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innoscan 710 high resolution microarray scanner  (Innopsys Inc)

 
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    Structured Review

    Innopsys Inc innoscan 710 high resolution microarray scanner
    Innoscan 710 High Resolution Microarray Scanner, supplied by Innopsys Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/high+microarray+scanner/710al+innopsys+scanner/pmc13207355-405-10-15
    Average 86 stars, based on 1 article reviews
    innoscan 710 high resolution microarray scanner - by Bioz Stars, 2026-11
    86/100 stars

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    Related Articles

    Microarray:

    Article Title: Co-enrichment of proteins in extracellular vesicles.
    Article Snippet: The slides were incubated at room temperature on a shaker for 2 h. The detection antibodies were detected using either anti-species fluorescently-labelled secondary antibodies, fluorescently labelled streptavidin at a final concentration of 1 μg/mL diluted in 0.03% PBST with 3% BSA, or fluorescently labelled DNA oligos at a final concentration of 10 nM in PBS with 0.025% Tween- AR TI CL E IN P RE SS 20, 300 mM NaCl, 0.5 mg/mL sheared salmon sperm DNA (Thermo Fisher, USA) and 0.5% dextran sulfate (Sigma, USA).31 For intravesicular targets, an additional fixation (10 min with 4% paraformaldehyde), antigen retrieval (1 min with 90°C PBS), and quenching step (2 h with 0.03%PBST, 3% BSA and 0.3M glycine) were performed prior to detection antibody incubation.31 Finally, the slides were washed in a 0.03% PBST bath for 15 min (or 5 min for oligo- detected targets) on a shaker, dried using a centrifugal slide dryer before imaging. .. A microarray scanner (InnoScan 1100 AL, Innopsys, France) was used to visualize the microarray slides, where the gain and laser power were made consistent across the same experiment. .. ArrayPro 4.5 (Meyer Instruments, USA) was used to quantify the mean relative fluorescence unit (RFU) value for each microarray spots.

    Article Title: Conserved genetic and antigenic profiles of Pf12 and Pf41 in asymptomatic Plasmodium falciparum carriers in Tanzania.
    Article Snippet: .. Bound IgG antibodies were detected using Alexa Fluor 546-conjugated goat antihuman IgG (10 ng/μL; Invitrogen), and fluorescence signals were acquired using the InnoScan 300 microarray scanner (INNOPSYS, Carbonne, France). ..

    Incubation:

    Article Title: Astrocytes transform the transcriptomic and epigenetic landscape of developing cortical neurons to promote activity via intrinsic excitability control
    Article Snippet: .. 120 antibodies were profiled using standard procedures, 1 hour incubation with primary followed by 30 min incubation with Dylight-800 anti-species secondary antibodies, with arrays imaged using an Innopsys Innoscan 710 scanner. ..

    Article Title: A comprehensive pharmacological survey across heterogeneous patient-derived glioblastoma stem cell models
    Article Snippet: Clamps were removed and microarrays were washed three times for 5 min in PBS-T. Microarrays were then blocked for 10min with Super G Blocking Buffer at room temperature and again washed three times for 5 min in PBS-T. Three millilitres (3 mL) of IRDye 800CW Streptavidin (LI-COR Biosciences) was diluted 1:5000 in PBS-T supplemented with 5% BSA, 10% SuperG Blocking Buffer. .. Following blocking buffer (Superblock T20) for 10 min the detection of marker antibodies was performed in a two-step sequential assay (1) incubation of an array with primary analyte-specific antibody in blocking buffer for 60 min at room temperature and (2) removal of excess antibody by washing arrays with PBS-T, followed by a further incubation with blocking buffer and PBS-T washes, and incubation secondary antibody (Dylight-800-labeled anti-species antibodies diluted 1:2500 in Superblock T20) for 30 min. After further washing and slide drying, the arrays were imaged in the Innopsys Innoscan 710 scanner. .. Microarrays were dried then scanned at 795 nm on the InnoScan 710 high-resolution microarray scanner (Innopsys Life Sciences).

    other:

    Article Title: A comprehensive pharmacological survey across heterogeneous patient-derived glioblastoma stem cell models
    Article Snippet: ladder (Invitrogen, #LC5925) followed by transfer to PVDF membrane (Bio-Rad, Trans -blot Turbo pack, #1704156) and blocking with 5% BSA/TBS-Tween (0.1% (v/v)).

    Fluorescence:

    Article Title: Conserved genetic and antigenic profiles of Pf12 and Pf41 in asymptomatic Plasmodium falciparum carriers in Tanzania.
    Article Snippet: .. Bound IgG antibodies were detected using Alexa Fluor 546-conjugated goat antihuman IgG (10 ng/μL; Invitrogen), and fluorescence signals were acquired using the InnoScan 300 microarray scanner (INNOPSYS, Carbonne, France). ..

    Article Title: Sequencing-free On-chip detection of lung cancer by fluorescent enzymatic profiling of cfDNA methylation.
    Article Snippet: .. The slides were scanned using the Innoscan 1100 scanner (Innopsys), and fluorescence signal intensities were background subtracted and quantified with the Mapix software (Innopsys). ..

    Blocking Assay:

    Article Title: A comprehensive pharmacological survey across heterogeneous patient-derived glioblastoma stem cell models
    Article Snippet: Clamps were removed and microarrays were washed three times for 5 min in PBS-T. Microarrays were then blocked for 10min with Super G Blocking Buffer at room temperature and again washed three times for 5 min in PBS-T. Three millilitres (3 mL) of IRDye 800CW Streptavidin (LI-COR Biosciences) was diluted 1:5000 in PBS-T supplemented with 5% BSA, 10% SuperG Blocking Buffer. .. Following blocking buffer (Superblock T20) for 10 min the detection of marker antibodies was performed in a two-step sequential assay (1) incubation of an array with primary analyte-specific antibody in blocking buffer for 60 min at room temperature and (2) removal of excess antibody by washing arrays with PBS-T, followed by a further incubation with blocking buffer and PBS-T washes, and incubation secondary antibody (Dylight-800-labeled anti-species antibodies diluted 1:2500 in Superblock T20) for 30 min. After further washing and slide drying, the arrays were imaged in the Innopsys Innoscan 710 scanner. .. Microarrays were dried then scanned at 795 nm on the InnoScan 710 high-resolution microarray scanner (Innopsys Life Sciences).

    Marker:

    Article Title: A comprehensive pharmacological survey across heterogeneous patient-derived glioblastoma stem cell models
    Article Snippet: Clamps were removed and microarrays were washed three times for 5 min in PBS-T. Microarrays were then blocked for 10min with Super G Blocking Buffer at room temperature and again washed three times for 5 min in PBS-T. Three millilitres (3 mL) of IRDye 800CW Streptavidin (LI-COR Biosciences) was diluted 1:5000 in PBS-T supplemented with 5% BSA, 10% SuperG Blocking Buffer. .. Following blocking buffer (Superblock T20) for 10 min the detection of marker antibodies was performed in a two-step sequential assay (1) incubation of an array with primary analyte-specific antibody in blocking buffer for 60 min at room temperature and (2) removal of excess antibody by washing arrays with PBS-T, followed by a further incubation with blocking buffer and PBS-T washes, and incubation secondary antibody (Dylight-800-labeled anti-species antibodies diluted 1:2500 in Superblock T20) for 30 min. After further washing and slide drying, the arrays were imaged in the Innopsys Innoscan 710 scanner. .. Microarrays were dried then scanned at 795 nm on the InnoScan 710 high-resolution microarray scanner (Innopsys Life Sciences).

    Imaging:

    Article Title: Comparative analysis and correlation of cancer hotspot proteins and cell markers in tumor-normal adjacent breast and kidney samples using RPPA and LC-MS.
    Article Snippet: In addition, one slide per RPPA run was stained by FCF Fast Green (Sigma F7258) for total protein normalization of spots during the imaging process (see below). .. Slides were scanned as described below within a few hours after the last washing step and following a short drying protocol by spinning at 300 rpm for 5 min. (v) Slide/blot imaging and spot quantification: Slides were loaded (24 slides at a time, using an autoloader) in an Innoscan 710-IR (Innopsys Inc). ..

    Software:

    Article Title: Sequencing-free On-chip detection of lung cancer by fluorescent enzymatic profiling of cfDNA methylation.
    Article Snippet: .. The slides were scanned using the Innoscan 1100 scanner (Innopsys), and fluorescence signal intensities were background subtracted and quantified with the Mapix software (Innopsys). ..



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    Image Search Results


    Flow chart of microarray data analysis. The agilent single color microarray platform was used having more than 58,000 transcripts and further data processing was done using agilent GeneSpring software followed by pathway analysis using GSEA database. Numbers in parentheses illustrates number of pathways enriched in each category using GSEA database. DEGs-Differentially expressed genes, ANOVA-Analysis of variance, BP-Biological pathway, CC-Cellular component, MF-Molecular function.

    Journal: bioRxiv

    Article Title: Synovial fluid transcriptome dynamics in osteoarthritis progression: Implications in pathogenesis

    doi: 10.1101/2024.06.24.600143

    Figure Lengend Snippet: Flow chart of microarray data analysis. The agilent single color microarray platform was used having more than 58,000 transcripts and further data processing was done using agilent GeneSpring software followed by pathway analysis using GSEA database. Numbers in parentheses illustrates number of pathways enriched in each category using GSEA database. DEGs-Differentially expressed genes, ANOVA-Analysis of variance, BP-Biological pathway, CC-Cellular component, MF-Molecular function.

    Article Snippet: Following hybridization, the array slides were washed and scanned with an Agilent SureScan High-Resolution DNA Microarray Scanner.

    Techniques: Microarray, Software

    The 11 genes were detected in the microarray analysis and in the human dataset. A correlation between FCs of genes. B heatmap with hierarchical clustering of FCs of the 11 genes commonly detected in the murine neurospheres and AD patients’ tissues

    Journal: Cell & Bioscience

    Article Title: Bradykinin promotes immune responses in differentiated embryonic neurospheres carrying APP swe and PS1 dE9 mutations

    doi: 10.1186/s13578-024-01251-3

    Figure Lengend Snippet: The 11 genes were detected in the microarray analysis and in the human dataset. A correlation between FCs of genes. B heatmap with hierarchical clustering of FCs of the 11 genes commonly detected in the murine neurospheres and AD patients’ tissues

    Article Snippet: The arrays were then washed as described on Agilent SSPE wash protocol v. 2.1 and scanned with a High-Resolution Microarray Scanner (Agilent Technologies).

    Techniques: Microarray