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goat anti grp78 antibody  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology goat anti grp78 antibody
    <t> GRP78 </t> mRNA Expression Based on Microarray Analysis
    Goat Anti Grp78 Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1408 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/immunohistochemistry+microarray+based+expression+data/GRP+78+Antibody/pmc03234602-60-37-45
    Average 96 stars, based on 1408 article reviews
    goat anti grp78 antibody - by Bioz Stars, 2026-11
    96/100 stars

    Images

    1) Product Images from "Characteristic Upregulation of Glucose-Regulated Protein 78 in an Early Lesion Negative for Hitherto Established Cytochemical Markers in Rat Hepatocarcinogenesis"

    Article Title: Characteristic Upregulation of Glucose-Regulated Protein 78 in an Early Lesion Negative for Hitherto Established Cytochemical Markers in Rat Hepatocarcinogenesis

    Journal: Journal of Toxicologic Pathology

    doi: 10.1293/tox.22.281


    Figure Legend Snippet: GRP78 mRNA Expression Based on Microarray Analysis

    Techniques Used: Expressing, Microarray

    The relative expression of GRP78 mRNA in GST-P-negative HAF induced by DEN→clofibrate was slightly increased compared with the adjacent normal tissue. The vertical axis represents the ratio of GRP78 mRNA expression versus GAPDH mRNA expression in each region. The white bars represent GRP78 mRNA expression in the normal tissue, and the left hatched and black bars represent GST-P-positive and GST-P-negative HAF, respectively.
    Figure Legend Snippet: The relative expression of GRP78 mRNA in GST-P-negative HAF induced by DEN→clofibrate was slightly increased compared with the adjacent normal tissue. The vertical axis represents the ratio of GRP78 mRNA expression versus GAPDH mRNA expression in each region. The white bars represent GRP78 mRNA expression in the normal tissue, and the left hatched and black bars represent GST-P-positive and GST-P-negative HAF, respectively.

    Techniques Used: Expressing

    Immunohistochemistry for GRP78 and GST-P in HAF induced by DEN→clofibrate. (a) GST-P-negative lesion: 1, HE staining; 2, GRP78; 3, GST-P. (b) GST-P positive lesion: 1, HE staining; 2, GRP78; 3, GST-P. Lens × 4.
    Figure Legend Snippet: Immunohistochemistry for GRP78 and GST-P in HAF induced by DEN→clofibrate. (a) GST-P-negative lesion: 1, HE staining; 2, GRP78; 3, GST-P. (b) GST-P positive lesion: 1, HE staining; 2, GRP78; 3, GST-P. Lens × 4.

    Techniques Used: Immunohistochemistry, Staining

    Immunohistochemistry for GRP78 in GST-P-negative HCAs induced by DEN→clofibrate. (a) HE staining. (b) GST-P. (c), (d) GRP78. (a)-(c) lens × 4; (d) lens × 10.
    Figure Legend Snippet: Immunohistochemistry for GRP78 in GST-P-negative HCAs induced by DEN→clofibrate. (a) HE staining. (b) GST-P. (c), (d) GRP78. (a)-(c) lens × 4; (d) lens × 10.

    Techniques Used: Immunohistochemistry, Staining

    Related Articles

    Immunohistochemistry:

    Article Title: Isthmin 1 for treatment of lung inflammation
    Article Snippet: Wild-type mice (FVB/NTac, C57BL/6J, and BALB/cAnNTac; 6 to 8 weeks of age) were purchased from InVivos Pte Ltd, Singapore. .. Primary antibodies used for immunohistochemistry: anti-MMP-12 (ab66157, Abcam), anti-MMP-9 (ab38898, Abcam), anti-TGF-β31 (V, Santa Cruz Biotechnology), anti-VEGF-A (A-20, Santa Cruz Biotechnology), anti-ISM1 (for mouse lung: E-20, Santa Cruz Biotechnology; for human lung: custom antibody 3M8, AbMart), anti-GRP78 (A-10, Santa Cruz Biotechnology), anti-His-probe (H-15, Santa Cruz Biotechnology), anti-Cleaved caspase-3 (Asp175, Cell Signaling Technology). .. Primary antibodies used for immunofluorescence: anti-ISM1 (E-20, Santa Cruz Biotechnology), anti-p65 (10745-1-AP, Proteintech), anti-CD68 (M-20, Santa Cruz Biotechnology), anti-His-probe (H-15, Santa Cruz Biotechnology), anti-GRP78 (A-10, Santa Cruz Biotechnology), anti-Cleaved caspase-3 (Asp175, Cell Signaling Technology), anti-SP-C (FL-197, Santa Cruz Biotechnology), anti-PCNA (PC10, Santa Cruz Biotechnology), anti-GRP78 (A-10, Santa Cruz Biotechnology), Neutrophil Marker (NIMP-R14, Santa Cruz Biotechnology).

    Article Title: Investigation of molecular effects of beta caryophyllene in cadmium-induced nephrotoxicity model in rats: in silico and in vivo studies.
    Article Snippet: Background: Cadmium is one of the toxic heavy metals that accumulate in the kidneys, and it can cause kidney damage by triggering oxidative stress, ER stress, inflammation, and apoptosis.. β-caryophyllene (BCP) is a volatile ingredient found in various spice essential oils, known for its anti-inflammatory and strong oxygen-free radical scavenging properties.. In this study, we aimed to test the effects of BCP on kidney damage caused by Cd

    Saline:

    Article Title: Homoharringtonine exhibits senotherapeutic activity that mitigates diet- and age-associated obesity and insulin resistance and extends lifespan in mice
    Article Snippet: .. Both tissue sections and cell samples were permeabilized with 0.2% Triton X-100 in TBS (tris-buffered saline) for 10 min. Tissue sections were blocked with 5% normal donkey serum containing 1% bovine serum albumin (BSA), and cell samples were blocked with 5% BSA (MP Biomedicals, 160069) in PBS for 1 h. Tissue sections were then incubated with a mouse anti-HSPA5 antibody (Santa Cruz Biotechnology, sc-166490; 1:100) at 4 °C overnight. .. Cell samples were incubated with a mixture of a mouse anti-HSPA5 antibody (Santa Cruz Biotechnology, sc-166490; 1:100) and a rabbit anti–cleaved caspase-3 antibody (Cell Signaling Technology, #9664S; 1:100) at 4 °C overnight.

    Incubation:

    Article Title: Homoharringtonine exhibits senotherapeutic activity that mitigates diet- and age-associated obesity and insulin resistance and extends lifespan in mice
    Article Snippet: .. Both tissue sections and cell samples were permeabilized with 0.2% Triton X-100 in TBS (tris-buffered saline) for 10 min. Tissue sections were blocked with 5% normal donkey serum containing 1% bovine serum albumin (BSA), and cell samples were blocked with 5% BSA (MP Biomedicals, 160069) in PBS for 1 h. Tissue sections were then incubated with a mouse anti-HSPA5 antibody (Santa Cruz Biotechnology, sc-166490; 1:100) at 4 °C overnight. .. Cell samples were incubated with a mixture of a mouse anti-HSPA5 antibody (Santa Cruz Biotechnology, sc-166490; 1:100) and a rabbit anti–cleaved caspase-3 antibody (Cell Signaling Technology, #9664S; 1:100) at 4 °C overnight.

    Article Title: Isthmin 1 for treatment of lung inflammation
    Article Snippet: Peak fractions from the SEC were collected, concentrated with Amicon Ultra Centrifugal Filters (Millipore), and measured for protein concentration with Bradford assay. .. The purified proteins were finally aliquoted, snap-frozen with liquid nitrogen and stored in −80° C. Co-Immunoprecipitation To perform Co-IP assay between purified recombinant ISM1 and GRP78 proteins, 2 μg GRP78 antibody (LS-C165064, LSBio) was first conjugated to protein A/G agarose beads (Santa Cruz Biotechnology) via incubation with 20 μL beads in PBS at 4° C. for 2 hours. ..

    Article Title: Homoharringtonine exhibits senotherapeutic activity that mitigates diet- and age-associated obesity and insulin resistance and extends lifespan in mice
    Article Snippet: Both tissue sections and cell samples were permeabilized with 0.2% Triton X-100 in TBS (tris-buffered saline) for 10 min. Tissue sections were blocked with 5% normal donkey serum containing 1% bovine serum albumin (BSA), and cell samples were blocked with 5% BSA (MP Biomedicals, 160069) in PBS for 1 h. Tissue sections were then incubated with a mouse anti-HSPA5 antibody (Santa Cruz Biotechnology, sc-166490; 1:100) at 4 °C overnight. .. Cell samples were incubated with a mixture of a mouse anti-HSPA5 antibody (Santa Cruz Biotechnology, sc-166490; 1:100) and a rabbit anti–cleaved caspase-3 antibody (Cell Signaling Technology, #9664S; 1:100) at 4 °C overnight. ..

    Purification:

    Article Title: Isthmin 1 for treatment of lung inflammation
    Article Snippet: Peak fractions from the SEC were collected, concentrated with Amicon Ultra Centrifugal Filters (Millipore), and measured for protein concentration with Bradford assay. .. The purified proteins were finally aliquoted, snap-frozen with liquid nitrogen and stored in −80° C. Co-Immunoprecipitation To perform Co-IP assay between purified recombinant ISM1 and GRP78 proteins, 2 μg GRP78 antibody (LS-C165064, LSBio) was first conjugated to protein A/G agarose beads (Santa Cruz Biotechnology) via incubation with 20 μL beads in PBS at 4° C. for 2 hours. ..

    Co-Immunoprecipitation Assay:

    Article Title: Isthmin 1 for treatment of lung inflammation
    Article Snippet: Peak fractions from the SEC were collected, concentrated with Amicon Ultra Centrifugal Filters (Millipore), and measured for protein concentration with Bradford assay. .. The purified proteins were finally aliquoted, snap-frozen with liquid nitrogen and stored in −80° C. Co-Immunoprecipitation To perform Co-IP assay between purified recombinant ISM1 and GRP78 proteins, 2 μg GRP78 antibody (LS-C165064, LSBio) was first conjugated to protein A/G agarose beads (Santa Cruz Biotechnology) via incubation with 20 μL beads in PBS at 4° C. for 2 hours. ..

    Recombinant:

    Article Title: Isthmin 1 for treatment of lung inflammation
    Article Snippet: Peak fractions from the SEC were collected, concentrated with Amicon Ultra Centrifugal Filters (Millipore), and measured for protein concentration with Bradford assay. .. The purified proteins were finally aliquoted, snap-frozen with liquid nitrogen and stored in −80° C. Co-Immunoprecipitation To perform Co-IP assay between purified recombinant ISM1 and GRP78 proteins, 2 μg GRP78 antibody (LS-C165064, LSBio) was first conjugated to protein A/G agarose beads (Santa Cruz Biotechnology) via incubation with 20 μL beads in PBS at 4° C. for 2 hours. ..

    Immunofluorescence:

    Article Title: Isthmin 1 for treatment of lung inflammation
    Article Snippet: Primary antibodies used for immunohistochemistry: anti-MMP-12 (ab66157, Abcam), anti-MMP-9 (ab38898, Abcam), anti-TGF-β31 (V, Santa Cruz Biotechnology), anti-VEGF-A (A-20, Santa Cruz Biotechnology), anti-ISM1 (for mouse lung: E-20, Santa Cruz Biotechnology; for human lung: custom antibody 3M8, AbMart), anti-GRP78 (A-10, Santa Cruz Biotechnology), anti-His-probe (H-15, Santa Cruz Biotechnology), anti-Cleaved caspase-3 (Asp175, Cell Signaling Technology). .. Primary antibodies used for immunofluorescence: anti-ISM1 (E-20, Santa Cruz Biotechnology), anti-p65 (10745-1-AP, Proteintech), anti-CD68 (M-20, Santa Cruz Biotechnology), anti-His-probe (H-15, Santa Cruz Biotechnology), anti-GRP78 (A-10, Santa Cruz Biotechnology), anti-Cleaved caspase-3 (Asp175, Cell Signaling Technology), anti-SP-C (FL-197, Santa Cruz Biotechnology), anti-PCNA (PC10, Santa Cruz Biotechnology), anti-GRP78 (A-10, Santa Cruz Biotechnology), Neutrophil Marker (NIMP-R14, Santa Cruz Biotechnology). .. Reactive oxygen species was measured using OxiSelectTM In Vitro ROS/RNS Assay (STA-347, Cell Biolabs) according to manufacturer's protocol.

    Marker:

    Article Title: Isthmin 1 for treatment of lung inflammation
    Article Snippet: Primary antibodies used for immunohistochemistry: anti-MMP-12 (ab66157, Abcam), anti-MMP-9 (ab38898, Abcam), anti-TGF-β31 (V, Santa Cruz Biotechnology), anti-VEGF-A (A-20, Santa Cruz Biotechnology), anti-ISM1 (for mouse lung: E-20, Santa Cruz Biotechnology; for human lung: custom antibody 3M8, AbMart), anti-GRP78 (A-10, Santa Cruz Biotechnology), anti-His-probe (H-15, Santa Cruz Biotechnology), anti-Cleaved caspase-3 (Asp175, Cell Signaling Technology). .. Primary antibodies used for immunofluorescence: anti-ISM1 (E-20, Santa Cruz Biotechnology), anti-p65 (10745-1-AP, Proteintech), anti-CD68 (M-20, Santa Cruz Biotechnology), anti-His-probe (H-15, Santa Cruz Biotechnology), anti-GRP78 (A-10, Santa Cruz Biotechnology), anti-Cleaved caspase-3 (Asp175, Cell Signaling Technology), anti-SP-C (FL-197, Santa Cruz Biotechnology), anti-PCNA (PC10, Santa Cruz Biotechnology), anti-GRP78 (A-10, Santa Cruz Biotechnology), Neutrophil Marker (NIMP-R14, Santa Cruz Biotechnology). .. Reactive oxygen species was measured using OxiSelectTM In Vitro ROS/RNS Assay (STA-347, Cell Biolabs) according to manufacturer's protocol.

    Blocking Assay:

    Article Title: ELK1/NOL3/GRP78 axis regulates proliferation and stemness in TP53-mutant colon cancer by enhancing adaptive endoplasmic reticulum stress.
    Article Snippet: Background: Colon cancer harboring TP53 mutations is highly aggressive and associated with short survival.. Adaptive, rather than apoptotic, endoplasmic reticulum (ER) stress endows TP53-mutant tumor cells with enhanced protein-folding capacity, metabolic plasticity, and chemoresistance.. However, the upstream regulators that selectively drive this cytoprotective program within the cancer stem cell compartment remain elusive.



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