one step tunel apoptosis assay kit (Beyotime)

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Beyotime one step tunel apoptosis assay kit

Fig. 4 AGH improved glutathione metabolism and reduced <t>apoptosis.</t> TA muscles from WT, mdx, and AGH-treatment mdx mice were isolated at 8 weeks of age. The ratio of (A) oxidized glutathione (GSSG) to glutathione (GSH) (GSSG/GSH), (B) the concentration of total GSH, (C) GSSG, and (D) GSH were mea sured per assay kit instructions. The activities of (E) GSH reductase and (F) GSH peroxidase in TA muscle were measured per assay kit instructions. Gene expression of (G) Gpx2, Gpx3, Gpx4, and (J) caspase3 measured using real-time PCR. Values were normalized to 36B4 levels. (H–I) Representative images and quantification of <t>TUNEL-positive</t> nuclei on TA muscle (20× magnification, scale bar = 100 μm). Values are presented as the mean ± standard error of the mean for each experiment (n = 5). * p < 0.05 compared with WT control, # p < 0.05 compared with mdx

One Step Tunel Apoptosis Assay Kit, supplied by Beyotime, used in various techniques. Bioz Stars score: 98/100, based on 3731 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/image+processing+tool+kit/One+Step+TUNEL+Apoptosis+Assay+Kit/pm39806512-97-9-16
Average 98 stars, based on 3731 article reviews

one step tunel apoptosis assay kit - by Bioz Stars, 2026-09

98/100 stars

Images

1) Product Images from "Aminoguanidine hemisulfate improves mitochondrial autophagy, oxidative stress, and muscle force in Duchenne muscular dystrophy via the AKT/FOXO1 pathway in mdx mice."

Article Title: Aminoguanidine hemisulfate improves mitochondrial autophagy, oxidative stress, and muscle force in Duchenne muscular dystrophy via the AKT/FOXO1 pathway in mdx mice.

Journal: Skeletal muscle

doi: 10.1186/s13395-024-00371-1

Fig. 4 AGH improved glutathione metabolism and reduced apoptosis. TA muscles from WT, mdx, and AGH-treatment mdx mice were isolated at 8 weeks of age. The ratio of (A) oxidized glutathione (GSSG) to glutathione (GSH) (GSSG/GSH), (B) the concentration of total GSH, (C) GSSG, and (D) GSH were mea sured per assay kit instructions. The activities of (E) GSH reductase and (F) GSH peroxidase in TA muscle were measured per assay kit instructions. Gene expression of (G) Gpx2, Gpx3, Gpx4, and (J) caspase3 measured using real-time PCR. Values were normalized to 36B4 levels. (H–I) Representative images and quantification of TUNEL-positive nuclei on TA muscle (20× magnification, scale bar = 100 μm). Values are presented as the mean ± standard error of the mean for each experiment (n = 5). * p < 0.05 compared with WT control, # p < 0.05 compared with mdx

Figure Legend Snippet: Fig. 4 AGH improved glutathione metabolism and reduced apoptosis. TA muscles from WT, mdx, and AGH-treatment mdx mice were isolated at 8 weeks of age. The ratio of (A) oxidized glutathione (GSSG) to glutathione (GSH) (GSSG/GSH), (B) the concentration of total GSH, (C) GSSG, and (D) GSH were mea sured per assay kit instructions. The activities of (E) GSH reductase and (F) GSH peroxidase in TA muscle were measured per assay kit instructions. Gene expression of (G) Gpx2, Gpx3, Gpx4, and (J) caspase3 measured using real-time PCR. Values were normalized to 36B4 levels. (H–I) Representative images and quantification of TUNEL-positive nuclei on TA muscle (20× magnification, scale bar = 100 μm). Values are presented as the mean ± standard error of the mean for each experiment (n = 5). * p < 0.05 compared with WT control, # p < 0.05 compared with mdx

Techniques Used: Muscles, Isolation, Concentration Assay, Gene Expression, Real-time Polymerase Chain Reaction, TUNEL Assay, Control

Fig. 8 Summary diagram of AGH treatment in mdx mice. AGH treatment reduced ROS and apoptosis by restoring mitochondrial autophagy, restored the composition of muscle fiber types, and improved muscle force through the AKT/FOXO1 signaling pathway

Figure Legend Snippet: Fig. 8 Summary diagram of AGH treatment in mdx mice. AGH treatment reduced ROS and apoptosis by restoring mitochondrial autophagy, restored the composition of muscle fiber types, and improved muscle force through the AKT/FOXO1 signaling pathway

Techniques Used:

TUNEL Assay:

Article Title: SLC25A48 promotes colorectal cancer growth by enhancing mitochondrial respiration and conferring ferroptosis resistance.
Article Snippet: Accumulating evidence indicates that mitochondrial dysfunction is a hallmark of cancer.. Nonetheless, the mechanisms linking mitochondrial dysfunction to cancer progression remain largely elusive.. SLC25A48 was recently recognized as a transporter involved in mitochondrial choline uptake.

Article Title: Pressurized intraperitoneal cold atmospheric plasma as a novel therapeutic strategy for peritoneal metastatic carcinoma
Article Snippet: After deparaffinization and antigen retrieval (citrate, 95 °C), sections were permeabilized with Triton X-100(Sigma, X100), blocked with 5 % serum (Invitrogen, 50197Z), and incubated overnight at 4 °C with primary antibodies against Bcl2, Bax, cleaved Caspase 3, and Caspase 9 (1:100–1:200 dilutions). .. Fluorescent secondary antibodies (Alexa Fluor‐488‐, Cy‐3‐, or Alexa Fluor‐647‐conjugated; Invitrogen, A11078, A10521, and A21244) were applied for 1 h, followed by TUNEL staining (One Step TUNEL Apoptosis Assay Kit, Beyotime, C1086) to detect apoptosis. ..

Article Title: Therapeutic Potential of Hypoxia-Preconditioned hiPSC-Epicardial Cell-Derived Exosomes in Mice with Myocardial Infarction
Article Snippet: .. To evaluate apoptosis post-MI, a one-step TUNEL Apoptosis Assay Kit (Beyotime, C1089) was used. ..

Article Title: Spleen-targeted neoantigen mRNA vaccine induces ISG15 + CD8 + T cell-mediated tertiary lymphoid structure formation in hepatocellular carcinoma.
Article Snippet: Hematoxylin and eosin (H&E) staining was performed using H&E Stain Kit (Solarbio) for histopathology analysis. .. Ki67 immunohistochemistry (Servicebio) and TUNEL assays (One Step TUNEL Apoptosis Assay Kit, Beyotime) were conducted to evaluate tumor cell proliferation and apoptosis. .. For two-color immunofluorescence, sections were stained with anti-CD4 (Servicebio) and anti-CD8 (Servicebio) antibodies to visualize intratumoral T cell infiltration.

Article Title: Senolytics alleviate cyclophosphamide-induced premature ovarian insufficiency by eliminating senescent cells.
Article Snippet: Fluorescence images were obtained through the use of a Leica K5 (Germany) microscope, and the ratio of red to green fluorescence intensity was subsequently analyzed using ImageJ software. .. Cell apoptosis detection using TUNEL Before staining, ovarian sections underwent dewaxing and hydration processes as per the instructions provided in the manual for the Colorimetric TUNEL Apoptosis Assay Kit (G1507; Servicebio) conducted an evaluation of apoptosis using the One Step TUNEL Apoptosis Kit (C1089; Beyotime) following the manufacturer’s guidelines. .. To quantify the results, the counts of TUNEL-positive and total granulosa cells in antral follicles were measured with ImageJ software.

Article Title: ZEB1 maintains mitochondrial fission and macrophage efferocytosis by restraining MFN2, thereby limiting inflammation and improving tendon-bone healing
Article Snippet: .. Apoptosis detection was carried out using the One-Step TUNEL Apoptosis Assay Kit (cat. no. C1086; Beyotime Biotechnology), following the manufacturer's instructions. ..

Article Title: Ameliorating post-infarction myocardial fibrosis and cardiac function via ROS-responsive hydrogel-mediated IL-11 antibody delivery
Article Snippet: .. Sections were fixed in 4 % PFA for 30 min, permeabilized, and blocked with DAKO solution (0.1 % saponin) for 1 h. Apoptosis was detected using the One Step TUNEL Apoptosis Kit (Beyotime, C1089) per manufacturer's instructions. .. For immunostaining of biomarkers including Ki67 (CST, 9129), CD31 (Abcam, ab222783), vWF (Abcam, ab31630), α-SMA (Abcam, ab7817), Collagen I (CST, 66948), and Collagen III (Abcam, ab6310), samples were incubated overnight at 4 °C with primary antibodies diluted in blocking buffer.

Staining:

Apoptosis Assay:

Article Title: Spleen-targeted neoantigen mRNA vaccine induces ISG15 + CD8 + T cell-mediated tertiary lymphoid structure formation in hepatocellular carcinoma.
Article Snippet: Hematoxylin and eosin (H&E) staining was performed using H&E Stain Kit (Solarbio) for histopathology analysis. .. Ki67 immunohistochemistry (Servicebio) and TUNEL assays (One Step TUNEL Apoptosis Assay Kit, Beyotime) were conducted to evaluate tumor cell proliferation and apoptosis. .. For two-color immunofluorescence, sections were stained with anti-CD4 (Servicebio) and anti-CD8 (Servicebio) antibodies to visualize intratumoral T cell infiltration.

Immunohistochemistry:

Article Title: Spleen-targeted neoantigen mRNA vaccine induces ISG15 + CD8 + T cell-mediated tertiary lymphoid structure formation in hepatocellular carcinoma.
Article Snippet: Hematoxylin and eosin (H&E) staining was performed using H&E Stain Kit (Solarbio) for histopathology analysis. .. Ki67 immunohistochemistry (Servicebio) and TUNEL assays (One Step TUNEL Apoptosis Assay Kit, Beyotime) were conducted to evaluate tumor cell proliferation and apoptosis. .. For two-color immunofluorescence, sections were stained with anti-CD4 (Servicebio) and anti-CD8 (Servicebio) antibodies to visualize intratumoral T cell infiltration.

other:

Article Title: Spleen-targeted neoantigen mRNA vaccine induces ISG15 + CD8 + T cell-mediated tertiary lymphoid structure formation in hepatocellular carcinoma.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER CD4 (TSA-mIF, rabbit mAb) AiFang Biological Cat#AFRM0003; RRID: N/A CD19 (TSA-mIF, rabbit mAb) AiFang Biological Cat#AFRM0354; RRID: N/A CD20 (TSA-mIF, rabbit mAb) Abcam Cat#ab64088; RRID:AB_1139386 CD23 (TSA-mIF, rabbit mAb) Abcam Cat#ab315289; RRID: N/A CD21 (TSA-mIF, rabbit mAb) Abcam Cat#ab227662; RRID: N/A CD34 (TSA-mIF, rabbit mAb) AiFang Biological Cat#AFRM0080; RRID: N/A Bcl6 (TSA-mIF, rabbit pAb) Invitrogen Cat#PA5-27390; RRID:AB_2544866 CD138 (TSA-mIF, rabbit pAb) Proteintech Cat#10593-1-AP; RRID:AB_2182736 CD27 (TSA-mIF, rabbit mAb) Abcam Cat#ab214043; RRID: N/A CD86 (TSA-mIF, rabbit mAb) AiFang Biological Cat#AFRM0167; RRID: N/A Chemicals, peptides, and recombinant proteins DOTMA Sinopeg Cat#06040009700 DOPE Sinopeg Cat#06030000500 SM-102 Sinopeg Cat#06040008800 DSPC Sinopeg Cat#06030001100 cholesterol Sinopeg Cat#06040015200 DMG-PEG2000 AVT Cat#O02005 HindIII restriction enzyme Thermo-Fisher Cat#FD0505 Luciferin substrate PerkinElmer Cat#122799 Heparin sodium Achem-block Cat#ABC-B132998 SCH79797 (F2R antagonist, F2RA) MCE Cat#HY-14994 Recombinant GZMA MCE Cat#HY-P76377 OKT3 T&L biotechnology Cat#GMP-TL101-0500 Trypan Blue Solution Gibco Cat#15250-061 Critical commercial assays Mouse IFN-γ ELISpot Kit Mabtech Cat#3321-4AST-2 H&E Stain Kit Solarbio Cat#G1120 One Step TUNEL Apoptosis Assay Kit Beyotime Cat#C1086 Mouse IL-12 ELISA kit Boster Bio Cat#EK0422 Mouse IFN-γ ELISA kit Boster Bio Cat# EK0375 Mouse IgG ELISA kit Boster Bio Cat#EK0101 Mouse IL-1β ELISA kit Boster Bio Cat#EK0394 Mouse IL-6 ELISA kit Boster Bio Cat#EK0411 Mouse TNF-α ELISA kit Boster Bio Cat#EK0527 Mouse IFN-β ELISA kit FANKEW Cat#F2124-A Mouse C3a ELISA kit Cusabio Biotech Cat#CSB-E08666r Mouse C5a ELISA kit Cusabio Biotech Cat#CSB-E14363r Fixation/Permeabilization Solution Kit BD Bioscience Cat#554715 HyperScribe TM All in One mRNA Synthesis Kit Plus 1 APExBIO Cat#K1064 HyperScribe TM T7 High Yield Cy5 RNA Labeling Kit APExBIO Cat#K1063 VAHTS RNA Clean Beads Vazyme Cat#N412-01 Cell Counting Kit-8 Dojindo Laboratories Cat#CK04-01 sCelLive Tissue Digestion Solution Singleron Cat#1020012 FocuSCOPE Single Cell Multiomics mRNA × Mouse Liver Mutation Detection Kit Singleron Cat#4212122 GEXSCOPE Single Cell RNA Library Kit Singleron Cat#4180022 DynaSpatial FFPE Spatial Gene Expression Reagents Kit (for Mouse Transcriptome) Dynamic Biosystems Cat#13010060 (Continued on next page) Cell Reports Medicine 7, 102754, May 19, 2026 e2 OPEN ACCESS

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