transcription factor staining kit (Elabscience Biotechnology)
Elabscience Biotechnology is a verified supplier 
Elabscience Biotechnology manufactures this product 
94
Structured Review
Elabscience Biotechnology
transcription factor staining kit
Transcription Factor Staining Kit, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/iscript+reverse+transcriptase+kit/Foxp3%2FTranscription+Factor+Staining+Kit/pmc12859139-605-1-5
Average 94 stars, based on 21 article reviews
Transcription Factor Staining Kit, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/iscript+reverse+transcriptase+kit/Foxp3%2FTranscription+Factor+Staining+Kit/pmc12859139-605-1-5
Average 94 stars, based on 21 article reviews
transcription factor staining kit - by Bioz Stars,
2026-09
94/100 stars
Images
1) Product Images from "GPR4 promotes immune exclusion in colon cancer through LOXL2-mediated extracellular matrix remodeling"
Article Title: GPR4 promotes immune exclusion in colon cancer through LOXL2-mediated extracellular matrix remodeling
Journal: Nature Communications
doi: 10.1038/s41467-025-67967-z
Figure Legend Snippet: A GPR4 regulates JAK2 and STAT3 phosphorylation via western blotting. n = 3. Scale bar: 100 µm. B Representative IF images of STAT3-Y705 phosphorylation regulated by GPR4. C Quantitative analysis of STAT3-Y705 phosphorylation in GPR4 - OE SW480 cell line and ( D ) GPR4 - KD HCT116 cell lline. Data are presented as mean ± SD. Two-tailed t test, n = 3 biologically independent samples. E Static treatment reduces both pSTAT3-Y705 and the expression of LOXL2, COL1A1, and TGF-β in GPR4 - OE SW480 cells. F RT-PCR analysis for LOXL2 in SW480 cell lines after receiving Stattic treatment. Two-tailed t test, n = 3 biologically independent samples. G RT-PCR analysis for TGF-β in SW480 cell lines after receiving Stattic treatment. Data are presented as mean ± SD.Two-tailed t test, n = 3 biologically independent samples. H STAT3 transcription factor binding site. I In SW480 cells, STAT3 binds to the LOXL2 promoter region confirmed via ChIP assays. J STAT3 binds to the TGF-β promoter region confirmed via ChIP assays. K RT-PCR assesses the transcriptional activity of LOXL2 promoter regions and TGF-β promoter region L . Data are presented as mean ± SD. Two-tailed t test, n = 3 biologically independent samples. ns > 0.05.
Techniques Used: Phospho-proteomics, Western Blot, Two Tailed Test, Expressing, Reverse Transcription Polymerase Chain Reaction, Binding Assay, Activity Assay
Related Articles
Staining:Article Title: Electroacupuncture Ameliorates Cyclophosphamide-Induced Ovarian Impairment in Rats With Diminished Ovarian Reserve and is Associated With Th17/Treg-Related Immune Modulation. Article Snippet: See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense Intracellular Fixation/Permeabilization Buffer Kit (E-CK-A109, Elabscience, China). .. Treg/Th17 populations were detected using the Article Title: Mannosylated Fluoropolypeptide Nanovaccines Remodeling Tumor Immunosuppressive Microenvironment to Achieve Highly Potent Cancer Immunotherapy. Article Snippet: It is challenging for nanovaccines (NVs) to effectively deliver antigens/neoantigens to prime specifically potent immunities and remodel immunosuppressive tumor microenvironment (TME) for combating immune “cold” cancers.. Herein, a novel kind of mannosylated fluoropolypeptide NVs of MFPCOFG (i.e., mannosylated fluoropoly(D,L-cysteine) ovalbumin-loaded Fe2+-gallic acid) is designed that synergistically integrates triple antigen-metal-thermoimmunity to remodel immunosuppressive TME and achieve highly potent immunities.. MFPCOFG plus near-infrared irradiation (NIR) effectively facilitated antigen uptake and escape, induced the maturation and antigen cross-presentations of dendritic cells and macrophages, polarized anti-inflammatory macrophage phenotype M2 into tumoricial M1, primed potent CD4+/CD8+T cells responses, proinflammatory cytokines secretion and immune memory effects, showcasing triple antigen-metal-thermoimmunity outperforming combo/mono-immunity. Article Title: GPR4 promotes immune exclusion in colon cancer through LOXL2-mediated extracellular matrix remodeling. Article Snippet: Single-cell samples were stained for viability assess by using Zombie Yellow Fixable Viability Kit (Biolegend, 423103) at 4°C in the dark for 15 min. Then samples were stained by commercial antibodies for 20 min in dark, including anti-CD45-violent 450 (Elabscience, E-AB-F1136E), anti-CD3-APC (Elabscience, E-AB-F1013Q), anti-CD4-FITC (Elabscience, E-AB-F1097C), anti-CD8-PerCP (Elabscience, E-AB-F1104J). .. Subsequently, Article Title: GPR4 promotes immune exclusion in colon cancer through LOXL2-mediated extracellular matrix remodeling Article Snippet: Single-cell samples were stained for viability assess by using Zombie Yellow Fixable Viability Kit (Biolegend, 423103) at 4 °C in the dark for 15 min. Then samples were stained by commercial antibodies for 20 min in dark, including anti-CD45-violent 450 (Elabscience, E-AB-F1136E), anti-CD3-APC (Elabscience, E-AB-F1013Q), anti-CD4-FITC (Elabscience, E-AB-F1097C), anti-CD8-PerCP (Elabscience, E-AB-F1104J). .. Subsequently, Bioprocessing:Article Title: Electroacupuncture Ameliorates Cyclophosphamide-Induced Ovarian Impairment in Rats With Diminished Ovarian Reserve and is Associated With Th17/Treg-Related Immune Modulation. Article Snippet: See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense Intracellular Fixation/Permeabilization Buffer Kit (E-CK-A109, Elabscience, China). .. Treg/Th17 populations were detected using the |