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circular rna expression profiling arraystar mouse circrna array v2  (Arraystar inc)

 
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    Arraystar inc circular rna expression profiling arraystar mouse circrna array v2
    a Scatter plots were used to evaluate the difference in the expression of circRNAs between Ang II and control groups. The values plotted on X and Y axes are the averaged normalized signal values of each group (log2 scaled). The circRNAs above the top green line and below the bottom green line indicate >1.5-fold change between the two groups. b Hierarchical clustering analysis showed the differentially expressed circRNAs over 2.0-fold change. Red color indicates high expression level, and blue color indicates low expression level. c Divergent and convergent primers were used to verify whether circNRG-1 was a <t>circRNA.</t> Convergent primers were used to detect NRG-1 mRNA. Divergent primers amplified circNRG-1 in cDNA but not gDNA. GAPDH served as linear control and size marker in base pairs. d Sanger sequencing confirmed head-to-tail junction of circNRG-1. e RNA fluorescence in situ hybridization for circNRG-1 was detected. Nuclei were stained with DAPI. Scale bars = 50 μm. f qRT-PCR detected circNRG-1 expression in MASMCs treated with Ang II (10 −7 M) for the different times. Data represent the means ± SEM of three independent experiments. * P < 0.05, *** P < 0.001 vs . Ang II for 0 h
    Circular Rna Expression Profiling Arraystar Mouse Circrna Array V2, supplied by Arraystar inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+arraystar+circrna+array/circrna+microarray+arraystar+human+circrna+array+v2/pmc06494886-56-8-13
    Average 90 stars, based on 1 article reviews
    circular rna expression profiling arraystar mouse circrna array v2 - by Bioz Stars, 2026-10
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    Images

    1) Product Images from "Angiotensin II inhibits apoptosis of mouse aortic smooth muscle cells through regulating the circNRG-1/miR-193b-5p/NRG-1 axis"

    Article Title: Angiotensin II inhibits apoptosis of mouse aortic smooth muscle cells through regulating the circNRG-1/miR-193b-5p/NRG-1 axis

    Journal: Cell Death & Disease

    doi: 10.1038/s41419-019-1590-5

    a Scatter plots were used to evaluate the difference in the expression of circRNAs between Ang II and control groups. The values plotted on X and Y axes are the averaged normalized signal values of each group (log2 scaled). The circRNAs above the top green line and below the bottom green line indicate >1.5-fold change between the two groups. b Hierarchical clustering analysis showed the differentially expressed circRNAs over 2.0-fold change. Red color indicates high expression level, and blue color indicates low expression level. c Divergent and convergent primers were used to verify whether circNRG-1 was a circRNA. Convergent primers were used to detect NRG-1 mRNA. Divergent primers amplified circNRG-1 in cDNA but not gDNA. GAPDH served as linear control and size marker in base pairs. d Sanger sequencing confirmed head-to-tail junction of circNRG-1. e RNA fluorescence in situ hybridization for circNRG-1 was detected. Nuclei were stained with DAPI. Scale bars = 50 μm. f qRT-PCR detected circNRG-1 expression in MASMCs treated with Ang II (10 −7 M) for the different times. Data represent the means ± SEM of three independent experiments. * P < 0.05, *** P < 0.001 vs . Ang II for 0 h
    Figure Legend Snippet: a Scatter plots were used to evaluate the difference in the expression of circRNAs between Ang II and control groups. The values plotted on X and Y axes are the averaged normalized signal values of each group (log2 scaled). The circRNAs above the top green line and below the bottom green line indicate >1.5-fold change between the two groups. b Hierarchical clustering analysis showed the differentially expressed circRNAs over 2.0-fold change. Red color indicates high expression level, and blue color indicates low expression level. c Divergent and convergent primers were used to verify whether circNRG-1 was a circRNA. Convergent primers were used to detect NRG-1 mRNA. Divergent primers amplified circNRG-1 in cDNA but not gDNA. GAPDH served as linear control and size marker in base pairs. d Sanger sequencing confirmed head-to-tail junction of circNRG-1. e RNA fluorescence in situ hybridization for circNRG-1 was detected. Nuclei were stained with DAPI. Scale bars = 50 μm. f qRT-PCR detected circNRG-1 expression in MASMCs treated with Ang II (10 −7 M) for the different times. Data represent the means ± SEM of three independent experiments. * P < 0.05, *** P < 0.001 vs . Ang II for 0 h

    Techniques Used: Expressing, Control, Amplification, Marker, Sequencing, Fluorescence, In Situ Hybridization, Staining, Quantitative RT-PCR

    The orange, purple and green nodes represent circRNA, miRNA and mRNA respectively. Markers highlighting staining showed circNRG-1-miR-193b-5p-NRG-1 interactions
    Figure Legend Snippet: The orange, purple and green nodes represent circRNA, miRNA and mRNA respectively. Markers highlighting staining showed circNRG-1-miR-193b-5p-NRG-1 interactions

    Techniques Used: Staining

    Related Articles

    Microarray:

    Article Title: CircMETTL3-156aa reshapes the glycolytic metabolism of macrophages to promote M1 polarization and induce cytokine storms in sHLH.
    Article Snippet: The sample preparation and microarray hybridization were performed based on Arraystar’s standard protocols. .. A circRNA microarray (Arraystar Human circRNAs chip, ArrayStar) containing more than 14000 probes specific for splice sites in human circRNAs was used in this study. ..

    Article Title: Screening and validating circular RNAs that estimate disease risk and treatment response of pediatric acute myeloid leukemia: a microarray-based analyses and RT-qPCR validation.
    Article Snippet: Purpose Circular RNA (circRNA) is a type of novel non-coding RNA with close involvement in the tumorigenesis and treatment response of leukemias.. This study aimed to screen and validate candidate circRNAs that estimate disease risk and response to induction therapy of pediatric acute myeloid leukemia (AML).. Methods Bone marrow samples were obtained from 4 complete response (CR) pediatric AML patients, 4 non-CR pediatric AML patients, and 4 controls to screen differentially expressed circRNAs (DECs) through microarray analyses.

    Article Title: Role of p53/circRNA0085439/Ku70 axis in DNA damage response in lung cells exposed to ZnO nanoparticles: Involvement of epigenetic regulation
    Article Snippet: .. To explore the alterations of circRNAs between p53 wild-type control group and p53-knockout group, circRNA microarray (Arraystar Human circRNAs chip, ArrayStar) containing more than 5000 probes specific for splice sites in human circRNAs was used in this study provided by OEbiotech Bio-Tech Inc (https:// www. oebio tech. com/). ..

    Article Title: Comprehensive analysis of circRNA-miRNA-mRNA in oral squamous cell carcinoma.
    Article Snippet: Objectives: The aim of the present study was to explore expression profiles of circular RNAs (circRNAs) and potential molecular mechanisms in oral squamous cell carcinoma (OSCC).. Design: In this study, high-throughput microarray was used to detect the expression profiles of circRNAs in OSCC.. Reverse transcription quantitative PCR (RT-qPCR) was used to quantify the expression of dysregulated circRNAs in microarray.

    Article Title: A Plasma Circular RNA Profile Differentiates Subjects with Alzheimer's Disease and Mild Cognitive Impairment from Healthy Controls.
    Article Snippet: .. Thus, 50 μL of sample mixture was dispensed into the gasket slide and assembled to the circRNA expression Arraystar Human circRNA Array V2 (8×15K, Arraystar Inc.) microarray slide. .. The slides were incubated for 17 h at 65 ◦C in an Agilent Hybridization Oven.

    Article Title: The circular RNA circTXNRD1 promoted ambient particulate matter-induced inflammation in human bronchial epithelial cells by regulating miR-892a/COX-2 axis.
    Article Snippet: .. Then, the expression level of circRNA in each sample was detected using a human circRNA microarray (Arraystar Human circRNA Array V2, 8 × 15 K, Arraystar; Kangchen Biotech, Shanghai, China), as the previous study reported (Su et al., 2016). .. The raw data was extracted using Agilent Feature Extraction software (version 11.0.1.1).

    Article Title: CircRNF220, not its linear cognate gene RNF220, regulates cell growth and is associated with relapse in pediatric acute myeloid leukemia
    Article Snippet: .. A circRNA microarray (Arraystar Human circRNAs chip, ArrayStar) containing more than 5000 probes specific for splice sites in human circRNAs was used in this study. .. After hybridization, 5 pediatric AML samples (pooled) and 5 healthy donor BM samples (pooled) were examined using the circRNA microarray provided by Kangcheng Bio-Tech Inc. R software was used to process the subsequent data after quantile normalization.

    other:

    Article Title: Identification and validation of disease severity-related circular RNA in acute pancreatitis.
    Article Snippet: To explore the role of circular RNA in acute pancreatitis, we employed circRNA microarray technology (Arraystar Human circRNA Array V2) to examine the circRNA expression profile in the blood of three acute pancreatitis patients and three healthy controls.

    Control:

    Article Title: Role of p53/circRNA0085439/Ku70 axis in DNA damage response in lung cells exposed to ZnO nanoparticles: Involvement of epigenetic regulation
    Article Snippet: .. To explore the alterations of circRNAs between p53 wild-type control group and p53-knockout group, circRNA microarray (Arraystar Human circRNAs chip, ArrayStar) containing more than 5000 probes specific for splice sites in human circRNAs was used in this study provided by OEbiotech Bio-Tech Inc (https:// www. oebio tech. com/). ..

    Expressing:

    Article Title: Comprehensive analysis of circRNA-miRNA-mRNA in oral squamous cell carcinoma.
    Article Snippet: Objectives: The aim of the present study was to explore expression profiles of circular RNAs (circRNAs) and potential molecular mechanisms in oral squamous cell carcinoma (OSCC).. Design: In this study, high-throughput microarray was used to detect the expression profiles of circRNAs in OSCC.. Reverse transcription quantitative PCR (RT-qPCR) was used to quantify the expression of dysregulated circRNAs in microarray.

    Article Title: A Plasma Circular RNA Profile Differentiates Subjects with Alzheimer's Disease and Mild Cognitive Impairment from Healthy Controls.
    Article Snippet: .. Thus, 50 μL of sample mixture was dispensed into the gasket slide and assembled to the circRNA expression Arraystar Human circRNA Array V2 (8×15K, Arraystar Inc.) microarray slide. .. The slides were incubated for 17 h at 65 ◦C in an Agilent Hybridization Oven.

    Article Title: The circular RNA circTXNRD1 promoted ambient particulate matter-induced inflammation in human bronchial epithelial cells by regulating miR-892a/COX-2 axis.
    Article Snippet: .. Then, the expression level of circRNA in each sample was detected using a human circRNA microarray (Arraystar Human circRNA Array V2, 8 × 15 K, Arraystar; Kangchen Biotech, Shanghai, China), as the previous study reported (Su et al., 2016). .. The raw data was extracted using Agilent Feature Extraction software (version 11.0.1.1).

    High Throughput Screening Assay:

    Article Title: Comprehensive analysis of circRNA-miRNA-mRNA in oral squamous cell carcinoma.
    Article Snippet: Objectives: The aim of the present study was to explore expression profiles of circular RNAs (circRNAs) and potential molecular mechanisms in oral squamous cell carcinoma (OSCC).. Design: In this study, high-throughput microarray was used to detect the expression profiles of circRNAs in OSCC.. Reverse transcription quantitative PCR (RT-qPCR) was used to quantify the expression of dysregulated circRNAs in microarray.



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    Image Search Results


    Circular RNAs are deregulated in human abdominal aortic aneurysm (A) Volcano plot depicting downregulated (51, blue) and upregulated (40, red). Circular RNAs (circRNAs) in human elective human abdominal aortic aneurysm (eAAA, n = 11) vs . control (CTRL, n = 6) aorta specimens, as resulted by array experiments. Log2 fold change and -log10 p value are plotted on the x and y axes, respectively. IDs of circRNAs meant for a first round of validation are highlighted. Statistics: unpaired t test; p values <0.05 are considered significant. (B) Pie chart illustrating the proportion of exonic (89.8%), intronic (5.7%), sense overlapping (3.4%), and antisense (1.1%) array-identified differentially expressed circRNAs. Absolute numbers are further indicated for each group. (C) Real-time quantitative PCR (qPCR) validation of hsa_circ_0005660 (c NFIX ), hsa_circ_0003641 (c ATM ), hsa_circ0042103 (c MYOCD ), hsa_circ003218 (c BMPR2 ), hsa_circ0004771 (c NRIP1 ), and hsa_circ0005615 (c NFATC3 ) differential expression in human eAAA (N = 8) and CTRL (N = 4) aortas. 2 –ddCT was calculated by normalizing on RPLPO . Data are represented as mean ± SEM. Statistics: unpaired t test; p values <0.05 are considered significant. NS, not significant; eAAA, elective AAA.

    Journal: Molecular Therapy. Nucleic Acids

    Article Title: The circular RNA Ataxia Telangiectasia Mutated regulates oxidative stress in smooth muscle cells in expanding abdominal aortic aneurysms

    doi: 10.1016/j.omtn.2023.08.017

    Figure Lengend Snippet: Circular RNAs are deregulated in human abdominal aortic aneurysm (A) Volcano plot depicting downregulated (51, blue) and upregulated (40, red). Circular RNAs (circRNAs) in human elective human abdominal aortic aneurysm (eAAA, n = 11) vs . control (CTRL, n = 6) aorta specimens, as resulted by array experiments. Log2 fold change and -log10 p value are plotted on the x and y axes, respectively. IDs of circRNAs meant for a first round of validation are highlighted. Statistics: unpaired t test; p values <0.05 are considered significant. (B) Pie chart illustrating the proportion of exonic (89.8%), intronic (5.7%), sense overlapping (3.4%), and antisense (1.1%) array-identified differentially expressed circRNAs. Absolute numbers are further indicated for each group. (C) Real-time quantitative PCR (qPCR) validation of hsa_circ_0005660 (c NFIX ), hsa_circ_0003641 (c ATM ), hsa_circ0042103 (c MYOCD ), hsa_circ003218 (c BMPR2 ), hsa_circ0004771 (c NRIP1 ), and hsa_circ0005615 (c NFATC3 ) differential expression in human eAAA (N = 8) and CTRL (N = 4) aortas. 2 –ddCT was calculated by normalizing on RPLPO . Data are represented as mean ± SEM. Statistics: unpaired t test; p values <0.05 are considered significant. NS, not significant; eAAA, elective AAA.

    Article Snippet: The resulting labeled cDNA was then purified and 1 μg was fragmented, heated, and subsequently hybridized with an 8 × 15k commercially available array chip displaying 13,617 human circRNAs (Arraystar, no. AS-S-CR-H-V2.0) for 17 h at 65°C in an Agilent Hybridization Oven.

    Techniques: Control, Biomarker Discovery, Real-time Polymerase Chain Reaction, Quantitative Proteomics