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high-co-ip buffer (Active Motif)

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Active Motif high-co-ip buffer
High Co Ip Buffer, supplied by Active Motif, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ip+buffer/high+co+ip+buffer/pmc02698542-176-9-11
Average 90 stars, based on 1 article reviews

high-co-ip buffer - by Bioz Stars, 2026-09

90/100 stars

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Protease Inhibitor:

Article Title: An epigenome atlas of neural progenitors within the embryonic mouse forebrain
Article Snippet: .. Nuclei suspensions were centrifuged 500 × g for 5 min at 4 °C, the supernatant removed, and washed once with 1 mL wash buffer, centrifuged 500 × g for 5 min, and washed a final time with 1 mL 1X CUT&Tag wash buffer (from CUT&Tag IT Assay Kit, Active Motif, #53610) or 1 mL CUT&RUN 1X wash buffer (1 mL HEPES pH 7.5, 1.5 m 5 M NaCl, 12.5 uL 2 M spermidine and 47.5 mL dH 2 O with 1 Roche Complete Protease Inhibitor EDTA-Free tablet). ..

other:

Article Title: Loss of Ezh2 in the medial ganglionic eminence alters interneuron fate, cell morphology and gene expression profiles
Article Snippet: After removing supernatant, nuclei pellet was washed once with 1 mL Nuclei Wash Buffer and then with 1 mL of 1X CUT&Tag Wash Buffer (from Active Motif CUT&Tag IT Assay kit), spinning with same conditions above.

Article Title: Overexpression of parkin rescues the defective mitochondrial phenotype and the increased apoptosis of Cockayne Syndrome A cells
Article Snippet: Beads were washed 5 times with 1X IP low buffer (Active Motif, Carlsbad, US) and the immunoprecipitated proteins were eluted with the Flag peptide (Sigma-Aldrich, Saint Louis, US) in TBS (Tris 10 mM pH 7.4, NaCl 150 mM).

Article Title: Loss of Kmt2c or Kmt2d drives brain metastasis via KDM6A-dependent upregulation of MMP3.
Article Snippet: Next, 1.5 mg protein lysate was diluted up to 1 ml with low IP buffer from the Nuclear Complex Co-IP Kit (Active Motif), then 2.5 μg antibody (KDM6A; 33510S; Cell Signaling Technology) was added before incubation on a rotator at 4 °C.

Article Title: Proteomic Analyses Identify a Novel Role for EZH2 in the Initiation of Cancer Cell Drug Tolerance.
Article Snippet: Subscriber access provided by EDINBURGH UNIVERSITY LIBRARY | @ http://www.lib.ed.ac.uk is published by the American Chemical Society.. 1155 Sixteenth Street N.W., Washington, DC 20036 Published by American Chemical Society.. Copyright © American Chemical Society.

Immunoprecipitation:

Article Title: Cockayne syndrome group A and ferrochelatase finely tune ribosomal gene transcription and its response to UV irradiation
Article Snippet: Total extracts from 5 × 10 7 cells were incubated overnight at 4°C with anti-Flag M2 Affinity Gel (Sigma-Aldrich). .. Beads were washed five times with 1× IP low buffer (Active Motif) and the immunoprecipitated proteins were eluted with the Flag peptide (Sigma-Aldrich) in TBS (Tris 10 mM pH 7.4, NaCl 150 mM). .. Eluates were further immunoprecipitated with anti-HA agarose affinity resin (Sigma-Aldrich) overnight at 4°C.