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Structured Review

Promega superscript reverse transcriptase kit
Superscript Reverse Transcriptase Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/iscript+reverse+transcriptase+kit/superscript+first+strand+synthesis+kit/bio_rxiv__766063-49-14-18
Average 90 stars, based on 1 article reviews
superscript reverse transcriptase kit - by Bioz Stars, 2026-11
90/100 stars

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Related Articles

other:

Article Title: Angiogenesis-related lncRNAs predict the prognosis signature of stomach adenocarcinoma
Article Snippet: RNA (2 μg) was reversely transcribed into cDNA using a Superscript First-strand Synthesis system (Promega, USA) according to the manufacturer’s instructions.

Article Title: Investigation of the potential of leukoreduction filters in the creation of anti-inflammatory compound.
Article Snippet: To perform quantitative real-time polymerase chain reaction (RT-PCR), oligo dT primers and the SuperScript reverse S. Balagholi et al. Transfusion and Apheresis Science 62 (2023) 103520 transcriptase kit (Promega, USA) were used for reverse transcription.

Article Title: Hollow hydroxyapatite microsphere: a promising carrier for bone tissue engineering.
Article Snippet: Hydroxyapatite (HA) microsphere with abundant ordered mesoporous and large hollow structure was prepared at hydrothermal condition with the aid of citrate.. The obtained microsphere was performed as a drug delivery carrier to evaluate its drug loading/releasing behaviour using alendronate (AL) as a model drug.. Results revealed that HA particles performed high AL-loading capability (46.8 wt % with respect to the powdered starting materials) and excellent AL-releasing behaviour (sustained drug releasing profile without burst release).

Article Title: miR-9-5p promotes myogenic differentiation via the Dlx3/Myf5 axis
Article Snippet: RNA (2.5 μg) was reverse-transcribed into cDNA using the Superscript first-strand synthesis system (Promega, Madison, WI, USA), and 1 μg RNA was reverse-transcribed using the miDETECT A TrackTM miRNA qPCR kit (RiboBio, Guangzhou, China) following the manufacturers’ instructions. qPCR was carried out as previously described ( ).

Luciferase:

Article Title: Isorhapontigenin inhibition of basal muscle-invasive bladder cancer attributed to its downregulation of SNHG1 and DNMT3b
Article Snippet: .. The reagents used, including the dual luciferase assay kit and TRIzol, along with the SuperScript First Strand Synthesis system, were procured from Promega (Madison, WI, USA) and Invitrogen (Grand Island, NY, USA), respectively. .. The substrate for the luciferase assay was also obtained from Promega (Madison, WI, USA).

Synthesized:

Article Title: A novel antimicrobial peptide Larimicin 78-102 from large yellow croaker (Larimichthys crocea) shows potent antibacterial activity in vitro and enhances resistance to vibrio fluvialis infection in vivo.
Article Snippet: Antimicrobial peptides (AMPs) are considered a key component of innate immunity, playing a vital role in host defense.. In the study, a novel functional gene, named Larimicin, was identified from large yellow croaker Larimichthys crocea.. The Larimicin gene was widely distributed in multiple tissues of healthy L. crocea and was significantly induced in the liver after Vibrio alginolyticus or Vibrio parahaemolyticus infection.

Reverse Transcription:

Article Title: A novel antimicrobial peptide Larimicin 78-102 from large yellow croaker (Larimichthys crocea) shows potent antibacterial activity in vitro and enhances resistance to vibrio fluvialis infection in vivo.
Article Snippet: Antimicrobial peptides (AMPs) are considered a key component of innate immunity, playing a vital role in host defense.. In the study, a novel functional gene, named Larimicin, was identified from large yellow croaker Larimichthys crocea.. The Larimicin gene was widely distributed in multiple tissues of healthy L. crocea and was significantly induced in the liver after Vibrio alginolyticus or Vibrio parahaemolyticus infection.

Article Title: Comprehensive Analysis Revealed the Potential Roles of N 6 -Methyladenosine (m 6 A) Mediating E. coli F18 Susceptibility in IPEC-J2 Cells
Article Snippet: Trizol (Invitrogen, Shanghai, China) was used to extract total RNAs from duodenal tissues or IPEC-J2 cells. .. Next, the reverse transcription of RNA was conducted using a Superscript First-Strand Synthesis system (Promega, Madison, WI, USA). .. SYBR Green PCR Master Mix (Applied Biosystems, Foster City, CA, USA) was employed to conduct qPCR analysis.

Article Title: Investigation of the potential of leukoreduction filters in the creation of anti-inflammatory compound
Article Snippet: The concentration and purity of the isolated RNA were determined using a NanoDrop spectrophotometer (Thermo Fisher Scientific; Wilmington, DE, USA), and the integrity of RNA was verified by agarose gel electrophoresis. .. To perform quantitative real-time polymerase chain reaction (RT-PCR), oligo dT primers and the SuperScript reverse transcriptase kit (Promega, USA) were used for reverse transcription. .. An EvaGreen QPCR master mix (Solis BioDyne, Estonia) was used for RT-PCR, with the following PCR parameters: initial denaturation (one cycle at 95_C for 15 min); denaturation, amplification, and quantification for 40 cycles at 95_C for 15 s, 56–64_C for 17 s, and 72_C for 25 s; melting curve at 65_C, with the temperature being gradually increased to 95_C.

Real-time Polymerase Chain Reaction:

Article Title: Investigation of the potential of leukoreduction filters in the creation of anti-inflammatory compound
Article Snippet: The concentration and purity of the isolated RNA were determined using a NanoDrop spectrophotometer (Thermo Fisher Scientific; Wilmington, DE, USA), and the integrity of RNA was verified by agarose gel electrophoresis. .. To perform quantitative real-time polymerase chain reaction (RT-PCR), oligo dT primers and the SuperScript reverse transcriptase kit (Promega, USA) were used for reverse transcription. .. An EvaGreen QPCR master mix (Solis BioDyne, Estonia) was used for RT-PCR, with the following PCR parameters: initial denaturation (one cycle at 95_C for 15 min); denaturation, amplification, and quantification for 40 cycles at 95_C for 15 s, 56–64_C for 17 s, and 72_C for 25 s; melting curve at 65_C, with the temperature being gradually increased to 95_C.



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