polyclonal antibody against sh3gl1 (Santa Cruz Biotechnology)
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Santa Cruz Biotechnology
polyclonal antibody against sh3gl1
Polyclonal Antibody Against Sh3gl1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 60 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/immunohistochemistry+microarray+based+expression+data/Ets+Family+Gel+Shift+Oligonucleotides/pmc03548755-117-3-8
Average 93 stars, based on 60 article reviews
Polyclonal Antibody Against Sh3gl1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 60 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/immunohistochemistry+microarray+based+expression+data/Ets+Family+Gel+Shift+Oligonucleotides/pmc03548755-117-3-8
Average 93 stars, based on 60 article reviews
polyclonal antibody against sh3gl1 - by Bioz Stars,
2026-10
93/100 stars
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1) Product Images from "Autologous antibody to src-homology 3-domain GRB2-like 1 specifically increases in the sera of patients with low-grade gliomas"
Article Title: Autologous antibody to src-homology 3-domain GRB2-like 1 specifically increases in the sera of patients with low-grade gliomas
Journal: Journal of Experimental & Clinical Cancer Research : CR
doi: 10.1186/1756-9966-31-85
Figure Legend Snippet: Genes identified by SEREX
Techniques Used: Sequencing, Recombinant, Amplification, Binding Assay, Coagulation, Variant Assay, Migration
Figure Legend Snippet: The increasing levels of antibodies to SH3GL1 in sera of the patients with low-grade glioma. Serum antibody level to SH3GL1 was examined by the ELISA as described in the legends of Figure . First screening test (A) and the individual validation test (B) , revealed the significant higher levels of autologous antibody against SH3GL1 in low-grade glioma patients, than healthy donors (P = 0.045 and 0.0189).
Techniques Used: Enzyme-linked Immunosorbent Assay, Biomarker Discovery
Figure Legend Snippet: Kaplan-Meier analysis for the overall survival of the patients with low-grade gliomas according to the serum level of anti-SH3GL1 autoantibody. The patients with higher serum level of anti-SH3GL1 autoantibody (solid line) survived significantly longer than those with lower levels (gray line) (p = 0.0124).
Techniques Used:
Figure Legend Snippet: Comparison of serum antibody levels among deletion mutants of SH3GL1. To confirm the epitope site, some SH3GL1 deletion mutants (A) were synthesized. Serum antibody levels were examined by ELISA with SH3GL1 muta-1 (B) , mut-2 (C) , mut-3 (D) and mut-4 (E) , and the 10–20 amino acids at the C-terminal end were indicated as the epitope site.
Techniques Used: Comparison, Synthesized, Enzyme-linked Immunosorbent Assay
Figure Legend Snippet: The detection of epitope site by overlapped peptide array. Series of peptides of 14 amino acid residues, composed of SH3GL1, were synthesized with overlapping by 12 amino acids, and were blotted in nitrocellulose membranes using F-moc amino acids (A) . Three sera of the patients with low-grade glioma indicated the fine reaction in spot 177 and 178 (C) , compared to two normal volunteers (D) and no serum control (B) . The calculated fluorescence intensity, normalized by background control, revealed that these spots were suggested as a minimum epitope site (E).
Techniques Used: Peptide Microarray, Synthesized, Control, Fluorescence
Figure Legend Snippet: Immunohistochemical analysis of SH3GL1 in glioma cells. Immunohistochemical stain for SH3GL1 in whole normal brain, consisted of white matter and gray matter (A) , and three representative results of normal white matter, low-grade glioma and high-grade glioma (B) were shown. Immunostaining for SH3GL1 was classified in five groups, and numbers of tissues in each group were scored (C).
Techniques Used: Immunohistochemical staining, Staining, Immunostaining
Figure Legend Snippet: Changes in the serum autoantibody level to SH3GL1 in a rat brain tumor model using C6 rat glioblastoma cells which were confirmed to express SH3GL1 protein. MRI studies show a steady growth of tumor mass in the rat brain (A) . The serum autoantibody levels were significantly increased at 2-week after tumor inoculation, and tended to decrease at 4-week after the inoculation (B).
Techniques Used:
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