gse14975 homo sapiens affymetrix human genome u133 plus 2 0 left atrial appendage (Thermo Fisher)

Thermo Fisher is a verified supplier

Thermo Fisher manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Thermo Fisher gse14975 homo sapiens affymetrix human genome u133 plus 2 0 left atrial appendage
Gse14975 Homo Sapiens Affymetrix Human Genome U133 Plus 2 0 Left Atrial Appendage, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+genome+u133a+array+microarray+data/D+ARABINOSE+100GR+100GR/ppr0236799-83-79-82
Average 94 stars, based on 1 article reviews

gse14975 homo sapiens affymetrix human genome u133 plus 2 0 left atrial appendage - by Bioz Stars, 2026-11

94/100 stars

Images

other:

Article Title: An omic and multidimensional spatial atlas from serial biopsies of an evolving metastatic breast cancer.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Helios NanoLab G3 DualBeam FIB-SEM FEI Company (now Thermo Fisher Scientific) https://www.thermofisher.com/ us/en/home/electronmicroscopy/products/ dualbeam-fib-sem-microscopes.html Gemini 550 Crossbeam FIB-SEM ZEISS International https://www.zeiss.com/ microscopy/us/products/ fib-sem-instruments/crossbeam.html Ultramicrotome Leica EM UC7 Leica Microsystems https://www.leica- microsystems.com/products/ sample-preparation-forelectron-microscopy/p/leica-em-uc7/ Trim90 diamond knife DiATOME https://www.diatomeknives.com/ product.aspx?pid=416 EM ACE600 High Vacuum Sputter Coater Leica Microsystems https://www.leica- microsystems.com/products/ sample-preparation-forelectron-microscopy/p/l eica-em-ace600/ Managed workforce (800 hours) CloudFactory https://www.cloudfactory.com/

Single Particle:

Article Title: Cryo-EM structure of the human alpha5beta3 GABAA receptor in complex with GABA and Nb25
Article Snippet: .. Property Value Source EM reconstruction method SINGLE PARTICLE Depositor Imposed symmetry POINT, C1 Depositor Number of particles used 161455 Depositor Resolution determination method FSC 0.143 CUT-OFF Depositor CTF correction method Not provided Microscope FEI TITAN KRIOS Depositor Voltage (kV) 300 Depositor Electron dose (e−/Å2) 56.0 Depositor Minimum defocus (nm) Not provided Maximum defocus (nm) Not provided Magnification Not provided Image detector GATAN K2 SUMMIT (4k x 4k) Depositor Maximum map value 22.949 Depositor Minimum map value -12.891 Depositor Average map value -0.023 Depositor Map value standard deviation 0.837 Depositor Recommended contour level 7 Depositor Map size (Å) 273.92, 273.92, 273.92 wwPDB Map dimensions 256, 256, 256 wwPDB Map angles (◦) 90.0, 90.0, 90.0 wwPDB Pixel spacing (Å) 1.07, 1.07, 1.07 Depositor ..

Microscopy:

Article Title: Meibomian gland lipid alterations and ocular surface sequela in Awat2 knockout murine model of meibomian gland dysfunction and evaporative dry eye disease.
Article Snippet: .. Eyelids (Awat2 KO n=5, WT n=6, 1- to 18-months-old to 142 investigate a spectrum of ages) were carefully removed, submerged in RNAlater and stored at -143 20C until total RNA was extracted using the GeneJET RNA Purification Kit (ThermoFisher 144 Scientific) as described previously.27 The corneal epithelium (Awat2 KO n=5, WT n=6, 3- to 16-145 months-old to investigate differing degrees of chronicity) was debrided with a #15 scalpel blade 146 using a surgical microscope (OPMI-6-SFC Universal S3 ENT Microscope, Zeiss, Jena, 147 Germany) and RNA was isolated using the GeneJET RNA Purification Kit (ThermoFisher 148 Scientific). .. RNA yield was quantified using a Nanodrop spectrophotometer and treated with 149 dsDNase (ThermoFisher Scientific) to remove residual genomic DNA.

Standard Deviation:

Immunohistochemistry:

Article Title: 20-hydroxyecdysone reprograms amino acid metabolism to support the metamorphic development of Helicoverpa armigera.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-LC3 antibody Abcam company Cat# ab109364; RRID:AB_10861928 Rabbit monoclonal anti-b-Actin antibody ABclonal Technology Cat# AC037; RRID:AB_2768235 Rabbit polyclonal anti-ASS antibody Boster Cat# A02212-1 Rabbit polyclonal anti-ARG antibody Servicebio Cat# GB11285 Phospho-histone H3 (Ser10) antibody Cell Signaling Technology Cat# 9701; RRID:AB_331535 Histone H3 antibody Proteintech Cat# 17168-1-AP; RRID:AB_2716755 Critical commercial assays Metabolome assay Suzhou BioNovoGene Metabolomics Platform N/A T7 RNAi Transcription Kit Vazyme Cat# TR102 FAAs determination Qingdao Kechuang Quality Testing Company N/A a-KG determination Qingdao Kechuang Quality Testing Company Cat# KT90094-B Tissue histology and immunohistochemistry Servicebio N/A Arginine MedChemExpress Cat# 74-79-3 a-KG MedChemExpress Cat# 328-50-7 Glu MedChemExpress Cat# 56-86-0 YF 488 Tunel cell apoptosis kit US EVERBRIGHT INC Cat# T6013S SuperView 488 caspase-3 assay kit US EVERBRIGHT INC Cat# S6007S p-nitro-blue tetrazolium chloride Sangon Biotech Cat# 298-83-9 5-bromo-4-chloro-3-indolyl phosphate Sangon Biotech Cat# 6578-06-9 Trizol TransGen Biotech Cat# ET101-01 Reverse transcription kit Abcam Cat# G492 TransStart Tip Green qPCR Supermix Aidlab Cat# pc3301 20E Cayman Chemical Cat# 16145 Cell culture medium Gibco Cat# 11300-043 Fetal bovine serum Gibco Cat# 16140063 CCK-8 US EVERBRIGHT INC Cat# C6005M Deposited data Raw data and corresponding original data in Figures This paper https://doi.org/10.57760/sciencedb.08424 Western Blot original figures This paper https://doi.org/10.57760/sciencedb.08424 Experimental models: Cell lines H. armigera epidermal cell line This paper N/A Experimental models: Organisms/strains H. armigera Henan Agricultural University N/A Oligonucleotides Primers are listed in Table S1 This paper N/A Software and algorithms Excel This paper N/A GraphPad Prism 7 This paper https://www.graphpad.com ImageJ This paper https://imagej.nih.gov/ij (Continued on next page) 16 Cell Reports 42, 112644, June 27, 2023 ..

TUNEL Assay:

Caspase-3 Assay:

Reverse Transcription:

Real-time Polymerase Chain Reaction:

Cell Culture:

CCK-8 Assay:

Western Blot:

Software:

Purification:

Isolation:

Recombinant:

Article Title: Obesity-associated hyperleptinemia alters the gliovascular interface of the hypothalamus to promote hypertension.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rat anti-ACSA-2 Milteny 130-097-678 Sheep anti-albumin Abcam Cat# ab8940; RRID: AB_306875 Mouse anti-claudin-5 Life technology Cat# 35-2500; RRID: AB_2533200 Goat anti-collagen IV Chemicon/Merck Cat# AB769; RRID: AB_11210995 Rabbit anti-phospho-p44/42 MAPK/ERK1/ 2) (Thr202/Tyr204) Cell Signaling Technology Cat# 9101; RRID: AB_331646 Goat anti-GFAP Dako Cat# Z0334; RRID: AB_10013382 Chicken anti-GFP Abcam Cat# ab13970; RRID: AB_300798 Rabbit anti-HIF-1a Abcam Cat# ab82832; RRID: AB_1860665 Goat anti-Iba1 Synaptic Systems Cat# 234 003; RRID: AB_10641962 Anti-Ki67 Abcam Cat# ab16667; RRID: AB_302459 Rabbit anti-laminin Dako Cat# Z0097; RRID: AB_2313665 Mouse anti-NeuN Merck Cat# MAB377; RRID: AB_2298772 Rabbit anti-phospho-STAT3 (Tyr705) (D3A7) Cell Signaling Technology Cat# 9145; RRID: AB_2491009 Rabbit anti-VEGF (A-20) Santa Cruz Cat# sc-152; RRID: AB_2212984 Mouse anti-b-actin (AC-15) (HRP) Abcam Cat# ab49900; RRID: AB_867494 Alexa Fluor 488 goat anti-chicken IgG Invitrogen Cat# A-11039; RRID: AB_142924 Alexa Fluor 647 donkey anti-goat IgG Invitrogen Cat# A-21447; RRID: AB_141844 Alexa Fluor 405 goat anti-mouse IgG Invitrogen Cat# A-31553; RRID: AB_221604 Alexa Fluor 568 donkey anti-rabbit IgG Invitrogen Cat# A10042; RRID: AB_2534017 Alexa Fluor 647 donkey anti-rabbit IgG Invitrogen Cat# A-31573; RRID: AB_2536183 Alexa Fluor 647 donkey anti-sheep IgG Invitrogen Cat# A21448; RRID: AB_1500712 Chemicals, peptides, and recombinant proteins Acetylcholine Sigma-Aldrich A6625 Benzyl alcohol Sigma-Aldrich 24122-1L Benzyl benzoate Sigma-Aldrich B6630-250ML Cobalt (II) chloride hexahydrate Sigma-Aldrich C8661-25G Dichloromethane Sigma-Aldrich 270997-100ML Doxycycline hyclate Sigma-Aldrich D9891 Durcupan Electron Microscopy Sciences 14040 Formvar Electron Microscopy Sciences 15800 Gelatin from porcine skin (SUMI) VWR International SAFSG1890 Glutaraldehyde Electron Microscopy Sciences 16316 Heparin Sigma-Aldrich H0200000 Isoproterenol Sigma-Aldrich I-5627 KCl Sigma-Aldrich P9541 MTBE Sigma-Aldrich 1634-04-4 NuPAGE LDS sample buffer (4x) Novex/Life Technologies NP008 Osmium Tetroxide Electron Microscopy Sciences 19150 Paraformaldehyde Carl Roth 0335.2 Pepsin DAKO/Agilent S3002 Primers Thermo Fisher N/A Recombinant mouse leptin R&D systems 498-OB (Continued on next page) Cell Metabolism 33, 1155–1170.e1–e10, June 1, 2021 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant Mouse VEGF164 R&D systems 493-MV Skim milk powder Sigma-Aldrich 70166-500 g Sodium nitroprusside Sigma-Aldrich 1614501 Tamoxifen Sigma-Aldrich T5648-1g TaqMan Gene Expression Assay Thermo Fisher N/A TaqMan Universal Master Mix II, no UNG Thermo Fisher 4440040 Tetrahydrofuran Sigma-Aldrich 186562-1L Thromboxane A2 U46619 Sigma-Aldrich CAS 56985-40-1 Triton X-100 Roche Diagnostics 11858620 Tween-20 Sigma-Aldrich 9005-64-5 Critical commercial assays Adult Brain Dissociation Milteny 130-107-677 Bright-Glo Luciferase Assay System Promega E2610 Mouse/Rat Leptin ELISA Alpco 22-LEPMS-E01 Pierce BCA protein assay Thermo Fisher 23228 QuickDetect catecholamine (mouse) ELISA Kit Enzo Lifesciences E4462 RNeasy Micro Kit QUIAGEN 74004 RNeasy Mini Kit QUIAGEN 74104 SMARTer PCR cDNA synthesis kit TaKaRa 634455 Experimental models: Organisms/strains Mouse Lepob/ob Jackson Laboratory B6.Cg-Lepob/J;BKS.Cg-Dock7m+/+ Mouse Lepdb/db Jackson Laboratory Leprdb/J; Maine, USA Mouse TRE-LeprtTA Dr. LeDuc; Columbia University; USA (Skowronski et al., 2020) N/A Mouse LepRb:Ai14 This paper B6.129(Cg)-LepRtm(cre)Rck/J;B6.Cg- Gt(ROSA)26Sortm14(CAGtdTomato)Hze/J Mouse LepRiDastro/GFAP+ Kim et al., 2014 B6.129P2-Leprtm1Rck/J;B6.Cg- Tg(GFAPcre/ERT2)505Fmv/J Mouse HIF-1aiDastro/GFAP+ This paper B6.129-Hif1atm3Rsjo/J;B6.Cg- Tg(GFAPcre/ERT2)505Fmv/J Mouse HIF-1aiDastro/GLAST+ This paper B6.129-Hif1atm3Rsjo/J;Slc1a3tm1(cre/ ERT2)Mgoe Mouse HIF1/ODD-Luciferase Jackson Laboratory FVB.129S6-Gt(ROSA)26Sortm2(HIF1A/luc) Keal/Js Mouse hGFAP.CreERT2 Dr. Vaccarino; Yale University; USA B6.Cg-Tg(GFAPcre/ERT2)505Fmv/J Mouse GLAST.CreERT2 Prof. Goetz; LMU/HMGU Munich; GER Slc1a3tm1(cre/ERT2)Mgoe Mouse ROSA26 mT/mG Jackson Laboratory B6.129(Cg)-Gt(ROSA)26Sortm4(ACTB- tdTomato,-EGFP)Luo/J Rat Wistar Kyoto Envigo WKY/NHsd Rat SHR (spontaneous hypertensive rat) Envigo SHR/NHsd Oligonucleotides VEGF shRNA targeting sequence 50 -TGA AGA TGT ACT CTA TCT CGT-30 Vector Biolabs Nt 1218-1238 of NM_009505 Hairpin loop sequence 50 -GTT TTG GCC ACT GAC TGAC-30 Vector Biolabs N/A VEGF164 overexpressing sequence Vector Biolabs RefSeq#:BC061468 LepRa forward primer 5 0 -GAA GTC TCT CAT GAC CAC TAC AGA TGA-30 Sigma-Aldrich N/A LepRa reverse primer 5 0 - TTG TTT CCC TCC ATC AAA ATG TAA -30 Sigma-Aldrich N/A (Continued on next page) e2 Cell Metabolism 33, 1155–1170.e1–e10, June 1, 2021 .. REAGENT or RESOURCE SOURCE IDENTIFIER LepRb forward primer 5 0 - GCA TGC AGA ATC AGT GAT ATT TGG-30 Sigma-Aldrich N/A LepRb reverse primer 5 0 - CAA GCT GTA TCG ACA CTG ATT TC TTC -30 Sigma-Aldrich N/A Software and algorithms FIJI NIH https://imagej.net/Fiji/Downloads FlowJo BD N/A GraphPad Prism 6.0 h GraphPad Software N/A IMARIS x64 9.1.2 Oxford instruments N/A MacLab Chart Pro 7.0 GE Healthcare N/A Other Bovine serum albumin Sigma Aldrich A8806 Gelatin from porcine skin (SUMI) VWR International SAFSG1890 Glo Lysis Buffer Promega E2661 Halt protease and phosphatase inhibitors (100x) Thermo Fisher Scientific, Waltham, USA 78446 Radioimmunoprecipitation assay (RIPA) buffer Sigma-Aldrich R0278-500ML AAV.Gfa2.iCre Vector Biolabs Cat. No.: VB1172 AAV.Gfa2.VEGFGFP Vector Biolabs Cat. No.: AAV-275992 AAV.Gfa2.EGFP Vector Biolabs Cat. No.: VB1180 Lectin-FITC conjugate Thermo Fisher L4895 WGA-Alexa Fluor 647 Thermo Fisher W32466 NeuroTrace 435/455 Thermo Fisher N21479 NeuroTrace 500/525 Thermo Fisher N21480 Albumin FITC Sigma Aldrich A9771 Dextran-FITC (40 kDa) Sigma Aldrich Cat# 53379

Article Title: Regional differences in progenitor metabolism shape brain growth during development.
Article Snippet: Article Regional differences in progenitor metabolism shape brain growth during development

Gene Expression:

Luciferase:

Enzyme-linked Immunosorbent Assay:

Bicinchoninic Acid Protein Assay:

Polymerase Chain Reaction:

cDNA Synthesis:

shRNA:

Sequencing:

Countercurrent Chromatography:

Incubation:

Article Title: Nucleoli and the nucleoli-centromere association are dynamic during normal development and in cancer
Article Snippet: .. The cells were fixed with 4% (weight/volume) paraformaldehyde in PBS for 15 minutes, washed with PBS, solubilized in 0.5% (volume/ volume) Triton X-100 (Sigma) in PBS for 10 minutes, washed, incubated in human CREST antibody at a dilution of 1:10, mouse myosin heavy chain MF-20 antibody at 1:100, or rabbit POLRIE antibody at 1:100 (Proteintech cat no: 16145-1-AP) for 1 hour, washed, and finally incubated in anti-human, anti-mouse, and anti-rabbit Alexa-fluor secondary antibodies at 1:200 (Invitrogen) for 1 hour. .. The cells were then mounted using Vectashield antifade mounting media with DAPI (Vector Laboratories, Inc. H-1200).

Neutralization:

Article Title: IL-13 protects from C. difficile Colitis.
Article Snippet: .. For anti-144 IL-13 neutralization, mice were administered 150 μg of anti-IL-13 (eBio1316H cat# 16-145 7135-85) or IgG (eBRG1 cat# 16430185) from Thermofisher, via IP injection on days 0 146 and 2 post-infection. ..

Review