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Arraystar inc mouse epitranscriptomic microarray

A m 6 A-RIP <t>microarray</t> analysis (upper) showing inhibitory effects of ALKBH5 on m 6 A methylation of pre-miRNAs relative to the control group. Two potentially m 6 A sites of pre-miR-181b-1 predicted by SRAMP program (lower). B m 6 A methylation modification of miR-181-5p detected by gene-specific m 6 A assay. C pre-miR-181b-1 and miR-181-5p endogenous levels in osteosarcoma cell lines compared with hOB cells. D qRT-PCR analysis revealed the function of ALKBH5 overexpression or knockdown on pre-miR-18b-1 and miR-181-5p expression. E Wound-healing assay performed at 0 and 24 h, respectively, after transfected with NC or miR-181-5p mimics. Bar graph representing mean relative distance of migrated cells (Bar: 200 μm, n = 4). F Representative images of EdU staining in U2OS cells with or without miR-181-5p mimics. Bar graph quantifying the percentage of EdU-positive cells (Bar: 25 μm, n = 5). G Migration ability of U2OS after transfected with ALKBH5 plasmids and/or co-transfected with miR-181-5p inhibitor (AMO-181-5p) (Bar: 200 μm, n = 4). H EdU staining showing the reversing effects of AMO-181-5p on cell proliferation (Bar: 25 μm, n = 5). Data are expressed as mean ± SEM. * P < 0.05; ** P < 0.01; *** P < 0.001 (vs. the first group). ### P < 0.001 (vs. the second group).

Mouse Epitranscriptomic Microarray, supplied by Arraystar inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+m6a+epitranscriptomic+microarray/mouse+rna+epi+transcriptomic+microarray/pmc07801648-353-8-12
Average 90 stars, based on 1 article reviews

mouse epitranscriptomic microarray - by Bioz Stars, 2026-10

90/100 stars

Images

1) Product Images from "ALKBH5 suppresses tumor progression via an m 6 A-dependent epigenetic silencing of pre-miR-181b-1/YAP signaling axis in osteosarcoma"

Article Title: ALKBH5 suppresses tumor progression via an m 6 A-dependent epigenetic silencing of pre-miR-181b-1/YAP signaling axis in osteosarcoma

Journal: Cell Death & Disease

doi: 10.1038/s41419-020-03315-x

A m 6 A-RIP microarray analysis (upper) showing inhibitory effects of ALKBH5 on m 6 A methylation of pre-miRNAs relative to the control group. Two potentially m 6 A sites of pre-miR-181b-1 predicted by SRAMP program (lower). B m 6 A methylation modification of miR-181-5p detected by gene-specific m 6 A assay. C pre-miR-181b-1 and miR-181-5p endogenous levels in osteosarcoma cell lines compared with hOB cells. D qRT-PCR analysis revealed the function of ALKBH5 overexpression or knockdown on pre-miR-18b-1 and miR-181-5p expression. E Wound-healing assay performed at 0 and 24 h, respectively, after transfected with NC or miR-181-5p mimics. Bar graph representing mean relative distance of migrated cells (Bar: 200 μm, n = 4). F Representative images of EdU staining in U2OS cells with or without miR-181-5p mimics. Bar graph quantifying the percentage of EdU-positive cells (Bar: 25 μm, n = 5). G Migration ability of U2OS after transfected with ALKBH5 plasmids and/or co-transfected with miR-181-5p inhibitor (AMO-181-5p) (Bar: 200 μm, n = 4). H EdU staining showing the reversing effects of AMO-181-5p on cell proliferation (Bar: 25 μm, n = 5). Data are expressed as mean ± SEM. * P < 0.05; ** P < 0.01; *** P < 0.001 (vs. the first group). ### P < 0.001 (vs. the second group).

Figure Legend Snippet: A m 6 A-RIP microarray analysis (upper) showing inhibitory effects of ALKBH5 on m 6 A methylation of pre-miRNAs relative to the control group. Two potentially m 6 A sites of pre-miR-181b-1 predicted by SRAMP program (lower). B m 6 A methylation modification of miR-181-5p detected by gene-specific m 6 A assay. C pre-miR-181b-1 and miR-181-5p endogenous levels in osteosarcoma cell lines compared with hOB cells. D qRT-PCR analysis revealed the function of ALKBH5 overexpression or knockdown on pre-miR-18b-1 and miR-181-5p expression. E Wound-healing assay performed at 0 and 24 h, respectively, after transfected with NC or miR-181-5p mimics. Bar graph representing mean relative distance of migrated cells (Bar: 200 μm, n = 4). F Representative images of EdU staining in U2OS cells with or without miR-181-5p mimics. Bar graph quantifying the percentage of EdU-positive cells (Bar: 25 μm, n = 5). G Migration ability of U2OS after transfected with ALKBH5 plasmids and/or co-transfected with miR-181-5p inhibitor (AMO-181-5p) (Bar: 200 μm, n = 4). H EdU staining showing the reversing effects of AMO-181-5p on cell proliferation (Bar: 25 μm, n = 5). Data are expressed as mean ± SEM. * P < 0.05; ** P < 0.01; *** P < 0.001 (vs. the first group). ### P < 0.001 (vs. the second group).

Techniques Used: Microarray, Methylation, Modification, Quantitative RT-PCR, Over Expression, Expressing, Wound Healing Assay, Transfection, Staining, Migration

other:

Article Title: Epitranscriptomic Analysis of the Ventral Hippocampus in a Mouse Model of Post-Traumatic Stress Disorder Following Deep Brain Stimulation Treatment of the Basolateral Amygdala.
Article Snippet: The amalgamated labeled cRNAs were then hybridized onto the Arraystar Mouse mRNA & lncRNA Epitranscriptomic Microarray (8 × 60K, Arraystar, Rockville, MD, USA).

Article Title: METTL3-m6A methylation inhibits the proliferation and viability of type II alveolar epithelial cells in acute lung injury by enhancing the stability and translation efficiency of Pten mRNA.
Article Snippet: These cRNAs were subsequently combined and hybridized onto the Arraystar Mouse mRNA&lncRNA Epitranscriptomic Microarray (8 × 60 K, Arraystar).

Article Title: METTL16 promotes corneal nerve regeneration in diabetic corneal neuropathy.
Article Snippet: As a leading ocular disorder caused by diabetes mellitus, diabetic corneal neuropathy (DCN) is characterized by decreased corneal sensation, ocular irritation, delayed corneal wound healing and corneal neuropathic pain.. Several researchers including us previously evidenced that DCN is regulated by several transcriptional and/or epigenetic factors.. However, the role of N6-methyladenosine (m6A) modification, the most common internal modification in eukaryotic RNAs, in the pathogenesis of DCN remains largely unexplored.

Labeling:

Article Title: Microarray analysis of tRNA-derived small RNA (tsRNA) in LPS-challenged macrophages treated with metformin.
Article Snippet: Metformin, a widely used anti-diabetic drug, has demonstrated its efficacy in addressing various inflammatory conditions. tRNA-derived small RNA (tsRNA), a novel type of small non-coding RNA, exhibits diverse regulatory functions and holds promise as both a diagnostic biomarker and a therapeutic target for various diseases.. The purpose of this study is to investigate whether the abundance of tsRNAs changed in LPS versus LPS + metformintreated cells, utilizing microarray technology.. Firstly, we established an in vitro lipopolysaccharide (LPS)induced inflammation model using RAW264.7 macrophages and assessed the protective effects of metformin against inflammatory damage.

Microarray:

Article Title: METTL3-mediated pre-miR-665/DLX3 m 6 A methylation facilitates the committed differentiation of stem cells from apical papilla
Article Snippet: An Arraystar Super RNA Labeling Kit was used to designate these RNAs as cRNAs on their own. .. The cRNAs were combined and hybridized onto the Arraystar Mouse Epitranscriptomic Microarray (8 × 60 K, Arraystar). .. An Agilent G2505C scanner was used to scan the arrays in two-color channels after washing the slides.

Article Title: The m7G Methyltransferase Mettl1 Drives Cardiac Hypertrophy by Regulating SRSF9-Mediated Splicing of NFATc4.
Article Snippet: The “IP” and “Sup” RNAs were labeled with Cy5 and Cy3 respectively as cRNAs in separate reactions using Arraystar RNA Labeling protocol. .. The cRNAs were combined and hybridized onto Arraystar Mouse mRNA Epitranscriptomic Microarray (8 × 60 K, Arraystar). .. After washing the slides, the arrays were scanned in two-color channels by an Agilent Scanner G2505C.

Article Title: The m7G Methyltransferase Mettl1 Drives Cardiac Hypertrophy by Regulating SRSF9‐Mediated Splicing of NFATc4
Article Snippet: The “IP” and “Sup” RNAs were labeled with Cy5 and Cy3 respectively as cRNAs in separate reactions using Arraystar RNA Labeling protocol. .. The cRNAs were combined and hybridized onto Arraystar Mouse mRNA Epitranscriptomic Microarray (8 × 60 K, Arraystar). .. After washing the slides, the arrays were scanned in two‐color channels by an Agilent Scanner G2505C.