminiBio Cat# 800–112P–100 Recombinant human FGFb Peprotech Cat# 100-18B Recombinant human BMP4 Peprotech Cat# 120-05ET Activin A StemCell Technologies Cat# 78001 Recombinant human HGF Peprotech Cat# 100–39 Recombinant human Oncostatin M Peprotech Cat# 300–10 CHIR99021 StemCell Technologies Cat# 72054 (Continued on next page) 18 Cell Reports 45, 118116, October 27, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Dexamethasone Sigma-Aldrich Cat# D4902 1-Thioglycerol Sigma-Aldrich Cat# M6145 PI103 MedChemExpress Cat# HY-10115 Sodium butyrate TCI Chemicals Cat# S0519 Y-27632 Tocris Cat# 1254 Non-essential amino acids (NEAA) Thermo Fisher Scientific Cat# 11140050 Biotin Sigma-Aldrich Cat# B4639 ADU-S100 MedChemExpress Cat# HY-12885 Oleic acid Sigma-Aldrich Cat# O1383 Polybrene Sigma-Aldrich Cat# H9268 DMEM medium Thermo Fisher Scientific Cat# 10569044 RPMI 1640 medium Thermo Fisher Scientific Cat# 11875093 mTeSR1 medium StemCell Technologies Cat# 85850 Hepatocyte Culture Medium (HCM) Lonza Cat# 185389 Opti-MEM medium Thermo Fisher Scientific Cat# 31985070 KnockOut DMEM medium Thermo Fisher Scientific Cat# 10829018 KnockOut Serum Replacement (KSR) Thermo Fisher Scientific Cat# 10828028 Gem21 without insulin GeminiBio Cat# 400–962 GlutaMAX Thermo Fisher Scientific Cat# 35050061 Cultrex Reduced Growth Factor Basement Membrane Extract (RGF-BME) R&D Systems Cat# 3433–001 Type I Collagen Corning Cat# 354249 IPTG Thermo Fisher Scientific Cat# 15529019 Halt protease inhibitor cocktail Thermo Fisher Scientific Cat# 78429 Halt phosphatase inhibitor cocktail Thermo Fisher Scientific Cat# 78420 Dynabeads protein G Thermo Fisher Scientific Cat# 10003D Glutathione Agarose Thermo Fisher Scientific Cat# 21516 LipidTOX Green Thermo Fisher Scientific Cat# H34475 Lipofectamine 3000 Thermo Fisher Scientific Cat# L3000001 Lipofectamine RNAiMAX Thermo Fisher Scientific Cat# 13778150 Critical commercial assays Triglycerides Liquid Stable Reagent Thermo Fisher Scientific Cat# TR22421 PureLink RNA Mini Kit Thermo Fisher Scientific Cat# 12183025 qScript cDNA SuperMix Quantabio Cat# 95048 SYBR Green PCR Master Mix Thermo Fisher Scientific Cat# 4309155 PureLink Genomic DNA Mini Kit Thermo Fisher Scientific Cat# K182001 HiFi DNA assembly kit New England BioLabs Cat# E2621 Pierce GST Protein Interaction Pull-Down Kit Thermo Fisher Scientific Cat# 21516 MycoAlert Mycoplasma Detection Kit Lonza Cat# LT07-318 Experimental models: Cell lines HEK293T ATCC Cat# CRL-3216 HepG2 ATCC Cat# HB-8065 Huh7 Japanese Collection of Research Bioresources Cell Bank Cat# JCRB0403 Healthy donor iPSC line CIRM iPSC repository at Fujifilm Cellular Dynamics N/A Same healthy donor iPSC line with TM6SF2 E167K gene edit Synthego N/A (Continued on next page) Cell Reports 45, 118116, October 27, 2026 19 .. REAGENT or RESOURCE SOURCE IDENTIFIER iPSC lines from patients with MASH CIRM iPSC repository at Fujifilm Cellular Dynamics N/A Primary human hepatocytes UCSF Liver Center N/A Oligonucleotides TM6SF2 E167K sgRNA: 5′GCAAAUACAGCUCCGAGAUC3′ Synthego N/A TM6SF2 E167K donor template: 5′ATGCCCTCTCTCCTGCACCATGGAAGGCAAATA CAGCTCCAAGATTAGGCCTGCCTTCTTCCTCACCA TCCCCTACCTGCT3′ Synthego N/A TM6SF2 KO sgRNA: 5′CTACGCGCTCAACCACGTCT3′ IDT N/A TM6SF2 DsiRNA 13.1: Sense: 5′AAGGAAGAGAUACCGGAAUUUUGGA3′ Antisense: 5′UCCAAAAUUCCGGUAUCUCUUCCUUCU3′ IDT N/A TM6SF2 DsiRNA 13.2: Sense: 5′CAGUAAAAACCCUUUCAAUUUCCAA3′ Antisense: 5′UUGGAAAUUGAAAGGGUUUUUACUGAA3′ IDT N/A TM6SF2 DsiRNA 13.3: Sense: 5′GACCUGGCCCUUGUCAUAUAUCUCA3′ Antisense: 5′UGAGAUAUAUGACAAGGGCCAGGUCAG3′ IDT N/A RAB7 DsiRNA 13.1: Sense: 5′AACCAGUAUGUGAAUAAGAAAUUCA3′ Antisense: 5′UGAAUUUCUUAUUCACAUACUGGUUCA3′ IDT N/A RAB7 DsiRNA 13.2: Sense: 5′GUGCUACAGCAAAAACAACAUUCCC3′ Antisense: 5′GGGAAUGUUGUUUUUGCUGUAGCACCA3′ IDT N/A RAB7 DsiRNA 13.3: Sense: 5′CCGUUAGAUCAGCAUUCUACUACAA3′ Antisense: 5′UUGUAGUAGAAUGCUGAUCUAACGGGA3′ IDT N/A TM6SF2 qPCR primers: Fwd: 5′TGGTGGTGCTTGATTGCCC3′ Rev: 5′CATGTGCGAGAACTGTGCCT3′ IDT N/A RAB7 qPCR primers: Fwd: 5′GTGTTGCTGAAGGTTATCATCCT3′ Rev: 5′GCTCCTATTGTGGCTTTGTACTG3′ IDT N/A CXCL10 qPCR primers: Fwd: 5′GTGGCATTCAAGGAGTACCTC3′ Rev: 5′TGATGGCCTTCGATTCTGGATT3′ IDT N/A ALB qPCR primers: Fwd: 5′AAGCTGCCTGCCTGTTGCCAA3′ Rev: 5′GGCGAGCTACTGCCCATGCTT3′ IDT N/A HNF4A qPCR primers: Fwd: 5′CCGCTTTGGAGAGCTGCT3′ Rev: 5′GGTTGTCAATCTTGGCCATGC3′ IDT N/A APOB qPCR primers: Fwd: 5′TGCTCCACTCACTTTACCGTC3′ Rev: 5′TAGCGTCCAGTGTGTACTGAC3′ IDT N/A GAPDH qPCR primers: Fwd: 5′TGCACCACCAACTGCTTAGC3′ Rev: 5′GGCATGGACTGTGGTCATGAG3′ IDT N/A Recombinant DNA mCherry This study N/A mCherry-TM6SF2 This study N/A (Continued on next page) 20 Cell Reports 45, 118116, October 27, 2026

    Protease Inhibitor:

    Article Title: TM6SF2 acts at ER membrane contact sites to enable RAB7-dependent endolysosomal VLDL secretion and maintain Golgi structure.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-APOB clone C1.4 Santa Cruz Biotechnology Cat# sc-13538; RRID:AB_626690 Mouse monoclonal anti-Rab7 (E9O7E) Cell Signaling Technology Cat# 95746; RRID:AB_2800252 Rabbit monoclonal anti-Rab7 (D95F2) Cell Signaling Technology Cat# 9367; RRID:AB_1904103 Mouse monoclonal anti-GFP clone B2 Santa Cruz Biotechnology Cat# sc-9996; RRID:AB_627695 Mouse monoclonal anti-FLAG-M2 Sigma-Aldrich Cat# F3165; RRID:AB_259529 Mouse monoclonal anti-HA-Tag (6E2) Cell Signaling Technology Cat# 2367; RRID:AB_10691311 Rabbit monoclonal anti-HA-Tag (C29F4) Cell Signaling Technology Cat# 3724; RRID:AB_1549585 Rabbit monoclonal anti-GST-Tag (91G1) Cell Signaling Technology Cat# 2625; RRID:AB_490796 Rabbit monoclonal anti-β-Actin (13E5) Cell Signaling Technology Cat# 4970; RRID:AB_2223172 Rabbit polyclonal anti-VAPA (VAP-33) Bethyl Laboratories Cat# A304-366A; RRID:AB_2620561 Rabbit polyclonal anti-VAPB Bethyl Laboratories Cat# A302-894A; RRID:AB_10663774 Rabbit monoclonal anti-STING (D2P2F) Cell Signaling Technology Cat# 13647; RRID:AB_2732796 Rabbit monoclonal anti-IRF-3 (D83B9) Cell Signaling Technology Cat# 4302; RRID:AB_1904036 Rabbit monoclonal anti-phospho-IRF-3 (Ser386) (E7J8G) Cell Signaling Technology Cat# 37829; RRID:AB_2799121 Rabbit monoclonal anti-TBK1/NAK (D1B4) Cell Signaling Technology Cat# 3504; RRID:AB_2255663 Rabbit monoclonal anti-phospho-TBK1/NAK (Ser172) (D52C2) Cell Signaling Technology Cat# 5483; RRID:AB_10693472 Rabbit monoclonal anti-NF-κB p65 (D14E12) Cell Signaling Technology Cat# 8242; RRID:AB_10859369 Rabbit monoclonal anti-phosphoNF-κB p65 (Ser536) (93H1) Cell Signaling Technology Cat# 3033; RRID:AB_331284 Rabbit monoclonal anti-GM130 (D6B1) Cell Signaling Technology Cat# 12480; RRID:AB_2797933 Sheep polyclonal anti-TGN46 Bio-Rad Laboratories Cat# AHP500GT; RRID:AB_2203291 Rabbit monoclonal anti-LAMP1 (D2D11) Cell Signaling Technology Cat# 9091; RRID:AB_2687579 Rabbit polyclonal anti-RAB5 Cell Signaling Technology Cat# 3547; RRID:AB_2300649 Rabbit monoclonal anti-EEA1 (C45B10) Cell Signaling Technology Cat# 3288; RRID:AB_2096811 Rabbit polyclonal anti-SEC31A Proteintech Cat# 17913-1-AP; RRID:AB_2186378 Rabbit monoclonal anti-LC3B (D11) Cell Signaling Technology Cat# 3868; RRID:AB_2137707 Goat poly polyclonal anti-human Albumin Bethyl Laboratories Cat# A80-229A; RRID:AB_67018 Mouse monoclonal anti-HNF4A Abcam Cat# ab41898; RRID:AB_732976 Rabbit polyclonal anti-PtdIns(4)P IgM Echelon Bioscience Cat# Z-P004; RRID:AB_11127796 Bacterial and virus strains NEB 5-alpha Competent E. coli (High Efficiency) New England BioLabs Cat# C2987H BL21 (DE3) E. coli New England BioLabs Cat# C2527H Biological samples Liver tissue samples from patients with MASH UCSF Department of Pathology N/A Chemicals, peptides, and recombinant proteins Recombinant human insulin GeminiBio Cat# 800–112P–100 Recombinant human FGFb Peprotech Cat# 100-18B Recombinant human BMP4 Peprotech Cat# 120-05ET Activin A StemCell Technologies Cat# 78001 Recombinant human HGF Peprotech Cat# 100–39 Recombinant human Oncostatin M Peprotech Cat# 300–10 CHIR99021 StemCell Technologies Cat# 72054 (Continued on next page) 18 Cell Reports 45, 118116, October 27, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Dexamethasone Sigma-Aldrich Cat# D4902 1-Thioglycerol Sigma-Aldrich Cat# M6145 PI103 MedChemExpress Cat# HY-10115 Sodium butyrate TCI Chemicals Cat# S0519 Y-27632 Tocris Cat# 1254 Non-essential amino acids (NEAA) Thermo Fisher Scientific Cat# 11140050 Biotin Sigma-Aldrich Cat# B4639 ADU-S100 MedChemExpress Cat# HY-12885 Oleic acid Sigma-Aldrich Cat# O1383 Polybrene Sigma-Aldrich Cat# H9268 DMEM medium Thermo Fisher Scientific Cat# 10569044 RPMI 1640 medium Thermo Fisher Scientific Cat# 11875093 mTeSR1 medium StemCell Technologies Cat# 85850 Hepatocyte Culture Medium (HCM) Lonza Cat# 185389 Opti-MEM medium Thermo Fisher Scientific Cat# 31985070 KnockOut DMEM medium Thermo Fisher Scientific Cat# 10829018 KnockOut Serum Replacement (KSR) Thermo Fisher Scientific Cat# 10828028 Gem21 without insulin GeminiBio Cat# 400–962 GlutaMAX Thermo Fisher Scientific Cat# 35050061 Cultrex Reduced Growth Factor Basement Membrane Extract (RGF-BME) R&D Systems Cat# 3433–001 Type I Collagen Corning Cat# 354249 IPTG Thermo Fisher Scientific Cat# 15529019 Halt protease inhibitor cocktail Thermo Fisher Scientific Cat# 78429 Halt phosphatase inhibitor cocktail Thermo Fisher Scientific Cat# 78420 Dynabeads protein G Thermo Fisher Scientific Cat# 10003D Glutathione Agarose Thermo Fisher Scientific Cat# 21516 LipidTOX Green Thermo Fisher Scientific Cat# H34475 Lipofectamine 3000 Thermo Fisher Scientific Cat# L3000001 Lipofectamine RNAiMAX Thermo Fisher Scientific Cat# 13778150 Critical commercial assays Triglycerides Liquid Stable Reagent Thermo Fisher Scientific Cat# TR22421 PureLink RNA Mini Kit Thermo Fisher Scientific Cat# 12183025 qScript cDNA SuperMix Quantabio Cat# 95048 SYBR Green PCR Master Mix Thermo Fisher Scientific Cat# 4309155 PureLink Genomic DNA Mini Kit Thermo Fisher Scientific Cat# K182001 HiFi DNA assembly kit New England BioLabs Cat# E2621 Pierce GST Protein Interaction Pull-Down Kit Thermo Fisher Scientific Cat# 21516 MycoAlert Mycoplasma Detection Kit Lonza Cat# LT07-318 Experimental models: Cell lines HEK293T ATCC Cat# CRL-3216 HepG2 ATCC Cat# HB-8065 Huh7 Japanese Collection of Research Bioresources Cell Bank Cat# JCRB0403 Healthy donor iPSC line CIRM iPSC repository at Fujifilm Cellular Dynamics N/A Same healthy donor iPSC line with TM6SF2 E167K gene edit Synthego N/A (Continued on next page) Cell Reports 45, 118116, October 27, 2026 19 .. REAGENT or RESOURCE SOURCE IDENTIFIER iPSC lines from patients with MASH CIRM iPSC repository at Fujifilm Cellular Dynamics N/A Primary human hepatocytes UCSF Liver Center N/A Oligonucleotides TM6SF2 E167K sgRNA: 5′GCAAAUACAGCUCCGAGAUC3′ Synthego N/A TM6SF2 E167K donor template: 5′ATGCCCTCTCTCCTGCACCATGGAAGGCAAATA CAGCTCCAAGATTAGGCCTGCCTTCTTCCTCACCA TCCCCTACCTGCT3′ Synthego N/A TM6SF2 KO sgRNA: 5′CTACGCGCTCAACCACGTCT3′ IDT N/A TM6SF2 DsiRNA 13.1: Sense: 5′AAGGAAGAGAUACCGGAAUUUUGGA3′ Antisense: 5′UCCAAAAUUCCGGUAUCUCUUCCUUCU3′ IDT N/A TM6SF2 DsiRNA 13.2: Sense: 5′CAGUAAAAACCCUUUCAAUUUCCAA3′ Antisense: 5′UUGGAAAUUGAAAGGGUUUUUACUGAA3′ IDT N/A TM6SF2 DsiRNA 13.3: Sense: 5′GACCUGGCCCUUGUCAUAUAUCUCA3′ Antisense: 5′UGAGAUAUAUGACAAGGGCCAGGUCAG3′ IDT N/A RAB7 DsiRNA 13.1: Sense: 5′AACCAGUAUGUGAAUAAGAAAUUCA3′ Antisense: 5′UGAAUUUCUUAUUCACAUACUGGUUCA3′ IDT N/A RAB7 DsiRNA 13.2: Sense: 5′GUGCUACAGCAAAAACAACAUUCCC3′ Antisense: 5′GGGAAUGUUGUUUUUGCUGUAGCACCA3′ IDT N/A RAB7 DsiRNA 13.3: Sense: 5′CCGUUAGAUCAGCAUUCUACUACAA3′ Antisense: 5′UUGUAGUAGAAUGCUGAUCUAACGGGA3′ IDT N/A TM6SF2 qPCR primers: Fwd: 5′TGGTGGTGCTTGATTGCCC3′ Rev: 5′CATGTGCGAGAACTGTGCCT3′ IDT N/A RAB7 qPCR primers: Fwd: 5′GTGTTGCTGAAGGTTATCATCCT3′ Rev: 5′GCTCCTATTGTGGCTTTGTACTG3′ IDT N/A CXCL10 qPCR primers: Fwd: 5′GTGGCATTCAAGGAGTACCTC3′ Rev: 5′TGATGGCCTTCGATTCTGGATT3′ IDT N/A ALB qPCR primers: Fwd: 5′AAGCTGCCTGCCTGTTGCCAA3′ Rev: 5′GGCGAGCTACTGCCCATGCTT3′ IDT N/A HNF4A qPCR primers: Fwd: 5′CCGCTTTGGAGAGCTGCT3′ Rev: 5′GGTTGTCAATCTTGGCCATGC3′ IDT N/A APOB qPCR primers: Fwd: 5′TGCTCCACTCACTTTACCGTC3′ Rev: 5′TAGCGTCCAGTGTGTACTGAC3′ IDT N/A GAPDH qPCR primers: Fwd: 5′TGCACCACCAACTGCTTAGC3′ Rev: 5′GGCATGGACTGTGGTCATGAG3′ IDT N/A Recombinant DNA mCherry This study N/A mCherry-TM6SF2 This study N/A (Continued on next page) 20 Cell Reports 45, 118116, October 27, 2026

    SYBR Green Assay:

    Article Title: TM6SF2 acts at ER membrane contact sites to enable RAB7-dependent endolysosomal VLDL secretion and maintain Golgi structure.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-APOB clone C1.4 Santa Cruz Biotechnology Cat# sc-13538; RRID:AB_626690 Mouse monoclonal anti-Rab7 (E9O7E) Cell Signaling Technology Cat# 95746; RRID:AB_2800252 Rabbit monoclonal anti-Rab7 (D95F2) Cell Signaling Technology Cat# 9367; RRID:AB_1904103 Mouse monoclonal anti-GFP clone B2 Santa Cruz Biotechnology Cat# sc-9996; RRID:AB_627695 Mouse monoclonal anti-FLAG-M2 Sigma-Aldrich Cat# F3165; RRID:AB_259529 Mouse monoclonal anti-HA-Tag (6E2) Cell Signaling Technology Cat# 2367; RRID:AB_10691311 Rabbit monoclonal anti-HA-Tag (C29F4) Cell Signaling Technology Cat# 3724; RRID:AB_1549585 Rabbit monoclonal anti-GST-Tag (91G1) Cell Signaling Technology Cat# 2625; RRID:AB_490796 Rabbit monoclonal anti-β-Actin (13E5) Cell Signaling Technology Cat# 4970; RRID:AB_2223172 Rabbit polyclonal anti-VAPA (VAP-33) Bethyl Laboratories Cat# A304-366A; RRID:AB_2620561 Rabbit polyclonal anti-VAPB Bethyl Laboratories Cat# A302-894A; RRID:AB_10663774 Rabbit monoclonal anti-STING (D2P2F) Cell Signaling Technology Cat# 13647; RRID:AB_2732796 Rabbit monoclonal anti-IRF-3 (D83B9) Cell Signaling Technology Cat# 4302; RRID:AB_1904036 Rabbit monoclonal anti-phospho-IRF-3 (Ser386) (E7J8G) Cell Signaling Technology Cat# 37829; RRID:AB_2799121 Rabbit monoclonal anti-TBK1/NAK (D1B4) Cell Signaling Technology Cat# 3504; RRID:AB_2255663 Rabbit monoclonal anti-phospho-TBK1/NAK (Ser172) (D52C2) Cell Signaling Technology Cat# 5483; RRID:AB_10693472 Rabbit monoclonal anti-NF-κB p65 (D14E12) Cell Signaling Technology Cat# 8242; RRID:AB_10859369 Rabbit monoclonal anti-phosphoNF-κB p65 (Ser536) (93H1) Cell Signaling Technology Cat# 3033; RRID:AB_331284 Rabbit monoclonal anti-GM130 (D6B1) Cell Signaling Technology Cat# 12480; RRID:AB_2797933 Sheep polyclonal anti-TGN46 Bio-Rad Laboratories Cat# AHP500GT; RRID:AB_2203291 Rabbit monoclonal anti-LAMP1 (D2D11) Cell Signaling Technology Cat# 9091; RRID:AB_2687579 Rabbit polyclonal anti-RAB5 Cell Signaling Technology Cat# 3547; RRID:AB_2300649 Rabbit monoclonal anti-EEA1 (C45B10) Cell Signaling Technology Cat# 3288; RRID:AB_2096811 Rabbit polyclonal anti-SEC31A Proteintech Cat# 17913-1-AP; RRID:AB_2186378 Rabbit monoclonal anti-LC3B (D11) Cell Signaling Technology Cat# 3868; RRID:AB_2137707 Goat poly polyclonal anti-human Albumin Bethyl Laboratories Cat# A80-229A; RRID:AB_67018 Mouse monoclonal anti-HNF4A Abcam Cat# ab41898; RRID:AB_732976 Rabbit polyclonal anti-PtdIns(4)P IgM Echelon Bioscience Cat# Z-P004; RRID:AB_11127796 Bacterial and virus strains NEB 5-alpha Competent E. coli (High Efficiency) New England BioLabs Cat# C2987H BL21 (DE3) E. coli New England BioLabs Cat# C2527H Biological samples Liver tissue samples from patients with MASH UCSF Department of Pathology N/A Chemicals, peptides, and recombinant proteins Recombinant human insulin GeminiBio Cat# 800–112P–100 Recombinant human FGFb Peprotech Cat# 100-18B Recombinant human BMP4 Peprotech Cat# 120-05ET Activin A StemCell Technologies Cat# 78001 Recombinant human HGF Peprotech Cat# 100–39 Recombinant human Oncostatin M Peprotech Cat# 300–10 CHIR99021 StemCell Technologies Cat# 72054 (Continued on next page) 18 Cell Reports 45, 118116, October 27, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Dexamethasone Sigma-Aldrich Cat# D4902 1-Thioglycerol Sigma-Aldrich Cat# M6145 PI103 MedChemExpress Cat# HY-10115 Sodium butyrate TCI Chemicals Cat# S0519 Y-27632 Tocris Cat# 1254 Non-essential amino acids (NEAA) Thermo Fisher Scientific Cat# 11140050 Biotin Sigma-Aldrich Cat# B4639 ADU-S100 MedChemExpress Cat# HY-12885 Oleic acid Sigma-Aldrich Cat# O1383 Polybrene Sigma-Aldrich Cat# H9268 DMEM medium Thermo Fisher Scientific Cat# 10569044 RPMI 1640 medium Thermo Fisher Scientific Cat# 11875093 mTeSR1 medium StemCell Technologies Cat# 85850 Hepatocyte Culture Medium (HCM) Lonza Cat# 185389 Opti-MEM medium Thermo Fisher Scientific Cat# 31985070 KnockOut DMEM medium Thermo Fisher Scientific Cat# 10829018 KnockOut Serum Replacement (KSR) Thermo Fisher Scientific Cat# 10828028 Gem21 without insulin GeminiBio Cat# 400–962 GlutaMAX Thermo Fisher Scientific Cat# 35050061 Cultrex Reduced Growth Factor Basement Membrane Extract (RGF-BME) R&D Systems Cat# 3433–001 Type I Collagen Corning Cat# 354249 IPTG Thermo Fisher Scientific Cat# 15529019 Halt protease inhibitor cocktail Thermo Fisher Scientific Cat# 78429 Halt phosphatase inhibitor cocktail Thermo Fisher Scientific Cat# 78420 Dynabeads protein G Thermo Fisher Scientific Cat# 10003D Glutathione Agarose Thermo Fisher Scientific Cat# 21516 LipidTOX Green Thermo Fisher Scientific Cat# H34475 Lipofectamine 3000 Thermo Fisher Scientific Cat# L3000001 Lipofectamine RNAiMAX Thermo Fisher Scientific Cat# 13778150 Critical commercial assays Triglycerides Liquid Stable Reagent Thermo Fisher Scientific Cat# TR22421 PureLink RNA Mini Kit Thermo Fisher Scientific Cat# 12183025 qScript cDNA SuperMix Quantabio Cat# 95048 SYBR Green PCR Master Mix Thermo Fisher Scientific Cat# 4309155 PureLink Genomic DNA Mini Kit Thermo Fisher Scientific Cat# K182001 HiFi DNA assembly kit New England BioLabs Cat# E2621 Pierce GST Protein Interaction Pull-Down Kit Thermo Fisher Scientific Cat# 21516 MycoAlert Mycoplasma Detection Kit Lonza Cat# LT07-318 Experimental models: Cell lines HEK293T ATCC Cat# CRL-3216 HepG2 ATCC Cat# HB-8065 Huh7 Japanese Collection of Research Bioresources Cell Bank Cat# JCRB0403 Healthy donor iPSC line CIRM iPSC repository at Fujifilm Cellular Dynamics N/A Same healthy donor iPSC line with TM6SF2 E167K gene edit Synthego N/A (Continued on next page) Cell Reports 45, 118116, October 27, 2026 19 .. REAGENT or RESOURCE SOURCE IDENTIFIER iPSC lines from patients with MASH CIRM iPSC repository at Fujifilm Cellular Dynamics N/A Primary human hepatocytes UCSF Liver Center N/A Oligonucleotides TM6SF2 E167K sgRNA: 5′GCAAAUACAGCUCCGAGAUC3′ Synthego N/A TM6SF2 E167K donor template: 5′ATGCCCTCTCTCCTGCACCATGGAAGGCAAATA CAGCTCCAAGATTAGGCCTGCCTTCTTCCTCACCA TCCCCTACCTGCT3′ Synthego N/A TM6SF2 KO sgRNA: 5′CTACGCGCTCAACCACGTCT3′ IDT N/A TM6SF2 DsiRNA 13.1: Sense: 5′AAGGAAGAGAUACCGGAAUUUUGGA3′ Antisense: 5′UCCAAAAUUCCGGUAUCUCUUCCUUCU3′ IDT N/A TM6SF2 DsiRNA 13.2: Sense: 5′CAGUAAAAACCCUUUCAAUUUCCAA3′ Antisense: 5′UUGGAAAUUGAAAGGGUUUUUACUGAA3′ IDT N/A TM6SF2 DsiRNA 13.3: Sense: 5′GACCUGGCCCUUGUCAUAUAUCUCA3′ Antisense: 5′UGAGAUAUAUGACAAGGGCCAGGUCAG3′ IDT N/A RAB7 DsiRNA 13.1: Sense: 5′AACCAGUAUGUGAAUAAGAAAUUCA3′ Antisense: 5′UGAAUUUCUUAUUCACAUACUGGUUCA3′ IDT N/A RAB7 DsiRNA 13.2: Sense: 5′GUGCUACAGCAAAAACAACAUUCCC3′ Antisense: 5′GGGAAUGUUGUUUUUGCUGUAGCACCA3′ IDT N/A RAB7 DsiRNA 13.3: Sense: 5′CCGUUAGAUCAGCAUUCUACUACAA3′ Antisense: 5′UUGUAGUAGAAUGCUGAUCUAACGGGA3′ IDT N/A TM6SF2 qPCR primers: Fwd: 5′TGGTGGTGCTTGATTGCCC3′ Rev: 5′CATGTGCGAGAACTGTGCCT3′ IDT N/A RAB7 qPCR primers: Fwd: 5′GTGTTGCTGAAGGTTATCATCCT3′ Rev: 5′GCTCCTATTGTGGCTTTGTACTG3′ IDT N/A CXCL10 qPCR primers: Fwd: 5′GTGGCATTCAAGGAGTACCTC3′ Rev: 5′TGATGGCCTTCGATTCTGGATT3′ IDT N/A ALB qPCR primers: Fwd: 5′AAGCTGCCTGCCTGTTGCCAA3′ Rev: 5′GGCGAGCTACTGCCCATGCTT3′ IDT N/A HNF4A qPCR primers: Fwd: 5′CCGCTTTGGAGAGCTGCT3′ Rev: 5′GGTTGTCAATCTTGGCCATGC3′ IDT N/A APOB qPCR primers: Fwd: 5′TGCTCCACTCACTTTACCGTC3′ Rev: 5′TAGCGTCCAGTGTGTACTGAC3′ IDT N/A GAPDH qPCR primers: Fwd: 5′TGCACCACCAACTGCTTAGC3′ Rev: 5′GGCATGGACTGTGGTCATGAG3′ IDT N/A Recombinant DNA mCherry This study N/A mCherry-TM6SF2 This study N/A (Continued on next page) 20 Cell Reports 45, 118116, October 27, 2026

    Polymerase Chain Reaction:

    Article Title: TM6SF2 acts at ER membrane contact sites to enable RAB7-dependent endolysosomal VLDL secretion and maintain Golgi structure.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-APOB clone C1.4 Santa Cruz Biotechnology Cat# sc-13538; RRID:AB_626690 Mouse monoclonal anti-Rab7 (E9O7E) Cell Signaling Technology Cat# 95746; RRID:AB_2800252 Rabbit monoclonal anti-Rab7 (D95F2) Cell Signaling Technology Cat# 9367; RRID:AB_1904103 Mouse monoclonal anti-GFP clone B2 Santa Cruz Biotechnology Cat# sc-9996; RRID:AB_627695 Mouse monoclonal anti-FLAG-M2 Sigma-Aldrich Cat# F3165; RRID:AB_259529 Mouse monoclonal anti-HA-Tag (6E2) Cell Signaling Technology Cat# 2367; RRID:AB_10691311 Rabbit monoclonal anti-HA-Tag (C29F4) Cell Signaling Technology Cat# 3724; RRID:AB_1549585 Rabbit monoclonal anti-GST-Tag (91G1) Cell Signaling Technology Cat# 2625; RRID:AB_490796 Rabbit monoclonal anti-β-Actin (13E5) Cell Signaling Technology Cat# 4970; RRID:AB_2223172 Rabbit polyclonal anti-VAPA (VAP-33) Bethyl Laboratories Cat# A304-366A; RRID:AB_2620561 Rabbit polyclonal anti-VAPB Bethyl Laboratories Cat# A302-894A; RRID:AB_10663774 Rabbit monoclonal anti-STING (D2P2F) Cell Signaling Technology Cat# 13647; RRID:AB_2732796 Rabbit monoclonal anti-IRF-3 (D83B9) Cell Signaling Technology Cat# 4302; RRID:AB_1904036 Rabbit monoclonal anti-phospho-IRF-3 (Ser386) (E7J8G) Cell Signaling Technology Cat# 37829; RRID:AB_2799121 Rabbit monoclonal anti-TBK1/NAK (D1B4) Cell Signaling Technology Cat# 3504; RRID:AB_2255663 Rabbit monoclonal anti-phospho-TBK1/NAK (Ser172) (D52C2) Cell Signaling Technology Cat# 5483; RRID:AB_10693472 Rabbit monoclonal anti-NF-κB p65 (D14E12) Cell Signaling Technology Cat# 8242; RRID:AB_10859369 Rabbit monoclonal anti-phosphoNF-κB p65 (Ser536) (93H1) Cell Signaling Technology Cat# 3033; RRID:AB_331284 Rabbit monoclonal anti-GM130 (D6B1) Cell Signaling Technology Cat# 12480; RRID:AB_2797933 Sheep polyclonal anti-TGN46 Bio-Rad Laboratories Cat# AHP500GT; RRID:AB_2203291 Rabbit monoclonal anti-LAMP1 (D2D11) Cell Signaling Technology Cat# 9091; RRID:AB_2687579 Rabbit polyclonal anti-RAB5 Cell Signaling Technology Cat# 3547; RRID:AB_2300649 Rabbit monoclonal anti-EEA1 (C45B10) Cell Signaling Technology Cat# 3288; RRID:AB_2096811 Rabbit polyclonal anti-SEC31A Proteintech Cat# 17913-1-AP; RRID:AB_2186378 Rabbit monoclonal anti-LC3B (D11) Cell Signaling Technology Cat# 3868; RRID:AB_2137707 Goat poly polyclonal anti-human Albumin Bethyl Laboratories Cat# A80-229A; RRID:AB_67018 Mouse monoclonal anti-HNF4A Abcam Cat# ab41898; RRID:AB_732976 Rabbit polyclonal anti-PtdIns(4)P IgM Echelon Bioscience Cat# Z-P004; RRID:AB_11127796 Bacterial and virus strains NEB 5-alpha Competent E. coli (High Efficiency) New England BioLabs Cat# C2987H BL21 (DE3) E. coli New England BioLabs Cat# C2527H Biological samples Liver tissue samples from patients with MASH UCSF Department of Pathology N/A Chemicals, peptides, and recombinant proteins Recombinant human insulin GeminiBio Cat# 800–112P–100 Recombinant human FGFb Peprotech Cat# 100-18B Recombinant human BMP4 Peprotech Cat# 120-05ET Activin A StemCell Technologies Cat# 78001 Recombinant human HGF Peprotech Cat# 100–39 Recombinant human Oncostatin M Peprotech Cat# 300–10 CHIR99021 StemCell Technologies Cat# 72054 (Continued on next page) 18 Cell Reports 45, 118116, October 27, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Dexamethasone Sigma-Aldrich Cat# D4902 1-Thioglycerol Sigma-Aldrich Cat# M6145 PI103 MedChemExpress Cat# HY-10115 Sodium butyrate TCI Chemicals Cat# S0519 Y-27632 Tocris Cat# 1254 Non-essential amino acids (NEAA) Thermo Fisher Scientific Cat# 11140050 Biotin Sigma-Aldrich Cat# B4639 ADU-S100 MedChemExpress Cat# HY-12885 Oleic acid Sigma-Aldrich Cat# O1383 Polybrene Sigma-Aldrich Cat# H9268 DMEM medium Thermo Fisher Scientific Cat# 10569044 RPMI 1640 medium Thermo Fisher Scientific Cat# 11875093 mTeSR1 medium StemCell Technologies Cat# 85850 Hepatocyte Culture Medium (HCM) Lonza Cat# 185389 Opti-MEM medium Thermo Fisher Scientific Cat# 31985070 KnockOut DMEM medium Thermo Fisher Scientific Cat# 10829018 KnockOut Serum Replacement (KSR) Thermo Fisher Scientific Cat# 10828028 Gem21 without insulin GeminiBio Cat# 400–962 GlutaMAX Thermo Fisher Scientific Cat# 35050061 Cultrex Reduced Growth Factor Basement Membrane Extract (RGF-BME) R&D Systems Cat# 3433–001 Type I Collagen Corning Cat# 354249 IPTG Thermo Fisher Scientific Cat# 15529019 Halt protease inhibitor cocktail Thermo Fisher Scientific Cat# 78429 Halt phosphatase inhibitor cocktail Thermo Fisher Scientific Cat# 78420 Dynabeads protein G Thermo Fisher Scientific Cat# 10003D Glutathione Agarose Thermo Fisher Scientific Cat# 21516 LipidTOX Green Thermo Fisher Scientific Cat# H34475 Lipofectamine 3000 Thermo Fisher Scientific Cat# L3000001 Lipofectamine RNAiMAX Thermo Fisher Scientific Cat# 13778150 Critical commercial assays Triglycerides Liquid Stable Reagent Thermo Fisher Scientific Cat# TR22421 PureLink RNA Mini Kit Thermo Fisher Scientific Cat# 12183025 qScript cDNA SuperMix Quantabio Cat# 95048 SYBR Green PCR Master Mix Thermo Fisher Scientific Cat# 4309155 PureLink Genomic DNA Mini Kit Thermo Fisher Scientific Cat# K182001 HiFi DNA assembly kit New England BioLabs Cat# E2621 Pierce GST Protein Interaction Pull-Down Kit Thermo Fisher Scientific Cat# 21516 MycoAlert Mycoplasma Detection Kit Lonza Cat# LT07-318 Experimental models: Cell lines HEK293T ATCC Cat# CRL-3216 HepG2 ATCC Cat# HB-8065 Huh7 Japanese Collection of Research Bioresources Cell Bank Cat# JCRB0403 Healthy donor iPSC line CIRM iPSC repository at Fujifilm Cellular Dynamics N/A Same healthy donor iPSC line with TM6SF2 E167K gene edit Synthego N/A (Continued on next page) Cell Reports 45, 118116, October 27, 2026 19 .. REAGENT or RESOURCE SOURCE IDENTIFIER iPSC lines from patients with MASH CIRM iPSC repository at Fujifilm Cellular Dynamics N/A Primary human hepatocytes UCSF Liver Center N/A Oligonucleotides TM6SF2 E167K sgRNA: 5′GCAAAUACAGCUCCGAGAUC3′ Synthego N/A TM6SF2 E167K donor template: 5′ATGCCCTCTCTCCTGCACCATGGAAGGCAAATA CAGCTCCAAGATTAGGCCTGCCTTCTTCCTCACCA TCCCCTACCTGCT3′ Synthego N/A TM6SF2 KO sgRNA: 5′CTACGCGCTCAACCACGTCT3′ IDT N/A TM6SF2 DsiRNA 13.1: Sense: 5′AAGGAAGAGAUACCGGAAUUUUGGA3′ Antisense: 5′UCCAAAAUUCCGGUAUCUCUUCCUUCU3′ IDT N/A TM6SF2 DsiRNA 13.2: Sense: 5′CAGUAAAAACCCUUUCAAUUUCCAA3′ Antisense: 5′UUGGAAAUUGAAAGGGUUUUUACUGAA3′ IDT N/A TM6SF2 DsiRNA 13.3: Sense: 5′GACCUGGCCCUUGUCAUAUAUCUCA3′ Antisense: 5′UGAGAUAUAUGACAAGGGCCAGGUCAG3′ IDT N/A RAB7 DsiRNA 13.1: Sense: 5′AACCAGUAUGUGAAUAAGAAAUUCA3′ Antisense: 5′UGAAUUUCUUAUUCACAUACUGGUUCA3′ IDT N/A RAB7 DsiRNA 13.2: Sense: 5′GUGCUACAGCAAAAACAACAUUCCC3′ Antisense: 5′GGGAAUGUUGUUUUUGCUGUAGCACCA3′ IDT N/A RAB7 DsiRNA 13.3: Sense: 5′CCGUUAGAUCAGCAUUCUACUACAA3′ Antisense: 5′UUGUAGUAGAAUGCUGAUCUAACGGGA3′ IDT N/A TM6SF2 qPCR primers: Fwd: 5′TGGTGGTGCTTGATTGCCC3′ Rev: 5′CATGTGCGAGAACTGTGCCT3′ IDT N/A RAB7 qPCR primers: Fwd: 5′GTGTTGCTGAAGGTTATCATCCT3′ Rev: 5′GCTCCTATTGTGGCTTTGTACTG3′ IDT N/A CXCL10 qPCR primers: Fwd: 5′GTGGCATTCAAGGAGTACCTC3′ Rev: 5′TGATGGCCTTCGATTCTGGATT3′ IDT N/A ALB qPCR primers: Fwd: 5′AAGCTGCCTGCCTGTTGCCAA3′ Rev: 5′GGCGAGCTACTGCCCATGCTT3′ IDT N/A HNF4A qPCR primers: Fwd: 5′CCGCTTTGGAGAGCTGCT3′ Rev: 5′GGTTGTCAATCTTGGCCATGC3′ IDT N/A APOB qPCR primers: Fwd: 5′TGCTCCACTCACTTTACCGTC3′ Rev: 5′TAGCGTCCAGTGTGTACTGAC3′ IDT N/A GAPDH qPCR primers: Fwd: 5′TGCACCACCAACTGCTTAGC3′ Rev: 5′GGCATGGACTGTGGTCATGAG3′ IDT N/A Recombinant DNA mCherry This study N/A mCherry-TM6SF2 This study N/A (Continued on next page) 20 Cell Reports 45, 118116, October 27, 2026

    Molecular Cloning:

    Article Title: TM6SF2 acts at ER membrane contact sites to enable RAB7-dependent endolysosomal VLDL secretion and maintain Golgi structure.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-APOB clone C1.4 Santa Cruz Biotechnology Cat# sc-13538; RRID:AB_626690 Mouse monoclonal anti-Rab7 (E9O7E) Cell Signaling Technology Cat# 95746; RRID:AB_2800252 Rabbit monoclonal anti-Rab7 (D95F2) Cell Signaling Technology Cat# 9367; RRID:AB_1904103 Mouse monoclonal anti-GFP clone B2 Santa Cruz Biotechnology Cat# sc-9996; RRID:AB_627695 Mouse monoclonal anti-FLAG-M2 Sigma-Aldrich Cat# F3165; RRID:AB_259529 Mouse monoclonal anti-HA-Tag (6E2) Cell Signaling Technology Cat# 2367; RRID:AB_10691311 Rabbit monoclonal anti-HA-Tag (C29F4) Cell Signaling Technology Cat# 3724; RRID:AB_1549585 Rabbit monoclonal anti-GST-Tag (91G1) Cell Signaling Technology Cat# 2625; RRID:AB_490796 Rabbit monoclonal anti-β-Actin (13E5) Cell Signaling Technology Cat# 4970; RRID:AB_2223172 Rabbit polyclonal anti-VAPA (VAP-33) Bethyl Laboratories Cat# A304-366A; RRID:AB_2620561 Rabbit polyclonal anti-VAPB Bethyl Laboratories Cat# A302-894A; RRID:AB_10663774 Rabbit monoclonal anti-STING (D2P2F) Cell Signaling Technology Cat# 13647; RRID:AB_2732796 Rabbit monoclonal anti-IRF-3 (D83B9) Cell Signaling Technology Cat# 4302; RRID:AB_1904036 Rabbit monoclonal anti-phospho-IRF-3 (Ser386) (E7J8G) Cell Signaling Technology Cat# 37829; RRID:AB_2799121 Rabbit monoclonal anti-TBK1/NAK (D1B4) Cell Signaling Technology Cat# 3504; RRID:AB_2255663 Rabbit monoclonal anti-phospho-TBK1/NAK (Ser172) (D52C2) Cell Signaling Technology Cat# 5483; RRID:AB_10693472 Rabbit monoclonal anti-NF-κB p65 (D14E12) Cell Signaling Technology Cat# 8242; RRID:AB_10859369 Rabbit monoclonal anti-phosphoNF-κB p65 (Ser536) (93H1) Cell Signaling Technology Cat# 3033; RRID:AB_331284 Rabbit monoclonal anti-GM130 (D6B1) Cell Signaling Technology Cat# 12480; RRID:AB_2797933 Sheep polyclonal anti-TGN46 Bio-Rad Laboratories Cat# AHP500GT; RRID:AB_2203291 Rabbit monoclonal anti-LAMP1 (D2D11) Cell Signaling Technology Cat# 9091; RRID:AB_2687579 Rabbit polyclonal anti-RAB5 Cell Signaling Technology Cat# 3547; RRID:AB_2300649 Rabbit monoclonal anti-EEA1 (C45B10) Cell Signaling Technology Cat# 3288; RRID:AB_2096811 Rabbit polyclonal anti-SEC31A Proteintech Cat# 17913-1-AP; RRID:AB_2186378 Rabbit monoclonal anti-LC3B (D11) Cell Signaling Technology Cat# 3868; RRID:AB_2137707 Goat poly polyclonal anti-human Albumin Bethyl Laboratories Cat# A80-229A; RRID:AB_67018 Mouse monoclonal anti-HNF4A Abcam Cat# ab41898; RRID:AB_732976 Rabbit polyclonal anti-PtdIns(4)P IgM Echelon Bioscience Cat# Z-P004; RRID:AB_11127796 Bacterial and virus strains NEB 5-alpha Competent E. coli (High Efficiency) New England BioLabs Cat# C2987H BL21 (DE3) E. coli New England BioLabs Cat# C2527H Biological samples Liver tissue samples from patients with MASH UCSF Department of Pathology N/A Chemicals, peptides, and recombinant proteins Recombinant human insulin GeminiBio Cat# 800–112P–100 Recombinant human FGFb Peprotech Cat# 100-18B Recombinant human BMP4 Peprotech Cat# 120-05ET Activin A StemCell Technologies Cat# 78001 Recombinant human HGF Peprotech Cat# 100–39 Recombinant human Oncostatin M Peprotech Cat# 300–10 CHIR99021 StemCell Technologies Cat# 72054 (Continued on next page) 18 Cell Reports 45, 118116, October 27, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Dexamethasone Sigma-Aldrich Cat# D4902 1-Thioglycerol Sigma-Aldrich Cat# M6145 PI103 MedChemExpress Cat# HY-10115 Sodium butyrate TCI Chemicals Cat# S0519 Y-27632 Tocris Cat# 1254 Non-essential amino acids (NEAA) Thermo Fisher Scientific Cat# 11140050 Biotin Sigma-Aldrich Cat# B4639 ADU-S100 MedChemExpress Cat# HY-12885 Oleic acid Sigma-Aldrich Cat# O1383 Polybrene Sigma-Aldrich Cat# H9268 DMEM medium Thermo Fisher Scientific Cat# 10569044 RPMI 1640 medium Thermo Fisher Scientific Cat# 11875093 mTeSR1 medium StemCell Technologies Cat# 85850 Hepatocyte Culture Medium (HCM) Lonza Cat# 185389 Opti-MEM medium Thermo Fisher Scientific Cat# 31985070 KnockOut DMEM medium Thermo Fisher Scientific Cat# 10829018 KnockOut Serum Replacement (KSR) Thermo Fisher Scientific Cat# 10828028 Gem21 without insulin GeminiBio Cat# 400–962 GlutaMAX Thermo Fisher Scientific Cat# 35050061 Cultrex Reduced Growth Factor Basement Membrane Extract (RGF-BME) R&D Systems Cat# 3433–001 Type I Collagen Corning Cat# 354249 IPTG Thermo Fisher Scientific Cat# 15529019 Halt protease inhibitor cocktail Thermo Fisher Scientific Cat# 78429 Halt phosphatase inhibitor cocktail Thermo Fisher Scientific Cat# 78420 Dynabeads protein G Thermo Fisher Scientific Cat# 10003D Glutathione Agarose Thermo Fisher Scientific Cat# 21516 LipidTOX Green Thermo Fisher Scientific Cat# H34475 Lipofectamine 3000 Thermo Fisher Scientific Cat# L3000001 Lipofectamine RNAiMAX Thermo Fisher Scientific Cat# 13778150 Critical commercial assays Triglycerides Liquid Stable Reagent Thermo Fisher Scientific Cat# TR22421 PureLink RNA Mini Kit Thermo Fisher Scientific Cat# 12183025 qScript cDNA SuperMix Quantabio Cat# 95048 SYBR Green PCR Master Mix Thermo Fisher Scientific Cat# 4309155 PureLink Genomic DNA Mini Kit Thermo Fisher Scientific Cat# K182001 HiFi DNA assembly kit New England BioLabs Cat# E2621 Pierce GST Protein Interaction Pull-Down Kit Thermo Fisher Scientific Cat# 21516 MycoAlert Mycoplasma Detection Kit Lonza Cat# LT07-318 Experimental models: Cell lines HEK293T ATCC Cat# CRL-3216 HepG2 ATCC Cat# HB-8065 Huh7 Japanese Collection of Research Bioresources Cell Bank Cat# JCRB0403 Healthy donor iPSC line CIRM iPSC repository at Fujifilm Cellular Dynamics N/A Same healthy donor iPSC line with TM6SF2 E167K gene edit Synthego N/A (Continued on next page) Cell Reports 45, 118116, October 27, 2026 19 .. REAGENT or RESOURCE SOURCE IDENTIFIER iPSC lines from patients with MASH CIRM iPSC repository at Fujifilm Cellular Dynamics N/A Primary human hepatocytes UCSF Liver Center N/A Oligonucleotides TM6SF2 E167K sgRNA: 5′GCAAAUACAGCUCCGAGAUC3′ Synthego N/A TM6SF2 E167K donor template: 5′ATGCCCTCTCTCCTGCACCATGGAAGGCAAATA CAGCTCCAAGATTAGGCCTGCCTTCTTCCTCACCA TCCCCTACCTGCT3′ Synthego N/A TM6SF2 KO sgRNA: 5′CTACGCGCTCAACCACGTCT3′ IDT N/A TM6SF2 DsiRNA 13.1: Sense: 5′AAGGAAGAGAUACCGGAAUUUUGGA3′ Antisense: 5′UCCAAAAUUCCGGUAUCUCUUCCUUCU3′ IDT N/A TM6SF2 DsiRNA 13.2: Sense: 5′CAGUAAAAACCCUUUCAAUUUCCAA3′ Antisense: 5′UUGGAAAUUGAAAGGGUUUUUACUGAA3′ IDT N/A TM6SF2 DsiRNA 13.3: Sense: 5′GACCUGGCCCUUGUCAUAUAUCUCA3′ Antisense: 5′UGAGAUAUAUGACAAGGGCCAGGUCAG3′ IDT N/A RAB7 DsiRNA 13.1: Sense: 5′AACCAGUAUGUGAAUAAGAAAUUCA3′ Antisense: 5′UGAAUUUCUUAUUCACAUACUGGUUCA3′ IDT N/A RAB7 DsiRNA 13.2: Sense: 5′GUGCUACAGCAAAAACAACAUUCCC3′ Antisense: 5′GGGAAUGUUGUUUUUGCUGUAGCACCA3′ IDT N/A RAB7 DsiRNA 13.3: Sense: 5′CCGUUAGAUCAGCAUUCUACUACAA3′ Antisense: 5′UUGUAGUAGAAUGCUGAUCUAACGGGA3′ IDT N/A TM6SF2 qPCR primers: Fwd: 5′TGGTGGTGCTTGATTGCCC3′ Rev: 5′CATGTGCGAGAACTGTGCCT3′ IDT N/A RAB7 qPCR primers: Fwd: 5′GTGTTGCTGAAGGTTATCATCCT3′ Rev: 5′GCTCCTATTGTGGCTTTGTACTG3′ IDT N/A CXCL10 qPCR primers: Fwd: 5′GTGGCATTCAAGGAGTACCTC3′ Rev: 5′TGATGGCCTTCGATTCTGGATT3′ IDT N/A ALB qPCR primers: Fwd: 5′AAGCTGCCTGCCTGTTGCCAA3′ Rev: 5′GGCGAGCTACTGCCCATGCTT3′ IDT N/A HNF4A qPCR primers: Fwd: 5′CCGCTTTGGAGAGCTGCT3′ Rev: 5′GGTTGTCAATCTTGGCCATGC3′ IDT N/A APOB qPCR primers: Fwd: 5′TGCTCCACTCACTTTACCGTC3′ Rev: 5′TAGCGTCCAGTGTGTACTGAC3′ IDT N/A GAPDH qPCR primers: Fwd: 5′TGCACCACCAACTGCTTAGC3′ Rev: 5′GGCATGGACTGTGGTCATGAG3′ IDT N/A Recombinant DNA mCherry This study N/A mCherry-TM6SF2 This study N/A (Continued on next page) 20 Cell Reports 45, 118116, October 27, 2026

    Pull Down Assay:

    Article Title: TM6SF2 acts at ER membrane contact sites to enable RAB7-dependent endolysosomal VLDL secretion and maintain Golgi structure.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-APOB clone C1.4 Santa Cruz Biotechnology Cat# sc-13538; RRID:AB_626690 Mouse monoclonal anti-Rab7 (E9O7E) Cell Signaling Technology Cat# 95746; RRID:AB_2800252 Rabbit monoclonal anti-Rab7 (D95F2) Cell Signaling Technology Cat# 9367; RRID:AB_1904103 Mouse monoclonal anti-GFP clone B2 Santa Cruz Biotechnology Cat# sc-9996; RRID:AB_627695 Mouse monoclonal anti-FLAG-M2 Sigma-Aldrich Cat# F3165; RRID:AB_259529 Mouse monoclonal anti-HA-Tag (6E2) Cell Signaling Technology Cat# 2367; RRID:AB_10691311 Rabbit monoclonal anti-HA-Tag (C29F4) Cell Signaling Technology Cat# 3724; RRID:AB_1549585 Rabbit monoclonal anti-GST-Tag (91G1) Cell Signaling Technology Cat# 2625; RRID:AB_490796 Rabbit monoclonal anti-β-Actin (13E5) Cell Signaling Technology Cat# 4970; RRID:AB_2223172 Rabbit polyclonal anti-VAPA (VAP-33) Bethyl Laboratories Cat# A304-366A; RRID:AB_2620561 Rabbit polyclonal anti-VAPB Bethyl Laboratories Cat# A302-894A; RRID:AB_10663774 Rabbit monoclonal anti-STING (D2P2F) Cell Signaling Technology Cat# 13647; RRID:AB_2732796 Rabbit monoclonal anti-IRF-3 (D83B9) Cell Signaling Technology Cat# 4302; RRID:AB_1904036 Rabbit monoclonal anti-phospho-IRF-3 (Ser386) (E7J8G) Cell Signaling Technology Cat# 37829; RRID:AB_2799121 Rabbit monoclonal anti-TBK1/NAK (D1B4) Cell Signaling Technology Cat# 3504; RRID:AB_2255663 Rabbit monoclonal anti-phospho-TBK1/NAK (Ser172) (D52C2) Cell Signaling Technology Cat# 5483; RRID:AB_10693472 Rabbit monoclonal anti-NF-κB p65 (D14E12) Cell Signaling Technology Cat# 8242; RRID:AB_10859369 Rabbit monoclonal anti-phosphoNF-κB p65 (Ser536) (93H1) Cell Signaling Technology Cat# 3033; RRID:AB_331284 Rabbit monoclonal anti-GM130 (D6B1) Cell Signaling Technology Cat# 12480; RRID:AB_2797933 Sheep polyclonal anti-TGN46 Bio-Rad Laboratories Cat# AHP500GT; RRID:AB_2203291 Rabbit monoclonal anti-LAMP1 (D2D11) Cell Signaling Technology Cat# 9091; RRID:AB_2687579 Rabbit polyclonal anti-RAB5 Cell Signaling Technology Cat# 3547; RRID:AB_2300649 Rabbit monoclonal anti-EEA1 (C45B10) Cell Signaling Technology Cat# 3288; RRID:AB_2096811 Rabbit polyclonal anti-SEC31A Proteintech Cat# 17913-1-AP; RRID:AB_2186378 Rabbit monoclonal anti-LC3B (D11) Cell Signaling Technology Cat# 3868; RRID:AB_2137707 Goat poly polyclonal anti-human Albumin Bethyl Laboratories Cat# A80-229A; RRID:AB_67018 Mouse monoclonal anti-HNF4A Abcam Cat# ab41898; RRID:AB_732976 Rabbit polyclonal anti-PtdIns(4)P IgM Echelon Bioscience Cat# Z-P004; RRID:AB_11127796 Bacterial and virus strains NEB 5-alpha Competent E. coli (High Efficiency) New England BioLabs Cat# C2987H BL21 (DE3) E. coli New England BioLabs Cat# C2527H Biological samples Liver tissue samples from patients with MASH UCSF Department of Pathology N/A Chemicals, peptides, and recombinant proteins Recombinant human insulin GeminiBio Cat# 800–112P–100 Recombinant human FGFb Peprotech Cat# 100-18B Recombinant human BMP4 Peprotech Cat# 120-05ET Activin A StemCell Technologies Cat# 78001 Recombinant human HGF Peprotech Cat# 100–39 Recombinant human Oncostatin M Peprotech Cat# 300–10 CHIR99021 StemCell Technologies Cat# 72054 (Continued on next page) 18 Cell Reports 45, 118116, October 27, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Dexamethasone Sigma-Aldrich Cat# D4902 1-Thioglycerol Sigma-Aldrich Cat# M6145 PI103 MedChemExpress Cat# HY-10115 Sodium butyrate TCI Chemicals Cat# S0519 Y-27632 Tocris Cat# 1254 Non-essential amino acids (NEAA) Thermo Fisher Scientific Cat# 11140050 Biotin Sigma-Aldrich Cat# B4639 ADU-S100 MedChemExpress Cat# HY-12885 Oleic acid Sigma-Aldrich Cat# O1383 Polybrene Sigma-Aldrich Cat# H9268 DMEM medium Thermo Fisher Scientific Cat# 10569044 RPMI 1640 medium Thermo Fisher Scientific Cat# 11875093 mTeSR1 medium StemCell Technologies Cat# 85850 Hepatocyte Culture Medium (HCM) Lonza Cat# 185389 Opti-MEM medium Thermo Fisher Scientific Cat# 31985070 KnockOut DMEM medium Thermo Fisher Scientific Cat# 10829018 KnockOut Serum Replacement (KSR) Thermo Fisher Scientific Cat# 10828028 Gem21 without insulin GeminiBio Cat# 400–962 GlutaMAX Thermo Fisher Scientific Cat# 35050061 Cultrex Reduced Growth Factor Basement Membrane Extract (RGF-BME) R&D Systems Cat# 3433–001 Type I Collagen Corning Cat# 354249 IPTG Thermo Fisher Scientific Cat# 15529019 Halt protease inhibitor cocktail Thermo Fisher Scientific Cat# 78429 Halt phosphatase inhibitor cocktail Thermo Fisher Scientific Cat# 78420 Dynabeads protein G Thermo Fisher Scientific Cat# 10003D Glutathione Agarose Thermo Fisher Scientific Cat# 21516 LipidTOX Green Thermo Fisher Scientific Cat# H34475 Lipofectamine 3000 Thermo Fisher Scientific Cat# L3000001 Lipofectamine RNAiMAX Thermo Fisher Scientific Cat# 13778150 Critical commercial assays Triglycerides Liquid Stable Reagent Thermo Fisher Scientific Cat# TR22421 PureLink RNA Mini Kit Thermo Fisher Scientific Cat# 12183025 qScript cDNA SuperMix Quantabio Cat# 95048 SYBR Green PCR Master Mix Thermo Fisher Scientific Cat# 4309155 PureLink Genomic DNA Mini Kit Thermo Fisher Scientific Cat# K182001 HiFi DNA assembly kit New England BioLabs Cat# E2621 Pierce GST Protein Interaction Pull-Down Kit Thermo Fisher Scientific Cat# 21516 MycoAlert Mycoplasma Detection Kit Lonza Cat# LT07-318 Experimental models: Cell lines HEK293T ATCC Cat# CRL-3216 HepG2 ATCC Cat# HB-8065 Huh7 Japanese Collection of Research Bioresources Cell Bank Cat# JCRB0403 Healthy donor iPSC line CIRM iPSC repository at Fujifilm Cellular Dynamics N/A Same healthy donor iPSC line with TM6SF2 E167K gene edit Synthego N/A (Continued on next page) Cell Reports 45, 118116, October 27, 2026 19 .. REAGENT or RESOURCE SOURCE IDENTIFIER iPSC lines from patients with MASH CIRM iPSC repository at Fujifilm Cellular Dynamics N/A Primary human hepatocytes UCSF Liver Center N/A Oligonucleotides TM6SF2 E167K sgRNA: 5′GCAAAUACAGCUCCGAGAUC3′ Synthego N/A TM6SF2 E167K donor template: 5′ATGCCCTCTCTCCTGCACCATGGAAGGCAAATA CAGCTCCAAGATTAGGCCTGCCTTCTTCCTCACCA TCCCCTACCTGCT3′ Synthego N/A TM6SF2 KO sgRNA: 5′CTACGCGCTCAACCACGTCT3′ IDT N/A TM6SF2 DsiRNA 13.1: Sense: 5′AAGGAAGAGAUACCGGAAUUUUGGA3′ Antisense: 5′UCCAAAAUUCCGGUAUCUCUUCCUUCU3′ IDT N/A TM6SF2 DsiRNA 13.2: Sense: 5′CAGUAAAAACCCUUUCAAUUUCCAA3′ Antisense: 5′UUGGAAAUUGAAAGGGUUUUUACUGAA3′ IDT N/A TM6SF2 DsiRNA 13.3: Sense: 5′GACCUGGCCCUUGUCAUAUAUCUCA3′ Antisense: 5′UGAGAUAUAUGACAAGGGCCAGGUCAG3′ IDT N/A RAB7 DsiRNA 13.1: Sense: 5′AACCAGUAUGUGAAUAAGAAAUUCA3′ Antisense: 5′UGAAUUUCUUAUUCACAUACUGGUUCA3′ IDT N/A RAB7 DsiRNA 13.2: Sense: 5′GUGCUACAGCAAAAACAACAUUCCC3′ Antisense: 5′GGGAAUGUUGUUUUUGCUGUAGCACCA3′ IDT N/A RAB7 DsiRNA 13.3: Sense: 5′CCGUUAGAUCAGCAUUCUACUACAA3′ Antisense: 5′UUGUAGUAGAAUGCUGAUCUAACGGGA3′ IDT N/A TM6SF2 qPCR primers: Fwd: 5′TGGTGGTGCTTGATTGCCC3′ Rev: 5′CATGTGCGAGAACTGTGCCT3′ IDT N/A RAB7 qPCR primers: Fwd: 5′GTGTTGCTGAAGGTTATCATCCT3′ Rev: 5′GCTCCTATTGTGGCTTTGTACTG3′ IDT N/A CXCL10 qPCR primers: Fwd: 5′GTGGCATTCAAGGAGTACCTC3′ Rev: 5′TGATGGCCTTCGATTCTGGATT3′ IDT N/A ALB qPCR primers: Fwd: 5′AAGCTGCCTGCCTGTTGCCAA3′ Rev: 5′GGCGAGCTACTGCCCATGCTT3′ IDT N/A HNF4A qPCR primers: Fwd: 5′CCGCTTTGGAGAGCTGCT3′ Rev: 5′GGTTGTCAATCTTGGCCATGC3′ IDT N/A APOB qPCR primers: Fwd: 5′TGCTCCACTCACTTTACCGTC3′ Rev: 5′TAGCGTCCAGTGTGTACTGAC3′ IDT N/A GAPDH qPCR primers: Fwd: 5′TGCACCACCAACTGCTTAGC3′ Rev: 5′GGCATGGACTGTGGTCATGAG3′ IDT N/A Recombinant DNA mCherry This study N/A mCherry-TM6SF2 This study N/A (Continued on next page) 20 Cell Reports 45, 118116, October 27, 2026



    Similar Products

    high capacity reverse transcriptase kit  (Bio-Rad)
    99
    Bio-Rad high capacity reverse transcriptase kit
    High Capacity Reverse Transcriptase Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/iscript+reverse+transcriptase+kit/iScript+cDNA+Synthesis+Kit/pmc13654763-216-9-17
    Average 99 stars, based on 1 article reviews
    high capacity reverse transcriptase kit - by Bioz Stars, 2026-11
    99/100 stars
    		  Buy from Supplier
    iscript reverse transcriptase 198  (Bio-Rad)
    99
    Bio-Rad iscript reverse transcriptase 198
    Iscript Reverse Transcriptase 198, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/iscript+reverse+transcriptase+kit/IScript+C+DNA+Synthesis+Kit/pm42095486-85-13-17
    Average 99 stars, based on 1 article reviews
    iscript reverse transcriptase 198 - by Bioz Stars, 2026-11
    99/100 stars
    		  Buy from Supplier
    iscript reverse transcriptase cdna synthesis kit  (Bio-Rad)
    99
    Bio-Rad iscript reverse transcriptase cdna synthesis kit
    Iscript Reverse Transcriptase Cdna Synthesis Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/iscript+reverse+transcriptase+kit/iScript+cDNA+Synthesis+Kit/pmc13080255-182-23-29
    Average 99 stars, based on 1 article reviews
    iscript reverse transcriptase cdna synthesis kit - by Bioz Stars, 2026-11
    99/100 stars
    		  Buy from Supplier
    iscript reverse transcriptase  (Bio-Rad)
    99
    Bio-Rad iscript reverse transcriptase
    Iscript Reverse Transcriptase, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/iscript+reverse+transcriptase+kit/IScript+C+DNA+Synthesis+Kit/pmc13055633-119-8-11
    Average 99 stars, based on 1 article reviews
    iscript reverse transcriptase - by Bioz Stars, 2026-11
    99/100 stars
    		  Buy from Supplier
    rna iscript reverse transcriptase  (Bio-Rad)
    99
    Bio-Rad rna iscript reverse transcriptase
    Rna Iscript Reverse Transcriptase, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/iscript+reverse+transcriptase+kit/IScript+C+DNA+Synthesis+Kit/pm41618616-126-7-11
    Average 99 stars, based on 1 article reviews
    rna iscript reverse transcriptase - by Bioz Stars, 2026-11
    99/100 stars
    		  Buy from Supplier
    reverse transcriptase enzyme  (Bio-Rad)
    99
    Bio-Rad reverse transcriptase enzyme
    Reverse Transcriptase Enzyme, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/iscript+reverse+transcriptase+kit/IScript+C+DNA+Synthesis+Kit/pmc13017563-150-10-14
    Average 99 stars, based on 1 article reviews
    reverse transcriptase enzyme - by Bioz Stars, 2026-11
    99/100 stars
    		  Buy from Supplier

    Image Search Results