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Charles River Laboratories
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Organisms, supplied by Charles River Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/image-processing+code+scanip/organisms+strains/pm36493773-272-34-37
Average 86 stars, based on 1 article reviews
Organisms, supplied by Charles River Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/image-processing+code+scanip/organisms+strains/pm36493773-272-34-37
Average 86 stars, based on 1 article reviews
organisms - by Bioz Stars,
2026-09
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Recombinant:Article Title: Protocol for a murine transient middle cerebral artery occlusion model with local intra-arterial drug delivery. Article Snippet: .. KEY RESOURCES TABLE REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Article Title: SEPHS2 loss reprograms cancer metabolism from oxidative phosphorylation to gluconeogenesis via PCK1 stabilization. Article Snippet: Upon arrival at the animal core facility of Southern University of Science and Technology, mice were acclimated for 1 week in a barrier facility to minimize the potential effects of .. REAGENT or RESOURCE SOURCE IDENTIFIER M&R HRP/DAB Detection IHC Kit Vazyme Cat#HC301-01; Lot#7E2762K4 Deposited data Transcriptomics: A-375 control and SEPHS2 knockout cells This paper GSE:324223 Proteomics:A-375 control and SEPHS2 knockout cells This paper PXD076137 Experimental models: Cell lines A-375 ATCC Cat#CRL-1619 HeLa ATCC Cat#CCL-2 A2058 CellCook Cat#CC1805 HEK-293FT CellCook Cat#CC4046 MCF-7 CellCook Cat#CC0302 Experimental models: Article Title: Distinct supraspinal neural pathways underlie licking-induced alleviation of pain sensation and aversion in male mice. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit Anti-Glutamate antibody Sigma-Aldrich Cat# G6642; RRID: AB_259946 Rabbit Anti-GABA antibody Sigma-Aldrich Cat# A2052; RRID: AB_477652 Rabbit Anti-TH antibody HUABIO Cat# ET1611-12; RRID: AB_3069989 Pig Anti-c-Fos antibody SYSY Cat# 226308; RRID: AB_2905595 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) Abcam Cat# ab150077; RRID: AB_2630356 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555) Abcam Cat# ab150078; RRID: AB_2722519 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) Abcam Cat# ab150079; RRID: AB_2722623 Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 488) Abcam Cat# ab150185; RRID: AB_2736871 Bacterial and virus strains rAAV-Ef1α-DIO-N2cG Brain Case Cat# BC-0304 rAAV-Ef1α-DIO-mCherry-F2A-TVA Brain Case Cat# BC-0061 CVS-EnvA-ΔG-EGFP Brain Case Cat# BC-RV-CVS EnVA461 rAAV2/1-hSyn-Cre-WPRE-hGH-pA BrainVTA Cat# PT-0136 rAAV-Ef1α-DIO-mCherry-WPRE-hGH BrainVTA Cat# PT-0795 rAAV-Ef1α-DIO-hChR2 (H134R)-mCherry-WPRE-pA BrainVTA Cat# PT-0002 rAAV-Ef1α-DIO-hM4D(Gi)-EGFP-WPRE-pA BrainVTA Cat# PT-0043 rAAV-Ef1α-DIO-hM3D(Gq)-mCherry-WPRE-pA BrainVTA Cat# PT-0042 retro-rAAV-Ef1α-DIO-hM4D(Gi)-EGFP-WPRE-pA BrainVTA Cat# PT-0987 retro-rAAV-Ef1α-DIO-hM3D(Gq)-mCherry-WPRE-pA BrainVTA Cat# PT-0042 retro-rAAV-hSyn-CRE-WPRE-hGH BrainVTA Cat# PT-0136 retro-rAAV-Ef1α-DIO-EGFP-WPRE-hGH BrainVTA Cat# PT-0795 retro-rAAV-Ef1α-DIO-mCherry-WPRE-hGH BrainVTA Cat# PT-0013 rAAV-Ef1α-DIO-GCaMP6s-WPRE-pA BrainVTA Cat# PT-0071 retro-rAAV-Ef1α-DIO-GCaMP6s-WPRE-pA BrainVTA Cat# PT-0071 rAAV-CAG-DIO-WGA-FLP-WPRE-hGH-pA BrainVTA Cat# PT-0557 rAAV-EF1a-fDIO-mCherry-WPRE-hGH-pA BrainVTA Cat# PT-1046 rAAV-cfos-tTA-WPRE-hGH-pA BrainVTA Cat# PT-3022 rAAV-TRE-tight-EGFP-WPREs BrainVTA Cat# PT-9667 Chemicals, peptides, and recombinant proteins TTX Tauto Biotech Cat# E− 2968 4-AP MCE HY-B0604 CNQX Tocris Cat# 0190 Bicuculine Selleck Cat# S7071 Clozapine N-oxide TargetMol Cat# T4494 Experimental models: Microscopy:Article Title: Protocol for a murine transient middle cerebral artery occlusion model with local intra-arterial drug delivery. Article Snippet: .. KEY RESOURCES TABLE REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Article Title: Distinct supraspinal neural pathways underlie licking-induced alleviation of pain sensation and aversion in male mice. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit Anti-Glutamate antibody Sigma-Aldrich Cat# G6642; RRID: AB_259946 Rabbit Anti-GABA antibody Sigma-Aldrich Cat# A2052; RRID: AB_477652 Rabbit Anti-TH antibody HUABIO Cat# ET1611-12; RRID: AB_3069989 Pig Anti-c-Fos antibody SYSY Cat# 226308; RRID: AB_2905595 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) Abcam Cat# ab150077; RRID: AB_2630356 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555) Abcam Cat# ab150078; RRID: AB_2722519 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) Abcam Cat# ab150079; RRID: AB_2722623 Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 488) Abcam Cat# ab150185; RRID: AB_2736871 Bacterial and virus strains rAAV-Ef1α-DIO-N2cG Brain Case Cat# BC-0304 rAAV-Ef1α-DIO-mCherry-F2A-TVA Brain Case Cat# BC-0061 CVS-EnvA-ΔG-EGFP Brain Case Cat# BC-RV-CVS EnVA461 rAAV2/1-hSyn-Cre-WPRE-hGH-pA BrainVTA Cat# PT-0136 rAAV-Ef1α-DIO-mCherry-WPRE-hGH BrainVTA Cat# PT-0795 rAAV-Ef1α-DIO-hChR2 (H134R)-mCherry-WPRE-pA BrainVTA Cat# PT-0002 rAAV-Ef1α-DIO-hM4D(Gi)-EGFP-WPRE-pA BrainVTA Cat# PT-0043 rAAV-Ef1α-DIO-hM3D(Gq)-mCherry-WPRE-pA BrainVTA Cat# PT-0042 retro-rAAV-Ef1α-DIO-hM4D(Gi)-EGFP-WPRE-pA BrainVTA Cat# PT-0987 retro-rAAV-Ef1α-DIO-hM3D(Gq)-mCherry-WPRE-pA BrainVTA Cat# PT-0042 retro-rAAV-hSyn-CRE-WPRE-hGH BrainVTA Cat# PT-0136 retro-rAAV-Ef1α-DIO-EGFP-WPRE-hGH BrainVTA Cat# PT-0795 retro-rAAV-Ef1α-DIO-mCherry-WPRE-hGH BrainVTA Cat# PT-0013 rAAV-Ef1α-DIO-GCaMP6s-WPRE-pA BrainVTA Cat# PT-0071 retro-rAAV-Ef1α-DIO-GCaMP6s-WPRE-pA BrainVTA Cat# PT-0071 rAAV-CAG-DIO-WGA-FLP-WPRE-hGH-pA BrainVTA Cat# PT-0557 rAAV-EF1a-fDIO-mCherry-WPRE-hGH-pA BrainVTA Cat# PT-1046 rAAV-cfos-tTA-WPRE-hGH-pA BrainVTA Cat# PT-3022 rAAV-TRE-tight-EGFP-WPREs BrainVTA Cat# PT-9667 Chemicals, peptides, and recombinant proteins TTX Tauto Biotech Cat# E− 2968 4-AP MCE HY-B0604 CNQX Tocris Cat# 0190 Bicuculine Selleck Cat# S7071 Clozapine N-oxide TargetMol Cat# T4494 Experimental models: other:Article Title: A regulatory circuitry driving a noradrenergic to mesenchymal transition highlights YAP/TAZ as critical players in neuroblastoma plasticity. Article Snippet: SK-N-SHm Thirant et al.12 N/A SH-SY5Y ATCC CRL-2266; RRID: CVCL_0019 SK-N-BE(2)C ATCC CRL-2268; RRID: CVCL_0529 5Y clone MES This paper, Louis-Brennetot, Peltier et al., in preparation N/A CLB-GA Boeva et al.3 RRID:CVCL_9529 SH-EP Boeva et al.3 RRID:CVCL_0524 Experimental models: Organisms/strains 8-week-old Software:Article Title: Giredestrant immobilizes the estrogen receptor to exert potent antitumor efficacy against ER-active breast cancers. Article Snippet: ZR-75-1 ATCC ATCC® CRL-1500TM .. Experimental models: Article Title: SEPHS2 loss reprograms cancer metabolism from oxidative phosphorylation to gluconeogenesis via PCK1 stabilization. Article Snippet: Upon arrival at the animal core facility of Southern University of Science and Technology, mice were acclimated for 1 week in a barrier facility to minimize the potential effects of .. REAGENT or RESOURCE SOURCE IDENTIFIER M&R HRP/DAB Detection IHC Kit Vazyme Cat#HC301-01; Lot#7E2762K4 Deposited data Transcriptomics: A-375 control and SEPHS2 knockout cells This paper GSE:324223 Proteomics:A-375 control and SEPHS2 knockout cells This paper PXD076137 Experimental models: Cell lines A-375 ATCC Cat#CRL-1619 HeLa ATCC Cat#CCL-2 A2058 CellCook Cat#CC1805 HEK-293FT CellCook Cat#CC4046 MCF-7 CellCook Cat#CC0302 Experimental models: Article Title: Distinct supraspinal neural pathways underlie licking-induced alleviation of pain sensation and aversion in male mice. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit Anti-Glutamate antibody Sigma-Aldrich Cat# G6642; RRID: AB_259946 Rabbit Anti-GABA antibody Sigma-Aldrich Cat# A2052; RRID: AB_477652 Rabbit Anti-TH antibody HUABIO Cat# ET1611-12; RRID: AB_3069989 Pig Anti-c-Fos antibody SYSY Cat# 226308; RRID: AB_2905595 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) Abcam Cat# ab150077; RRID: AB_2630356 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555) Abcam Cat# ab150078; RRID: AB_2722519 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) Abcam Cat# ab150079; RRID: AB_2722623 Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 488) Abcam Cat# ab150185; RRID: AB_2736871 Bacterial and virus strains rAAV-Ef1α-DIO-N2cG Brain Case Cat# BC-0304 rAAV-Ef1α-DIO-mCherry-F2A-TVA Brain Case Cat# BC-0061 CVS-EnvA-ΔG-EGFP Brain Case Cat# BC-RV-CVS EnVA461 rAAV2/1-hSyn-Cre-WPRE-hGH-pA BrainVTA Cat# PT-0136 rAAV-Ef1α-DIO-mCherry-WPRE-hGH BrainVTA Cat# PT-0795 rAAV-Ef1α-DIO-hChR2 (H134R)-mCherry-WPRE-pA BrainVTA Cat# PT-0002 rAAV-Ef1α-DIO-hM4D(Gi)-EGFP-WPRE-pA BrainVTA Cat# PT-0043 rAAV-Ef1α-DIO-hM3D(Gq)-mCherry-WPRE-pA BrainVTA Cat# PT-0042 retro-rAAV-Ef1α-DIO-hM4D(Gi)-EGFP-WPRE-pA BrainVTA Cat# PT-0987 retro-rAAV-Ef1α-DIO-hM3D(Gq)-mCherry-WPRE-pA BrainVTA Cat# PT-0042 retro-rAAV-hSyn-CRE-WPRE-hGH BrainVTA Cat# PT-0136 retro-rAAV-Ef1α-DIO-EGFP-WPRE-hGH BrainVTA Cat# PT-0795 retro-rAAV-Ef1α-DIO-mCherry-WPRE-hGH BrainVTA Cat# PT-0013 rAAV-Ef1α-DIO-GCaMP6s-WPRE-pA BrainVTA Cat# PT-0071 retro-rAAV-Ef1α-DIO-GCaMP6s-WPRE-pA BrainVTA Cat# PT-0071 rAAV-CAG-DIO-WGA-FLP-WPRE-hGH-pA BrainVTA Cat# PT-0557 rAAV-EF1a-fDIO-mCherry-WPRE-hGH-pA BrainVTA Cat# PT-1046 rAAV-cfos-tTA-WPRE-hGH-pA BrainVTA Cat# PT-3022 rAAV-TRE-tight-EGFP-WPREs BrainVTA Cat# PT-9667 Chemicals, peptides, and recombinant proteins TTX Tauto Biotech Cat# E− 2968 4-AP MCE HY-B0604 CNQX Tocris Cat# 0190 Bicuculine Selleck Cat# S7071 Clozapine N-oxide TargetMol Cat# T4494 Experimental models: Article Title: Aberrant prefrontal activity and arousal level correlate with action initiation and response vigor in rats Article Snippet: .. 530 531 Key Resources Table 532 REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data Data https://etsin.fairdata.fi / https://doi.org/10.2 3729/fd-fde5397bd01f-368a-9be1a750d5f4d589 Experimental models: Article Title: Perinatal infection elicits clonally restricted T follicular helper cell responses that drive antibody-mediated viral control. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Article Title: Circuit and molecular mechanisms underlying incubation of methamphetamine craving in the prelimbic cortex. Article Snippet: Article Circuit and molecular mechanisms underlying incubation of methamphetamine craving in the prelimbic cortex Highlights • SST and PV interneurons in the PL differentially regulate methamphetamine craving • LHGABA → PLSST and AMGlu → PLPV circuits drive stagespecific craving • KCNC2 upregulation in interneurons underlies distinct neuronal excitability changes • Specific knockdown of KCNC2 reduces craving during early and prolonged withdrawal Authors Sai Shi, Yi-Wen Sun, Jin-Jun Ding, ..., Fang Liu, Ti-Fei Yuan, Min Zhao Correspondence fang.liu@camh.ca (F.L.), ytf0707@126.com (T..-F.Y.), drminzhao@smhc.org.cn (M.Z.). In brief Shi et al. reveal that two distinct interneurons in the PL control the incubation of methamphetamine craving during different withdrawal stages.. They uncover a circuit-switch principle and propose a key molecular mediator, offering phase-specific therapeutic strategies to reduce relapse. Immunohistochemistry:Article Title: SEPHS2 loss reprograms cancer metabolism from oxidative phosphorylation to gluconeogenesis via PCK1 stabilization. Article Snippet: Upon arrival at the animal core facility of Southern University of Science and Technology, mice were acclimated for 1 week in a barrier facility to minimize the potential effects of .. REAGENT or RESOURCE SOURCE IDENTIFIER M&R HRP/DAB Detection IHC Kit Vazyme Cat#HC301-01; Lot#7E2762K4 Deposited data Transcriptomics: A-375 control and SEPHS2 knockout cells This paper GSE:324223 Proteomics:A-375 control and SEPHS2 knockout cells This paper PXD076137 Experimental models: Cell lines A-375 ATCC Cat#CRL-1619 HeLa ATCC Cat#CCL-2 A2058 CellCook Cat#CC1805 HEK-293FT CellCook Cat#CC4046 MCF-7 CellCook Cat#CC0302 Experimental models: Transcriptomics:Article Title: SEPHS2 loss reprograms cancer metabolism from oxidative phosphorylation to gluconeogenesis via PCK1 stabilization. Article Snippet: Upon arrival at the animal core facility of Southern University of Science and Technology, mice were acclimated for 1 week in a barrier facility to minimize the potential effects of .. REAGENT or RESOURCE SOURCE IDENTIFIER M&R HRP/DAB Detection IHC Kit Vazyme Cat#HC301-01; Lot#7E2762K4 Deposited data Transcriptomics: A-375 control and SEPHS2 knockout cells This paper GSE:324223 Proteomics:A-375 control and SEPHS2 knockout cells This paper PXD076137 Experimental models: Cell lines A-375 ATCC Cat#CRL-1619 HeLa ATCC Cat#CCL-2 A2058 CellCook Cat#CC1805 HEK-293FT CellCook Cat#CC4046 MCF-7 CellCook Cat#CC0302 Experimental models: Control:Article Title: SEPHS2 loss reprograms cancer metabolism from oxidative phosphorylation to gluconeogenesis via PCK1 stabilization. Article Snippet: Upon arrival at the animal core facility of Southern University of Science and Technology, mice were acclimated for 1 week in a barrier facility to minimize the potential effects of .. REAGENT or RESOURCE SOURCE IDENTIFIER M&R HRP/DAB Detection IHC Kit Vazyme Cat#HC301-01; Lot#7E2762K4 Deposited data Transcriptomics: A-375 control and SEPHS2 knockout cells This paper GSE:324223 Proteomics:A-375 control and SEPHS2 knockout cells This paper PXD076137 Experimental models: Cell lines A-375 ATCC Cat#CRL-1619 HeLa ATCC Cat#CCL-2 A2058 CellCook Cat#CC1805 HEK-293FT CellCook Cat#CC4046 MCF-7 CellCook Cat#CC0302 Experimental models: Knock-Out:Article Title: SEPHS2 loss reprograms cancer metabolism from oxidative phosphorylation to gluconeogenesis via PCK1 stabilization. Article Snippet: Upon arrival at the animal core facility of Southern University of Science and Technology, mice were acclimated for 1 week in a barrier facility to minimize the potential effects of .. REAGENT or RESOURCE SOURCE IDENTIFIER M&R HRP/DAB Detection IHC Kit Vazyme Cat#HC301-01; Lot#7E2762K4 Deposited data Transcriptomics: A-375 control and SEPHS2 knockout cells This paper GSE:324223 Proteomics:A-375 control and SEPHS2 knockout cells This paper PXD076137 Experimental models: Cell lines A-375 ATCC Cat#CRL-1619 HeLa ATCC Cat#CCL-2 A2058 CellCook Cat#CC1805 HEK-293FT CellCook Cat#CC4046 MCF-7 CellCook Cat#CC0302 Experimental models: shRNA:Article Title: SEPHS2 loss reprograms cancer metabolism from oxidative phosphorylation to gluconeogenesis via PCK1 stabilization. Article Snippet: Upon arrival at the animal core facility of Southern University of Science and Technology, mice were acclimated for 1 week in a barrier facility to minimize the potential effects of .. REAGENT or RESOURCE SOURCE IDENTIFIER M&R HRP/DAB Detection IHC Kit Vazyme Cat#HC301-01; Lot#7E2762K4 Deposited data Transcriptomics: A-375 control and SEPHS2 knockout cells This paper GSE:324223 Proteomics:A-375 control and SEPHS2 knockout cells This paper PXD076137 Experimental models: Cell lines A-375 ATCC Cat#CRL-1619 HeLa ATCC Cat#CCL-2 A2058 CellCook Cat#CC1805 HEK-293FT CellCook Cat#CC4046 MCF-7 CellCook Cat#CC0302 Experimental models: Real-time Polymerase Chain Reaction:Article Title: SEPHS2 loss reprograms cancer metabolism from oxidative phosphorylation to gluconeogenesis via PCK1 stabilization. Article Snippet: Upon arrival at the animal core facility of Southern University of Science and Technology, mice were acclimated for 1 week in a barrier facility to minimize the potential effects of .. REAGENT or RESOURCE SOURCE IDENTIFIER M&R HRP/DAB Detection IHC Kit Vazyme Cat#HC301-01; Lot#7E2762K4 Deposited data Transcriptomics: A-375 control and SEPHS2 knockout cells This paper GSE:324223 Proteomics:A-375 control and SEPHS2 knockout cells This paper PXD076137 Experimental models: Cell lines A-375 ATCC Cat#CRL-1619 HeLa ATCC Cat#CCL-2 A2058 CellCook Cat#CC1805 HEK-293FT CellCook Cat#CC4046 MCF-7 CellCook Cat#CC0302 Experimental models: Article Title: Circuit and molecular mechanisms underlying incubation of methamphetamine craving in the prelimbic cortex. Article Snippet: Article Circuit and molecular mechanisms underlying incubation of methamphetamine craving in the prelimbic cortex Highlights • SST and PV interneurons in the PL differentially regulate methamphetamine craving • LHGABA → PLSST and AMGlu → PLPV circuits drive stagespecific craving • KCNC2 upregulation in interneurons underlies distinct neuronal excitability changes • Specific knockdown of KCNC2 reduces craving during early and prolonged withdrawal Authors Sai Shi, Yi-Wen Sun, Jin-Jun Ding, ..., Fang Liu, Ti-Fei Yuan, Min Zhao Correspondence fang.liu@camh.ca (F.L.), ytf0707@126.com (T..-F.Y.), drminzhao@smhc.org.cn (M.Z.). In brief Shi et al. reveal that two distinct interneurons in the PL control the incubation of methamphetamine craving during different withdrawal stages.. They uncover a circuit-switch principle and propose a key molecular mediator, offering phase-specific therapeutic strategies to reduce relapse. Virus:Article Title: Distinct supraspinal neural pathways underlie licking-induced alleviation of pain sensation and aversion in male mice. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit Anti-Glutamate antibody Sigma-Aldrich Cat# G6642; RRID: AB_259946 Rabbit Anti-GABA antibody Sigma-Aldrich Cat# A2052; RRID: AB_477652 Rabbit Anti-TH antibody HUABIO Cat# ET1611-12; RRID: AB_3069989 Pig Anti-c-Fos antibody SYSY Cat# 226308; RRID: AB_2905595 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) Abcam Cat# ab150077; RRID: AB_2630356 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555) Abcam Cat# ab150078; RRID: AB_2722519 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) Abcam Cat# ab150079; RRID: AB_2722623 Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 488) Abcam Cat# ab150185; RRID: AB_2736871 Bacterial and virus strains rAAV-Ef1α-DIO-N2cG Brain Case Cat# BC-0304 rAAV-Ef1α-DIO-mCherry-F2A-TVA Brain Case Cat# BC-0061 CVS-EnvA-ΔG-EGFP Brain Case Cat# BC-RV-CVS EnVA461 rAAV2/1-hSyn-Cre-WPRE-hGH-pA BrainVTA Cat# PT-0136 rAAV-Ef1α-DIO-mCherry-WPRE-hGH BrainVTA Cat# PT-0795 rAAV-Ef1α-DIO-hChR2 (H134R)-mCherry-WPRE-pA BrainVTA Cat# PT-0002 rAAV-Ef1α-DIO-hM4D(Gi)-EGFP-WPRE-pA BrainVTA Cat# PT-0043 rAAV-Ef1α-DIO-hM3D(Gq)-mCherry-WPRE-pA BrainVTA Cat# PT-0042 retro-rAAV-Ef1α-DIO-hM4D(Gi)-EGFP-WPRE-pA BrainVTA Cat# PT-0987 retro-rAAV-Ef1α-DIO-hM3D(Gq)-mCherry-WPRE-pA BrainVTA Cat# PT-0042 retro-rAAV-hSyn-CRE-WPRE-hGH BrainVTA Cat# PT-0136 retro-rAAV-Ef1α-DIO-EGFP-WPRE-hGH BrainVTA Cat# PT-0795 retro-rAAV-Ef1α-DIO-mCherry-WPRE-hGH BrainVTA Cat# PT-0013 rAAV-Ef1α-DIO-GCaMP6s-WPRE-pA BrainVTA Cat# PT-0071 retro-rAAV-Ef1α-DIO-GCaMP6s-WPRE-pA BrainVTA Cat# PT-0071 rAAV-CAG-DIO-WGA-FLP-WPRE-hGH-pA BrainVTA Cat# PT-0557 rAAV-EF1a-fDIO-mCherry-WPRE-hGH-pA BrainVTA Cat# PT-1046 rAAV-cfos-tTA-WPRE-hGH-pA BrainVTA Cat# PT-3022 rAAV-TRE-tight-EGFP-WPREs BrainVTA Cat# PT-9667 Chemicals, peptides, and recombinant proteins TTX Tauto Biotech Cat# E− 2968 4-AP MCE HY-B0604 CNQX Tocris Cat# 0190 Bicuculine Selleck Cat# S7071 Clozapine N-oxide TargetMol Cat# T4494 Experimental models: SYBR Green Assay:Article Title: Circuit and molecular mechanisms underlying incubation of methamphetamine craving in the prelimbic cortex. Article Snippet: Article Circuit and molecular mechanisms underlying incubation of methamphetamine craving in the prelimbic cortex Highlights • SST and PV interneurons in the PL differentially regulate methamphetamine craving • LHGABA → PLSST and AMGlu → PLPV circuits drive stagespecific craving • KCNC2 upregulation in interneurons underlies distinct neuronal excitability changes • Specific knockdown of KCNC2 reduces craving during early and prolonged withdrawal Authors Sai Shi, Yi-Wen Sun, Jin-Jun Ding, ..., Fang Liu, Ti-Fei Yuan, Min Zhao Correspondence fang.liu@camh.ca (F.L.), ytf0707@126.com (T..-F.Y.), drminzhao@smhc.org.cn (M.Z.). In brief Shi et al. reveal that two distinct interneurons in the PL control the incubation of methamphetamine craving during different withdrawal stages.. They uncover a circuit-switch principle and propose a key molecular mediator, offering phase-specific therapeutic strategies to reduce relapse. Multiplex Assay:Article Title: Circuit and molecular mechanisms underlying incubation of methamphetamine craving in the prelimbic cortex. Article Snippet: Article Circuit and molecular mechanisms underlying incubation of methamphetamine craving in the prelimbic cortex Highlights • SST and PV interneurons in the PL differentially regulate methamphetamine craving • LHGABA → PLSST and AMGlu → PLPV circuits drive stagespecific craving • KCNC2 upregulation in interneurons underlies distinct neuronal excitability changes • Specific knockdown of KCNC2 reduces craving during early and prolonged withdrawal Authors Sai Shi, Yi-Wen Sun, Jin-Jun Ding, ..., Fang Liu, Ti-Fei Yuan, Min Zhao Correspondence fang.liu@camh.ca (F.L.), ytf0707@126.com (T..-F.Y.), drminzhao@smhc.org.cn (M.Z.). In brief Shi et al. reveal that two distinct interneurons in the PL control the incubation of methamphetamine craving during different withdrawal stages.. They uncover a circuit-switch principle and propose a key molecular mediator, offering phase-specific therapeutic strategies to reduce relapse. Protease Inhibitor:Article Title: Circuit and molecular mechanisms underlying incubation of methamphetamine craving in the prelimbic cortex. Article Snippet: Article Circuit and molecular mechanisms underlying incubation of methamphetamine craving in the prelimbic cortex Highlights • SST and PV interneurons in the PL differentially regulate methamphetamine craving • LHGABA → PLSST and AMGlu → PLPV circuits drive stagespecific craving • KCNC2 upregulation in interneurons underlies distinct neuronal excitability changes • Specific knockdown of KCNC2 reduces craving during early and prolonged withdrawal Authors Sai Shi, Yi-Wen Sun, Jin-Jun Ding, ..., Fang Liu, Ti-Fei Yuan, Min Zhao Correspondence fang.liu@camh.ca (F.L.), ytf0707@126.com (T..-F.Y.), drminzhao@smhc.org.cn (M.Z.). In brief Shi et al. reveal that two distinct interneurons in the PL control the incubation of methamphetamine craving during different withdrawal stages.. They uncover a circuit-switch principle and propose a key molecular mediator, offering phase-specific therapeutic strategies to reduce relapse. Lysis:Article Title: Circuit and molecular mechanisms underlying incubation of methamphetamine craving in the prelimbic cortex. Article Snippet: Article Circuit and molecular mechanisms underlying incubation of methamphetamine craving in the prelimbic cortex Highlights • SST and PV interneurons in the PL differentially regulate methamphetamine craving • LHGABA → PLSST and AMGlu → PLPV circuits drive stagespecific craving • KCNC2 upregulation in interneurons underlies distinct neuronal excitability changes • Specific knockdown of KCNC2 reduces craving during early and prolonged withdrawal Authors Sai Shi, Yi-Wen Sun, Jin-Jun Ding, ..., Fang Liu, Ti-Fei Yuan, Min Zhao Correspondence fang.liu@camh.ca (F.L.), ytf0707@126.com (T..-F.Y.), drminzhao@smhc.org.cn (M.Z.). In brief Shi et al. reveal that two distinct interneurons in the PL control the incubation of methamphetamine craving during different withdrawal stages.. They uncover a circuit-switch principle and propose a key molecular mediator, offering phase-specific therapeutic strategies to reduce relapse. Bicinchoninic Acid Protein Assay:Article Title: Circuit and molecular mechanisms underlying incubation of methamphetamine craving in the prelimbic cortex. Article Snippet: Article Circuit and molecular mechanisms underlying incubation of methamphetamine craving in the prelimbic cortex Highlights • SST and PV interneurons in the PL differentially regulate methamphetamine craving • LHGABA → PLSST and AMGlu → PLPV circuits drive stagespecific craving • KCNC2 upregulation in interneurons underlies distinct neuronal excitability changes • Specific knockdown of KCNC2 reduces craving during early and prolonged withdrawal Authors Sai Shi, Yi-Wen Sun, Jin-Jun Ding, ..., Fang Liu, Ti-Fei Yuan, Min Zhao Correspondence fang.liu@camh.ca (F.L.), ytf0707@126.com (T..-F.Y.), drminzhao@smhc.org.cn (M.Z.). In brief Shi et al. reveal that two distinct interneurons in the PL control the incubation of methamphetamine craving during different withdrawal stages.. They uncover a circuit-switch principle and propose a key molecular mediator, offering phase-specific therapeutic strategies to reduce relapse. |