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rneasy kit  (Qiagen)


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    Structured Review

    Qiagen rneasy kit
    Rneasy Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 4497 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+transcriptome+array+(hta)+microarray+platform/RNeasy+FFPE+Kit/pmc03152464-138-8-10
    Average 99 stars, based on 4497 article reviews
    rneasy kit - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    RNA Extraction:

    Article Title: A Spatial Atlas of Muscle-Invasive Bladder Cancer Reveals Lineage-Specific Vulnerabilities and Immune Architecture
    Article Snippet: 766 ST library preparation and sequencing 767 Spatial transcriptomics (ST) analysis on FFPE slides were performed with the Visium spatial 768 technology from 10x Genomics. .. Two to three consecutive tissue sections of 5-μm thickness were 769 collected for RNA extraction with the Qiagen RNeasy FFPE Kit to assess the RNA quality of the 770 tissue. ..

    Article Title: Nanosystems as selective vehicles
    Article Snippet: .. To do this, we performed RNA extraction from 5 paraffin sections of 14 microns each using a specific extraction kit for paraffinized samples (RNeasy FFPE kit, QIAGEN). .. In order to know the concentration of it, the Nanodrop equipment (Nanodrop 2000C, Thermoscientific) was used.

    Formalin-fixed Paraffin-Embedded:

    Article Title: A Spatial Atlas of Muscle-Invasive Bladder Cancer Reveals Lineage-Specific Vulnerabilities and Immune Architecture
    Article Snippet: 766 ST library preparation and sequencing 767 Spatial transcriptomics (ST) analysis on FFPE slides were performed with the Visium spatial 768 technology from 10x Genomics. .. Two to three consecutive tissue sections of 5-μm thickness were 769 collected for RNA extraction with the Qiagen RNeasy FFPE Kit to assess the RNA quality of the 770 tissue. ..

    Article Title: Comprehensive analysis of six MED15::TFE3 renal cell carcinomas including two novel solid MED15::TFE3 renal cell carcinomas, highlighting their morphologic, immunohistochemical and molecular differences from their cystic counterparts.
    Article Snippet: .. RNA was extracted using the RNeasy FFPE Kit (Qiagen, Valencia, CA) and quantified using a Qubit fluorometric assay (Thermo Fisher Scientific, FosterCity, CA) adjusted for the percentage of fragments greater than 100 bp using the TapeStation system (Agilent). .. 300 ng RNA was subjected to library prep using the KAPA stranded RNA-Seq Kit with RiboErase (Kapa Biosystems), followed by quantitation using the KAPA library quantification kit (Kapa Biosystems).

    Article Title: Spatial transcriptomic profiling of decalcified murine musculoskeletal samples via Xenium Prime 5K
    Article Snippet: .. RNA was isolated from tissue sections using the RNeasy FFPE isolation kit (Qiagen; 73504). ..

    Article Title: Spatial Proximity Between PD-L1(+) Tumor-Associated Macrophages and CD8(+) T Cells Influences Response to Atezolizumab Plus Bevacizumab in Hepatocellular Carcinoma.
    Article Snippet: .. Total RNA was extracted using the RNeasy FFPE Kit (QIAGEN, Hilden, Germany). .. RNA quantity and quality were assessed using a Qubit 3.0 Fluorometer with the RNA HS Assay (Thermo Fisher Scientific, Waltham, MA, USA), and RNA integrity was evaluated using the Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA, USA).

    Article Title: Microarray Gene Expression Analysis of Lesional Skin in Canine Vesicular Cutaneous Lupus Erythematosus (VCLE).
    Article Snippet: Background: Vesicular cutaneous lupus erythematosus (VCLE) is a rare autoimmune disease in dogs and is considered the canine counterpart of human subacute cutaneous lupus erythematosus (SCLE).. However, the molecular mechanisms underlying VCLE remain incompletely defined.. Objective/Hypothesis: To characterise gene expression changes in lesional skin from dogs with VCLE and identify immune pathways involved in disease pathogenesis.

    Article Title: Chimeric antigen receptor T cell therapy
    Article Snippet: .. Gene Expression Analysis and Immunosign RNA was extracted from frozen or fixed biopsies using QIAGEN RNeasy kit or QIAGEN RNeasy FFPE extraction kit, respectively. ..

    Article Title: Preanalytical Determinants of DNA and RNA Quality in FFPE Tissues: Practical Recommendations for Molecular Testing.
    Article Snippet: FFPE tissue is essential for molecular testing, but nucleic acid quality is affected by preanalytical factors.. Using porcine models, we systematically examined how formalin concentration, fixation duration, cold ischemia time, and tissue size affect DNA and RNA quality.. Fresh liver was trimmed to 2, 10, or 50 mm and fixed in 10% or 20% neutral‐buffered formalin (NBF) for 1, 3, or 7 days.

    Article Title: Nanosystems as selective vehicles
    Article Snippet: .. To do this, we performed RNA extraction from 5 paraffin sections of 14 microns each using a specific extraction kit for paraffinized samples (RNeasy FFPE kit, QIAGEN). .. In order to know the concentration of it, the Nanodrop equipment (Nanodrop 2000C, Thermoscientific) was used.

    Isolation:

    Article Title: Spatial transcriptomic profiling of decalcified murine musculoskeletal samples via Xenium Prime 5K
    Article Snippet: .. RNA was isolated from tissue sections using the RNeasy FFPE isolation kit (Qiagen; 73504). ..

    Article Title: Microarray Gene Expression Analysis of Lesional Skin in Canine Vesicular Cutaneous Lupus Erythematosus (VCLE).
    Article Snippet: Background: Vesicular cutaneous lupus erythematosus (VCLE) is a rare autoimmune disease in dogs and is considered the canine counterpart of human subacute cutaneous lupus erythematosus (SCLE).. However, the molecular mechanisms underlying VCLE remain incompletely defined.. Objective/Hypothesis: To characterise gene expression changes in lesional skin from dogs with VCLE and identify immune pathways involved in disease pathogenesis.

    Gene Expression:

    Article Title: Chimeric antigen receptor T cell therapy
    Article Snippet: .. Gene Expression Analysis and Immunosign RNA was extracted from frozen or fixed biopsies using QIAGEN RNeasy kit or QIAGEN RNeasy FFPE extraction kit, respectively. ..

    Extraction:

    Article Title: Chimeric antigen receptor T cell therapy
    Article Snippet: .. Gene Expression Analysis and Immunosign RNA was extracted from frozen or fixed biopsies using QIAGEN RNeasy kit or QIAGEN RNeasy FFPE extraction kit, respectively. ..

    Article Title: Nanosystems as selective vehicles
    Article Snippet: .. To do this, we performed RNA extraction from 5 paraffin sections of 14 microns each using a specific extraction kit for paraffinized samples (RNeasy FFPE kit, QIAGEN). .. In order to know the concentration of it, the Nanodrop equipment (Nanodrop 2000C, Thermoscientific) was used.

    Concentration Assay:

    Article Title: Preanalytical Determinants of DNA and RNA Quality in FFPE Tissues: Practical Recommendations for Molecular Testing.
    Article Snippet: FFPE tissue is essential for molecular testing, but nucleic acid quality is affected by preanalytical factors.. Using porcine models, we systematically examined how formalin concentration, fixation duration, cold ischemia time, and tissue size affect DNA and RNA quality.. Fresh liver was trimmed to 2, 10, or 50 mm and fixed in 10% or 20% neutral‐buffered formalin (NBF) for 1, 3, or 7 days.



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    Individual heat-map and PCA analysis of five individual subjects show similar trends but potentially retained some degree of variability in temporal induction and magnitude between donors. (A) Individual heat-maps of different subjects revealed largely similar patterns with potential differences in temporal induction from 8 to 48 hpi, including the magnitude of induction. (B) Individual PCA plot showed that the <t>transcriptomes</t> across different subjects were not clustered as tightly since the hNEC samples were of different genetic backgrounds. The PCA plot showed a distinct progression of differential gene changes across time-points even at 0 hpi. (C) Individual viral titers increased over time to reach high viral loads in samples from all donors. The individual variation was deemed not clinically significant given that a sufficiently high virus titer was attained to induce a consistent response.
    Human Transcriptome Array (Hta) Microarray Platform, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 90 stars, based on 1 article reviews
    human transcriptome array (hta) microarray platform - by Bioz Stars, 2026-09
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    Image Search Results


    Individual heat-map and PCA analysis of five individual subjects show similar trends but potentially retained some degree of variability in temporal induction and magnitude between donors. (A) Individual heat-maps of different subjects revealed largely similar patterns with potential differences in temporal induction from 8 to 48 hpi, including the magnitude of induction. (B) Individual PCA plot showed that the transcriptomes across different subjects were not clustered as tightly since the hNEC samples were of different genetic backgrounds. The PCA plot showed a distinct progression of differential gene changes across time-points even at 0 hpi. (C) Individual viral titers increased over time to reach high viral loads in samples from all donors. The individual variation was deemed not clinically significant given that a sufficiently high virus titer was attained to induce a consistent response.

    Journal: Frontiers in Microbiology

    Article Title: Comparative Transcriptomic and Metagenomic Analyses of Influenza Virus-Infected Nasal Epithelial Cells From Multiple Individuals Reveal Specific Nasal-Initiated Signatures

    doi: 10.3389/fmicb.2018.02685

    Figure Lengend Snippet: Individual heat-map and PCA analysis of five individual subjects show similar trends but potentially retained some degree of variability in temporal induction and magnitude between donors. (A) Individual heat-maps of different subjects revealed largely similar patterns with potential differences in temporal induction from 8 to 48 hpi, including the magnitude of induction. (B) Individual PCA plot showed that the transcriptomes across different subjects were not clustered as tightly since the hNEC samples were of different genetic backgrounds. The PCA plot showed a distinct progression of differential gene changes across time-points even at 0 hpi. (C) Individual viral titers increased over time to reach high viral loads in samples from all donors. The individual variation was deemed not clinically significant given that a sufficiently high virus titer was attained to induce a consistent response.

    Article Snippet: Gene microarrays for the infected and control hNEC samples were performed using Affymetrix human transcriptome array (HTA) microarray platform.

    Techniques: