Review



rneasy mini kit  (Qiagen)


Bioz Verified Symbol Qiagen is a verified supplier
Bioz Manufacturer Symbol Qiagen manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 99
      Buy from Supplier

    Structured Review

    Qiagen rneasy mini kit
    Rneasy Mini Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 40568 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+transcriptome+array+(hta)+microarray+platform/RNeasy+Mini+Kit/pmc05122773-1071-12-15
    Average 99 stars, based on 40568 article reviews
    rneasy mini kit - by Bioz Stars, 2026-09
    99/100 stars

    Images

    Related Articles

    Cell Culture:

    Article Title: Extracellular biogenic nanoscale mitochondria reprogram the wound microenvironment via ROS scavenging independent of cellular uptake
    Article Snippet: .. Total RNA was extracted from cultured cells and peri-wound tissue samples using the RNeasy Mini Kit (Qiagen, Cat# 74014), following the manufacturer's protocol. .. Reverse transcription was performed using the PrimeScriptTM RT Master Mix (Takara, Cat# RR036A).

    Synthesized:

    Article Title: METTL1 Aggravates Periodontitis Progression by Inhibiting Osteoblast Differentiation via m7G modification of SEMA4D
    Article Snippet: These plasmids were transfected into HPDLSCs using Lipofectamine 3000 (Invitrogen, Carlsbad, CA, USA). .. Total RNA was extracted from HPDLSCs using the RNeasy Mini Kit (Qiagen, Hilden, Germany). cDNA was synthesized from 1 μg of total RNA using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Foster City, CA, USA). qPCR was performed using the PowerUp SYBR Green Master Mix (Applied Biosystems, Foster City, CA, USA) on the QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems, Foster City, CA, USA). ..

    Article Title: Spinal cord extracellular matrix hydrogel enhances organoid maturation and functional regeneration after spinal cord injury
    Article Snippet: The area of SCO cultured in each hydrogel condition was measured using bright-field images obtained through an optical microscopy (EVOSTM FL Auto Imaging System, Thermo Fisher Scientific) and analyzed with ImageJ software. .. The mRNA expression of SCO was quantified with qPCR analysis. mRNA samples were prepared using RNeasy Mini Kit (74106, QIAGEN GmbH, Hamburg, Germany), and cDNA samples were synthesized from the extracted mRNA using a cDNA Synthesis Kit (TakaRa). .. Subsequently, qPCR analysis was conducted using cDNA samples along with TaqMan Fast Universal PCR Master Mix (Applied Biosystems, Foster City, CA, USA) on a StepOnePlus Real-Time PCR System (Applied Biosystems).

    Reverse Transcription:

    Article Title: METTL1 Aggravates Periodontitis Progression by Inhibiting Osteoblast Differentiation via m7G modification of SEMA4D
    Article Snippet: These plasmids were transfected into HPDLSCs using Lipofectamine 3000 (Invitrogen, Carlsbad, CA, USA). .. Total RNA was extracted from HPDLSCs using the RNeasy Mini Kit (Qiagen, Hilden, Germany). cDNA was synthesized from 1 μg of total RNA using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Foster City, CA, USA). qPCR was performed using the PowerUp SYBR Green Master Mix (Applied Biosystems, Foster City, CA, USA) on the QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems, Foster City, CA, USA). ..

    Real-time Polymerase Chain Reaction:

    Article Title: METTL1 Aggravates Periodontitis Progression by Inhibiting Osteoblast Differentiation via m7G modification of SEMA4D
    Article Snippet: These plasmids were transfected into HPDLSCs using Lipofectamine 3000 (Invitrogen, Carlsbad, CA, USA). .. Total RNA was extracted from HPDLSCs using the RNeasy Mini Kit (Qiagen, Hilden, Germany). cDNA was synthesized from 1 μg of total RNA using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Foster City, CA, USA). qPCR was performed using the PowerUp SYBR Green Master Mix (Applied Biosystems, Foster City, CA, USA) on the QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems, Foster City, CA, USA). ..

    Article Title: Spinal cord extracellular matrix hydrogel enhances organoid maturation and functional regeneration after spinal cord injury
    Article Snippet: The area of SCO cultured in each hydrogel condition was measured using bright-field images obtained through an optical microscopy (EVOSTM FL Auto Imaging System, Thermo Fisher Scientific) and analyzed with ImageJ software. .. The mRNA expression of SCO was quantified with qPCR analysis. mRNA samples were prepared using RNeasy Mini Kit (74106, QIAGEN GmbH, Hamburg, Germany), and cDNA samples were synthesized from the extracted mRNA using a cDNA Synthesis Kit (TakaRa). .. Subsequently, qPCR analysis was conducted using cDNA samples along with TaqMan Fast Universal PCR Master Mix (Applied Biosystems, Foster City, CA, USA) on a StepOnePlus Real-Time PCR System (Applied Biosystems).

    SYBR Green Assay:

    Article Title: METTL1 Aggravates Periodontitis Progression by Inhibiting Osteoblast Differentiation via m7G modification of SEMA4D
    Article Snippet: These plasmids were transfected into HPDLSCs using Lipofectamine 3000 (Invitrogen, Carlsbad, CA, USA). .. Total RNA was extracted from HPDLSCs using the RNeasy Mini Kit (Qiagen, Hilden, Germany). cDNA was synthesized from 1 μg of total RNA using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Foster City, CA, USA). qPCR was performed using the PowerUp SYBR Green Master Mix (Applied Biosystems, Foster City, CA, USA) on the QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems, Foster City, CA, USA). ..

    Isolation:

    Article Title: Effects of dietary Chlorella vulgaris and cinnamon oil on growth performance, gut morphology, digestive enzymes, and intestinal gene expression in broilers
    Article Snippet: .. Total RNA was isolated from jejunal tissue samples using TRIzol® reagent (Invitrogen Life Technologies, Palo Alto, CA, USA) and subsequently purified with the RNeasy Mini Kit (Qiagen, Valencia, CA, USA). .. The integrity of RNA was verified by agarose gel electrophoresis, and concentrations were determined with a NanoDrop 1000 spectrophotometer (Thermo Scientific, Wilmington, DE, USA).

    Article Title: Obesity as a Determinant of Periodontal Therapy Outcomes: Insights on Oxidative and Endoplasmic Reticulum Stress Pathways
    Article Snippet: Membranes were incubated with mouse lgG secondary antibodies (Thermo Fisher Scientific Cat# 31430, RRID:AB_228307) and chemiluminescence was detected using ECL Plus reagent (Cell Signalling, Danvers, MA, USA), visualized with a Fusion FX5 system, and quantified by densitometry using Bio1D software ( https://www.vilber.com ,(RRID:SCR_023580). .. Total RNA was isolated from PBMCs using the RNeasy Mini Kit (Qiagen, Venlo, Netherlands), following the manufacturer’s protocol. .. Subsequently, 1 μg of RNA was reverse transcribed into cDNA using the RevertAid First Strand cDNA Synthesis Kit (Thermo Scientific, Waltham, MA, USA). qPCR was performed employing the FastStart Universal SYBR Green Master (Roche Applied Science, Penzberg, Germany) in a 7500 Fast Real-Time qPCR System (Life Technologies, Carlsbad, CA, USA).

    Article Title: Identification of small molecules that enhance aminoglycoside-mediated suppression of CFTR and NF1 nonsense mutations
    Article Snippet: .. Total RNA was isolated from cells using the Qiagen RNeasy Mini Kit (Qiagen, 74106) with optional QIAshredder (Qiagen, 79656). .. 1 μg of RNA was reverse transcribed using BioRad iScript reverse transcriptase (Bio-Rad, 1708841) in a Bio-Rad C1000 Touch Thermal Cycler.

    Purification:

    Article Title: Effects of dietary Chlorella vulgaris and cinnamon oil on growth performance, gut morphology, digestive enzymes, and intestinal gene expression in broilers
    Article Snippet: .. Total RNA was isolated from jejunal tissue samples using TRIzol® reagent (Invitrogen Life Technologies, Palo Alto, CA, USA) and subsequently purified with the RNeasy Mini Kit (Qiagen, Valencia, CA, USA). .. The integrity of RNA was verified by agarose gel electrophoresis, and concentrations were determined with a NanoDrop 1000 spectrophotometer (Thermo Scientific, Wilmington, DE, USA).

    RNA Extraction:

    Article Title: Development of a PTEN -siRNA activated scaffold to promote axonal regrowth following spinal cord injury
    Article Snippet: .. RNA extraction of cells was carried out using the RNeasy mini kit (Qiagen, Ireland) according to the manufacturer's protocol, and the extracted RNA was eluted into molecular grade H 2 O. RNA quantity and quality were then assessed using a NanoDrop (NeoDot – UV/VIS Nano Spectrophotometer; Neo Biotech). .. Between 200 ng and 400ng of RNA (ensuring uniform amounts between samples and within experiments) were reverse transcribed using a QuantiTect reverse transcription kit as per the manufacturer's instructions (Qiagen, Ireland).

    Spectrophotometry:

    Article Title: Development of a PTEN -siRNA activated scaffold to promote axonal regrowth following spinal cord injury
    Article Snippet: .. RNA extraction of cells was carried out using the RNeasy mini kit (Qiagen, Ireland) according to the manufacturer's protocol, and the extracted RNA was eluted into molecular grade H 2 O. RNA quantity and quality were then assessed using a NanoDrop (NeoDot – UV/VIS Nano Spectrophotometer; Neo Biotech). .. Between 200 ng and 400ng of RNA (ensuring uniform amounts between samples and within experiments) were reverse transcribed using a QuantiTect reverse transcription kit as per the manufacturer's instructions (Qiagen, Ireland).

    Expressing:

    Article Title: Spinal cord extracellular matrix hydrogel enhances organoid maturation and functional regeneration after spinal cord injury
    Article Snippet: The area of SCO cultured in each hydrogel condition was measured using bright-field images obtained through an optical microscopy (EVOSTM FL Auto Imaging System, Thermo Fisher Scientific) and analyzed with ImageJ software. .. The mRNA expression of SCO was quantified with qPCR analysis. mRNA samples were prepared using RNeasy Mini Kit (74106, QIAGEN GmbH, Hamburg, Germany), and cDNA samples were synthesized from the extracted mRNA using a cDNA Synthesis Kit (TakaRa). .. Subsequently, qPCR analysis was conducted using cDNA samples along with TaqMan Fast Universal PCR Master Mix (Applied Biosystems, Foster City, CA, USA) on a StepOnePlus Real-Time PCR System (Applied Biosystems).

    cDNA Synthesis:

    Article Title: Spinal cord extracellular matrix hydrogel enhances organoid maturation and functional regeneration after spinal cord injury
    Article Snippet: The area of SCO cultured in each hydrogel condition was measured using bright-field images obtained through an optical microscopy (EVOSTM FL Auto Imaging System, Thermo Fisher Scientific) and analyzed with ImageJ software. .. The mRNA expression of SCO was quantified with qPCR analysis. mRNA samples were prepared using RNeasy Mini Kit (74106, QIAGEN GmbH, Hamburg, Germany), and cDNA samples were synthesized from the extracted mRNA using a cDNA Synthesis Kit (TakaRa). .. Subsequently, qPCR analysis was conducted using cDNA samples along with TaqMan Fast Universal PCR Master Mix (Applied Biosystems, Foster City, CA, USA) on a StepOnePlus Real-Time PCR System (Applied Biosystems).



    Similar Products

    human transcriptome array (hta) microarray platform  (Thermo Fisher)
    90
    Thermo Fisher human transcriptome array (hta) microarray platform
    Individual heat-map and PCA analysis of five individual subjects show similar trends but potentially retained some degree of variability in temporal induction and magnitude between donors. (A) Individual heat-maps of different subjects revealed largely similar patterns with potential differences in temporal induction from 8 to 48 hpi, including the magnitude of induction. (B) Individual PCA plot showed that the <t>transcriptomes</t> across different subjects were not clustered as tightly since the hNEC samples were of different genetic backgrounds. The PCA plot showed a distinct progression of differential gene changes across time-points even at 0 hpi. (C) Individual viral titers increased over time to reach high viral loads in samples from all donors. The individual variation was deemed not clinically significant given that a sufficiently high virus titer was attained to induce a consistent response.
    Human Transcriptome Array (Hta) Microarray Platform, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+transcriptome+array+(hta)+microarray+platform/pmc06246735-48-13-12
    Average 90 stars, based on 1 article reviews
    human transcriptome array (hta) microarray platform - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Individual heat-map and PCA analysis of five individual subjects show similar trends but potentially retained some degree of variability in temporal induction and magnitude between donors. (A) Individual heat-maps of different subjects revealed largely similar patterns with potential differences in temporal induction from 8 to 48 hpi, including the magnitude of induction. (B) Individual PCA plot showed that the transcriptomes across different subjects were not clustered as tightly since the hNEC samples were of different genetic backgrounds. The PCA plot showed a distinct progression of differential gene changes across time-points even at 0 hpi. (C) Individual viral titers increased over time to reach high viral loads in samples from all donors. The individual variation was deemed not clinically significant given that a sufficiently high virus titer was attained to induce a consistent response.

    Journal: Frontiers in Microbiology

    Article Title: Comparative Transcriptomic and Metagenomic Analyses of Influenza Virus-Infected Nasal Epithelial Cells From Multiple Individuals Reveal Specific Nasal-Initiated Signatures

    doi: 10.3389/fmicb.2018.02685

    Figure Lengend Snippet: Individual heat-map and PCA analysis of five individual subjects show similar trends but potentially retained some degree of variability in temporal induction and magnitude between donors. (A) Individual heat-maps of different subjects revealed largely similar patterns with potential differences in temporal induction from 8 to 48 hpi, including the magnitude of induction. (B) Individual PCA plot showed that the transcriptomes across different subjects were not clustered as tightly since the hNEC samples were of different genetic backgrounds. The PCA plot showed a distinct progression of differential gene changes across time-points even at 0 hpi. (C) Individual viral titers increased over time to reach high viral loads in samples from all donors. The individual variation was deemed not clinically significant given that a sufficiently high virus titer was attained to induce a consistent response.

    Article Snippet: Gene microarrays for the infected and control hNEC samples were performed using Affymetrix human transcriptome array (HTA) microarray platform.

    Techniques: