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iscript advanced cdna synthesis kit  (Bio-Rad)


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    Structured Review

    Bio-Rad iscript advanced cdna synthesis kit

    Iscript Advanced Cdna Synthesis Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 590 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/iscript+advanced+reverse+transcriptase+kit/ISCRIPT+GDNA+CLR+CDNA+KT%2C100RX/pmc06264919-60-0-6
    Average 99 stars, based on 590 article reviews
    iscript advanced cdna synthesis kit - by Bioz Stars, 2026-11
    99/100 stars

    Images

    1) Product Images from "Complementary Wnt Sources Regulate Lymphatic Vascular Development via PROX1-Dependent Wnt/β-Catenin Signaling"

    Article Title: Complementary Wnt Sources Regulate Lymphatic Vascular Development via PROX1-Dependent Wnt/β-Catenin Signaling

    Journal: Cell reports

    doi: 10.1016/j.celrep.2018.09.049


    Figure Legend Snippet:

    Techniques Used: Virus, Recombinant, Electron Microscopy, Transfection, SYBR Green Assay, cDNA Synthesis, Bicinchoninic Acid Protein Assay, Software

    Related Articles

    Reverse Transcription:

    Article Title: Exacerbated ATP transmission in the carotid body is linked to glomus cell expansion in spontaneously hypertensive rats
    Article Snippet: figure legend Exacerbated ATP signalling in the carotid body (CB) of spontaneously hypertensive rats (SHRs) arises from expansion of the chemosensory glomus cell compartment and reduced extracellular ATP metabolism, © 2026 The Author(s).. The Journal of Physiology published by John Wiley & Sons Ltd on behalf of The Physiological Society.. DOI: 10.1113/JP288931 This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial

    Article Title: Autophagy dependent HIF1α proteostasis is compromised in models of PEX1 deficiencies
    Article Snippet: Genomic DNA in the lysate was sheared using the Qiagen QIAshredder spin columns (Qiagen #79656) prior to RNA extraction. .. 1 μg of RNA was reverse transcribed with the iScript cDNA synthesis system (BioRad #1725035) according to the manufacturer’s protocol. .. The cDNA was diluted 10-fold in nuclease-free water and 0.2% was used as input for SYBR green based gene-specific quantitative PCR (BioRad #1725271).

    Article Title: Diaphragm-specific effects of L-citrulline in mdx mice highlight its potential as adjuvant of standard therapy in Duchenne muscular dystrophy.
    Article Snippet: 2.2.4 | Isolation of total RNA, reverse transcription and qRT-PCR Total RNA was isolated from DIA and gastrocnemius (GC) muscles after homogenization, with Trizol (10296028, Life Technologies, CA, USA) and quantified for yield and purity by spectrophotometry (ND1000 NanoDrop, Thermo Fisher Scientific). .. Variable amounts of RNA (between 1 and 2 μg) were then reverse transcribed to cDNA with iScript gDNA Clear cDNA Synthesis Kit (172-5035 Bio-Rad Laboratories, Inc., CA, USA) following manufacturer instructions. qRT-PCR was performed via the CFX384 Connect Real-Time PCR System (Bio-Rad, Hercules, CA, USA) by using PrimePCR assays SYBR® green with custom-made plates. ..

    cDNA Synthesis:

    Article Title: Exacerbated ATP transmission in the carotid body is linked to glomus cell expansion in spontaneously hypertensive rats
    Article Snippet: figure legend Exacerbated ATP signalling in the carotid body (CB) of spontaneously hypertensive rats (SHRs) arises from expansion of the chemosensory glomus cell compartment and reduced extracellular ATP metabolism, © 2026 The Author(s).. The Journal of Physiology published by John Wiley & Sons Ltd on behalf of The Physiological Society.. DOI: 10.1113/JP288931 This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial

    Article Title: Interleukin-17A directly triggers Epstein-Barr virus lytic reactivation in latency I human B-cell models.
    Article Snippet: Whole cell RNA preparations were carried out using TRIzol reagent (Thermo Fisher, Cat# 15596) according to the vendor’s recommended protocol. .. cDNA was synthesized from total RNA using iScript gDNA Clear cDNA Synthesis Kit (Bio-Rad, Cat# 172-5035). .. qPCR analysis was performed using iQ SYBR Green Supermix (Bio-Rad, Cat# 170-8882) on a Bio-Rad CFX96 instrument as follows: 1 μL of cDNA and 1 μL of 10 μM primers were mixed with 10 μL of SYBR Green Supermix and 8 μL nucleasefree H2O to a 20 μL reaction volume.

    Article Title: Autophagy dependent HIF1α proteostasis is compromised in models of PEX1 deficiencies
    Article Snippet: Genomic DNA in the lysate was sheared using the Qiagen QIAshredder spin columns (Qiagen #79656) prior to RNA extraction. .. 1 μg of RNA was reverse transcribed with the iScript cDNA synthesis system (BioRad #1725035) according to the manufacturer’s protocol. .. The cDNA was diluted 10-fold in nuclease-free water and 0.2% was used as input for SYBR green based gene-specific quantitative PCR (BioRad #1725271).

    Article Title: Human iPSC-Derived Dorsal Root Ganglion Organoid Modeling of Chemotherapy-Induced Peripheral Neuropathy
    Article Snippet: .. RNA was extracted from iDRGOs using TRIzol (ThermoFisher Scientific TM , Catalog No., Waltham, MA, USA), chloroform (Sigma-Aldrich, Catalog No., St. Louis, MO, USA), and isopropanol precipitation. cDNA was synthesized from RNA using iScript gDNA cDNA Synthesis Kit (BioRAD, Catalog No. 172503, Hercules, CA, USA). .. Quantitative RT-PCR was performed using iTaq Universal SYBR Green One-Step Kit (BioRAD, Catalog No. 1725150, Hercules, CA, USA).

    Article Title: Diaphragm-specific effects of L-citrulline in mdx mice highlight its potential as adjuvant of standard therapy in Duchenne muscular dystrophy.
    Article Snippet: 2.2.4 | Isolation of total RNA, reverse transcription and qRT-PCR Total RNA was isolated from DIA and gastrocnemius (GC) muscles after homogenization, with Trizol (10296028, Life Technologies, CA, USA) and quantified for yield and purity by spectrophotometry (ND1000 NanoDrop, Thermo Fisher Scientific). .. Variable amounts of RNA (between 1 and 2 μg) were then reverse transcribed to cDNA with iScript gDNA Clear cDNA Synthesis Kit (172-5035 Bio-Rad Laboratories, Inc., CA, USA) following manufacturer instructions. qRT-PCR was performed via the CFX384 Connect Real-Time PCR System (Bio-Rad, Hercules, CA, USA) by using PrimePCR assays SYBR® green with custom-made plates. ..

    Article Title: Stage-specific CHI3L1/YKL-40 signaling controls generation of oligodendrocyte precursor cells through IL13Rα2-mediated ferroptosis
    Article Snippet: Acetylcysteine (N-Acetylcysteine, MCE: HY-B0215), Azeliragon (TTP488, MCE: HY-50682), Deferoxamine Mesylate (MCE: HY-B0988), Aspartic acid (MCE: HY-42068), FPS-ZM1 (MCE: HY-19370), 1,5-Isoquinolinediol (MCE: HY-W015422), Z-VAD-FMK (MCE: HY-16658B), Necrostatin-1 (MCE: HY-15760), Ferrostatin-1 (MCE: HY-100579), Disulfiram (Tetraethylthiuram disulfide, MCE: HY-B0240) were incubated in culture as indicated. .. mRNA was harvested with the Qiagen RNeasy Mini Kit (Qiagen #74104). cDNA was synthesized following the iScriptTM gDNA Clear cDNA Synthesis Kit (Bio-Rad #1725035). .. SYBR green probes (obtained from Bio-Rad) and FAM probes (obtained from iDT) targeting genes of interest were applied to evaluate gene expression under the CFX96 Touch Real-Time PCR Detection System.

    Article Title: Long-read RNA sequencing identifies non-coding isoform switching as a regulator of cell fate
    Article Snippet: .. cDNA was synthesized from 500ng of RNA by oligo-dT and random priming with iScriptTM gDNA Clear cDNA Synthesis Kit (Bio-Rad #1725035), according to the manufacturer’s instructions. .. cDNA was diluted ten times and qPCRs were performed using SYBRTM Select Master Mix (ThermoFisher Scientific #4472908) on a CFX384Touch real-timePCRdetection system (Bio-Rad).

    Article Title: Human iPSC-Derived Dorsal Root Ganglion Organoid Modeling of Chemotherapy-Induced Peripheral Neuropathy.
    Article Snippet: .. RNA was extracted from iDRGOs using TRIzol (ThermoFisher ScientificTM, Catalog No., Waltham, MA, USA), chloroform (Sigma-Aldrich, Catalog No., St. Louis, MO, USA), and isopropanol precipitation. cDNA was synthesized from RNA using iScript gDNA cDNA Synthesis Kit (BioRAD, Catalog No. 172503, Hercules, CA, USA). .. Quantitative RT-PCR was performed using iTaq Universal SYBR Green One-Step Kit (BioRAD, Catalog No. 1725150, Hercules, CA, USA).

    Synthesized:

    Article Title: Interleukin-17A directly triggers Epstein-Barr virus lytic reactivation in latency I human B-cell models.
    Article Snippet: Whole cell RNA preparations were carried out using TRIzol reagent (Thermo Fisher, Cat# 15596) according to the vendor’s recommended protocol. .. cDNA was synthesized from total RNA using iScript gDNA Clear cDNA Synthesis Kit (Bio-Rad, Cat# 172-5035). .. qPCR analysis was performed using iQ SYBR Green Supermix (Bio-Rad, Cat# 170-8882) on a Bio-Rad CFX96 instrument as follows: 1 μL of cDNA and 1 μL of 10 μM primers were mixed with 10 μL of SYBR Green Supermix and 8 μL nucleasefree H2O to a 20 μL reaction volume.

    Article Title: Human iPSC-Derived Dorsal Root Ganglion Organoid Modeling of Chemotherapy-Induced Peripheral Neuropathy
    Article Snippet: .. RNA was extracted from iDRGOs using TRIzol (ThermoFisher Scientific TM , Catalog No., Waltham, MA, USA), chloroform (Sigma-Aldrich, Catalog No., St. Louis, MO, USA), and isopropanol precipitation. cDNA was synthesized from RNA using iScript gDNA cDNA Synthesis Kit (BioRAD, Catalog No. 172503, Hercules, CA, USA). .. Quantitative RT-PCR was performed using iTaq Universal SYBR Green One-Step Kit (BioRAD, Catalog No. 1725150, Hercules, CA, USA).

    Article Title: Stage-specific CHI3L1/YKL-40 signaling controls generation of oligodendrocyte precursor cells through IL13Rα2-mediated ferroptosis
    Article Snippet: Acetylcysteine (N-Acetylcysteine, MCE: HY-B0215), Azeliragon (TTP488, MCE: HY-50682), Deferoxamine Mesylate (MCE: HY-B0988), Aspartic acid (MCE: HY-42068), FPS-ZM1 (MCE: HY-19370), 1,5-Isoquinolinediol (MCE: HY-W015422), Z-VAD-FMK (MCE: HY-16658B), Necrostatin-1 (MCE: HY-15760), Ferrostatin-1 (MCE: HY-100579), Disulfiram (Tetraethylthiuram disulfide, MCE: HY-B0240) were incubated in culture as indicated. .. mRNA was harvested with the Qiagen RNeasy Mini Kit (Qiagen #74104). cDNA was synthesized following the iScriptTM gDNA Clear cDNA Synthesis Kit (Bio-Rad #1725035). .. SYBR green probes (obtained from Bio-Rad) and FAM probes (obtained from iDT) targeting genes of interest were applied to evaluate gene expression under the CFX96 Touch Real-Time PCR Detection System.

    Article Title: Long-read RNA sequencing identifies non-coding isoform switching as a regulator of cell fate
    Article Snippet: .. cDNA was synthesized from 500ng of RNA by oligo-dT and random priming with iScriptTM gDNA Clear cDNA Synthesis Kit (Bio-Rad #1725035), according to the manufacturer’s instructions. .. cDNA was diluted ten times and qPCRs were performed using SYBRTM Select Master Mix (ThermoFisher Scientific #4472908) on a CFX384Touch real-timePCRdetection system (Bio-Rad).

    Article Title: Human iPSC-Derived Dorsal Root Ganglion Organoid Modeling of Chemotherapy-Induced Peripheral Neuropathy.
    Article Snippet: .. RNA was extracted from iDRGOs using TRIzol (ThermoFisher ScientificTM, Catalog No., Waltham, MA, USA), chloroform (Sigma-Aldrich, Catalog No., St. Louis, MO, USA), and isopropanol precipitation. cDNA was synthesized from RNA using iScript gDNA cDNA Synthesis Kit (BioRAD, Catalog No. 172503, Hercules, CA, USA). .. Quantitative RT-PCR was performed using iTaq Universal SYBR Green One-Step Kit (BioRAD, Catalog No. 1725150, Hercules, CA, USA).

    Quantitative RT-PCR:

    Article Title: Diaphragm-specific effects of L-citrulline in mdx mice highlight its potential as adjuvant of standard therapy in Duchenne muscular dystrophy.
    Article Snippet: 2.2.4 | Isolation of total RNA, reverse transcription and qRT-PCR Total RNA was isolated from DIA and gastrocnemius (GC) muscles after homogenization, with Trizol (10296028, Life Technologies, CA, USA) and quantified for yield and purity by spectrophotometry (ND1000 NanoDrop, Thermo Fisher Scientific). .. Variable amounts of RNA (between 1 and 2 μg) were then reverse transcribed to cDNA with iScript gDNA Clear cDNA Synthesis Kit (172-5035 Bio-Rad Laboratories, Inc., CA, USA) following manufacturer instructions. qRT-PCR was performed via the CFX384 Connect Real-Time PCR System (Bio-Rad, Hercules, CA, USA) by using PrimePCR assays SYBR® green with custom-made plates. ..

    Real-time Polymerase Chain Reaction:

    Article Title: Diaphragm-specific effects of L-citrulline in mdx mice highlight its potential as adjuvant of standard therapy in Duchenne muscular dystrophy.
    Article Snippet: 2.2.4 | Isolation of total RNA, reverse transcription and qRT-PCR Total RNA was isolated from DIA and gastrocnemius (GC) muscles after homogenization, with Trizol (10296028, Life Technologies, CA, USA) and quantified for yield and purity by spectrophotometry (ND1000 NanoDrop, Thermo Fisher Scientific). .. Variable amounts of RNA (between 1 and 2 μg) were then reverse transcribed to cDNA with iScript gDNA Clear cDNA Synthesis Kit (172-5035 Bio-Rad Laboratories, Inc., CA, USA) following manufacturer instructions. qRT-PCR was performed via the CFX384 Connect Real-Time PCR System (Bio-Rad, Hercules, CA, USA) by using PrimePCR assays SYBR® green with custom-made plates. ..

    SYBR Green Assay:

    Article Title: Diaphragm-specific effects of L-citrulline in mdx mice highlight its potential as adjuvant of standard therapy in Duchenne muscular dystrophy.
    Article Snippet: 2.2.4 | Isolation of total RNA, reverse transcription and qRT-PCR Total RNA was isolated from DIA and gastrocnemius (GC) muscles after homogenization, with Trizol (10296028, Life Technologies, CA, USA) and quantified for yield and purity by spectrophotometry (ND1000 NanoDrop, Thermo Fisher Scientific). .. Variable amounts of RNA (between 1 and 2 μg) were then reverse transcribed to cDNA with iScript gDNA Clear cDNA Synthesis Kit (172-5035 Bio-Rad Laboratories, Inc., CA, USA) following manufacturer instructions. qRT-PCR was performed via the CFX384 Connect Real-Time PCR System (Bio-Rad, Hercules, CA, USA) by using PrimePCR assays SYBR® green with custom-made plates. ..



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