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imagequant  (Cytiva Europe)


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    Cytiva Europe imagequant
    Imagequant, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 97/100, based on 6553 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/imagequant+radioactivity+analysis+program/ImageQuant+LAS+4000/pmc08096218-128-17-20
    Average 97 stars, based on 6553 article reviews
    imagequant - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    other:

    Article Title: A monocarboxylate transporter rescues frontotemporal dementia and Alzheimer’s disease models
    Article Snippet: Bands were visualized with Luminata Crescendo (Millipore) or Pierce ECL (Thermo Scientific) and imaged with ImageQuant LAS4000 (GE Healthcare Life Sciences).

    Article Title: Functional loss of rffG and rfbB, encoding dTDP-glucose 4,6-dehydratase, changes colony morphology, cell shape, motility and virulence in Salmonella Typhimurium
    Article Snippet: The plates were imaged using the ImageQuant LAS4000 (GE Healthcare).

    Article Title: Genome-wide screening in pluripotent cells identifies Mtf1 as a suppressor of mutant huntingtin toxicity
    Article Snippet: Pico SuperSignal West chemiluminescent reagent (Thermo Scientific, cat. 34078) was used to incubate membranes and images were digitally acquired by ImageQuant LAS 4000 (GE Healthcare).

    Article Title: Mediator kinase inhibition suppresses hyperactive interferon signaling in Down syndrome
    Article Snippet: Membranes were processed according to the manufacturer’s instructions and imaged using an ImageQuant LAS 4000 (GE Healthcare).

    Incubation:

    Article Title: Tyrosine Kinase Inhibitors Diminish Renal Neoplasms in a Tuberous Sclerosis Model Via Induction of Apoptosis
    Article Snippet: .. All membranes were washed with TBS-T, incubated with peroxidase (POD)-labeled secondary antibodies at room temperature, and visualized with Pierce ECL reagent (No. 32106, Thermo Fisher Scientific) and ImageQuant LAS 4000 Series imager (GE Healthcare Life Sciences). ..

    Article Title: Adipocytes promote pancreatic cancer migration and invasion through fatty acid metabolic reprogramming
    Article Snippet: A total of 20 μg protein was loaded per lane onto 12% SDS-gels, resolved using SDS-PAGE, transferred to 0.22 μm PVDF membranes (MilliporeSigma), and blocked for 1 h at room temperature with 5% skim milk. .. Each membrane was immunoblotted with the indicated primary antibodies at 4°C overnight and then incubated with a secondary antibody at 37°C for 1 h. Immunoreactive bands were visualized using an enhanced chemiluminescence detection kit (Thermo Fisher Scientific, Inc.) using an ImageQuant LAS 4000 System (GE Healthcare). .. The following primary and secondary antibodies were used in the present study: AKT (1:1,000; ProteinTech Group, Inc.; cat. no. 10176-2-AP), p-AKT Ser473 (1:1,000, Cell Signaling Technology, Inc.; cat. no. CST4060S), HIF-1α (1:1,000; ProteinTech Group, Inc.; cat. no. 20960-1-AP), ANGPTL4 (1:1,000; ProteinTech Group, Inc.; cat. no. 18374-1-AP), GLUT1 (1:1,000; ProteinTech Group, Inc.; cat. no. 66290-1-lg), HK2 (1:1,000; ProteinTech Group, Inc.; cat. no. 22029-1-AP), LDHA (1:1,000; ProteinTech Group, Inc.; cat. no. 19987-1-AP), α-tubulin (1:1,000; ProteinTech Group, Inc.; cat. no. 66031-1-lg), p-AMPKα Thr172 (1:1,000; Cell Signaling Technology, Inc.; cat. no. CST2535), AMPKα (1:1,000; ProteinTech Group, Inc.; cat. no. 10929-2-AP), p-STAT3 Tyr705 (1:1,000; Cell Signaling Technology, Inc.; cat. no. CST9145), STAT3 (1:1,000; Cell Signaling Technology, Inc.; CST12640), Snail (1:1,000; CST; CST9782), horseradish peroxidase-conjugated anti-rabbit IgG antibody (1:5,000; ProteinTech Group, Inc.; cat. no. SA00001-2) and goat anti-mouse IgG secondary antibody HRP conjugated (1:5,000; Signalway Antibody LLC; cat. no. L3032).

    Western Blot:

    Article Title: A leaf-emanated signal orchestrates grain size and number in response to maternal resources.
    Article Snippet: .. Signals were developed and detected using ECL Prime Western Blotting Detection Reagent and ImageQuant LAS 4000 (both GE Healthcare Life Sciences). ..

    Article Title: Selective Inhibition of ATM-dependent Double-strand Break Repair and Checkpoint Control Synergistically Enhances the Efficacy of ATR Inhibitors
    Article Snippet: The primary antibodies used were: p-ATM S1981 (Abcam #ab81292), ATM (Santa Cruz Biotechnology #SC23921), p-KAP1 S824 (Abcam #ab133440), KAP1 (Abcam #ab22553), p-CHK2 T68 (Cell Signaling Technology #2197), CHK2 (Cell Signaling Technology #6334), p-CHK1 S317 (Cell Signaling Technology #12302), CHK1 (Cell Signaling Technology #2360), p-p53 S15 (Cell Signaling Technology #9284), p53 (Cell Signaling Technology #48818), MDM2 (Cell Signaling Technology #86934), p21 (Cell Signaling Technology #2947), and horseradish peroxidase–conjugated GAPDH (Cell Signaling Technology #3683). .. Western blots were analyzed using GE ImageQuant LAS 4000 with SuperSignal West Pico Chemiluminescent Substrate (Thermo Fisher Scientific #34080) and/or SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher Scientific #34094). .. Cell-cycle analysis was performed using the FITC bromodeoxyuridine (BrdU) Flow Kit from BD Biosciences (#559619) according to the manufacturer's protocol.

    Membrane:

    Article Title: Adipocytes promote pancreatic cancer migration and invasion through fatty acid metabolic reprogramming
    Article Snippet: A total of 20 μg protein was loaded per lane onto 12% SDS-gels, resolved using SDS-PAGE, transferred to 0.22 μm PVDF membranes (MilliporeSigma), and blocked for 1 h at room temperature with 5% skim milk. .. Each membrane was immunoblotted with the indicated primary antibodies at 4°C overnight and then incubated with a secondary antibody at 37°C for 1 h. Immunoreactive bands were visualized using an enhanced chemiluminescence detection kit (Thermo Fisher Scientific, Inc.) using an ImageQuant LAS 4000 System (GE Healthcare). .. The following primary and secondary antibodies were used in the present study: AKT (1:1,000; ProteinTech Group, Inc.; cat. no. 10176-2-AP), p-AKT Ser473 (1:1,000, Cell Signaling Technology, Inc.; cat. no. CST4060S), HIF-1α (1:1,000; ProteinTech Group, Inc.; cat. no. 20960-1-AP), ANGPTL4 (1:1,000; ProteinTech Group, Inc.; cat. no. 18374-1-AP), GLUT1 (1:1,000; ProteinTech Group, Inc.; cat. no. 66290-1-lg), HK2 (1:1,000; ProteinTech Group, Inc.; cat. no. 22029-1-AP), LDHA (1:1,000; ProteinTech Group, Inc.; cat. no. 19987-1-AP), α-tubulin (1:1,000; ProteinTech Group, Inc.; cat. no. 66031-1-lg), p-AMPKα Thr172 (1:1,000; Cell Signaling Technology, Inc.; cat. no. CST2535), AMPKα (1:1,000; ProteinTech Group, Inc.; cat. no. 10929-2-AP), p-STAT3 Tyr705 (1:1,000; Cell Signaling Technology, Inc.; cat. no. CST9145), STAT3 (1:1,000; Cell Signaling Technology, Inc.; CST12640), Snail (1:1,000; CST; CST9782), horseradish peroxidase-conjugated anti-rabbit IgG antibody (1:5,000; ProteinTech Group, Inc.; cat. no. SA00001-2) and goat anti-mouse IgG secondary antibody HRP conjugated (1:5,000; Signalway Antibody LLC; cat. no. L3032).



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