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cdna  (New England Biolabs)


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    Structured Review

    New England Biolabs cdna
    Cdna, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 4556 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/iscript+reverse+transcriptase+kit/M-MuLV+Reverse+Transcriptase/pmc04233638-288-28-48
    Average 99 stars, based on 4556 article reviews
    cdna - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    cDNA Synthesis:

    Article Title: Effect of Bean-Derived Soluble Dietary Fibers on Macrophage Function In Vitro.
    Article Snippet: The PureLink RNA Mini Kit (12183018A) was obtained from Invitrogen (Waltham, MA, USA). .. The First Strand cDNA Synthesis (Quick Protocol) (M0253) components were purchased from New England BioLabs Inc. (Ipswich, MA, USA). .. The Brilliant II SYBR® Green QRT-PCR Master Mix with Low ROX was obtained from Agilent Technologies (Santa Clara, CA, USA).

    Article Title: Gonadotropin-Inhibitory Hormone (GnIH) Induces Glycolipid Metabolic Dysfunction in Porcine Ovarian Granulosa Cells via Wnt and AMPK Signaling Pathways.
    Article Snippet: Animal reproduction is closely linked to energy metabolism, and ovarian glycolipid metabolism disorders can lead to follicular abnormalities, reduced fertility, and infertility.. Granulosa cells (GCs), as key energy suppliers in the ovary, directly influence oocyte development, hormone secretion, and reproductive function.. However, the interaction between reproductive regulatory factors and ovarian energy metabolism is unclear.

    Article Title: Conserved pathogenesis of ancestral and contemporary Oropouche virus strains in a murine pregnancy model.
    Article Snippet: vRNA from OROV 240023 was extracted from 250 μl of viral supernatant using 750 μl of TRIzol LS Reagent (Ambion) and purified with the Direct-zol RNA MiniPrep kit (Zymo Research), following the manufacturer’s instructions. .. Complementary DNA (cDNA) synthesis was performed using M-MuLV reverse transcriptase (New England Biolabs). ..

    Synthesized:

    Article Title: The evolution of gene expression in allopolyploid cotton relative to parental diploids and induced haploids
    Article Snippet: Briefly, mRNA was purified from total RNA using poly-T oligo-attached magnetic beads, followed by fragmentation with divalent cations at elevated temperature in NEB Next First Strand Synthesis Reaction Buffer (5×) (NEB, USA). .. First-strand cDNA was synthesized using random hexamer primers and M-MuLV Reverse Transcriptase (RNase H), and second-strand cDNA was subsequently generated with DNA Polymerase I and RNase H. Overhangs were converted to blunt ends via exonuclease/polymerase activities, and after 3′ end adenylation, NEB Next Adaptors with hairpin loop structures (from a NEBNext Ultra RNA Library Prep Kit for Illumina, NEB, USA, Catalog #: E7530L) were ligated to the cDNA. ..

    Article Title: Overexpression of autophagy genes activates lipophagy in Arabidopsis.
    Article Snippet: AC CE PT ED M AN US CR IP T Cells possess an intrinsic recycling system called macroautophagy/autophagy, which delivers obsolete or damaged cytoplasmic components into the vacuole for degradation and reuse.. Autophagy-mediated breakdown of organellar membrane lipids supplies fatty acids for the synthesis of triacylglycerols (TAGs), which are then packaged into subcellular organelles called lipid droplets (LDs).. Conversely, autophagy contributes to TAG turnover by delivering LDs into vacuoles for breakdown by resident acid lipases.

    Article Title: Targeting Mettl8-Tcf1 axis promotes CD8 + T PEX differentiation and antitumor immunity
    Article Snippet: Fragmentation was carried out using divalent cations under elevated temperature in NEBNext First Strand Synthesis Reaction Buffer (5×). .. First, strand cDNA was synthesized using random hexamer primer and M-MuLV Reverse Transcriptase (NEB). ..

    Random Hexamer:

    Article Title: The evolution of gene expression in allopolyploid cotton relative to parental diploids and induced haploids
    Article Snippet: Briefly, mRNA was purified from total RNA using poly-T oligo-attached magnetic beads, followed by fragmentation with divalent cations at elevated temperature in NEB Next First Strand Synthesis Reaction Buffer (5×) (NEB, USA). .. First-strand cDNA was synthesized using random hexamer primers and M-MuLV Reverse Transcriptase (RNase H), and second-strand cDNA was subsequently generated with DNA Polymerase I and RNase H. Overhangs were converted to blunt ends via exonuclease/polymerase activities, and after 3′ end adenylation, NEB Next Adaptors with hairpin loop structures (from a NEBNext Ultra RNA Library Prep Kit for Illumina, NEB, USA, Catalog #: E7530L) were ligated to the cDNA. ..

    Article Title: Targeting Mettl8-Tcf1 axis promotes CD8 + T PEX differentiation and antitumor immunity
    Article Snippet: Fragmentation was carried out using divalent cations under elevated temperature in NEBNext First Strand Synthesis Reaction Buffer (5×). .. First, strand cDNA was synthesized using random hexamer primer and M-MuLV Reverse Transcriptase (NEB). ..

    Article Title: Curcumin suppresses HNSCC tumorigenesis through directly targeting FOSL1/JUN.
    Article Snippet: Natural products and affordable herbal medications have emerged as promising alternatives for cancer therapy, particularly for patients with limited access to standard treatments.. Among these, curcumin, the bioactive polyphenol from Curcuma longa (turmeric), has shown broad anti-cancer potential.. However, its molecular mechanism remains poorly defined, especially for head and neck squamous cell carcinoma (HNSCC).

    Reverse Transcription:

    Article Title: The evolution of gene expression in allopolyploid cotton relative to parental diploids and induced haploids
    Article Snippet: Briefly, mRNA was purified from total RNA using poly-T oligo-attached magnetic beads, followed by fragmentation with divalent cations at elevated temperature in NEB Next First Strand Synthesis Reaction Buffer (5×) (NEB, USA). .. First-strand cDNA was synthesized using random hexamer primers and M-MuLV Reverse Transcriptase (RNase H), and second-strand cDNA was subsequently generated with DNA Polymerase I and RNase H. Overhangs were converted to blunt ends via exonuclease/polymerase activities, and after 3′ end adenylation, NEB Next Adaptors with hairpin loop structures (from a NEBNext Ultra RNA Library Prep Kit for Illumina, NEB, USA, Catalog #: E7530L) were ligated to the cDNA. ..

    Article Title: Molecular characterization of Cdh12-SCON conditional knockout mice reveals unexpected splicing changes
    Article Snippet: Total RNA was isolated and purified from the forebrain using the RNeasy Plus Mini Kit (Qiagen; 74134). .. RNA concentration was measured with NanoDrop and 500 ng of total RNA was reverse transcribed into cDNA using NEB’s standard first strand synthesis protocol with M-MulV reverse transcriptase (NEB; M0253), 60 μM random primer mix (NEB; S1330), 10 mM dNTP, and RNase inhibitor (NEB; M0314). ..

    Article Title: Overexpression of autophagy genes activates lipophagy in Arabidopsis.
    Article Snippet: AC CE PT ED M AN US CR IP T Cells possess an intrinsic recycling system called macroautophagy/autophagy, which delivers obsolete or damaged cytoplasmic components into the vacuole for degradation and reuse.. Autophagy-mediated breakdown of organellar membrane lipids supplies fatty acids for the synthesis of triacylglycerols (TAGs), which are then packaged into subcellular organelles called lipid droplets (LDs).. Conversely, autophagy contributes to TAG turnover by delivering LDs into vacuoles for breakdown by resident acid lipases.

    Article Title: Targeting Mettl8-Tcf1 axis promotes CD8 + T PEX differentiation and antitumor immunity
    Article Snippet: Fragmentation was carried out using divalent cations under elevated temperature in NEBNext First Strand Synthesis Reaction Buffer (5×). .. First, strand cDNA was synthesized using random hexamer primer and M-MuLV Reverse Transcriptase (NEB). ..

    Article Title: Gonadotropin-Inhibitory Hormone (GnIH) Induces Glycolipid Metabolic Dysfunction in Porcine Ovarian Granulosa Cells via Wnt and AMPK Signaling Pathways.
    Article Snippet: Animal reproduction is closely linked to energy metabolism, and ovarian glycolipid metabolism disorders can lead to follicular abnormalities, reduced fertility, and infertility.. Granulosa cells (GCs), as key energy suppliers in the ovary, directly influence oocyte development, hormone secretion, and reproductive function.. However, the interaction between reproductive regulatory factors and ovarian energy metabolism is unclear.

    Article Title: Curcumin suppresses HNSCC tumorigenesis through directly targeting FOSL1/JUN.
    Article Snippet: Natural products and affordable herbal medications have emerged as promising alternatives for cancer therapy, particularly for patients with limited access to standard treatments.. Among these, curcumin, the bioactive polyphenol from Curcuma longa (turmeric), has shown broad anti-cancer potential.. However, its molecular mechanism remains poorly defined, especially for head and neck squamous cell carcinoma (HNSCC).

    Article Title: Conserved pathogenesis of ancestral and contemporary Oropouche virus strains in a murine pregnancy model.
    Article Snippet: vRNA from OROV 240023 was extracted from 250 μl of viral supernatant using 750 μl of TRIzol LS Reagent (Ambion) and purified with the Direct-zol RNA MiniPrep kit (Zymo Research), following the manufacturer’s instructions. .. Complementary DNA (cDNA) synthesis was performed using M-MuLV reverse transcriptase (New England Biolabs). ..

    Generated:

    Article Title: The evolution of gene expression in allopolyploid cotton relative to parental diploids and induced haploids
    Article Snippet: Briefly, mRNA was purified from total RNA using poly-T oligo-attached magnetic beads, followed by fragmentation with divalent cations at elevated temperature in NEB Next First Strand Synthesis Reaction Buffer (5×) (NEB, USA). .. First-strand cDNA was synthesized using random hexamer primers and M-MuLV Reverse Transcriptase (RNase H), and second-strand cDNA was subsequently generated with DNA Polymerase I and RNase H. Overhangs were converted to blunt ends via exonuclease/polymerase activities, and after 3′ end adenylation, NEB Next Adaptors with hairpin loop structures (from a NEBNext Ultra RNA Library Prep Kit for Illumina, NEB, USA, Catalog #: E7530L) were ligated to the cDNA. ..

    Concentration Assay:

    Article Title: Molecular characterization of Cdh12-SCON conditional knockout mice reveals unexpected splicing changes
    Article Snippet: Total RNA was isolated and purified from the forebrain using the RNeasy Plus Mini Kit (Qiagen; 74134). .. RNA concentration was measured with NanoDrop and 500 ng of total RNA was reverse transcribed into cDNA using NEB’s standard first strand synthesis protocol with M-MulV reverse transcriptase (NEB; M0253), 60 μM random primer mix (NEB; S1330), 10 mM dNTP, and RNase inhibitor (NEB; M0314). ..

    Real-time Polymerase Chain Reaction:

    Article Title: Curcumin suppresses HNSCC tumorigenesis through directly targeting FOSL1/JUN.
    Article Snippet: Natural products and affordable herbal medications have emerged as promising alternatives for cancer therapy, particularly for patients with limited access to standard treatments.. Among these, curcumin, the bioactive polyphenol from Curcuma longa (turmeric), has shown broad anti-cancer potential.. However, its molecular mechanism remains poorly defined, especially for head and neck squamous cell carcinoma (HNSCC).



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