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iplex buffer plus  (agena bioscience)


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    Structured Review

    agena bioscience iplex buffer plus
    Iplex Buffer Plus, supplied by agena bioscience, used in various techniques. Bioz Stars score: 96/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/iplex/iPLEX+Buffer+Plus/pm35489698-52-31-34
    Average 96 stars, based on 18 article reviews
    iplex buffer plus - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Lymphocyte-Rich Spindle Cell Thymoma
    Article Snippet: A study of 80 cases of spindle cell thymoma in which the spindle cell component was overshadowed by massive numbers of stromal lymphocytes is presented.. The patients were 38 women and 42 men, aged 8 to 81 years (mean= 54 y).. All tumors presented as an anterior mediastinal mass; 5 patients had myasthenia gravis and one had Good syndrome.

    Article Title: Interaction analysis of gene variants related to one-carbon metabolism with chronic hepatitis B infection in Chinese patients.
    Article Snippet: Funding information Foundation of Henan Charity Federation, Grant/Award Number: GDXZ2019005; Medical Science and Technology Research Project of Henan Provincial Health Commission, Grant/Award Number: SB201901045; National Natural Science Foundation of China, Grant/Award Number: NSFC 81971881; the Joint project of Medical science and Technology Research Program of Henan Province, Grant/Award Number: LHGJ20190135; the Key Scientific Research Projects Plan of Henan Higher Education Institutions, Grant/Award Number: 20A320014; the Postdoctoral Research Grant in Henan Province, Grant/Award Number: 19030009; the Provincial ministry Joint Project of Henan Provincial Health and Family Planning Commission, Grant/Award Number: SBGJ2018002 Abstract Background: The risk of chronic hepatitis B (CHB) infection is influenced by aberrant DNA methylation and altered nucleotide synthesis and repair, possibly caused by polymorphic variants in one-carbon metabolism genes.. In the present study, we investigated the relationship between polymorphisms belonging to the one-carbon metabolic pathway and CHB infection.. Methods: A case–control study using 230 CHB patients and 234 unrelated healthy controls was carried out to assess the genetic association of 24 single nucleotide polymorphisins (SNPs) determined by mass spectrometry.

    Article Title: Interaction analysis of FADS2 gene variants with chronic hepatitis B infection in Chinese patients.
    Article Snippet: Infection, Genetics and Evolution 101 (2022) 105289 The AssayDesigner3.1 software was used to designe the PCR and single-base extension primers (Table 2), and the primers were synthesized using Thermo Fisher Scientific Co.,Ltd. .. Primer mixture (Thermo, GBI), 10 × PCR Buffer (Agena, Inc), 5 U/mL HotStar Taq (Agena, Inc), dNTP mixture (Agena, Inc), shrimp alkaline phosphatase Buffer (Agena, Inc), iPLEX Termination mix (Agena, Inc), iPLEX Buffer Plus (Agena, Inc), and iPLEX Enzyme (Agena, Inc) were used for genotyping SNPs which were determined by Sequenom MassARRAY® matrix-assisted laser desorption/ ionization-time of flight mass spectrometry (MALDITOF MS) platform (Sequenom Inc., San Diego, CA, USA). .. The genotype plots of SNPs were generated by the TYPER4.0 (Agena, Inc) software.

    Amplification:

    Article Title: Lymphocyte-Rich Spindle Cell Thymoma
    Article Snippet: A study of 80 cases of spindle cell thymoma in which the spindle cell component was overshadowed by massive numbers of stromal lymphocytes is presented.. The patients were 38 women and 42 men, aged 8 to 81 years (mean= 54 y).. All tumors presented as an anterior mediastinal mass; 5 patients had myasthenia gravis and one had Good syndrome.

    Incubation:

    Article Title: A multiplex method for detection of SARS-CoV-2 variants based on MALDI-TOF mass spectrometry
    Article Snippet: Viral RNA of the three SARS-CoV-2 clinical isolates (Delta, Omicron BA.1, and Omicron BA.5) was quantified using a Qubit 4 fluorometer (Thermo Fisher Scientific Inc.). .. The SBE reaction containing 0.94 μL extension probe cocktail, 0.2 μL 10 × iPLEX Buffer Plus, 0.2 μL terminator mix, and 0.041 μL ThermoSequenase enzyme (Agena Bioscience, Inc., San Diego, CA, USA) was added to the reactions and incubated in accordance with standard conditions (95 °C for 30 s; 45 cycles, each of which included 94 °C for 5 s, followed by 5 cycles at 52 °C for 5 s and 80 °C for 5 s; 72 °C for 3 min). .. Finally, the reaction products were mixed with 11 μL nuclease-free water and fully contacted with a cationic ion exchange resin (Beijing JOHN LUNDA Technology Development Co., Ltd., Beijing, China) by rotation at 80 rpm for 40 min to absorb the cations.

    Mass Spectrometry:

    Article Title: Interaction analysis of FADS2 gene variants with chronic hepatitis B infection in Chinese patients.
    Article Snippet: Infection, Genetics and Evolution 101 (2022) 105289 The AssayDesigner3.1 software was used to designe the PCR and single-base extension primers (Table 2), and the primers were synthesized using Thermo Fisher Scientific Co.,Ltd. .. Primer mixture (Thermo, GBI), 10 × PCR Buffer (Agena, Inc), 5 U/mL HotStar Taq (Agena, Inc), dNTP mixture (Agena, Inc), shrimp alkaline phosphatase Buffer (Agena, Inc), iPLEX Termination mix (Agena, Inc), iPLEX Buffer Plus (Agena, Inc), and iPLEX Enzyme (Agena, Inc) were used for genotyping SNPs which were determined by Sequenom MassARRAY® matrix-assisted laser desorption/ ionization-time of flight mass spectrometry (MALDITOF MS) platform (Sequenom Inc., San Diego, CA, USA). .. The genotype plots of SNPs were generated by the TYPER4.0 (Agena, Inc) software.



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