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programmable horizontal puller p-87  (Sutter Instrument Company)


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    Structured Review

    Sutter Instrument Company programmable horizontal puller p-87
    Programmable Horizontal Puller P 87, supplied by Sutter Instrument Company, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/horizontal+programmable+puller/micropipette+puller+p+97/pm36068614-361-13-17
    Average 90 stars, based on 1 article reviews
    programmable horizontal puller p-87 - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Microscopy:

    Article Title: Connectivity of a Conspicuous Layered Structure in the Dorsal Telencephalon of the Peacock Gudgeon, Tateurndina ocellicauda
    Article Snippet: The tissue was then dipped into ice‐cold Ringer's solution and washed in Ringer's solution twice for 10 min. A fluorescent stereomicroscope (Nikon SMZ 25, Minato City, Tokyo, Japan) was used to visualize the respective tracer application sites. .. Injections were performed by pulling glass microelectrodes with a micropipette puller (P‐87, Sutter Instruments, California, USA) using borosilicate glass with filament (GB150F‐8P, Science Products, Hofheim, Germany) and with their tips broken back under a microscope. .. These were filled with 10% neurobiotin (Vector Laboratories Inc., Burlingame, CA, USA) in 0.5 M potassium acetate containing 0.05% pyranine (Thermo Fisher, Kandel, Germany) to visualize the electrode tip (10 MΩ resistance).

    Article Title: Connectivity of a Conspicuous Layered Structure in the Dorsal Telencephalon of the Peacock Gudgeon, Tateurndina ocellicauda.
    Article Snippet: The tissue was then dipped into ice-cold Ringer’s solution and washed in Ringer’s solution twice for 10 min. A fluorescent stereomicroscope (Nikon SMZ 25, Minato City, Tokyo, Japan) was used to visualize the respective tracer application sites. .. Injections were performed by pulling glass microelectrodes with a micropipette puller (P-87, Sutter Instruments, California, USA) using borosilicate glass with filament (GB150F-8P, Science Products, Hofheim, Germany) andwith their tips broken back under a microscope. .. These were filled with 10% neurobiotin (Vector Laboratories Inc., Burlingame, CA, USA) in 0.5 M potassium acetate containing 0.05% pyranine (Thermo Fisher, Kandel, Germany) to visualize the electrode tip (10 MΩ resistance).

    Concentration Assay:

    Article Title: Anti-VEGF antibody constructs
    Article Snippet: .. Introduction of rAAV particles was performed through the following steps: A) preauricular incision to expose the cochlear bulla, B) glass micropipettes (cat #4878—WPI) pulled with a micropipette puller (cat #P87—Sutter instruments) to a final OD of about 10 μm were used to manually deliver (micropipettes held by a Nanoliter 2000 micromanipulator—WPI) compositions containing rAAV particles into the scala tympanic, which allows access to inner ear cells, C) 1 μL of a composition described herein at a suitable concentration in suitable buffer (described in Example 1, rAAVAnc80-ranibizumab-PC particles at approximately 1.4×1010 vg/per cochlea, or rAAVAnc80-bevacizumab-PC particles at approximately 1.2×1010 vg/per cochlea; each diluted in in 1×PBS with pluronic acid F68) was injected into each tested cochlea at a release rate of 0.3 μL/min (controlled by MICRO4 microinjection controller—WPI). ..

    Injection:

    Article Title: Anti-VEGF antibody constructs
    Article Snippet: .. Introduction of rAAV particles was performed through the following steps: A) preauricular incision to expose the cochlear bulla, B) glass micropipettes (cat #4878—WPI) pulled with a micropipette puller (cat #P87—Sutter instruments) to a final OD of about 10 μm were used to manually deliver (micropipettes held by a Nanoliter 2000 micromanipulator—WPI) compositions containing rAAV particles into the scala tympanic, which allows access to inner ear cells, C) 1 μL of a composition described herein at a suitable concentration in suitable buffer (described in Example 1, rAAVAnc80-ranibizumab-PC particles at approximately 1.4×1010 vg/per cochlea, or rAAVAnc80-bevacizumab-PC particles at approximately 1.2×1010 vg/per cochlea; each diluted in in 1×PBS with pluronic acid F68) was injected into each tested cochlea at a release rate of 0.3 μL/min (controlled by MICRO4 microinjection controller—WPI). ..

    Microinjection:

    Article Title: Anti-VEGF antibody constructs
    Article Snippet: .. Introduction of rAAV particles was performed through the following steps: A) preauricular incision to expose the cochlear bulla, B) glass micropipettes (cat #4878—WPI) pulled with a micropipette puller (cat #P87—Sutter instruments) to a final OD of about 10 μm were used to manually deliver (micropipettes held by a Nanoliter 2000 micromanipulator—WPI) compositions containing rAAV particles into the scala tympanic, which allows access to inner ear cells, C) 1 μL of a composition described herein at a suitable concentration in suitable buffer (described in Example 1, rAAVAnc80-ranibizumab-PC particles at approximately 1.4×1010 vg/per cochlea, or rAAVAnc80-bevacizumab-PC particles at approximately 1.2×1010 vg/per cochlea; each diluted in in 1×PBS with pluronic acid F68) was injected into each tested cochlea at a release rate of 0.3 μL/min (controlled by MICRO4 microinjection controller—WPI). ..

    other:

    Article Title: Responses of inferior colliculus neurons to notched noises in awake mice: putative neural correlates of auditory enhancement and Zwicker tone
    Article Snippet: Electrodes were fabricated with a P-2000 horizontal micropipette puller (Sutter Instrument) and positioned using a precision digital micromanipulator (MP-225, Sutter Instrument) under a surgical microscope (Leica MZ9.5).

    Article Title: Alcohol disrupts long-term potentiation at hippocampus-medium spiny neuron synapses in the medial shell of the nucleus accumbens
    Article Snippet: Patch pipettes (4-8MΩ) were made from borosilicate glass (World Precision Instruments) using a Sutter Instruments P-97 model puller.

    Article Title: Highly sensitive and rapid screening of radical electrochemical intermediates by chemical labeling untargeted mass spectrometry.
    Article Snippet: Capturing and analyzing low-abundance, short-lived, and unstable radical intermediates during electrochemical (EC) reactions is important for understanding EC reaction mechanisms.. Herein, we developed a chemical labeling strategy for rapid screening and highly sensitive analysis of EC radical intermediates by mass spectrometry (MS).. Taking 5,5-dimethyl-1-pyrroline N-oxide (DMPO) labeling reagent as an example, DMPO can react with radical intermediates to form stable labeling products with characteristic fragmentation daughter ions or neutral loss in tandem MS, so the labeled intermediates can be screened by precursor ions scan or neutral loss scan.



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