enzyme linked immunosorbent assay elisa kits  (Biorbyt)

Journal: Bioorganic & medicinal chemistry

Article Title: α-Methylation Enhances the Potency of Isoprenoid Triazole Bisphosphonates as Geranylgeranyl Diphosphate Synthase Inhibitors

doi: 10.1016/j.bmc.2017.10.023

Figure Lengend Snippet: RPMI-8226 cells were incubated for 48 hours in the presence or absence of lovastatin (Lov, 10 μM) or varying concentrations of the test compounds. A) Intracellular lambda light chain concentrations were determined via ELISA. Data are expressed as a percentage of control (mean + SD, n=3). The * denotes p < 0.05 per unpaired two-tailed t-test. B) Immunoblot analysis of Rap1a (antibody detects only unmodified protein) and β-tubulin (as a loading control).

Article Snippet: 5.23 Lambda light chain ELISA Human lambda light chain kit (Bethyl Laboratories, Montgomery, TX) was used to quantify intracellular monoclonal protein levels of whole cell lysate.

Techniques: Incubation, Enzyme-linked Immunosorbent Assay, Control, Two Tailed Test, Western Blot

Journal: Bioorganic & medicinal chemistry

Article Title: α-Methylation Enhances the Potency of Isoprenoid Triazole Bisphosphonates as Geranylgeranyl Diphosphate Synthase Inhibitors

doi: 10.1016/j.bmc.2017.10.023

Figure Lengend Snippet: RPMI-8226 cells were incubated for 48 hours in the presence or absence of lovastatin (Lov, 10 μM) or varying concentrations of the test compound. A) Intracellular lambda light chain concentrations were determined via ELISA. Data are expressed as a percentage of control (mean + SD, n=3). The * denotes p < 0.05 per unpaired two-tailed t-test. B) Immunoblot analysis of Rap1a (antibody detects only unmodified protein) and β-tubulin (as a loading control).

Article Snippet: 5.23 Lambda light chain ELISA Human lambda light chain kit (Bethyl Laboratories, Montgomery, TX) was used to quantify intracellular monoclonal protein levels of whole cell lysate.

Techniques: Incubation, Enzyme-linked Immunosorbent Assay, Control, Two Tailed Test, Western Blot

Journal: Bioorganic & medicinal chemistry

Article Title: α-Methylation Enhances the Potency of Isoprenoid Triazole Bisphosphonates as Geranylgeranyl Diphosphate Synthase Inhibitors

doi: 10.1016/j.bmc.2017.10.023

Figure Lengend Snippet: RPMI-8226 cells were incubated for 48 hours in the presence or absence of lovastatin (Lov, 10 μM) or varying concentrations of the test compounds. A) Intracellular lambda light chain concentrations were determined via ELISA. Data are expressed as a percentage of control (mean + SD, n=3). The * denotes p < 0.05 per unpaired two-tailed t-test. B) Immunoblot analysis of Rap1a (antibody detects only unmodified protein) and β-tubulin (as a loading control).

Article Snippet: 5.23 Lambda light chain ELISA Human lambda light chain kit (Bethyl Laboratories, Montgomery, TX) was used to quantify intracellular monoclonal protein levels of whole cell lysate.

Techniques: Incubation, Enzyme-linked Immunosorbent Assay, Control, Two Tailed Test, Western Blot

Journal: Bioorganic & medicinal chemistry

Article Title: α-Methylation Enhances the Potency of Isoprenoid Triazole Bisphosphonates as Geranylgeranyl Diphosphate Synthase Inhibitors

doi: 10.1016/j.bmc.2017.10.023

Figure Lengend Snippet: RPMI-8226 cells were incubated for 48 hours in the presence or absence of lovastatin (Lov, 10 μM) or varying concentrations of the test compounds. A) Intracellular lambda light chain concentrations were determined via ELISA. Data are expressed as a percentage of control (mean + SD, n=3). The * denotes p < 0.05 per unpaired two-tailed t-test. B) Immunoblot analysis of Rap1a (antibody detects only unmodified protein) and β-tubulin (as a loading control).

Article Snippet: 5.23 Lambda light chain ELISA Human lambda light chain kit (Bethyl Laboratories, Montgomery, TX) was used to quantify intracellular monoclonal protein levels of whole cell lysate.

Techniques: Incubation, Enzyme-linked Immunosorbent Assay, Control, Two Tailed Test, Western Blot