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Santa Cruz Biotechnology mekk2

FIG. 1. A schematic drawing illustrates the four signaling pathways utilized by TGFb to affect cellular function. This diagram was prepared based on reviews [18, 19, 21]. Original articles that describe the discovery of the key molecules depicted in these pathways can be found in these reviews. MEKKs, MAP/ERK kinase kinases, which include MEKK1, <t>MEKK2,</t> MEKK3, and others; JNK, c-Jun NH2-terminal kinase also known as Jun kinase or stress-activated protein kinase, SAPK; JNKK, c-Jun NH2-terminal kinase kinase; MKK3, MAP kinase kinase 3; MEK1/2, MAP/ERK kinase 1 and MAP/ERK kinase 2; ERK1/2, extracellular signal-regulated kinase 1 and extracellular signal-regulated kinase 2.

Mekk2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/interect+function/MEK+kinase-2+Antibody/pm12606350-82-9-33
Average 93 stars, based on 35 article reviews

mekk2 - by Bioz Stars, 2026-09

93/100 stars

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1) Product Images from "Transforming growth factor beta3 regulates the dynamics of Sertoli cell tight junctions via the p38 mitogen-activated protein kinase pathway."

Article Title: Transforming growth factor beta3 regulates the dynamics of Sertoli cell tight junctions via the p38 mitogen-activated protein kinase pathway.

Journal: Biology of reproduction

doi: 10.1095/biolreprod.102.011387

Figure Legend Snippet: FIG. 1. A schematic drawing illustrates the four signaling pathways utilized by TGFb to affect cellular function. This diagram was prepared based on reviews [18, 19, 21]. Original articles that describe the discovery of the key molecules depicted in these pathways can be found in these reviews. MEKKs, MAP/ERK kinase kinases, which include MEKK1, MEKK2, MEKK3, and others; JNK, c-Jun NH2-terminal kinase also known as Jun kinase or stress-activated protein kinase, SAPK; JNKK, c-Jun NH2-terminal kinase kinase; MKK3, MAP kinase kinase 3; MEK1/2, MAP/ERK kinase 1 and MAP/ERK kinase 2; ERK1/2, extracellular signal-regulated kinase 1 and extracellular signal-regulated kinase 2.

Techniques Used: Protein-Protein interactions, Cell Function Assay

FIG. 4. Relative expression of the TGFb- upstream signal transducers in Sertoli and germ cells isolated from 20-day-old rat testes. A) Semiquantitative RT-PCR was performed to assess the steady-state mRNA levels of Smad2, Cdc42, Rac2, MEKK2, and N-Ras in Sertoli and germ cells. Im- munoblots were performed in parallel ex- periments to assess the relative protein levels of these signal transducers in Sertoli and germ cells using the corresponding speciﬁc antibodies (see Materials and Methods). (C). B) This ﬁgure shows the corresponding densitometrically scanned results using autoradiograms or immuno- blots such as those shown in A and C. Re- sults are expressed as mean 6 SD using three batches of cells from three different experiments normalized against S16. Each experiment had triplicate cultures. These analyses revealed that results of immuno- blots are consistent with the RT-PCR data. Statistical analysis was performed by Stu- dent t-test comparing germ cells with the corresponding Sertoli cells, which was ar- bitrarily set at one. *, Signiﬁcantly different from Sertoli cells by Student t-test, P , 0.05; **, signiﬁcantly different from Sertoli cells by Student t-test, P , 0.01.

Figure Legend Snippet: FIG. 4. Relative expression of the TGFb- upstream signal transducers in Sertoli and germ cells isolated from 20-day-old rat testes. A) Semiquantitative RT-PCR was performed to assess the steady-state mRNA levels of Smad2, Cdc42, Rac2, MEKK2, and N-Ras in Sertoli and germ cells. Im- munoblots were performed in parallel ex- periments to assess the relative protein levels of these signal transducers in Sertoli and germ cells using the corresponding speciﬁc antibodies (see Materials and Methods). (C). B) This ﬁgure shows the corresponding densitometrically scanned results using autoradiograms or immuno- blots such as those shown in A and C. Re- sults are expressed as mean 6 SD using three batches of cells from three different experiments normalized against S16. Each experiment had triplicate cultures. These analyses revealed that results of immuno- blots are consistent with the RT-PCR data. Statistical analysis was performed by Stu- dent t-test comparing germ cells with the corresponding Sertoli cells, which was ar- bitrarily set at one. *, Signiﬁcantly different from Sertoli cells by Student t-test, P , 0.05; **, signiﬁcantly different from Sertoli cells by Student t-test, P , 0.01.

Techniques Used: Expressing, Isolation, Reverse Transcription Polymerase Chain Reaction, Western Blot

FIG. 5. Developmental regulation of the steady-state mRNA levels of the TGFb up- stream signal transducers in Sertoli (A, B) and germ (C, D) cells. RT-PCR was per- formed to assess the steady-state mRNA levels of Smad2, Cdc42, Rac2, MEKK2, and N-Ras in Sertoli cells (A) and germ cells (C) during maturation. B, D) The cor- responding densitometrically scanned re- sults using autoradiograms such as those shown in A and C. Results are expressed as mean 6 SD using two batches of cells normalized against S16 from two different experiments. Each experiment had tripli- cate cultures. ns, Not signiﬁcantly different from cultures isolated from rats at 20 days of age in B and 5 or 10 days of age in D, which was arbitrarily set at one, by Stu- dent t-test; *, signiﬁcantly different by Stu- dent t-test, P , 0.05; **, signiﬁcantly dif- ferent by Student t-test, P , 0.01; nd, not detectable.

Figure Legend Snippet: FIG. 5. Developmental regulation of the steady-state mRNA levels of the TGFb up- stream signal transducers in Sertoli (A, B) and germ (C, D) cells. RT-PCR was per- formed to assess the steady-state mRNA levels of Smad2, Cdc42, Rac2, MEKK2, and N-Ras in Sertoli cells (A) and germ cells (C) during maturation. B, D) The cor- responding densitometrically scanned re- sults using autoradiograms such as those shown in A and C. Results are expressed as mean 6 SD using two batches of cells normalized against S16 from two different experiments. Each experiment had tripli- cate cultures. ns, Not signiﬁcantly different from cultures isolated from rats at 20 days of age in B and 5 or 10 days of age in D, which was arbitrarily set at one, by Stu- dent t-test; *, signiﬁcantly different by Stu- dent t-test, P , 0.05; **, signiﬁcantly dif- ferent by Student t-test, P , 0.01; nd, not detectable.

Techniques Used: Reverse Transcription Polymerase Chain Reaction, Isolation

FIG. 6. Changes in the steady-state mRNA levels of TGFb upstream signal transducers in the testis during develop- ment. A) Semiquantitative RT-PCR was performed to assess the steady-state mRNA levels of Smad2, Cdc42, Rac2, MEKK2, and N-Ras in testes during maturation. B) This panel shows the corresponding densi- tometrically scanned results using autora- diograms such as those shown in A. Re- sults are expressed as mean 6 SD using testes from three different rats normalized against S16. ns, Not signiﬁcantly different from rats at 5 days of age, which was arbi- trarily set at one, by Student t-test; *, signif- icantly different by Student t-test, P , 0.05; **, signiﬁcantly different by Student t-test, P , 0.01.

Figure Legend Snippet: FIG. 6. Changes in the steady-state mRNA levels of TGFb upstream signal transducers in the testis during develop- ment. A) Semiquantitative RT-PCR was performed to assess the steady-state mRNA levels of Smad2, Cdc42, Rac2, MEKK2, and N-Ras in testes during maturation. B) This panel shows the corresponding densi- tometrically scanned results using autora- diograms such as those shown in A. Re- sults are expressed as mean 6 SD using testes from three different rats normalized against S16. ns, Not signiﬁcantly different from rats at 5 days of age, which was arbi- trarily set at one, by Student t-test; *, signif- icantly different by Student t-test, P , 0.05; **, signiﬁcantly different by Student t-test, P , 0.01.

Techniques Used: Reverse Transcription Polymerase Chain Reaction

FIG. 8. Change in the steady-state mRNA and protein levels of the TGFb upstream signal transducers when the Sertoli cell TJ barrier was assembled in vitro in the ab- sence (control) and presence (test) of TGFb3. Sertoli cells (0.5 3 106 cells/cm2) cultured on Matrigel-coated dishes in the absence (A, C, E) or presence (B, D, F) of TGFb3 (3 ng/ml) were terminated by RNA STAT-60 or lysed in SDS sample buffer at speciﬁed time points. Cell lysates (;200 mg protein) from each time point were re- solved by SDS-PAGE under reducing con- ditions using 10% T SDS-polyacrylamide gels. Immunoblotting was performed to as- sess changes in the levels of Smad2, Cdc42, Rac2, N-Ras, and MEKK2 in the absence (A, E) and presence (B, F) of re- combinant TGFb3 (3 ng/ml). RT-PCR was performed to assess changes in the MEKK2 steady-state mRNA level in the absence (C) or presence (D) of recombinant TGFb3 (3 ng/ml). G, H) Corresponding densito- metrically scanned results using autoradio- grams and immunoblots shown in C–F. Results are expressed as mean 6 SD from three separate experiments using different batches of cells and normalized against S16. Each time point had duplicate cul- tures. ns, Not signiﬁcantly different by AN- OVA, in which each sample at a given time point was compared with samples of all other time points within the same ex- perimental group; *, signiﬁcantly different by ANOVA, P , 0.01; D, days.

Figure Legend Snippet: FIG. 8. Change in the steady-state mRNA and protein levels of the TGFb upstream signal transducers when the Sertoli cell TJ barrier was assembled in vitro in the ab- sence (control) and presence (test) of TGFb3. Sertoli cells (0.5 3 106 cells/cm2) cultured on Matrigel-coated dishes in the absence (A, C, E) or presence (B, D, F) of TGFb3 (3 ng/ml) were terminated by RNA STAT-60 or lysed in SDS sample buffer at speciﬁed time points. Cell lysates (;200 mg protein) from each time point were re- solved by SDS-PAGE under reducing con- ditions using 10% T SDS-polyacrylamide gels. Immunoblotting was performed to as- sess changes in the levels of Smad2, Cdc42, Rac2, N-Ras, and MEKK2 in the absence (A, E) and presence (B, F) of re- combinant TGFb3 (3 ng/ml). RT-PCR was performed to assess changes in the MEKK2 steady-state mRNA level in the absence (C) or presence (D) of recombinant TGFb3 (3 ng/ml). G, H) Corresponding densito- metrically scanned results using autoradio- grams and immunoblots shown in C–F. Results are expressed as mean 6 SD from three separate experiments using different batches of cells and normalized against S16. Each time point had duplicate cul- tures. ns, Not signiﬁcantly different by AN- OVA, in which each sample at a given time point was compared with samples of all other time points within the same ex- perimental group; *, signiﬁcantly different by ANOVA, P , 0.01; D, days.

Techniques Used: In Vitro, Control, Cell Culture, SDS Page, Western Blot, Reverse Transcription Polymerase Chain Reaction, Recombinant

Incubation:

Article Title: Acute physical exercise increases APPL1/PI3K signaling in the hypothalamus of lean mice.
Article Snippet: Adiponectin is an adipokine that acts in the control of energy homeostasis.. The adaptor protein containing the pleckstrin homology domain, phosphotyrosine-binding domain, and leucine zipper motif 1 (APPL1) is a key protein in the adiponectin signaling.. The APPL1 mediates a positive effect on the insulin signaling through the interaction with the phosphoinositide 3-kinase (PI3K).

Immunohistochemistry:

Article Title: Mutually exclusive acetylation and ubiquitylation of the splicing factor SRSF5 control tumor growth
Article Snippet: All antibodies were purchased as follows: Anti-SRSF5 for IB (ab67175, 1:400 dilution), anti-Smurf1 for IB (ab117552, 1:500) were purchased from Abcam. .. Anti-MEKK2 (sc1088, N-19, 1:200), anti-Smurf1 for IHC (sc25510, H60, 1:80), anti-Tip60 (N17, 1:100), anti-GST (sc-374171, A-6, 1:1000), anti-His (sc8036, H3, 1:300), anti-actin (sc-1616, 1:1000), Normal IgG (sc-2025, 1: 200) were purchased from Santa Cruz. .. Anti-HDAC1 (10E2, 5356), anti-Ub (P4D1, 3936), anti-AMPKα1/2 (2535 S, 1:1000), anti-phospho-AMPKα1/2 (Thr172) (5832S, 1:500), anti-ACC (3662S, 1:500), anti-phospho-ACC (Ser79) (3661S, 1:500), anti-S6K (2708, 1:400), anti-phospho-S6K(T389) (9234, 1:400), anti-mTOR (2983, 1:500) and anti-acetylated lysine antibody (9441, 1:400) were purchased from Cell Signaling.

other:

Article Title: Platelet-derived growth factor (PDGF)-induced activation of Erk5 MAP-kinase is dependent on Mekk2, Mek1/2, PKC and PI3-kinase, and affects BMP signaling.
Article Snippet: Platelet-derived growth factor (PDGF)-induced activation of Erk5 MAPkinase is dependent on Mekk2, Mek1/2, PKC and PI3-kinase, and affects BMP signaling Maria Tsioumpekou, Natalia Papadopoulos, Fatima Burovic, Carl-Henrik Heldin, Johan Lennartsson PII: S0898-6568(16)30147-4 DOI: doi: 10.1016/j.cellsig.2016.06.013 Reference: CLS 8714 To appear in: Cellular Signalling Received date: 8 February 2016 Revised date: 16 June 2016 Accepted date: 16 June 2016 Please cite this article as: Maria Tsioumpekou, Natalia Papadopoulos, Fatima Burovic, Carl-Henrik Heldin, Johan Lennartsson, Platelet-derived growth factor (PDGF)-induced activation of Erk5 MAP-kinase is dependent on Mekk2, Mek1/2, PKC and PI3-kinase, and affects BMP signaling, Cellular Signalling (2016), doi: 10.1016/j.cellsig.2016.06.013 This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form.

Western Blot:

Article Title: MicroRNAs-143 and -145 induce epithelial to mesenchymal transition and modulate the expression of junction proteins
Article Snippet: Firefly and Renilla Luciferase activities were measured 48 h upon transfection, with the Dual-Glo Luciferase Assay system (Promega, Madison, WI, USA) and a 96 microplates GloMax luminometer (Promega). .. Western blots Total protein extracts were obtained and Western blots performed as described in Schiavone et al. 51 The following antibodies were used: N-cadherin (Abcam, Cambridge, UK); E-cadherin, β -catenin (BD, Franklin Lakes, NJ, USA); Snail, pSMAD2, SMAD2, pSMAD3-1, SMAD3, pERK1/2, ERK1/2, pMEK1/2, MEK1/2, Creb1 (Cell Signaling Technology, Danvers, MA, USA); α -SMA, α -tubulin (Sigma-Aldrich); ZO1, ZO3, Occludin (Thermo Fisher Scientific); SMAD4, Egr1, c-Fos, MEKK2, TGIF, Actin (Santa Cruz Biotechnology, Santa Cruz, CA, USA); GAPDH (Merck-Millipore); Vinculin and GFP (home made). .. Western blot chemilumiscent signals were acquired with a ChemidocTouch and analyzed with the ImageLab software (BioRad).

Review