anti gsdmd antibody (Proteintech)
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Proteintech
anti gsdmd antibody
Anti Gsdmd Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 584 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/immunohistochemistry+microarray+based+expression+data/GSDMD+Antibody/pmc11492562-54-0-2
Average 96 stars, based on 584 article reviews
Anti Gsdmd Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 584 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/immunohistochemistry+microarray+based+expression+data/GSDMD+Antibody/pmc11492562-54-0-2
Average 96 stars, based on 584 article reviews
anti gsdmd antibody - by Bioz Stars,
2026-10
96/100 stars
Images
1) Product Images from "Gasdermin D promotes development of intestinal tumors through regulating IL-1β release and gut microbiota composition"
Article Title: Gasdermin D promotes development of intestinal tumors through regulating IL-1β release and gut microbiota composition
Journal: Cell Communication and Signaling : CCS
doi: 10.1186/s12964-024-01890-6
Figure Legend Snippet: GSDMD is upregulated in intestinal cancer. ( A ) IHC analysis of GSDMD protein level in a human tissue microarray, including 80 colorectal cancer and matched adjacent tumor specimens. ( B ) IHC analysis of GSDMD protein level in four colorectal cancer tissue and matched adjacent cancer tissue from ( A ) (200× magnification). ( C - E ) GSDMD expression score in cytoplasm ( C ), nuclear ( D ), and cytoplasm + nuclear ( E ) as calculated by multiplying the intensity and positive percentage scores according to ( A ). ( F ) Western blot analysis of GSDMD in in small intestine tumor tissue from Apc min/+ mice ( n = 5) and normal tissue from WT mice ( n = 3). Data are representative of at least three independent experiments (mean ± SEM). *** p < 0.001 by Student’s t test. FL means full length
Techniques Used: Microarray, Expressing, Western Blot
Figure Legend Snippet: Deficiency of GSDMD suppresses spontaneous intestinal cancer development. ( A ) Macroscopic view of representative small intestine from 20-week old Apc min/+ and Apc min/+ Gsdmd −/− mice. ( B ) Hematoxylin and eosin (H&E) staining of the representative intestinal tumor from the Apc min/+ and Apc min/+ Gsdmd −/− mice as in ( A ) (100× magnification). ( C - F ) Tumor number ( C and E ) and tumor load ( D and F ) from the small intestines or colons of 20-week-old Apc min/+ ( n = 7) and Apc min/+ Gsdmd −/− ( n = 7) mice. ( G ) Histogram showing the size distribution of tumors from the small intestines of 20-week-old Apc min/+ ( n = 7) and Apc min/+ Gsdmd −/− ( n = 7) mice. ( H - J ) Hematocrit ( H ), thymus weight ( I ) and spleen weight ( J ) of 20-week-old Apc min/+ ( n = 6–7) and Apc min/+ Gsdmd −/− ( n = 6–7) mice. Data are representative of at least three independent experiments (mean ± SEM). * p < 0.05, ** p < 0.01, *** p < 0.001 by Student’s t test
Techniques Used: Staining
Figure Legend Snippet: Exogenous IL-1β promotes intestinal tumor development in Apc min/+ Gsdmd −/− mice. ( A ) 8-week-old Apc min/+ Gsdmd −/− mice simultaneously received injection of IL-1β or PBS twice a week, while age and sex-matched Apc min/+ mice were injected with PBS ( n = 5–6/group). H&E staining of the representative intestinal tumor from the 20-week old above mice (100× magnification). Tumors are circled with dashed lines. ( B - E ) Tumor number ( B and D ) and tumor load ( C and E ) from the small intestines or colons of 20-week-old above mice as in ( A ). ( F - G ) Spleen weight ( F ), and hematocrit ( G ) of 20-week-old above mice as in ( A ). ( H ) Histogram showing the size distribution of tumors from the small intestines of 20-week-old above mice as in ( A ). Data are representative of at least three independent experiments (mean ± SEM). ** p < 0.01 by Student’s t test. N.S. means no significance
Techniques Used: Injection, Staining
Figure Legend Snippet: Exogenous Kyn promotes intestinal tumor development in Apc min/+ Gsdmd −/− mice. ( A ) ELISA analysis of Kyn and Trp from colon of Apc min/+ ( n = 6) and Apc min/+ Gsdmd −/− ( n = 6) mice. ( B ) Kyn/Trp was determined as in ( A ). ( C ) 8-week-old Apc min/+ Gsdmd −/− mice simultaneously received injection of Kyn or PBS once a week, while age and sex-matched Apc min/+ mice were injected with PBS ( n = 5/group). H&E staining of the representative intestinal tumor from the 20-week old above mice (100× magnification). ( D - G ) Tumor number ( D and F ) and tumor load ( E and G ) from the small intestines or colons of 20-week-old above mice as in ( C ). ( H - I ) Spleen weight ( H ), and hematocrit ( I ) of 20-week-old above mice as in ( C ). ( J ) Histogram showing the size distribution of tumors from the small intestines of 20-week-old above mice as in ( C ). Data are representative of at least three independent experiments (mean ± SEM). * p < 0.05, ** p < 0.01, *** p < 0.001 by Student’s t test. N.S. means no significance
Techniques Used: Enzyme-linked Immunosorbent Assay, Injection, Staining
Figure Legend Snippet: Exogenous Kyn increases Treg cell number in Apc min/+ Gsdmd −/− mice. ( A ) 8-week-old Apc min/+ Gsdmd −/− mice simultaneously received injection of Kyn or PBS once a week, while age and sex-matched Apc min/+ mice were injected with PBS ( n = 4/group). Flow cytometry analysis of CD4 + and CD8 + T cell number from intestinal tumor of the 20-week old above mice. ( B ) Total leukocytes were determined by CD45 + cell. CD4 + T cells % of total leukocyte and CD8 + T cells % of total leukocyte were determined as in ( A ). ( C ) Flow cytometry analysis of Treg (CD4 + Foxp3 + ) cell number from intestinal tumor of the 20-week old mice as in ( A ). ( D ) Treg % of CD4 + cells was determined as in ( C ). Data are representative of at least three independent experiments (mean ± SEM). ** p < 0.01, *** p < 0.001 by Student’s t test. N.S. means no significance
Techniques Used: Injection, Flow Cytometry
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