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Arraystar inc human circrna epitranscriptomic microarray
Human Circrna Epitranscriptomic Microarray, supplied by Arraystar inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+m6a+epitranscriptomic+microarray/human+circrna+epitranscriptomic+microarray/pmc11408563-58-9-17
Average 90 stars, based on 1 article reviews
human circrna epitranscriptomic microarray - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

Labeling:

Article Title: Circular RNA circDhx32 promotes cardiac inflammatory responses in mouse cardiac ischemia-reperfusion injury via binding to FOXO1 competed with AdipoR1.
Article Snippet: Next, the enriched “IP” and “Sup” RNAs were labeled with Cy3 (for “Sup”) or Cy5 (for “IP”) and purified using a Super RNA Labeling Kit (Arraystar, MD, USA) and a RNeasy Mini Kit, respectively. .. The labeled RNAs were subsequently hybridized onto an Arraystar circRNA Epitranscriptomic Microarray (8 × 15 K, Arraystar). .. After being scanned in two color channels by an Agilent Scanner G2505C (Agilent, Beijing, China), the microarrays were analysed using Agilent Feature Extraction software (version 11.0.1.1).

Article Title: Exploring the Roles of m6A-Modified circRNAs in Myasthenia Gravis Based on Multi-Omics Analysis.
Article Snippet: Myasthenia gravis (MG) is an autoimmune disease mediated by autoantibodies.. The important roles of circRNAs modified by m6A methylation have been reported in the pathogenesis of other autoimmune diseases, but remain unclear in MG. To address this point, we collected peripheral blood mononuclear cells from six MG patients and six healthy controls and performed m6A‐circRNA epitranscriptomic microarray and RNA sequencing.. Differentially m6A‐modified circRNAs and differentially expressed genes (DEGs) were analyzed.

Microarray:

Article Title: Circular RNA circDhx32 promotes cardiac inflammatory responses in mouse cardiac ischemia-reperfusion injury via binding to FOXO1 competed with AdipoR1.
Article Snippet: Next, the enriched “IP” and “Sup” RNAs were labeled with Cy3 (for “Sup”) or Cy5 (for “IP”) and purified using a Super RNA Labeling Kit (Arraystar, MD, USA) and a RNeasy Mini Kit, respectively. .. The labeled RNAs were subsequently hybridized onto an Arraystar circRNA Epitranscriptomic Microarray (8 × 15 K, Arraystar). .. After being scanned in two color channels by an Agilent Scanner G2505C (Agilent, Beijing, China), the microarrays were analysed using Agilent Feature Extraction software (version 11.0.1.1).

Article Title: Exploring the Roles of m6A-Modified circRNAs in Myasthenia Gravis Based on Multi-Omics Analysis.
Article Snippet: Myasthenia gravis (MG) is an autoimmune disease mediated by autoantibodies.. The important roles of circRNAs modified by m6A methylation have been reported in the pathogenesis of other autoimmune diseases, but remain unclear in MG. To address this point, we collected peripheral blood mononuclear cells from six MG patients and six healthy controls and performed m6A‐circRNA epitranscriptomic microarray and RNA sequencing.. Differentially m6A‐modified circRNAs and differentially expressed genes (DEGs) were analyzed.

Article Title: High-throughput microarray reveals the epitranscriptome-wide landscape of m 6 A-modified circRNA in oral squamous cell carcinoma.
Article Snippet: Then, ‘Sup’ and ‘IP’ RNAs were administrated with RNase R (Epicentre, Inc.), and subsequently labeled with Cy3 and Cy5 respectively as cRNAs s in separate reactions by Arraystar Super RNA Labeling Kit (Arraystar, AL-SE-005). .. The cRNAs were combined and hybridized on Arraystar Human circRNA Epitranscriptomic Microarray (8 × 15 K, Arraystar). .. Slides were washed and the arrays were scanned by an Agilent Scanner G2505C in two-color channels.

Article Title: TRMT10C-mediated m7G modification of circFAM126A inhibits lung cancer growth by regulating cellular glycolysis
Article Snippet: In accordance with the principle of Arraystar RNA labelling, RNA was treated with RNase R and labelled as complementary RNA (cRNA) with Cy5 and Cy3. .. The cRNAs were combined and hybridized to the Arraystar Human circRNA Epitranscriptomic Microarray (8 × 15 K, Arraystar) (GSE232281). .. The circFAM126A, HSP90, and AKT1 overexpression plasmids and the corresponding control empty vectors (Fenghui Biotechnology, Changsha, China) were constructed, and the plasmid sequences were validated by sequencing.

Article Title: High-throughput microarray reveals the epitranscriptome-wide landscape of m 6 A-modified circRNA in oral squamous cell carcinoma
Article Snippet: Then, ‘Sup’ and ‘IP’ RNAs were administrated with RNase R (Epicentre, Inc.), and subsequently labeled with Cy3 and Cy5 respectively as cRNAs s in separate reactions by Arraystar Super RNA Labeling Kit (Arraystar, AL-SE-005). .. The cRNAs were combined and hybridized on Arraystar Human circRNA Epitranscriptomic Microarray (8 × 15 K, Arraystar). .. Slides were washed and the arrays were scanned by an Agilent Scanner G2505C in two-color channels.

Article Title: TRMT10C-mediated m7G modification of circFAM126A inhibits lung cancer growth by regulating cellular glycolysis.
Article Snippet: In accordance with the principle of Arraystar RNA labelling, RNA was treated with RNase R and labelled as complementary RNA (cRNA) with Cy5 and Cy3. .. The cRNAs were combined and hybridized to the Arraystar Human circRNA Epitranscriptomic Microarray (8 × 15 K, Arraystar) (GSE232281). .. Plasmid construction and transfection The circFAM126A, HSP90, and AKT1 overexpression plasmids and the corresponding control empty vectors (Fenghui Biotechnology, Changsha, China) were constructed, and the plasmid sequences were validated by sequencing.

other:

Article Title: The emerging functions and clinical implications of circRNAs in acute myeloid leukaemia
Article Snippet: – , Healthy control (n = 3) and AML (n = 4) , Arraystar Human CircRNA Epitran-scriptomic Microarray , 1136 m 6 A modified circRNAs were differentially expressed in the two groups, including 1057 up-regulated and 79 downregulated , [ ] .

Article Title: N6-methyladenosine hypomethylation of circGPATCH2L regulates DNA damage and apoptosis through TRIM28 in intervertebral disc degeneration.
Article Snippet: Circular RNAs (circRNAs) are a class of noncoding RNAs that have been found to be involved in intervertebral disc degeneration (IVDD) progression, and N6-methyladenosine (m6A) broadly exists in circRNAs.. Here, we identified circGPATCH2L with a low m6A methylation level to be upregulated in degenerative nucleus pulposus tissues.. Mechanistically, as a protein decoy for tripartite motif containing 28 (TRIM28) within aa 402–452 region, circGPATCH2L abrogates the phosphorylation of TRIM28 and inhibits P53 degradation, which contributes to DNA damage accumulation and cellular apoptosis and leads to IVDD progression.



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Image Search Results


Primer sequences for key  LncRNA  and GAPDH.

Journal: International Journal of Genomics

Article Title: M6A Modification and Transcription Analysis of LncRNA in Cerebral Ischemia/Reperfusion Injury

doi: 10.1155/2024/4596974

Figure Lengend Snippet: Primer sequences for key LncRNA and GAPDH.

Article Snippet: On the one hand, the m6A modification level of LncRNA in samples was detected with Arraystar human m6A-mRNA and lncRNA epitranscriptomic microarray (Arraystar) according to the instruction manual [ ].

Techniques: Sequencing

Network of LncRNA-miRNA-mRNA was constructed to demonstrate possible regulatory relationships among lncRNAs, miRNAs, and mRNAs (the top 10 most significant target genes). Red represents LncRNAs, blue represents miRNAs, and green represents mRNAs.

Journal: International Journal of Genomics

Article Title: M6A Modification and Transcription Analysis of LncRNA in Cerebral Ischemia/Reperfusion Injury

doi: 10.1155/2024/4596974

Figure Lengend Snippet: Network of LncRNA-miRNA-mRNA was constructed to demonstrate possible regulatory relationships among lncRNAs, miRNAs, and mRNAs (the top 10 most significant target genes). Red represents LncRNAs, blue represents miRNAs, and green represents mRNAs.

Article Snippet: On the one hand, the m6A modification level of LncRNA in samples was detected with Arraystar human m6A-mRNA and lncRNA epitranscriptomic microarray (Arraystar) according to the instruction manual [ ].

Techniques: Construct

The expression levels of the four key LncRNAs in the 12 pairs of blood were quantified using qRT-PCR. Comparing with the control group, the expression levels of LncRNA FAR2, LncRNA LINC02431, and LncRNA AL357060.1 were downregulated, while the expression level of LncRNA FOXD2-AS1 was upregulated. ∗∗∗∗ p < 0.0001.

Journal: International Journal of Genomics

Article Title: M6A Modification and Transcription Analysis of LncRNA in Cerebral Ischemia/Reperfusion Injury

doi: 10.1155/2024/4596974

Figure Lengend Snippet: The expression levels of the four key LncRNAs in the 12 pairs of blood were quantified using qRT-PCR. Comparing with the control group, the expression levels of LncRNA FAR2, LncRNA LINC02431, and LncRNA AL357060.1 were downregulated, while the expression level of LncRNA FOXD2-AS1 was upregulated. ∗∗∗∗ p < 0.0001.

Article Snippet: On the one hand, the m6A modification level of LncRNA in samples was detected with Arraystar human m6A-mRNA and lncRNA epitranscriptomic microarray (Arraystar) according to the instruction manual [ ].

Techniques: Expressing, Quantitative RT-PCR, Control

The methylation levels of the four key LncRNAs in the 12 pairs of blood were quantified using MeRip-qPCR. Comparing with control group, the methylation levels of LncRNA FAR2, LncRNA FOXD2-AS1, and LncRNA AL357060.1 were hypomethylated, while the methylation level of LncRNA LINC02431 was not significantly different. ∗∗∗∗ p < 0.0001. ns: nonsignificant.

Journal: International Journal of Genomics

Article Title: M6A Modification and Transcription Analysis of LncRNA in Cerebral Ischemia/Reperfusion Injury

doi: 10.1155/2024/4596974

Figure Lengend Snippet: The methylation levels of the four key LncRNAs in the 12 pairs of blood were quantified using MeRip-qPCR. Comparing with control group, the methylation levels of LncRNA FAR2, LncRNA FOXD2-AS1, and LncRNA AL357060.1 were hypomethylated, while the methylation level of LncRNA LINC02431 was not significantly different. ∗∗∗∗ p < 0.0001. ns: nonsignificant.

Article Snippet: On the one hand, the m6A modification level of LncRNA in samples was detected with Arraystar human m6A-mRNA and lncRNA epitranscriptomic microarray (Arraystar) according to the instruction manual [ ].

Techniques: Methylation, Control