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circrna芯片 human circular rna array v2.0  (Arraystar inc)

 
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    Arraystar inc circrna芯片 human circular rna array v2.0
    Circrna芯片 Human Circular Rna Array V2.0, supplied by Arraystar inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+arraystar+circrna+array/circrna%E8%8A%AF%E7%89%87+human+circular+rna+array+v2+0/pmc06744033-63-0-7
    Average 90 stars, based on 1 article reviews
    circrna芯片 human circular rna array v2.0 - by Bioz Stars, 2026-09
    90/100 stars

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    Article Title: Luminal亚型乳腺癌细胞与正常乳腺细胞的circRNA表达谱差异分析
    Article Snippet: 根据Arraystar公司提供的circRNA芯片(Human circular RNA Array V2.0)进行样本标记和序列杂交:用RNase R(Epeentre Technologies, USA)酶解总RNA以除去线性RNA并富集环状RNA,利用随机引物法(Arraystar Super RNA标记试剂盒,Arraystar)扩增circRNA并转录成荧光标记的cRNA;通过RNeasy Mini Kit(Qiagen)对标记的cRNA进行纯化;利用NanoDrop ND-1000测量cRNA(pmol Cy3/μg cRNA)的浓度和比活性;将标记的cRNA与circRNA芯片杂交:在1 μg标记的cRNA中加入5 μL 10×封闭剂和1 μL 25×裂解缓冲液进行片段化,60 °C孵育30 min,加入25 μL 2×杂交缓冲液稀释标记物的cRNA;将50 μL杂交溶液滴加到circRNA表达微阵列载玻片上,在杂交炉中65 °C孵育17 h;最后用Agilent Scanner G2505C洗涤玻片,固定并扫描。



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    Image Search Results


    Circular RNAs are deregulated in human abdominal aortic aneurysm (A) Volcano plot depicting downregulated (51, blue) and upregulated (40, red). Circular RNAs (circRNAs) in human elective human abdominal aortic aneurysm (eAAA, n = 11) vs . control (CTRL, n = 6) aorta specimens, as resulted by array experiments. Log2 fold change and -log10 p value are plotted on the x and y axes, respectively. IDs of circRNAs meant for a first round of validation are highlighted. Statistics: unpaired t test; p values <0.05 are considered significant. (B) Pie chart illustrating the proportion of exonic (89.8%), intronic (5.7%), sense overlapping (3.4%), and antisense (1.1%) array-identified differentially expressed circRNAs. Absolute numbers are further indicated for each group. (C) Real-time quantitative PCR (qPCR) validation of hsa_circ_0005660 (c NFIX ), hsa_circ_0003641 (c ATM ), hsa_circ0042103 (c MYOCD ), hsa_circ003218 (c BMPR2 ), hsa_circ0004771 (c NRIP1 ), and hsa_circ0005615 (c NFATC3 ) differential expression in human eAAA (N = 8) and CTRL (N = 4) aortas. 2 –ddCT was calculated by normalizing on RPLPO . Data are represented as mean ± SEM. Statistics: unpaired t test; p values <0.05 are considered significant. NS, not significant; eAAA, elective AAA.

    Journal: Molecular Therapy. Nucleic Acids

    Article Title: The circular RNA Ataxia Telangiectasia Mutated regulates oxidative stress in smooth muscle cells in expanding abdominal aortic aneurysms

    doi: 10.1016/j.omtn.2023.08.017

    Figure Lengend Snippet: Circular RNAs are deregulated in human abdominal aortic aneurysm (A) Volcano plot depicting downregulated (51, blue) and upregulated (40, red). Circular RNAs (circRNAs) in human elective human abdominal aortic aneurysm (eAAA, n = 11) vs . control (CTRL, n = 6) aorta specimens, as resulted by array experiments. Log2 fold change and -log10 p value are plotted on the x and y axes, respectively. IDs of circRNAs meant for a first round of validation are highlighted. Statistics: unpaired t test; p values <0.05 are considered significant. (B) Pie chart illustrating the proportion of exonic (89.8%), intronic (5.7%), sense overlapping (3.4%), and antisense (1.1%) array-identified differentially expressed circRNAs. Absolute numbers are further indicated for each group. (C) Real-time quantitative PCR (qPCR) validation of hsa_circ_0005660 (c NFIX ), hsa_circ_0003641 (c ATM ), hsa_circ0042103 (c MYOCD ), hsa_circ003218 (c BMPR2 ), hsa_circ0004771 (c NRIP1 ), and hsa_circ0005615 (c NFATC3 ) differential expression in human eAAA (N = 8) and CTRL (N = 4) aortas. 2 –ddCT was calculated by normalizing on RPLPO . Data are represented as mean ± SEM. Statistics: unpaired t test; p values <0.05 are considered significant. NS, not significant; eAAA, elective AAA.

    Article Snippet: The resulting labeled cDNA was then purified and 1 μg was fragmented, heated, and subsequently hybridized with an 8 × 15k commercially available array chip displaying 13,617 human circRNAs (Arraystar, no. AS-S-CR-H-V2.0) for 17 h at 65°C in an Agilent Hybridization Oven.

    Techniques: Control, Biomarker Discovery, Real-time Polymerase Chain Reaction, Quantitative Proteomics