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Viramed Biotech immunoblot microarray
Immunoblot Microarray, supplied by Viramed Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/immunoblot+microarray/immunoblot/pmc07851003-33-2-4
Average 90 stars, based on 1 article reviews
immunoblot microarray - by Bioz Stars, 2026-11
90/100 stars

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Related Articles

Western Blot:

Article Title: Comparison of Western Immunobloting to an Enzyme-Linked Immunosorbent Assay for the Determination of Anti-Bordetella pertussis Antibodies
Article Snippet: .. In this study, we compared the Viramed immunoblot with the Virotech immunoblot using an ELISA as a reference method in the absence of patient histories or culture results; however, it has been established that a rise in anti-B. pertussis antibodies against PT and FHA is a clear indication of either active disease or vaccination (7, 10–12). .. The comparison between the Viramed immunoblot and the Virotech was complicated by the fact that the immunoblots differed with regard to the concentration of antigens used and types of antigens.

Article Title: Comparison of Western Immunobloting to an Enzyme-Linked Immunosorbent Assay for the Determination of Anti- Bordetella pertussis Antibodies
Article Snippet: .. In this study, we compared the Viramed immunoblot with the Virotech immunoblot using an ELISA as a reference method in the absence of patient histories or culture results; however, it has been established that a rise in anti- B. pertussis antibodies against PT and FHA is a clear indication of either active disease or vaccination ( 7 , 10 – 12 ). .. The comparison between the Viramed immunoblot and the Virotech was complicated by the fact that the immunoblots differed with regard to the concentration of antigens used and types of antigens.

Article Title: Comparison of post-Lyme Borreliosis symptoms in erythema migrans patients with positive and negative Borrelia burgdorferi sensu lato skin culture.
Article Snippet: Lyme Disease An Evidence-based Approach Edited by John J. Halperin, MD Atlantic Neurosciences Institute, New Jersey and Mount Sinai School of Medicine, New York Advances in Molecular and Cellular Microbiology 20 Advances in Molecular and Cellular Microbiology Through the application of molecular and cellular microbiology, we now recognise the diversity and dominance of microbial life forms on our planet that exist in all environments.. These microbes have many important planetary roles, but for we humans, a major problem is their ability to colonize our tissues and cause disease.. The same techniques of molecular and cellular microbiology have been applied to the problems of human and animal infection during the past two decades and have proved to be immensely powerful tools in elucidating how microorganisms cause human pathology.

Article Title: Diagnosis of COVID-19 using multiple antibody assays in two cases with negative PCR results from nasopharyngeal swabs
Article Snippet: .. Finally, an immunoblot microarray (Viramed, Munich, Germany), which concordantly detected high levels of IgM, IgA, and IgG against all viral proteins S1, S2, and nucleocapsid confirmed the presence of SARS-CoV-2-specific antibodies, and thereby SARS-CoV-2 infection. ..

Article Title: Comparison of Western Immunobloting to an Enzyme-Linked Immunosorbent Assay for the Determination of Anti- Bordetella pertussis Antibodies
Article Snippet: .. Of the 193 ELISA IgA negatives, 16 were positive by the Viramed immunoblot (see ) and 13 (6.7%) were equivocal. .. Agreement, sensitivity, and specificity, calculated by comparing the Viramed IgA immunoblot PT band to the Savyon IgA ELISA and excluding equivocal results, were 84.3%, 51.4% (95% CI, 38.5 to 63.0%), and 91.1% (95% CI, 88.5 to 93.5%), respectively ( ).

Article Title: Comparison of Western Immunobloting to an Enzyme-Linked Immunosorbent Assay for the Determination of Anti-Bordetella pertussis Antibodies
Article Snippet: .. In summary, the calibration of the Viramed and Virotech immunoblots with the WHO PT standard should make these serologic tests for B. pertussis antibodies more specific. .. The Viramed and Virotech assays are very similar to each other in sensitivity and specificity with regard to the detection of antibodies to PT compared to ELISA.

Enzyme-linked Immunosorbent Assay:

Article Title: Comparison of Western Immunobloting to an Enzyme-Linked Immunosorbent Assay for the Determination of Anti-Bordetella pertussis Antibodies
Article Snippet: .. In this study, we compared the Viramed immunoblot with the Virotech immunoblot using an ELISA as a reference method in the absence of patient histories or culture results; however, it has been established that a rise in anti-B. pertussis antibodies against PT and FHA is a clear indication of either active disease or vaccination (7, 10–12). .. The comparison between the Viramed immunoblot and the Virotech was complicated by the fact that the immunoblots differed with regard to the concentration of antigens used and types of antigens.

Article Title: Comparison of Western Immunobloting to an Enzyme-Linked Immunosorbent Assay for the Determination of Anti- Bordetella pertussis Antibodies
Article Snippet: .. In this study, we compared the Viramed immunoblot with the Virotech immunoblot using an ELISA as a reference method in the absence of patient histories or culture results; however, it has been established that a rise in anti- B. pertussis antibodies against PT and FHA is a clear indication of either active disease or vaccination ( 7 , 10 – 12 ). .. The comparison between the Viramed immunoblot and the Virotech was complicated by the fact that the immunoblots differed with regard to the concentration of antigens used and types of antigens.

Article Title: Comparison of Western Immunobloting to an Enzyme-Linked Immunosorbent Assay for the Determination of Anti- Bordetella pertussis Antibodies
Article Snippet: .. Of the 193 ELISA IgA negatives, 16 were positive by the Viramed immunoblot (see ) and 13 (6.7%) were equivocal. .. Agreement, sensitivity, and specificity, calculated by comparing the Viramed IgA immunoblot PT band to the Savyon IgA ELISA and excluding equivocal results, were 84.3%, 51.4% (95% CI, 38.5 to 63.0%), and 91.1% (95% CI, 88.5 to 93.5%), respectively ( ).

other:


Article Title: Abstract of 29th Regional Congress of the ISBT
Article Snippet: Reactive results were confirmed with immunoblots (Viramed Biotech AG, Planegg, Germany), which discriminated both specific anti‐Tp (IgG, IgM) and non‐specific VDRL antibodies in five dilutions.

Microarray:

Article Title: Diagnosis of COVID-19 using multiple antibody assays in two cases with negative PCR results from nasopharyngeal swabs
Article Snippet: .. Finally, an immunoblot microarray (Viramed, Munich, Germany), which concordantly detected high levels of IgM, IgA, and IgG against all viral proteins S1, S2, and nucleocapsid confirmed the presence of SARS-CoV-2-specific antibodies, and thereby SARS-CoV-2 infection. ..

Infection:

Article Title: Diagnosis of COVID-19 using multiple antibody assays in two cases with negative PCR results from nasopharyngeal swabs
Article Snippet: .. Finally, an immunoblot microarray (Viramed, Munich, Germany), which concordantly detected high levels of IgM, IgA, and IgG against all viral proteins S1, S2, and nucleocapsid confirmed the presence of SARS-CoV-2-specific antibodies, and thereby SARS-CoV-2 infection. ..



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(A and B) Schematic representation and arrangement of control and B. burgdorferi antigens for the ViraChip IgM and IgG <t>microarray</t> <t>immunoblot</t> (MIB) assays. (C and D) Representative images of positive and negative results, as indicated. (Images adapted and used with permission from Viramed Biotech AG.)
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(A and B) Schematic representation and arrangement of control and B. burgdorferi antigens for the ViraChip IgM and IgG <t>microarray</t> immunoblot (MIB) assays. (C and D) Representative images of positive and negative results, as indicated. (Images adapted and used with permission from Viramed Biotech AG.)
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(A and B) Schematic representation and arrangement of control and B. burgdorferi antigens for the ViraChip IgM and IgG <t>microarray</t> immunoblot (MIB) assays. (C and D) Representative images of positive and negative results, as indicated. (Images adapted and used with permission from Viramed Biotech AG.)
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(A and B) Schematic representation and arrangement of control and B. burgdorferi antigens for the <t>ViraChip</t> IgM and IgG microarray immunoblot (MIB) assays. (C and D) Representative images of positive and negative results, as indicated. (Images adapted and used with permission from Viramed Biotech AG.)
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Image Search Results


(A and B) Schematic representation and arrangement of control and B. burgdorferi antigens for the ViraChip IgM and IgG microarray immunoblot (MIB) assays. (C and D) Representative images of positive and negative results, as indicated. (Images adapted and used with permission from Viramed Biotech AG.)

Journal: Journal of Clinical Microbiology

Article Title: Evaluation of a Novel Microarray Immunoblot Assay for Detection of IgM- and IgG-Class Antibodies to Borrelia burgdorferi

doi: 10.1128/JCM.00992-18

Figure Lengend Snippet: (A and B) Schematic representation and arrangement of control and B. burgdorferi antigens for the ViraChip IgM and IgG microarray immunoblot (MIB) assays. (C and D) Representative images of positive and negative results, as indicated. (Images adapted and used with permission from Viramed Biotech AG.)

Article Snippet: In this study, we present the first evaluation of the recently FDA-cleared Borrelia B31 ViraChip IgM and IgG microarray immunoblot assays (ViraChip MIB assays; Viramed Biotech AG, Planegg, Germany), which were entirely automated on a Gemini Compact Microplate Processor (Stratec Biomedical AG, Birkenfeld, Germany).

Techniques: Control, Microarray, Western Blot

(A and B) Schematic representation and arrangement of control and B. burgdorferi antigens for the ViraChip IgM and IgG microarray immunoblot (MIB) assays. (C and D) Representative images of positive and negative results, as indicated. (Images adapted and used with permission from Viramed Biotech AG.)

Journal: Journal of Clinical Microbiology

Article Title: Evaluation of a Novel Microarray Immunoblot Assay for Detection of IgM- and IgG-Class Antibodies to Borrelia burgdorferi

doi: 10.1128/JCM.00992-18

Figure Lengend Snippet: (A and B) Schematic representation and arrangement of control and B. burgdorferi antigens for the ViraChip IgM and IgG microarray immunoblot (MIB) assays. (C and D) Representative images of positive and negative results, as indicated. (Images adapted and used with permission from Viramed Biotech AG.)

Article Snippet: All included samples were tested within one freeze-thaw cycle by the following second-tier supplemental assays: the Borrelia B31 ViraChip IgM and IgG microarray immunoblots (ViraChip MIB assays; Viramed Biotech AG, Planegg, Germany), the Borrelia B31 ViraStripe IgM and IgG LIAs (Viramed Biotech AG, Planegg, Germany), and the MarDx B. burgdorferi IgM and IgG WB assays (Trinity Biotech, Carlsbad, CA).

Techniques: Control, Microarray, Western Blot

(A and B) Schematic representation and arrangement of control and B. burgdorferi antigens for the ViraChip IgM and IgG microarray immunoblot (MIB) assays. (C and D) Representative images of positive and negative results, as indicated. (Images adapted and used with permission from Viramed Biotech AG.)

Journal: Journal of Clinical Microbiology

Article Title: Evaluation of a Novel Microarray Immunoblot Assay for Detection of IgM- and IgG-Class Antibodies to Borrelia burgdorferi

doi: 10.1128/JCM.00992-18

Figure Lengend Snippet: (A and B) Schematic representation and arrangement of control and B. burgdorferi antigens for the ViraChip IgM and IgG microarray immunoblot (MIB) assays. (C and D) Representative images of positive and negative results, as indicated. (Images adapted and used with permission from Viramed Biotech AG.)

Article Snippet: The substrate was subsequently removed, and wells were washed three times and allowed to dry under continuous airflow for 20 min prior to colorimetric imaging using a high-sensitivity charge-coupled-device (CCD) camera on a CLAIR reader (Sensovation, Stockach, Germany). fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window FIG 1 caption a7 (A and B) Schematic representation and arrangement of control and B. burgdorferi antigens for the ViraChip IgM and IgG microarray immunoblot (MIB) assays. (C and D) Representative images of positive and negative results, as indicated. (Images adapted and used with permission from Viramed Biotech AG.)

Techniques: Control, Microarray, Western Blot

Comparison of the  ViraChip  MIB assays and ViraStripe LIA for detection of anti- B. burgdorferi IgM and IgG antibodies in serum

Journal: Journal of Clinical Microbiology

Article Title: Evaluation of a Novel Microarray Immunoblot Assay for Detection of IgM- and IgG-Class Antibodies to Borrelia burgdorferi

doi: 10.1128/JCM.00992-18

Figure Lengend Snippet: Comparison of the ViraChip MIB assays and ViraStripe LIA for detection of anti- B. burgdorferi IgM and IgG antibodies in serum

Article Snippet: The substrate was subsequently removed, and wells were washed three times and allowed to dry under continuous airflow for 20 min prior to colorimetric imaging using a high-sensitivity charge-coupled-device (CCD) camera on a CLAIR reader (Sensovation, Stockach, Germany). fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window FIG 1 caption a7 (A and B) Schematic representation and arrangement of control and B. burgdorferi antigens for the ViraChip IgM and IgG microarray immunoblot (MIB) assays. (C and D) Representative images of positive and negative results, as indicated. (Images adapted and used with permission from Viramed Biotech AG.)

Techniques: Comparison

Comparison of the  ViraChip  MIB assay and MarDx WB assay for detection of anti- B. burgdorferi IgM and IgG antibodies in serum

Journal: Journal of Clinical Microbiology

Article Title: Evaluation of a Novel Microarray Immunoblot Assay for Detection of IgM- and IgG-Class Antibodies to Borrelia burgdorferi

doi: 10.1128/JCM.00992-18

Figure Lengend Snippet: Comparison of the ViraChip MIB assay and MarDx WB assay for detection of anti- B. burgdorferi IgM and IgG antibodies in serum

Article Snippet: The substrate was subsequently removed, and wells were washed three times and allowed to dry under continuous airflow for 20 min prior to colorimetric imaging using a high-sensitivity charge-coupled-device (CCD) camera on a CLAIR reader (Sensovation, Stockach, Germany). fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window FIG 1 caption a7 (A and B) Schematic representation and arrangement of control and B. burgdorferi antigens for the ViraChip IgM and IgG microarray immunoblot (MIB) assays. (C and D) Representative images of positive and negative results, as indicated. (Images adapted and used with permission from Viramed Biotech AG.)

Techniques: Comparison, Western Blot

Comparison of the  ViraChip IgM  and IgG MIB assays to a consensus standard for the presence or absence of IgM and IgG antibodies to B. burgdorferi

Journal: Journal of Clinical Microbiology

Article Title: Evaluation of a Novel Microarray Immunoblot Assay for Detection of IgM- and IgG-Class Antibodies to Borrelia burgdorferi

doi: 10.1128/JCM.00992-18

Figure Lengend Snippet: Comparison of the ViraChip IgM and IgG MIB assays to a consensus standard for the presence or absence of IgM and IgG antibodies to B. burgdorferi

Article Snippet: The substrate was subsequently removed, and wells were washed three times and allowed to dry under continuous airflow for 20 min prior to colorimetric imaging using a high-sensitivity charge-coupled-device (CCD) camera on a CLAIR reader (Sensovation, Stockach, Germany). fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window FIG 1 caption a7 (A and B) Schematic representation and arrangement of control and B. burgdorferi antigens for the ViraChip IgM and IgG microarray immunoblot (MIB) assays. (C and D) Representative images of positive and negative results, as indicated. (Images adapted and used with permission from Viramed Biotech AG.)

Techniques: Comparison

Performance of the ViraStripe LIA and  ViraChip  MIB assay in clinically characterized sera

Journal: Journal of Clinical Microbiology

Article Title: Evaluation of a Novel Microarray Immunoblot Assay for Detection of IgM- and IgG-Class Antibodies to Borrelia burgdorferi

doi: 10.1128/JCM.00992-18

Figure Lengend Snippet: Performance of the ViraStripe LIA and ViraChip MIB assay in clinically characterized sera

Article Snippet: The substrate was subsequently removed, and wells were washed three times and allowed to dry under continuous airflow for 20 min prior to colorimetric imaging using a high-sensitivity charge-coupled-device (CCD) camera on a CLAIR reader (Sensovation, Stockach, Germany). fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window FIG 1 caption a7 (A and B) Schematic representation and arrangement of control and B. burgdorferi antigens for the ViraChip IgM and IgG microarray immunoblot (MIB) assays. (C and D) Representative images of positive and negative results, as indicated. (Images adapted and used with permission from Viramed Biotech AG.)

Techniques: Control

Total processing times for the  ViraChip  MIB and ViraStripe LIA for a full batch of patient samples and controls per assay

Journal: Journal of Clinical Microbiology

Article Title: Evaluation of a Novel Microarray Immunoblot Assay for Detection of IgM- and IgG-Class Antibodies to Borrelia burgdorferi

doi: 10.1128/JCM.00992-18

Figure Lengend Snippet: Total processing times for the ViraChip MIB and ViraStripe LIA for a full batch of patient samples and controls per assay

Article Snippet: The substrate was subsequently removed, and wells were washed three times and allowed to dry under continuous airflow for 20 min prior to colorimetric imaging using a high-sensitivity charge-coupled-device (CCD) camera on a CLAIR reader (Sensovation, Stockach, Germany). fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window FIG 1 caption a7 (A and B) Schematic representation and arrangement of control and B. burgdorferi antigens for the ViraChip IgM and IgG microarray immunoblot (MIB) assays. (C and D) Representative images of positive and negative results, as indicated. (Images adapted and used with permission from Viramed Biotech AG.)

Techniques:

(A and B) Schematic representation and arrangement of control and B. burgdorferi antigens for the ViraChip IgM and IgG microarray immunoblot (MIB) assays. (C and D) Representative images of positive and negative results, as indicated. (Images adapted and used with permission from Viramed Biotech AG.)

Journal: Journal of Clinical Microbiology

Article Title: Evaluation of a Novel Microarray Immunoblot Assay for Detection of IgM- and IgG-Class Antibodies to Borrelia burgdorferi

doi: 10.1128/JCM.00992-18

Figure Lengend Snippet: (A and B) Schematic representation and arrangement of control and B. burgdorferi antigens for the ViraChip IgM and IgG microarray immunoblot (MIB) assays. (C and D) Representative images of positive and negative results, as indicated. (Images adapted and used with permission from Viramed Biotech AG.)

Article Snippet: We present the first analytic evaluation of the automated Borrelia B31 ViraChip IgM and IgG microarray immunoblot (MIB) assays (Viramed Biotech AG, Planegg, Germany) in comparison to two different, semiautomated blot assays for LD, including the Borrelia B31 ViraStripe IgM and IgG line immunoassays (LIAs) (Viramed) and the MarDx Borrelia burgdorferi IgM and IgG Western blot (WB) assays (Trinity Biotech, Carlsbad, CA), using prospectively collected sera ( n = 411) and archived, clinically characterized samples ( n = 91).

Techniques: Microarray, Western Blot

Comparison of the  ViraChip  MIB assays and ViraStripe LIA for detection of anti- B. burgdorferi IgM and IgG antibodies in serum

Journal: Journal of Clinical Microbiology

Article Title: Evaluation of a Novel Microarray Immunoblot Assay for Detection of IgM- and IgG-Class Antibodies to Borrelia burgdorferi

doi: 10.1128/JCM.00992-18

Figure Lengend Snippet: Comparison of the ViraChip MIB assays and ViraStripe LIA for detection of anti- B. burgdorferi IgM and IgG antibodies in serum

Article Snippet: We present the first analytic evaluation of the automated Borrelia B31 ViraChip IgM and IgG microarray immunoblot (MIB) assays (Viramed Biotech AG, Planegg, Germany) in comparison to two different, semiautomated blot assays for LD, including the Borrelia B31 ViraStripe IgM and IgG line immunoassays (LIAs) (Viramed) and the MarDx Borrelia burgdorferi IgM and IgG Western blot (WB) assays (Trinity Biotech, Carlsbad, CA), using prospectively collected sera ( n = 411) and archived, clinically characterized samples ( n = 91).

Techniques:

Comparison of the  ViraChip  MIB assay and MarDx WB assay for detection of anti- B. burgdorferi IgM and IgG antibodies in serum

Journal: Journal of Clinical Microbiology

Article Title: Evaluation of a Novel Microarray Immunoblot Assay for Detection of IgM- and IgG-Class Antibodies to Borrelia burgdorferi

doi: 10.1128/JCM.00992-18

Figure Lengend Snippet: Comparison of the ViraChip MIB assay and MarDx WB assay for detection of anti- B. burgdorferi IgM and IgG antibodies in serum

Article Snippet: We present the first analytic evaluation of the automated Borrelia B31 ViraChip IgM and IgG microarray immunoblot (MIB) assays (Viramed Biotech AG, Planegg, Germany) in comparison to two different, semiautomated blot assays for LD, including the Borrelia B31 ViraStripe IgM and IgG line immunoassays (LIAs) (Viramed) and the MarDx Borrelia burgdorferi IgM and IgG Western blot (WB) assays (Trinity Biotech, Carlsbad, CA), using prospectively collected sera ( n = 411) and archived, clinically characterized samples ( n = 91).

Techniques: Western Blot

Comparison of the  ViraChip   IgM  and IgG MIB assays to a consensus standard for the presence or absence of  IgM  and IgG antibodies to B. burgdorferi

Journal: Journal of Clinical Microbiology

Article Title: Evaluation of a Novel Microarray Immunoblot Assay for Detection of IgM- and IgG-Class Antibodies to Borrelia burgdorferi

doi: 10.1128/JCM.00992-18

Figure Lengend Snippet: Comparison of the ViraChip IgM and IgG MIB assays to a consensus standard for the presence or absence of IgM and IgG antibodies to B. burgdorferi

Article Snippet: We present the first analytic evaluation of the automated Borrelia B31 ViraChip IgM and IgG microarray immunoblot (MIB) assays (Viramed Biotech AG, Planegg, Germany) in comparison to two different, semiautomated blot assays for LD, including the Borrelia B31 ViraStripe IgM and IgG line immunoassays (LIAs) (Viramed) and the MarDx Borrelia burgdorferi IgM and IgG Western blot (WB) assays (Trinity Biotech, Carlsbad, CA), using prospectively collected sera ( n = 411) and archived, clinically characterized samples ( n = 91).

Techniques:

Performance of the ViraStripe LIA and  ViraChip  MIB assay in clinically characterized sera

Journal: Journal of Clinical Microbiology

Article Title: Evaluation of a Novel Microarray Immunoblot Assay for Detection of IgM- and IgG-Class Antibodies to Borrelia burgdorferi

doi: 10.1128/JCM.00992-18

Figure Lengend Snippet: Performance of the ViraStripe LIA and ViraChip MIB assay in clinically characterized sera

Article Snippet: We present the first analytic evaluation of the automated Borrelia B31 ViraChip IgM and IgG microarray immunoblot (MIB) assays (Viramed Biotech AG, Planegg, Germany) in comparison to two different, semiautomated blot assays for LD, including the Borrelia B31 ViraStripe IgM and IgG line immunoassays (LIAs) (Viramed) and the MarDx Borrelia burgdorferi IgM and IgG Western blot (WB) assays (Trinity Biotech, Carlsbad, CA), using prospectively collected sera ( n = 411) and archived, clinically characterized samples ( n = 91).

Techniques:

Total processing times for the  ViraChip  MIB and ViraStripe LIA for a full batch of patient samples and controls per assay

Journal: Journal of Clinical Microbiology

Article Title: Evaluation of a Novel Microarray Immunoblot Assay for Detection of IgM- and IgG-Class Antibodies to Borrelia burgdorferi

doi: 10.1128/JCM.00992-18

Figure Lengend Snippet: Total processing times for the ViraChip MIB and ViraStripe LIA for a full batch of patient samples and controls per assay

Article Snippet: We present the first analytic evaluation of the automated Borrelia B31 ViraChip IgM and IgG microarray immunoblot (MIB) assays (Viramed Biotech AG, Planegg, Germany) in comparison to two different, semiautomated blot assays for LD, including the Borrelia B31 ViraStripe IgM and IgG line immunoassays (LIAs) (Viramed) and the MarDx Borrelia burgdorferi IgM and IgG Western blot (WB) assays (Trinity Biotech, Carlsbad, CA), using prospectively collected sera ( n = 411) and archived, clinically characterized samples ( n = 91).

Techniques: