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gpl570  (Thermo Fisher)


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    Structured Review

    Thermo Fisher gpl570
    Information of the microarray datasets.
    Gpl570, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+genome+u133a+array+microarray+data/CYTOSINE+%243-D-ARABINOFURANOSIDE+HYDROCHLORIDE+CRYSTALLINE/pmc11183325-2-2-4
    Average 93 stars, based on 1 article reviews
    gpl570 - by Bioz Stars, 2026-09
    93/100 stars

    Images

    1) Product Images from "Identification of apoptosis-immune-related gene signature and construction of diagnostic model for sepsis based on single-cell sequencing and bulk transcriptome analysis"

    Article Title: Identification of apoptosis-immune-related gene signature and construction of diagnostic model for sepsis based on single-cell sequencing and bulk transcriptome analysis

    Journal: Frontiers in Genetics

    doi: 10.3389/fgene.2024.1389630

    Information of the microarray datasets.
    Figure Legend Snippet: Information of the microarray datasets.

    Techniques Used: Microarray, Control

    Related Articles

    other:

    Article Title: A six-gene support vector machine classifier contributes to the diagnosis of pediatric septic shock
    Article Snippet: GSE13904 , GPL570 (HG-U133_Plus_2) Affymetrix Human Genome U133 Plus 2.0 Array , 227 , 18 , 67.

    Article Title: Neuropeptide diuretic hormone 31 mediates memory and sleep via distinct neural pathways in Drosophila.
    Article Snippet: Memory formation and sleep regulation are critical for brain functions in animals from invertebrates to humans.. Neuropeptides play a pivotal role in regulating physiological behaviors, including memory formation and sleep.. However, the detailed mechanisms by which neuropeptides regulate these physiological behaviors remains unclear.

    Single Particle:

    Article Title: Structure of endogenous inactive GID E3 ubiquitin ligase complex
    Article Snippet: .. Property Value Source EM reconstruction method SINGLE PARTICLE Depositor Imposed symmetry POINT, C1 Depositor Number of particles used 22718 Depositor Resolution determination method FSC 0.143 CUT-OFF Depositor CTF correction method Not provided Microscope FEI TALOS ARCTICA Depositor Voltage (kV) 200 Depositor Electron dose (e−/Å 2 ) 67.2 Depositor Minimum defocus (nm) Not provided Maximum defocus (nm) Not provided Magni cation Not provided Image detector FEI FALCON III (4k x 4k) Depositor Maximum map value 0.189 Depositor Minimum map value -0.096 Depositor Average map value 0.001 Depositor Map value standard deviation 0.007 Depositor Recommended contour level 0.0351 Depositor Map size (Å) 373.984, 373.984, 373.984 wwPDB Map dimensions 232, 232, 232 wwPDB Map angles (◦) 90.0, 90.0, 90.0 wwPDB Pixel spacing (Å) 1.612, 1.612, 1.612 Depositor ..

    Article Title: Cryo-EM structure of SARS-CoV-2 prototype spike protein receptor-binding domain in complex with hippopotamus ACE2
    Article Snippet: .. Property Value Source EM reconstruction method SINGLE PARTICLE Depositor Imposed symmetry Not Provided Number of particles used 227185 Depositor Resolution determination method FSC 0.143 CUT-OFF Depositor CTF correction method Not provided Microscope FEI TITAN KRIOS Depositor Voltage (kV) 300 Depositor Electron dose (e−/Å2) 60.0 Depositor Minimum defocus (nm) 1.0 Depositor Maximum defocus (nm) 2.0 Depositor Magnification Not provided Image detector GATAN K3 BIOQUANTUM (6k x 4k) Depositor Maximum map value 3.919 Depositor Minimum map value -2.171 Depositor Average map value 0.001 Depositor Map value standard deviation 0.043 Depositor Recommended contour level 0.35 Depositor Map size (Å) 380.80002, 380.80002, 380.80002 wwPDB Map dimensions 448, 448, 448 wwPDB Map angles (◦) 90.0, 90.0, 90.0 wwPDB Pixel spacing (Å) 0.85, 0.85, 0.85 Depositor ..

    Microscopy:

    Article Title: Structure of endogenous inactive GID E3 ubiquitin ligase complex
    Article Snippet: .. Property Value Source EM reconstruction method SINGLE PARTICLE Depositor Imposed symmetry POINT, C1 Depositor Number of particles used 22718 Depositor Resolution determination method FSC 0.143 CUT-OFF Depositor CTF correction method Not provided Microscope FEI TALOS ARCTICA Depositor Voltage (kV) 200 Depositor Electron dose (e−/Å 2 ) 67.2 Depositor Minimum defocus (nm) Not provided Maximum defocus (nm) Not provided Magni cation Not provided Image detector FEI FALCON III (4k x 4k) Depositor Maximum map value 0.189 Depositor Minimum map value -0.096 Depositor Average map value 0.001 Depositor Map value standard deviation 0.007 Depositor Recommended contour level 0.0351 Depositor Map size (Å) 373.984, 373.984, 373.984 wwPDB Map dimensions 232, 232, 232 wwPDB Map angles (◦) 90.0, 90.0, 90.0 wwPDB Pixel spacing (Å) 1.612, 1.612, 1.612 Depositor ..

    Article Title: Cryo-EM structure of SARS-CoV-2 prototype spike protein receptor-binding domain in complex with hippopotamus ACE2
    Article Snippet: .. Property Value Source EM reconstruction method SINGLE PARTICLE Depositor Imposed symmetry Not Provided Number of particles used 227185 Depositor Resolution determination method FSC 0.143 CUT-OFF Depositor CTF correction method Not provided Microscope FEI TITAN KRIOS Depositor Voltage (kV) 300 Depositor Electron dose (e−/Å2) 60.0 Depositor Minimum defocus (nm) 1.0 Depositor Maximum defocus (nm) 2.0 Depositor Magnification Not provided Image detector GATAN K3 BIOQUANTUM (6k x 4k) Depositor Maximum map value 3.919 Depositor Minimum map value -2.171 Depositor Average map value 0.001 Depositor Map value standard deviation 0.043 Depositor Recommended contour level 0.35 Depositor Map size (Å) 380.80002, 380.80002, 380.80002 wwPDB Map dimensions 448, 448, 448 wwPDB Map angles (◦) 90.0, 90.0, 90.0 wwPDB Pixel spacing (Å) 0.85, 0.85, 0.85 Depositor ..

    Standard Deviation:

    Article Title: Structure of endogenous inactive GID E3 ubiquitin ligase complex
    Article Snippet: .. Property Value Source EM reconstruction method SINGLE PARTICLE Depositor Imposed symmetry POINT, C1 Depositor Number of particles used 22718 Depositor Resolution determination method FSC 0.143 CUT-OFF Depositor CTF correction method Not provided Microscope FEI TALOS ARCTICA Depositor Voltage (kV) 200 Depositor Electron dose (e−/Å 2 ) 67.2 Depositor Minimum defocus (nm) Not provided Maximum defocus (nm) Not provided Magni cation Not provided Image detector FEI FALCON III (4k x 4k) Depositor Maximum map value 0.189 Depositor Minimum map value -0.096 Depositor Average map value 0.001 Depositor Map value standard deviation 0.007 Depositor Recommended contour level 0.0351 Depositor Map size (Å) 373.984, 373.984, 373.984 wwPDB Map dimensions 232, 232, 232 wwPDB Map angles (◦) 90.0, 90.0, 90.0 wwPDB Pixel spacing (Å) 1.612, 1.612, 1.612 Depositor ..

    Article Title: Cryo-EM structure of SARS-CoV-2 prototype spike protein receptor-binding domain in complex with hippopotamus ACE2
    Article Snippet: .. Property Value Source EM reconstruction method SINGLE PARTICLE Depositor Imposed symmetry Not Provided Number of particles used 227185 Depositor Resolution determination method FSC 0.143 CUT-OFF Depositor CTF correction method Not provided Microscope FEI TITAN KRIOS Depositor Voltage (kV) 300 Depositor Electron dose (e−/Å2) 60.0 Depositor Minimum defocus (nm) 1.0 Depositor Maximum defocus (nm) 2.0 Depositor Magnification Not provided Image detector GATAN K3 BIOQUANTUM (6k x 4k) Depositor Maximum map value 3.919 Depositor Minimum map value -2.171 Depositor Average map value 0.001 Depositor Map value standard deviation 0.043 Depositor Recommended contour level 0.35 Depositor Map size (Å) 380.80002, 380.80002, 380.80002 wwPDB Map dimensions 448, 448, 448 wwPDB Map angles (◦) 90.0, 90.0, 90.0 wwPDB Pixel spacing (Å) 0.85, 0.85, 0.85 Depositor ..

    Mutagenesis:

    Article Title: β-Catenin Causes Renal Dysplasia via Upregulation of Tgfβ2 and Dkk1
    Article Snippet: The reaction was visualized using AP-conju- gated secondary antibody (Cedarlane) and AP substrate to develop color reaction (Promega). .. WT and -catGOF-UB mutant whole kidneys were homogenized in T-PER tissue protein extraction reagent (Thermo Scientific) supplemented with Protease 728 Journal of the American Society of Nephrology J Am Soc Nephrol 22: 718–731, 2011 inhibitor cocktail (Thermo Scientific). .. For immunoblotting, primary anatibodies were directed against -catenin (Upstate; 1:1000) and GAPDH (Abcam; 1:1000).

    Protein Extraction:

    Article Title: β-Catenin Causes Renal Dysplasia via Upregulation of Tgfβ2 and Dkk1
    Article Snippet: The reaction was visualized using AP-conju- gated secondary antibody (Cedarlane) and AP substrate to develop color reaction (Promega). .. WT and -catGOF-UB mutant whole kidneys were homogenized in T-PER tissue protein extraction reagent (Thermo Scientific) supplemented with Protease 728 Journal of the American Society of Nephrology J Am Soc Nephrol 22: 718–731, 2011 inhibitor cocktail (Thermo Scientific). .. For immunoblotting, primary anatibodies were directed against -catenin (Upstate; 1:1000) and GAPDH (Abcam; 1:1000).

    Enzyme-linked Immunosorbent Assay:

    Article Title: Regulation of Foxo1 expression is critical for central B cell tolerance and allelic exclusion.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-B220 BioLegend Clone# RA3-6B2; RRID:AB_11203907 Anti-IgD BD Biosciences Clone# 11-26c-2a; RRID:AB_2738723 Anti-IgM eBioscience Clone# eB121-15F9; RRID:AB_10671539 Anti-IgM, polyclonal (for ELISA) SouthernBiotech Cat# 1021-01; RRID:AB_2687524 Anti-IgG1 (for ELISA) BioLegend or BD Biosciences Clone# A85-3; RRID:AB_394863 Anti-IgG2a (for ELISA) BioLegend or BD Biosciences Clone# RMG2a-62; RRID:AB_2563481 Anti-IgG2b (for ELISA) BioLegend or BD Biosciences Clone# RMG2b-1; RRID:AB_315065 Anti-IgG3 (for ELISA) BioLegend or BD Biosciences Clone# R2-38; RRID:AB_394841 Anti-CD43 BD Biosciences Clone# 1G10; RRID:AB_394748 Anti-CD21 BioLegend Clone# 7E9; RRID:AB_1953277 Anti-CD23 BD Biosciences Clone# B3B4; RRID:AB_2738526 Anti-CD24 BioLegend Clone# M1/69; RRID:AB_1595596 Anti-CD19 BD Biosciences Clone# 1D3; RRID:AB_2737915 Anti-Iglambda BioLegend Clone# RML-42; RRID:AB_961363 Anti-Iglambda SouthernBiotech Cat# 1060-02; RRID:AB_2794390 Anti-Igkappa BD Biosciences Clone# 187.1; RRID:AB_1727535 Anti-IL-7R BioLegend Clone# A7R34; RRID:AB_1937252 Anti-BAFFR R&D Systems Clone# BAF1357; RRID:AB_2271820 Anti-CXCR4 BioLegend Clone# L276F12; RRID:AB_2562785 Anti-CD93 eBioscience Clone# AA4.1; RID:AB_469466 Anti-CD1d BioLegend Clone# 1B1; RRID:AB_10683167 Anti-pH2AX BioLegend Clone# 2F3; RRID:AB_528919 Anti-pAKT (T308) Cell Signaling Technology Clone# C31E5E; RRID:AB_2255933 Anti-Foxo1 Cell Signaling Technology Clone# C29H4; RRID:AB_2106495 Anti-tri-methyl-histone H3 Cell Signaling Technology Clone# C36B11; RRID:AB_2799182 Anti-rabbit IgG-FITC SouthernBiotech Cat# 4050-02; RRID:AB_2795952 Anti-rabbit IgG-DyLight 649 BioLegend Cat# 406406; RRID:AB_1575135 Anti-3-83Ig(H + L) Produced in-house, University of Colorado Anschutz Medical Campus Clone# 54.1 Anti-B220 Microbeads Miltenyi Biotec Cat# 130-049-501 Anti-FITC Microbeads Miltenyi Biotec Cat# 130-058-701 Chemicals, peptides, and recombinant proteins Phosphate substrate Millipore Sigma #P5744 Streptavidin-AP SouthernBiotech #7105-05 Streptavidin-APC-Cy7 BD Biosciences #554063 BSA Fisher Scientific #BP1600-100 Critical commercial assays Zombie UV Fixable Viability Kit BioLegend #423108 eBioscienceTM Foxp3/Transcription Factor Staining Buffer Set eBioscience #00-5523-00 BioLegend Fixation Buffer BioLegend #420801 APC BrdU Flow Kit BD Pharmingen #552598 RNeasy PlusMini Kit Qiagen #74134 SuperScript IV VILO Master Mix Thermo Fisher Scientific #11756050 (Continued on next page) 16 Cell Reports 43, 114283, June 25, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Taqman Gene Expression Assay, Rag1, Mm01270936_m1 Thermo Fisher Scientific #4448892 Taqman Gene Expression Assay, Rag2, Mm00501300_m1 Thermo Fisher Scientific #4448892 Taqman Gene Expression Assay, Foxo1, Mm00490671_m1 Thermo Fisher Scientific #4331182 Taqman Gene Expression Assay, Cxcr4, Mm01996749_s1 Thermo Fisher Scientific #4453320 Taqman Gene Expression Assay, Cd79b, Mm00434143_m1 Thermo Fisher Scientific #4331182 Taqman Universal PCR Master Mix Applied Biosystems #4364338 Experimental models: Organisms/strains 3-83Igi,H-2d mice Braun et al74 Jackson Laboratories #011074 3-83Igi,H-2b mice Halverson et al26 N/A 3-83Igi,Rosa26-P110*,mb1-Cre mice Greaves et al31 N/A Rosa26-P110* mice Srinivasan et al55 Jackson Laboratories #012343 Mb1-Cre mice Hobeika et al75 Jackson Laboratories #029412 3-83Igi,Foxo1fl/fl,mb1-Cre mice This paper N/A Foxo1fl/fl mice Stephen Hedrick (UCSD) N/A 3-83Igi,Rosa26-Foxo1-AAA,mb1-Cre mice This paper N/A Foxo1-AAA mice Stephen Hedrick (UCSD) N/A 3-83Igi,Rosa26-P110*/Foxo1-AAA,mb1-Cre mice This paper N/A Software and algorithms Flowjo v10.9 BD https://www.flowjo.com/ GraphPad Prism 10 GraphPad https://www.graphpad.com/ QuantStudio 7 Flex Real-Time PCR System software Thermo Fisher Scientific https://www.thermofisher.com/ Other Fetal Bovine Serum Gemini #100106, Lot#050A07H00K

    Produced:

    Article Title: Regulation of Foxo1 expression is critical for central B cell tolerance and allelic exclusion.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-B220 BioLegend Clone# RA3-6B2; RRID:AB_11203907 Anti-IgD BD Biosciences Clone# 11-26c-2a; RRID:AB_2738723 Anti-IgM eBioscience Clone# eB121-15F9; RRID:AB_10671539 Anti-IgM, polyclonal (for ELISA) SouthernBiotech Cat# 1021-01; RRID:AB_2687524 Anti-IgG1 (for ELISA) BioLegend or BD Biosciences Clone# A85-3; RRID:AB_394863 Anti-IgG2a (for ELISA) BioLegend or BD Biosciences Clone# RMG2a-62; RRID:AB_2563481 Anti-IgG2b (for ELISA) BioLegend or BD Biosciences Clone# RMG2b-1; RRID:AB_315065 Anti-IgG3 (for ELISA) BioLegend or BD Biosciences Clone# R2-38; RRID:AB_394841 Anti-CD43 BD Biosciences Clone# 1G10; RRID:AB_394748 Anti-CD21 BioLegend Clone# 7E9; RRID:AB_1953277 Anti-CD23 BD Biosciences Clone# B3B4; RRID:AB_2738526 Anti-CD24 BioLegend Clone# M1/69; RRID:AB_1595596 Anti-CD19 BD Biosciences Clone# 1D3; RRID:AB_2737915 Anti-Iglambda BioLegend Clone# RML-42; RRID:AB_961363 Anti-Iglambda SouthernBiotech Cat# 1060-02; RRID:AB_2794390 Anti-Igkappa BD Biosciences Clone# 187.1; RRID:AB_1727535 Anti-IL-7R BioLegend Clone# A7R34; RRID:AB_1937252 Anti-BAFFR R&D Systems Clone# BAF1357; RRID:AB_2271820 Anti-CXCR4 BioLegend Clone# L276F12; RRID:AB_2562785 Anti-CD93 eBioscience Clone# AA4.1; RID:AB_469466 Anti-CD1d BioLegend Clone# 1B1; RRID:AB_10683167 Anti-pH2AX BioLegend Clone# 2F3; RRID:AB_528919 Anti-pAKT (T308) Cell Signaling Technology Clone# C31E5E; RRID:AB_2255933 Anti-Foxo1 Cell Signaling Technology Clone# C29H4; RRID:AB_2106495 Anti-tri-methyl-histone H3 Cell Signaling Technology Clone# C36B11; RRID:AB_2799182 Anti-rabbit IgG-FITC SouthernBiotech Cat# 4050-02; RRID:AB_2795952 Anti-rabbit IgG-DyLight 649 BioLegend Cat# 406406; RRID:AB_1575135 Anti-3-83Ig(H + L) Produced in-house, University of Colorado Anschutz Medical Campus Clone# 54.1 Anti-B220 Microbeads Miltenyi Biotec Cat# 130-049-501 Anti-FITC Microbeads Miltenyi Biotec Cat# 130-058-701 Chemicals, peptides, and recombinant proteins Phosphate substrate Millipore Sigma #P5744 Streptavidin-AP SouthernBiotech #7105-05 Streptavidin-APC-Cy7 BD Biosciences #554063 BSA Fisher Scientific #BP1600-100 Critical commercial assays Zombie UV Fixable Viability Kit BioLegend #423108 eBioscienceTM Foxp3/Transcription Factor Staining Buffer Set eBioscience #00-5523-00 BioLegend Fixation Buffer BioLegend #420801 APC BrdU Flow Kit BD Pharmingen #552598 RNeasy PlusMini Kit Qiagen #74134 SuperScript IV VILO Master Mix Thermo Fisher Scientific #11756050 (Continued on next page) 16 Cell Reports 43, 114283, June 25, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Taqman Gene Expression Assay, Rag1, Mm01270936_m1 Thermo Fisher Scientific #4448892 Taqman Gene Expression Assay, Rag2, Mm00501300_m1 Thermo Fisher Scientific #4448892 Taqman Gene Expression Assay, Foxo1, Mm00490671_m1 Thermo Fisher Scientific #4331182 Taqman Gene Expression Assay, Cxcr4, Mm01996749_s1 Thermo Fisher Scientific #4453320 Taqman Gene Expression Assay, Cd79b, Mm00434143_m1 Thermo Fisher Scientific #4331182 Taqman Universal PCR Master Mix Applied Biosystems #4364338 Experimental models: Organisms/strains 3-83Igi,H-2d mice Braun et al74 Jackson Laboratories #011074 3-83Igi,H-2b mice Halverson et al26 N/A 3-83Igi,Rosa26-P110*,mb1-Cre mice Greaves et al31 N/A Rosa26-P110* mice Srinivasan et al55 Jackson Laboratories #012343 Mb1-Cre mice Hobeika et al75 Jackson Laboratories #029412 3-83Igi,Foxo1fl/fl,mb1-Cre mice This paper N/A Foxo1fl/fl mice Stephen Hedrick (UCSD) N/A 3-83Igi,Rosa26-Foxo1-AAA,mb1-Cre mice This paper N/A Foxo1-AAA mice Stephen Hedrick (UCSD) N/A 3-83Igi,Rosa26-P110*/Foxo1-AAA,mb1-Cre mice This paper N/A Software and algorithms Flowjo v10.9 BD https://www.flowjo.com/ GraphPad Prism 10 GraphPad https://www.graphpad.com/ QuantStudio 7 Flex Real-Time PCR System software Thermo Fisher Scientific https://www.thermofisher.com/ Other Fetal Bovine Serum Gemini #100106, Lot#050A07H00K

    Recombinant:

    Article Title: Regulation of Foxo1 expression is critical for central B cell tolerance and allelic exclusion.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-B220 BioLegend Clone# RA3-6B2; RRID:AB_11203907 Anti-IgD BD Biosciences Clone# 11-26c-2a; RRID:AB_2738723 Anti-IgM eBioscience Clone# eB121-15F9; RRID:AB_10671539 Anti-IgM, polyclonal (for ELISA) SouthernBiotech Cat# 1021-01; RRID:AB_2687524 Anti-IgG1 (for ELISA) BioLegend or BD Biosciences Clone# A85-3; RRID:AB_394863 Anti-IgG2a (for ELISA) BioLegend or BD Biosciences Clone# RMG2a-62; RRID:AB_2563481 Anti-IgG2b (for ELISA) BioLegend or BD Biosciences Clone# RMG2b-1; RRID:AB_315065 Anti-IgG3 (for ELISA) BioLegend or BD Biosciences Clone# R2-38; RRID:AB_394841 Anti-CD43 BD Biosciences Clone# 1G10; RRID:AB_394748 Anti-CD21 BioLegend Clone# 7E9; RRID:AB_1953277 Anti-CD23 BD Biosciences Clone# B3B4; RRID:AB_2738526 Anti-CD24 BioLegend Clone# M1/69; RRID:AB_1595596 Anti-CD19 BD Biosciences Clone# 1D3; RRID:AB_2737915 Anti-Iglambda BioLegend Clone# RML-42; RRID:AB_961363 Anti-Iglambda SouthernBiotech Cat# 1060-02; RRID:AB_2794390 Anti-Igkappa BD Biosciences Clone# 187.1; RRID:AB_1727535 Anti-IL-7R BioLegend Clone# A7R34; RRID:AB_1937252 Anti-BAFFR R&D Systems Clone# BAF1357; RRID:AB_2271820 Anti-CXCR4 BioLegend Clone# L276F12; RRID:AB_2562785 Anti-CD93 eBioscience Clone# AA4.1; RID:AB_469466 Anti-CD1d BioLegend Clone# 1B1; RRID:AB_10683167 Anti-pH2AX BioLegend Clone# 2F3; RRID:AB_528919 Anti-pAKT (T308) Cell Signaling Technology Clone# C31E5E; RRID:AB_2255933 Anti-Foxo1 Cell Signaling Technology Clone# C29H4; RRID:AB_2106495 Anti-tri-methyl-histone H3 Cell Signaling Technology Clone# C36B11; RRID:AB_2799182 Anti-rabbit IgG-FITC SouthernBiotech Cat# 4050-02; RRID:AB_2795952 Anti-rabbit IgG-DyLight 649 BioLegend Cat# 406406; RRID:AB_1575135 Anti-3-83Ig(H + L) Produced in-house, University of Colorado Anschutz Medical Campus Clone# 54.1 Anti-B220 Microbeads Miltenyi Biotec Cat# 130-049-501 Anti-FITC Microbeads Miltenyi Biotec Cat# 130-058-701 Chemicals, peptides, and recombinant proteins Phosphate substrate Millipore Sigma #P5744 Streptavidin-AP SouthernBiotech #7105-05 Streptavidin-APC-Cy7 BD Biosciences #554063 BSA Fisher Scientific #BP1600-100 Critical commercial assays Zombie UV Fixable Viability Kit BioLegend #423108 eBioscienceTM Foxp3/Transcription Factor Staining Buffer Set eBioscience #00-5523-00 BioLegend Fixation Buffer BioLegend #420801 APC BrdU Flow Kit BD Pharmingen #552598 RNeasy PlusMini Kit Qiagen #74134 SuperScript IV VILO Master Mix Thermo Fisher Scientific #11756050 (Continued on next page) 16 Cell Reports 43, 114283, June 25, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Taqman Gene Expression Assay, Rag1, Mm01270936_m1 Thermo Fisher Scientific #4448892 Taqman Gene Expression Assay, Rag2, Mm00501300_m1 Thermo Fisher Scientific #4448892 Taqman Gene Expression Assay, Foxo1, Mm00490671_m1 Thermo Fisher Scientific #4331182 Taqman Gene Expression Assay, Cxcr4, Mm01996749_s1 Thermo Fisher Scientific #4453320 Taqman Gene Expression Assay, Cd79b, Mm00434143_m1 Thermo Fisher Scientific #4331182 Taqman Universal PCR Master Mix Applied Biosystems #4364338 Experimental models: Organisms/strains 3-83Igi,H-2d mice Braun et al74 Jackson Laboratories #011074 3-83Igi,H-2b mice Halverson et al26 N/A 3-83Igi,Rosa26-P110*,mb1-Cre mice Greaves et al31 N/A Rosa26-P110* mice Srinivasan et al55 Jackson Laboratories #012343 Mb1-Cre mice Hobeika et al75 Jackson Laboratories #029412 3-83Igi,Foxo1fl/fl,mb1-Cre mice This paper N/A Foxo1fl/fl mice Stephen Hedrick (UCSD) N/A 3-83Igi,Rosa26-Foxo1-AAA,mb1-Cre mice This paper N/A Foxo1-AAA mice Stephen Hedrick (UCSD) N/A 3-83Igi,Rosa26-P110*/Foxo1-AAA,mb1-Cre mice This paper N/A Software and algorithms Flowjo v10.9 BD https://www.flowjo.com/ GraphPad Prism 10 GraphPad https://www.graphpad.com/ QuantStudio 7 Flex Real-Time PCR System software Thermo Fisher Scientific https://www.thermofisher.com/ Other Fetal Bovine Serum Gemini #100106, Lot#050A07H00K

    Staining:

    Article Title: Regulation of Foxo1 expression is critical for central B cell tolerance and allelic exclusion.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-B220 BioLegend Clone# RA3-6B2; RRID:AB_11203907 Anti-IgD BD Biosciences Clone# 11-26c-2a; RRID:AB_2738723 Anti-IgM eBioscience Clone# eB121-15F9; RRID:AB_10671539 Anti-IgM, polyclonal (for ELISA) SouthernBiotech Cat# 1021-01; RRID:AB_2687524 Anti-IgG1 (for ELISA) BioLegend or BD Biosciences Clone# A85-3; RRID:AB_394863 Anti-IgG2a (for ELISA) BioLegend or BD Biosciences Clone# RMG2a-62; RRID:AB_2563481 Anti-IgG2b (for ELISA) BioLegend or BD Biosciences Clone# RMG2b-1; RRID:AB_315065 Anti-IgG3 (for ELISA) BioLegend or BD Biosciences Clone# R2-38; RRID:AB_394841 Anti-CD43 BD Biosciences Clone# 1G10; RRID:AB_394748 Anti-CD21 BioLegend Clone# 7E9; RRID:AB_1953277 Anti-CD23 BD Biosciences Clone# B3B4; RRID:AB_2738526 Anti-CD24 BioLegend Clone# M1/69; RRID:AB_1595596 Anti-CD19 BD Biosciences Clone# 1D3; RRID:AB_2737915 Anti-Iglambda BioLegend Clone# RML-42; RRID:AB_961363 Anti-Iglambda SouthernBiotech Cat# 1060-02; RRID:AB_2794390 Anti-Igkappa BD Biosciences Clone# 187.1; RRID:AB_1727535 Anti-IL-7R BioLegend Clone# A7R34; RRID:AB_1937252 Anti-BAFFR R&D Systems Clone# BAF1357; RRID:AB_2271820 Anti-CXCR4 BioLegend Clone# L276F12; RRID:AB_2562785 Anti-CD93 eBioscience Clone# AA4.1; RID:AB_469466 Anti-CD1d BioLegend Clone# 1B1; RRID:AB_10683167 Anti-pH2AX BioLegend Clone# 2F3; RRID:AB_528919 Anti-pAKT (T308) Cell Signaling Technology Clone# C31E5E; RRID:AB_2255933 Anti-Foxo1 Cell Signaling Technology Clone# C29H4; RRID:AB_2106495 Anti-tri-methyl-histone H3 Cell Signaling Technology Clone# C36B11; RRID:AB_2799182 Anti-rabbit IgG-FITC SouthernBiotech Cat# 4050-02; RRID:AB_2795952 Anti-rabbit IgG-DyLight 649 BioLegend Cat# 406406; RRID:AB_1575135 Anti-3-83Ig(H + L) Produced in-house, University of Colorado Anschutz Medical Campus Clone# 54.1 Anti-B220 Microbeads Miltenyi Biotec Cat# 130-049-501 Anti-FITC Microbeads Miltenyi Biotec Cat# 130-058-701 Chemicals, peptides, and recombinant proteins Phosphate substrate Millipore Sigma #P5744 Streptavidin-AP SouthernBiotech #7105-05 Streptavidin-APC-Cy7 BD Biosciences #554063 BSA Fisher Scientific #BP1600-100 Critical commercial assays Zombie UV Fixable Viability Kit BioLegend #423108 eBioscienceTM Foxp3/Transcription Factor Staining Buffer Set eBioscience #00-5523-00 BioLegend Fixation Buffer BioLegend #420801 APC BrdU Flow Kit BD Pharmingen #552598 RNeasy PlusMini Kit Qiagen #74134 SuperScript IV VILO Master Mix Thermo Fisher Scientific #11756050 (Continued on next page) 16 Cell Reports 43, 114283, June 25, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Taqman Gene Expression Assay, Rag1, Mm01270936_m1 Thermo Fisher Scientific #4448892 Taqman Gene Expression Assay, Rag2, Mm00501300_m1 Thermo Fisher Scientific #4448892 Taqman Gene Expression Assay, Foxo1, Mm00490671_m1 Thermo Fisher Scientific #4331182 Taqman Gene Expression Assay, Cxcr4, Mm01996749_s1 Thermo Fisher Scientific #4453320 Taqman Gene Expression Assay, Cd79b, Mm00434143_m1 Thermo Fisher Scientific #4331182 Taqman Universal PCR Master Mix Applied Biosystems #4364338 Experimental models: Organisms/strains 3-83Igi,H-2d mice Braun et al74 Jackson Laboratories #011074 3-83Igi,H-2b mice Halverson et al26 N/A 3-83Igi,Rosa26-P110*,mb1-Cre mice Greaves et al31 N/A Rosa26-P110* mice Srinivasan et al55 Jackson Laboratories #012343 Mb1-Cre mice Hobeika et al75 Jackson Laboratories #029412 3-83Igi,Foxo1fl/fl,mb1-Cre mice This paper N/A Foxo1fl/fl mice Stephen Hedrick (UCSD) N/A 3-83Igi,Rosa26-Foxo1-AAA,mb1-Cre mice This paper N/A Foxo1-AAA mice Stephen Hedrick (UCSD) N/A 3-83Igi,Rosa26-P110*/Foxo1-AAA,mb1-Cre mice This paper N/A Software and algorithms Flowjo v10.9 BD https://www.flowjo.com/ GraphPad Prism 10 GraphPad https://www.graphpad.com/ QuantStudio 7 Flex Real-Time PCR System software Thermo Fisher Scientific https://www.thermofisher.com/ Other Fetal Bovine Serum Gemini #100106, Lot#050A07H00K



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    Summary of the eight datasets included in the study.

    Journal: EBioMedicine

    Article Title: Development and validation of an immune gene-set based Prognostic signature in ovarian cancer

    doi: 10.1016/j.ebiom.2018.12.054

    Figure Lengend Snippet: Summary of the eight datasets included in the study.

    Article Snippet: For TCGA cohort, due to the samples with available RNA-Sequencing gene expression quantification were relatively small ( n = 374), we collected Affymetrix Human Genome U133A Array microarray data instead ( n = 519).

    Techniques: Microarray