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gapdh reference gene sequence  (OriGene)


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    OriGene gapdh reference gene sequence
    Gapdh Reference Gene Sequence, supplied by OriGene, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+reference+sequences/GAPDH+Human+qPCR+Template+Standard/pm40933471-98-1-8
    Average 93 stars, based on 5 article reviews
    gapdh reference gene sequence - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Gene Expression:

    Article Title: High CRLF2 expression associates with IKZF1 dysfunction in adult acute lymphoblastic leukemia without CRLF2 rearrangement.
    Article Snippet: Cytogenetic analysis, determination of Ik6 [23], CRLF2 rearrangements [3, 10, 21], BCR-ABL fusion gene/ Ph chromosome [8, 23] were performed as previously described. qPCR was performed with qSTAR SYBR Master Mix (OriGene) on StepOne Plus Real-time PCR system (Applied Bioscience). .. In order to quantitate gene expression value in patients’ samples, template standards and primers against Homo sapiens gene CRLF2 (OriGene, USA) and Homo sapiens housekeeping gene GAPDH (OriGene, USA) were obtained from OriGeneTechnologies (Rockville, MD, USA). ..

    Article Title: WDR5 high expression and its effect on tumorigenesis in leukemia.
    Article Snippet: .. In order to quantitate gene expression value in patients’ samples, template standards and primers against Homo sapiens gene WDR5 (HK204310, OriGene, USA) and Homo sapiens housekeeping gene GAPDH, HK203273, OriGene, USA) were obtained from OriGene Technologies (Rockville, MD). ..

    Sequencing:

    Article Title: GAPDH controls extracellular vesicle biogenesis and enhances the therapeutic potential of EV mediated siRNA delivery to the brain
    Article Snippet: .. The cDNA sequence of human GAPDH (OriGene, UK) was inserted into the pET-28b(+) vector (Novagen). ..

    Article Title: Identification of Diagnostic Biomarkers for Myocardial Infarction Using Bioinformatics and Disulfidptosis-Targeted Computational Drug Discovery.
    Article Snippet: .. The GAPDH reference gene sequence was obtained from OriGene (https://www.origene.com.cn/). ..

    Article Title: GAPDH controls extracellular vesicle biogenesis and enhances therapeutic potential of EVs in silencing the Huntingtin gene in mice via siRNA delivery
    Article Snippet: .. The cDNA sequence of human GAPDH (OriGene, UK) was inserted into the pET-28b(+) vector (Novagen). ..

    Plasmid Preparation:

    Article Title: GAPDH controls extracellular vesicle biogenesis and enhances the therapeutic potential of EV mediated siRNA delivery to the brain
    Article Snippet: .. The cDNA sequence of human GAPDH (OriGene, UK) was inserted into the pET-28b(+) vector (Novagen). ..

    Article Title: GAPDH controls extracellular vesicle biogenesis and enhances therapeutic potential of EVs in silencing the Huntingtin gene in mice via siRNA delivery
    Article Snippet: .. The cDNA sequence of human GAPDH (OriGene, UK) was inserted into the pET-28b(+) vector (Novagen). ..

    other:

    Article Title: TGF-β/SMAD3 Pathway Stimulates Sphingosine-1 Phosphate Receptor 3 Expression
    Article Snippet: Subsequently, sections were incubated with peroxidase substrate (ImmPACT DAB (3,3′-diaminobenzidine), Vector Laboratories, catalog number SK-4105) until the desired stain intensity develops.

    Article Title: ZCWPW1 is recruited to recombination hotspots by PRDM9 and is essential for meiotic double strand break repair
    Article Snippet: DOI: https://doi.org/10.7554/eLife.53392 21 of 36 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Anti-FLAG M2 (mouse monoclonal) Sigma-Aldrich Cat. F3165, RRID:AB_259529 IF (1:500), WB (1:2000), IP (3 mg) Antibody Anti-b-Actin (mouse monoclonal) Sigma-Aldrich Cat. A1978, RRID:AB_476692 WB (1:2000) Antibody ECL Rabbit IgG, HRP-linked whole Ab (donkey polyclonal) GE Healthcare Cat. NA934, RRID:AB_772206 WB (1:10000) Antibody ECL Mouse IgG, HRP-linked whole Ab (sheep polyclonal) GE Healthcare Cat. NA931, RRID:AB_772210 WB (1:10000) Sequence-based reagent pIRESMinor Chan et al., 2017 biotin labelled minor satellite probe Sequence-based reagent GAPDH_F (Human) OriGene PCR primers, transcript detection, NM_002046 GCTCCTCTGACTT CAACAGCGGCT Sequence-based reagent GAPDH_R (Human) OriGene PCR primers, transcript detection, NM_002046 ACCACCCTGTTG CTGTAGCCAA Sequence-based reagent PRDM9_F (Human) OriGene PCR primers, transcript detection, NM_020227 ACGAAGAGGCAG CCAACAATGG Sequence-based reagent PRDM9_R (Human) OriGene PCR primers, transcript detection, NM_020227 GCCACCAGGTT CTGCTCTTCAT Sequence-based reagent ZCWPW1_F (Human) OriGene PCR primers, transcript detection, NM_017984 GATGGCTCAAGA GGCAGAACAG Sequence-based reagent ZCWPW1_R (Human) OriGene PCR primers, transcript detection, NM_017984 TGGGCTGTTCAA ACCAGAGAGC Sequence-based reagent ZCWPW2_F (Human) OriGene PCR primers, transcript detection, NM_001040432 AAGAGCTGGAG CAAATGCTGCAG Sequence-based reagent ZCWPW2_R (Human) OriGene PCR primers, transcript detection, NM_001040432 CAGGAGCTTCTG GGCTGCATTT Commercial assay or kit Telomere PNA FISH Kit/Cy3 Agilent Cat. K5326 Commercial assay or kit Pierce BCA protein assay kit Thermo Fisher Scientific Cat. 23227 Commercial assay or kit ECL Prime Western Blotting Detection Reagent GE Healthcare Cat. 10308449 Commercial assay or kit Minelute Reaction Cleanup Kit QIAGEN Cat. 28204 Commercial assay or kit Qubit dsDNA HS Assay kit Thermo Fisher Scientific Cat. Q32851 Chemical compound, drug IPTG Sigma-Aldrich Cat. I5502 0.5 mM final Other Fast SYBR Green Master Mix Applied Biosystems Cat. 4385610 RNA extraction and RT-qPCR Other Dynabeads M-280 Sheep anti-Rabbit IgG Thermo Fisher Scientific Cat. 11203D, RRID:AB_2783009 IP and ChIP experiments; IP (25–75 ul), ChIP (65 ul) Other Dynabeads M-280 Sheep anti-Mouse IgG Thermo Fisher Scientific Cat. 11202D, RRID:AB_2783640 IP and ChIP experiments; IP (25 ul), ChIP (65 ul) Continued on next page Wells et al. eLife 2020;9:e53392.



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    Image Search Results


    Schematic overview of training and inference workflows. A) Training phase: Positive and negative sample pairs are processed through the embedding module to generate four modality-specific embedding representations, human/virus sequence embedding is encoded by ESM-2 to represent amino acid sequence features, human/virus chemical embedding is extracted from AAindex profiles to represent protein biochemical properties. The cross-fusion module computes loss via contrastive learning and executes forward propagation. Model parameters are iteratively updated using the Adam optimizer, with final weights preserved for inference. B) Binary task inference. Pre-trained weights are loaded to compute task-specific losses, enabling downstream classification prediction. C) Conditional generative inference. A sequence decoder module translates fused modality embeddings into human protein sequences, with outputs ranked to return the top five highest-confidence matches.

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    Article Title: DeepHVI: A multimodal deep learning framework for predicting human-virus protein-protein interactions using protein language models

    doi: 10.1016/j.bsheal.2025.07.005

    Figure Lengend Snippet: Schematic overview of training and inference workflows. A) Training phase: Positive and negative sample pairs are processed through the embedding module to generate four modality-specific embedding representations, human/virus sequence embedding is encoded by ESM-2 to represent amino acid sequence features, human/virus chemical embedding is extracted from AAindex profiles to represent protein biochemical properties. The cross-fusion module computes loss via contrastive learning and executes forward propagation. Model parameters are iteratively updated using the Adam optimizer, with final weights preserved for inference. B) Binary task inference. Pre-trained weights are loaded to compute task-specific losses, enabling downstream classification prediction. C) Conditional generative inference. A sequence decoder module translates fused modality embeddings into human protein sequences, with outputs ranked to return the top five highest-confidence matches.

    Article Snippet: Since model-generated sequences may not correspond to natural proteins, we retrieved 20,078 human reference protein sequences from National Center of Biotechnology Information (NCBI) (accession: GCF_000001405.40) and 17,451 reviewed viral protein sequences from UniProt Swiss-Pro (taxon ID 10239) to ensure biological relevance.

    Techniques: Virus, Sequencing

    Cosine similarity analysis of generated human protein sequences and viral protein sequences. A) Cosine similarity between generated and ground-truth human proteins, illustrating the distribution of cosine similarity values between human protein sequences. B) Cosine similarity between generated and ground-truth viral proteins, depicting the distribution for viral protein sequences, which exhibits slightly lower and more variable similarity scores. In both cases, the distributions are sharply peaked around 0.8, indicating a generally strong semantic alignment across samples. Human and viral protein sequences from the test set were analyzed using DeepHVI to generate a density distribution of similarity scores between reconstructed sequences and human interactors. Abbreviations: Std, standard deviation; Min, minimum; Max, maximum.

    Journal: Biosafety and Health

    Article Title: DeepHVI: A multimodal deep learning framework for predicting human-virus protein-protein interactions using protein language models

    doi: 10.1016/j.bsheal.2025.07.005

    Figure Lengend Snippet: Cosine similarity analysis of generated human protein sequences and viral protein sequences. A) Cosine similarity between generated and ground-truth human proteins, illustrating the distribution of cosine similarity values between human protein sequences. B) Cosine similarity between generated and ground-truth viral proteins, depicting the distribution for viral protein sequences, which exhibits slightly lower and more variable similarity scores. In both cases, the distributions are sharply peaked around 0.8, indicating a generally strong semantic alignment across samples. Human and viral protein sequences from the test set were analyzed using DeepHVI to generate a density distribution of similarity scores between reconstructed sequences and human interactors. Abbreviations: Std, standard deviation; Min, minimum; Max, maximum.

    Article Snippet: Since model-generated sequences may not correspond to natural proteins, we retrieved 20,078 human reference protein sequences from National Center of Biotechnology Information (NCBI) (accession: GCF_000001405.40) and 17,451 reviewed viral protein sequences from UniProt Swiss-Pro (taxon ID 10239) to ensure biological relevance.

    Techniques: Generated, Standard Deviation