buffer (LI-COR)

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LI-COR buffer
Buffer, supplied by LI-COR, used in various techniques. Bioz Stars score: 98/100, based on 1127 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/intercept+blocking+buffer/Intercept+(PBS)+Blocking+Buffer/pm41932441-296-22-23
Average 98 stars, based on 1127 article reviews

buffer - by Bioz Stars, 2026-11

98/100 stars

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Incubation:

Article Title: MEK interactions tune RAF kinase sensitivity to conformation-selective inhibition.
Article Snippet: .. Samples were then incubated with 125 mM glycine in 1× PBS for 5 min, followed by blocking with Intercept PBS blocking buffer (LI-COR Biosciences) for 1 h at 37 °C. .. Then, p44/42 MAPK (ERK1/2) (3A7) mouse mAb (Cell Signaling Technology) and phospho-p44/42 MAPK (ERK1/2) D13.14.4E XP rabbit mAb (Cell Signaling Technology) were diluted in Intercept PBS blocking buffer (LI-COR Biosciences) and added to the samples, followed by incubation for 1 h at room temperature.

Article Title: Prohibitin-1 cysteine-69 plays a sex-dependent role in fatty acid metabolism.
Article Snippet: Tissue lysates or immunoprecipitates were separated on 4%-20% gradient SDS-PAGE gels and transferred to Immobilon-FL membranes (MilliporeSigma, #IPFL00010). .. Membranes were blocked with Odyssey Blocking Buffer (LI-COR, #927-70001) and incubated with anti-PHB or anti-CD36 (R&D Systems, #AF1955) antibodies. .. Immunoreactivity was detected using the Odyssey CLx Imaging System (LI-COR).

Article Title: Mutation of a single cysteine in CaMKIIδ protects the heart from ischemia-reperfusion Injury
Article Snippet: 20 μg (HEK293) or 50ug (Heart) of protein was loaded onto each lane, and SDS-PAGE was performed on Novex 10 to 20% Tris-glycine gel (Thermo Fisher Scientific XP10200BOX) in Novex Tris-glycine SDS running buffer (Invitrogen LC2675) at a maximum 125 V for 2 h. Proteins were transferred to nitrocellulose membrane using the Trans-Blot Turbo Mini 0.2 μm Nitrocellulose Transfer (BioRad 1704158) at 25 V, 1.3–2.5 A, 7 minutes using the BioRad Trans-Blot Turbo system. .. Membranes were blocked for 30 min with blocking buffer (Li-Cor 927-70001), followed by incubation in blocking buffer supplemented with 0.05% Tween 20 and 1:10,000 diluted primary antibodies overnight on a rocking platform at 4 C. The next day, incubation was continued for 1 more hour at room temperature and then washed with 1× PBS, pH 7.4, twice for 10 min, followed by incubation in blocking buffer (0.05% Tween 20) supplemented with a 1:25,000 dilution of LICOR IRDye 800CW Goat anti-Rabbit IgG Secondary Antibody (LICOR 92632211) for 20 min at room temperature on a rocking platform. .. Membranes were then washed with PBS for 15 min with a change of buffer every 5 min. Quantitative Western blot images were obtained by scanning the membrane on a Li-Cor Odyssey infrared imaging system according to the manufacturer’s instructions.

Article Title: BAG2 Condensates Couple Proteostasis to CD8 + T Cell Surveillance
Article Snippet: .. After overnight incubation (4 C) with primary antibody – rabbit anti-BAG2 1:2000 (AbCam), mouse anti MHC-I, 1:1000 (Santa Cruz Biotechnology), rabbit anti PSMB8, 1:1000 (Invitrogen), rabbit anti-PHF-1, 1:50 (PeterDavis), mouse anti tau5, 1:500 (Thermofisher, Cat#AHB0042) – the blots were washed 2x in Tween 20-TBS (TBS-T) then incubated with IRDye secondary antibodies (1:5000 dilution in Intercept Blocking Buffer, Li-cor, Cat# 927-70001) for 1 h at RT. .. The blots were then washed 2x in TBS-T and imaged using a digital fluorescent imaging system (ChemiDoc MP Imaging System, BioRad).

Article Title: Insulin Receptor Isoform Pattern in Aged Normal and Alzheimer's Human Cortex
Article Snippet: The protein was transferred onto methanol‐wetted polyvinylidene fluoride (PVDF) membranes (cat. no. GE10600023, Amersham Biosciences) for 90 min at 100 V in a Mini Trans‐Blot Cell module (Bio‐Rad) with ice‐cold NuPAGE transfer buffer (cat. no. NP0006, Invitrogen) containing 20% methanol. .. After protein transfer, the membranes were incubated for an hour with Intercept blocking buffer (cat. no. 927‐70001, LICORbio). .. Each membrane was incubated with a mouse anti‐β‐actin antibody as a loading control (cat. no. ab6276, Abcam; 1:1000) and one of three rabbit anti‐IRβ antibodies (Novus Biologicals, NBP2‐12793, 1:500; Invitrogen, PA5‐27334, 1:500; or Abbiotec, 250 724, 1:300) in a solution of 5% bovine serum albumin (BSA) in tris‐buffered saline with 0.1% Tween 20 (TBS‐T) at 4°C overnight with agitation.

Blocking Assay:

Article Title: A Lenalidomide-Inducible Suicide Switch for Gene- and Cell-Based Therapy
Article Snippet: Next, 30 μg of proteins were separated with 10% Bis-Tris gels (Invitrogen, NW00105BOX) then transferred onto nitrocellulose membranes (Thermo Scientific, 88018) as per the manufacturer’s instruction. .. After the transfer, membranes were blocked by a blocking buffer (LICORbio, 927-70001) and then stained using primary antibodies (1:1000-1:5000 dilution) followed by secondary antibodies (1:3000 dilutions). .. The blots were developed using a near-infrared (NIR) fluorescence imaging system (Odyssey CLx, LICORbio).

Article Title: KAT6A is essential for developmental control gene expression in neural stem and progenitor cells.
Article Snippet: Acid extracted proteins were run on a 4–12% Bis–Tris gel (NP0322; Thermo Fisher Scientific) and transferred onto nitrocellulose membranes (926–31090; LI-COR Biosciences) for fluorescent detection. .. Membranes were blocked for 1 h at RT on a roller in blocking buffer for fluorescent detection (Intercept [PBS], 927–70001; LI-COR) and probed with antibodies against histones, namely Anti-H3K9ac (13001; Epicypher), Anti-H3K14ac (Ab53946; Abcam), Anti-H3K23ac (07–355; Millipore) and Pan-H3 (10799; Abcam) O/N at 4°C on a roller. .. Membranes were washed in PBS + 0.1% Tween-20 (P1379; Sigma-Aldrich) and incubated with secondary antibodies (Anti-mouse IRDye 800, 926–32210; Anti-Rabbit IRDye 680, 926–68071; both LI-COR Biosciences) for 1 h at RT on a roller.

Article Title: Predicting Pain Using a Data-Driven Agent-Based Model of the Bilateral Central Amygdala
Article Snippet: 10 μg of each sample was separated on a 12.5% NuPAGE gel (Thermo Scientific Cat WBT41212BOX) then transferred to nitrocellulose membrane. .. Membranes were blocked in Odyssey blocking buffer (Licor Cat 927-70001) for 1 hr then co-incubated with mouse anti-pERK1/2 (1:1,000, Cell Signaling Cat: 9106L) and rabbit anti-ERK1/2 primary antibodies (1:1,000, Cell Signaling Cat: 9102L; in 0.1% Tween 20 in Odyssey blocking buffer) for 1 hr. .. Blots were rinsed with 0.1% Tween 20 in TBS (TTBS) then incubated with goat anti-mouse Alexa 680 (1:20,000, Molecular Probes Cat: A-21058) and goat-anti rabbit IR 800 secondary antibodies (1:20,000, Rockland Cat: 11-132-002; in 0.1% Tween 20 in Odyssey blocking buffer) for 1 hr.

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