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intercept protein free blocking buffer  (LI-COR)


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    Structured Review

    LI-COR intercept protein free blocking buffer
    Intercept Protein Free Blocking Buffer, supplied by LI-COR, used in various techniques. Bioz Stars score: 98/100, based on 70 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/intercept+blocking+buffer/Intercept+(TBS)+Protein-Free+Blocking+Buffer/pm42103232-281-24-27
    Average 98 stars, based on 70 article reviews
    intercept protein free blocking buffer - by Bioz Stars, 2026-10
    98/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Reprogramming breast cancer cells to breast cancer stem cell- like by the POU1F1 transcription factor
    Article Snippet: Cell extracts (30 μg) were denatured at 95°C, subjected to SDS-PAGE, and transferred to PVDF membranes (Merck Millipore, Burlington, USA). .. After blocking with Intercept Protein-Free Blocking buffer (LI-COR Biotechnology, Lincoln, USA), membranes were incubated overnight at 4°C with anti-POU1F1 antibody (Sigma), anti-GPDH, and anti-β-actin (Santacruz), anti-STAT3, anti-pSTAT3, anti-ERK1/2, anti-pERK1/2, anti-AKT, and anti-pAKT (Cell Signalling, Danvers, USA) antibodies for their expression and phosphorylation. .. A fluorescent secondary antibody was then applied, and detection was performed using the Li-Cor Odyssey fluorescence system at 700 and 800 nm wavelengths.

    Article Title: Discovery of a potent anti-Zika virus benzamide series targeting the viral protein NS4B
    Article Snippet: .. After blocking for one hour with Intercept Protein-Free blocking buffer (Li-Cor), cells were incubated with MWAC-4163 in PBS with 10% blocking buffer for 30 minutes. .. After extensive washing, cells were analyzed on an Accuri C6 flow cytometer (BD Sciences) using channel 1 for FAM (ex488 nm/em533) and channel 4 (ex640 nm/em675LP) for miniRFP.

    Article Title: Immunoblot-based activity assay for heme-containing histidine kinases
    Article Snippet: .. Membranes from Dot Blotting and Western Blotting were rehydrated with TBS and blocked with Intercept (TBS) protein-free blocking buffer (LI-COR) for 60 min at room temperature while rocking. .. Primary antibody solution was prepared by diluting rabbit anti-thiophosphoester antibody (Abcam, ab92570) in Intercept T20 (TBS) protein-free antibody diluent.

    Article Title: MD-4251: A First-in-Class Oral MDM2 Degrader Inducing Complete Tumor Regression with Single-Dose Administration.
    Article Snippet: MDM2 is a key negative regulator of the tumor suppressor p53 and an attractive target for cancer therapy.. We report the discovery of MD-4251, the first orally efficacious MDM2 degrader developed using PROTAC technology.. MD-4251 induces potent and rapid MDM2 degradation in RS4;11 cells (DC50 = 0.2 nM; Dmax = 96% at 2 h), leading to robust p53 activation.

    Article Title: Identifying transcription factors controlling the basal expression of human MRP4 highlights a substantial role for Sp1.
    Article Snippet: .. After blocking in protein-free blocking buffer (Intercept , LI-COR Biosciences GmbH), membranes were incubated with the rat anti-MRP4 monoclonal antibody (M4-I-10, Enzo Life Sciences, L€orrach, Germany, 1 : 1000) and the rabbit polyclonal anti-Sp1 IgG (Merck KGaA, 1 : 1000). bactin was stained as a loading control (ACTBD11B7 antibody; Santa Cruz Biotechnology; 1 : 1000). .. IRDye fluorescence labeled secondary antibodies (LI-COR Biosciences GmbH) were used for detection.

    Article Title: GlycoRNA-L and glycoRNA-S mediate human monocyte adhesion via binding to Siglec-5.
    Article Snippet: It was recently reported that RNAs can be glycosylated, and such glycosylated RNAs (referred to as glycoRNAs) are located on the outer cell surface.. We here reported that there are two forms of glycoRNAs, named as glycoRNA-L and glycoRNA-S, robustly expressed in human monocytes.. We verified that the glycoRNA-S specifically detected in human monocytes is synthesized by enzyme-catalyzed conjugation, but not artificial products of labelling probe.

    Article Title: Geranylgeraniol promotes osteoblast differentiation and inhibits osteoclastogenesis through MAPK and nuclear receptor signaling.
    Article Snippet: Samples were separated using SDS-PAGE and transferred onto Immobilon-P PVDF membranes (Millipore, Billerica, MA, USA) for western blot analysis. .. The membrane was blocked with 5% skim milk in TBST (137mM NaCl, 2.68 mM KCl, 25 mM Tris pH 7.4 0.1% Tween 20), or intercept-protein-free blocking buffer (LICORbio), incubated with anti-mouse antibodies for 2‒12 h, and then incubated with horseradishperoxidase-conjugated anti-rabbit antibody. .. Protein bands were detected using Clarity Western ECL Substrate (Bio-Rad, Hercules, CA, USA) or ImmunoStar LD (Fujifilm Wako Pure Chemical), and analyzed using a ChemiDoc MP imaging system (Bio-Rad) or C-DiGit Blot Scanner (LICORbio).

    Article Title: Treatment of acute myeloid leukemia models by targeting a cell surface RNA-binding protein.
    Article Snippet: After transfer, the membrane was rinsed with 1× PBS and dried on Whatman paper (GE Healthcare). .. Dried membranes were rehydrated in Intercept Protein-Free Blocking Buffer, TBS (LI-COR Biosciences) for 30 min at 25 °C. .. After blocking, the membranes were stained using streptavidin IR800 (LI-COR Biosciences) diluted 1:5,000 in Intercept blocking buffer for 30 min at 25 °C.

    Incubation:

    Article Title: Reprogramming breast cancer cells to breast cancer stem cell- like by the POU1F1 transcription factor
    Article Snippet: Cell extracts (30 μg) were denatured at 95°C, subjected to SDS-PAGE, and transferred to PVDF membranes (Merck Millipore, Burlington, USA). .. After blocking with Intercept Protein-Free Blocking buffer (LI-COR Biotechnology, Lincoln, USA), membranes were incubated overnight at 4°C with anti-POU1F1 antibody (Sigma), anti-GPDH, and anti-β-actin (Santacruz), anti-STAT3, anti-pSTAT3, anti-ERK1/2, anti-pERK1/2, anti-AKT, and anti-pAKT (Cell Signalling, Danvers, USA) antibodies for their expression and phosphorylation. .. A fluorescent secondary antibody was then applied, and detection was performed using the Li-Cor Odyssey fluorescence system at 700 and 800 nm wavelengths.

    Article Title: Discovery of a potent anti-Zika virus benzamide series targeting the viral protein NS4B
    Article Snippet: .. After blocking for one hour with Intercept Protein-Free blocking buffer (Li-Cor), cells were incubated with MWAC-4163 in PBS with 10% blocking buffer for 30 minutes. .. After extensive washing, cells were analyzed on an Accuri C6 flow cytometer (BD Sciences) using channel 1 for FAM (ex488 nm/em533) and channel 4 (ex640 nm/em675LP) for miniRFP.

    Article Title: MD-4251: A First-in-Class Oral MDM2 Degrader Inducing Complete Tumor Regression with Single-Dose Administration.
    Article Snippet: MDM2 is a key negative regulator of the tumor suppressor p53 and an attractive target for cancer therapy.. We report the discovery of MD-4251, the first orally efficacious MDM2 degrader developed using PROTAC technology.. MD-4251 induces potent and rapid MDM2 degradation in RS4;11 cells (DC50 = 0.2 nM; Dmax = 96% at 2 h), leading to robust p53 activation.

    Article Title: Identifying transcription factors controlling the basal expression of human MRP4 highlights a substantial role for Sp1.
    Article Snippet: .. After blocking in protein-free blocking buffer (Intercept , LI-COR Biosciences GmbH), membranes were incubated with the rat anti-MRP4 monoclonal antibody (M4-I-10, Enzo Life Sciences, L€orrach, Germany, 1 : 1000) and the rabbit polyclonal anti-Sp1 IgG (Merck KGaA, 1 : 1000). bactin was stained as a loading control (ACTBD11B7 antibody; Santa Cruz Biotechnology; 1 : 1000). .. IRDye fluorescence labeled secondary antibodies (LI-COR Biosciences GmbH) were used for detection.

    Article Title: Geranylgeraniol promotes osteoblast differentiation and inhibits osteoclastogenesis through MAPK and nuclear receptor signaling.
    Article Snippet: Samples were separated using SDS-PAGE and transferred onto Immobilon-P PVDF membranes (Millipore, Billerica, MA, USA) for western blot analysis. .. The membrane was blocked with 5% skim milk in TBST (137mM NaCl, 2.68 mM KCl, 25 mM Tris pH 7.4 0.1% Tween 20), or intercept-protein-free blocking buffer (LICORbio), incubated with anti-mouse antibodies for 2‒12 h, and then incubated with horseradishperoxidase-conjugated anti-rabbit antibody. .. Protein bands were detected using Clarity Western ECL Substrate (Bio-Rad, Hercules, CA, USA) or ImmunoStar LD (Fujifilm Wako Pure Chemical), and analyzed using a ChemiDoc MP imaging system (Bio-Rad) or C-DiGit Blot Scanner (LICORbio).

    Expressing:

    Article Title: Reprogramming breast cancer cells to breast cancer stem cell- like by the POU1F1 transcription factor
    Article Snippet: Cell extracts (30 μg) were denatured at 95°C, subjected to SDS-PAGE, and transferred to PVDF membranes (Merck Millipore, Burlington, USA). .. After blocking with Intercept Protein-Free Blocking buffer (LI-COR Biotechnology, Lincoln, USA), membranes were incubated overnight at 4°C with anti-POU1F1 antibody (Sigma), anti-GPDH, and anti-β-actin (Santacruz), anti-STAT3, anti-pSTAT3, anti-ERK1/2, anti-pERK1/2, anti-AKT, and anti-pAKT (Cell Signalling, Danvers, USA) antibodies for their expression and phosphorylation. .. A fluorescent secondary antibody was then applied, and detection was performed using the Li-Cor Odyssey fluorescence system at 700 and 800 nm wavelengths.

    Phospho-proteomics:

    Article Title: Reprogramming breast cancer cells to breast cancer stem cell- like by the POU1F1 transcription factor
    Article Snippet: Cell extracts (30 μg) were denatured at 95°C, subjected to SDS-PAGE, and transferred to PVDF membranes (Merck Millipore, Burlington, USA). .. After blocking with Intercept Protein-Free Blocking buffer (LI-COR Biotechnology, Lincoln, USA), membranes were incubated overnight at 4°C with anti-POU1F1 antibody (Sigma), anti-GPDH, and anti-β-actin (Santacruz), anti-STAT3, anti-pSTAT3, anti-ERK1/2, anti-pERK1/2, anti-AKT, and anti-pAKT (Cell Signalling, Danvers, USA) antibodies for their expression and phosphorylation. .. A fluorescent secondary antibody was then applied, and detection was performed using the Li-Cor Odyssey fluorescence system at 700 and 800 nm wavelengths.

    Western Blot:

    Article Title: Immunoblot-based activity assay for heme-containing histidine kinases
    Article Snippet: .. Membranes from Dot Blotting and Western Blotting were rehydrated with TBS and blocked with Intercept (TBS) protein-free blocking buffer (LI-COR) for 60 min at room temperature while rocking. .. Primary antibody solution was prepared by diluting rabbit anti-thiophosphoester antibody (Abcam, ab92570) in Intercept T20 (TBS) protein-free antibody diluent.

    Staining:

    Article Title: Identifying transcription factors controlling the basal expression of human MRP4 highlights a substantial role for Sp1.
    Article Snippet: .. After blocking in protein-free blocking buffer (Intercept , LI-COR Biosciences GmbH), membranes were incubated with the rat anti-MRP4 monoclonal antibody (M4-I-10, Enzo Life Sciences, L€orrach, Germany, 1 : 1000) and the rabbit polyclonal anti-Sp1 IgG (Merck KGaA, 1 : 1000). bactin was stained as a loading control (ACTBD11B7 antibody; Santa Cruz Biotechnology; 1 : 1000). .. IRDye fluorescence labeled secondary antibodies (LI-COR Biosciences GmbH) were used for detection.

    Control:

    Article Title: Identifying transcription factors controlling the basal expression of human MRP4 highlights a substantial role for Sp1.
    Article Snippet: .. After blocking in protein-free blocking buffer (Intercept , LI-COR Biosciences GmbH), membranes were incubated with the rat anti-MRP4 monoclonal antibody (M4-I-10, Enzo Life Sciences, L€orrach, Germany, 1 : 1000) and the rabbit polyclonal anti-Sp1 IgG (Merck KGaA, 1 : 1000). bactin was stained as a loading control (ACTBD11B7 antibody; Santa Cruz Biotechnology; 1 : 1000). .. IRDye fluorescence labeled secondary antibodies (LI-COR Biosciences GmbH) were used for detection.

    Membrane:

    Article Title: Geranylgeraniol promotes osteoblast differentiation and inhibits osteoclastogenesis through MAPK and nuclear receptor signaling.
    Article Snippet: Samples were separated using SDS-PAGE and transferred onto Immobilon-P PVDF membranes (Millipore, Billerica, MA, USA) for western blot analysis. .. The membrane was blocked with 5% skim milk in TBST (137mM NaCl, 2.68 mM KCl, 25 mM Tris pH 7.4 0.1% Tween 20), or intercept-protein-free blocking buffer (LICORbio), incubated with anti-mouse antibodies for 2‒12 h, and then incubated with horseradishperoxidase-conjugated anti-rabbit antibody. .. Protein bands were detected using Clarity Western ECL Substrate (Bio-Rad, Hercules, CA, USA) or ImmunoStar LD (Fujifilm Wako Pure Chemical), and analyzed using a ChemiDoc MP imaging system (Bio-Rad) or C-DiGit Blot Scanner (LICORbio).



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