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microarray imager  (LI-COR)


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    Structured Review

    LI-COR microarray imager
    Microarray Imager, supplied by LI-COR, used in various techniques. Bioz Stars score: 99/100, based on 13659 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/high+microarray+scanner/Odyssey+CLx+Imaging+System/pmc05536345-91-15-27
    Average 99 stars, based on 13659 article reviews
    microarray imager - by Bioz Stars, 2026-11
    99/100 stars

    Images

    Related Articles

    Imaging:

    Article Title: Neonatal Delivery of Exon Skipping Therapeutics Maximizes Brain Dystrophin Rescue in the Mdx52 Mouse Model of Duchenne Muscular Dystrophy
    Article Snippet: Membranes were incubated with IRDye 680CW goat anti-rabbit and IRDye 800CW goat anti-mouse secondary antibodies (LI-COR Biosciences, Germany). .. Fluorescent signals were acquired using an Odyssey CLx imaging system (LI-COR), and quantification was performed with Image Studio software (LI-COR). ..

    Article Title: HDAC Inhibitors Potentiate Doxorubicin Cytotoxicity in HepG2 Cells and Alter DNA-Repair- and p53-Associated Transcriptional Programs
    Article Snippet: After washing three times with TBST (TBS + 0.1% Tween-20), membranes were incubated with IRDye secondary antibodies (LI-COR Biosciences, Lincoln, NE, USA) diluted 1:10,000, goat anti-mouse IRDye 680RD (Cat# 926-68070) or goat anti-rabbit IRDye 800CW (Cat# 926-32211). .. Membranes were imaged using the Odyssey CLx Imaging System (LI-COR Biosciences). .. Band intensities were quantified using ImageJ software version 1.51n and normalized to β-actin, GAPDH or total histone H3 as indicated.

    Article Title: Raf-MEK-ERK signaling mediates FLT3-induced mechanical allodynia and reduced morphine analgesic efficacy in male rodents
    Article Snippet: .. Signals were detected using either an Odyssey CLx Imaging System (LI-COR Biosciences) or ECL reagents and a ChemiDoc XRS system (Bio-Rad), and quantified using Image Studio or Image Lab software, respectively. ..

    Article Title: Cytoplasmic recapping commences the translation of a micropeptide, MARS from MALAT1 after the removal of an inhibitory RNA G-quadruplex
    Article Snippet: After washing in PBS with 0.1% Tween-20 (PBS-T), the membranes were incubated in secondary antibodies DyLight 800-conjugated donkey anti-mouse-IgG (#SA535521; Invitrogen, USA), or Alexa Fluor 680-conjugated donkey anti-rabbit-IgG (#A143; Invitrogen) at 1:10,000 dilution in PBS-T]. .. The immunoblots were scanned using an Odyssey CLx infrared imaging system (Li-cor, USA), and the background-corrected intensities of bands were determined using ImageStudio (Li-cor, USA). .. Cells were grown on coverslips and were fixed with freshly prepared fixing solution containing 4% paraformaldehyde (EMS) and 0.2% Triton X-100 (Bio-Rad, USA) in 1× PBS for 10 min at room temperature.

    Article Title: Non-canonical IL-2 signaling facilitates peripheral regulatory T cell differentiation
    Article Snippet: Coprecipitated CD25 and CCR7 were detected by immunoblotting using an anti HA antibody (BioLegend, 901513) and an anti-CCR7 antibody (Novus Biologicals, NBP2-67324). .. Band intensities were quantified using an Odyssey CLx infrared imaging system (LI COR Biosciences). ..

    Article Title: Multiple Pol determinants constrain koala retrovirus A replication
    Article Snippet: Secondary antibodies included goat anti-rabbit IgG (1:10,000; LI-COR Biosciences; cat. no. 926-32211), goat anti-rat IgG (1:10,000; LI-COR Biosciences; cat. no. 925-32219), and goat anti-mouse IgG (1:10,000; LI-COR Biosciences; cat. no. 926-68070). .. Membranes were then washed three times for 5 min each with 1× TBS-T and imaged using an Odyssey CLx Imaging System (LI-COR Biosciences). ..

    Article Title: TBX3 is required for the differentiation of a subset of retinal ganglion and cone photoreceptor cells
    Article Snippet: The membrane was incubated with rabbit anti-TBX3 (Invitrogen, #42-800 1:1000 v/v) and mouse anti-β-actin (Li-Cor, #926-4212 1:1000 v/v) antibodies overnight at 4°C with gentle agitation, followed by 1-hour incubation with appropriate secondary antibodies at room temperature. .. Bands were visualized using the Odyssey CLx Infrared Imaging System (Li-Cor). .. Images were obtained in a Leica DM600B fluorescence microscope (Leica, Wetzlar, Germany) coupled to a MicroPublisher 6 camera (Teledyne Photometrics, Tucson, AZ, USA).

    Article Title: Small GTPases RhoA and RhoC coordinately regulate cardiomyocyte size in the mouse heart
    Article Snippet: For alkaline phosphatase-based detection, membranes were incubated with IgG-AP-conjugated secondary antibodies (Santa Cruz Biotechnology, Dallas TX; 1:2,500), developed using ECF substrate (GE Healthcare, Buckinghamshire, United Kingdom, #RPN5785), and imaged with a Gel Doc XR+ system using Image Lab software (Bio-Rad Laboratories, Hercules, CA; RRID:SCR_014210). .. Alternatively, membranes were incubated with IRDye 800CW- or IRDye 680RD-conjugated secondary antibodies (LI-COR Biosciences, Lincoln, NE; 1:3,000) and imaged using an Odyssey CLx Imaging System (LI-COR Biosciences). .. Band intensities were quantified using LI-COR Image Studio software (RRID:SCR_015795).

    Software:

    Article Title: Neonatal Delivery of Exon Skipping Therapeutics Maximizes Brain Dystrophin Rescue in the Mdx52 Mouse Model of Duchenne Muscular Dystrophy
    Article Snippet: Membranes were incubated with IRDye 680CW goat anti-rabbit and IRDye 800CW goat anti-mouse secondary antibodies (LI-COR Biosciences, Germany). .. Fluorescent signals were acquired using an Odyssey CLx imaging system (LI-COR), and quantification was performed with Image Studio software (LI-COR). ..

    Article Title: Raf-MEK-ERK signaling mediates FLT3-induced mechanical allodynia and reduced morphine analgesic efficacy in male rodents
    Article Snippet: .. Signals were detected using either an Odyssey CLx Imaging System (LI-COR Biosciences) or ECL reagents and a ChemiDoc XRS system (Bio-Rad), and quantified using Image Studio or Image Lab software, respectively. ..

    Western Blot:

    Article Title: Cytoplasmic recapping commences the translation of a micropeptide, MARS from MALAT1 after the removal of an inhibitory RNA G-quadruplex
    Article Snippet: After washing in PBS with 0.1% Tween-20 (PBS-T), the membranes were incubated in secondary antibodies DyLight 800-conjugated donkey anti-mouse-IgG (#SA535521; Invitrogen, USA), or Alexa Fluor 680-conjugated donkey anti-rabbit-IgG (#A143; Invitrogen) at 1:10,000 dilution in PBS-T]. .. The immunoblots were scanned using an Odyssey CLx infrared imaging system (Li-cor, USA), and the background-corrected intensities of bands were determined using ImageStudio (Li-cor, USA). .. Cells were grown on coverslips and were fixed with freshly prepared fixing solution containing 4% paraformaldehyde (EMS) and 0.2% Triton X-100 (Bio-Rad, USA) in 1× PBS for 10 min at room temperature.

    Infrared:

    Article Title: Cytoplasmic recapping commences the translation of a micropeptide, MARS from MALAT1 after the removal of an inhibitory RNA G-quadruplex
    Article Snippet: After washing in PBS with 0.1% Tween-20 (PBS-T), the membranes were incubated in secondary antibodies DyLight 800-conjugated donkey anti-mouse-IgG (#SA535521; Invitrogen, USA), or Alexa Fluor 680-conjugated donkey anti-rabbit-IgG (#A143; Invitrogen) at 1:10,000 dilution in PBS-T]. .. The immunoblots were scanned using an Odyssey CLx infrared imaging system (Li-cor, USA), and the background-corrected intensities of bands were determined using ImageStudio (Li-cor, USA). .. Cells were grown on coverslips and were fixed with freshly prepared fixing solution containing 4% paraformaldehyde (EMS) and 0.2% Triton X-100 (Bio-Rad, USA) in 1× PBS for 10 min at room temperature.

    Article Title: Non-canonical IL-2 signaling facilitates peripheral regulatory T cell differentiation
    Article Snippet: Coprecipitated CD25 and CCR7 were detected by immunoblotting using an anti HA antibody (BioLegend, 901513) and an anti-CCR7 antibody (Novus Biologicals, NBP2-67324). .. Band intensities were quantified using an Odyssey CLx infrared imaging system (LI COR Biosciences). ..

    Article Title: TBX3 is required for the differentiation of a subset of retinal ganglion and cone photoreceptor cells
    Article Snippet: The membrane was incubated with rabbit anti-TBX3 (Invitrogen, #42-800 1:1000 v/v) and mouse anti-β-actin (Li-Cor, #926-4212 1:1000 v/v) antibodies overnight at 4°C with gentle agitation, followed by 1-hour incubation with appropriate secondary antibodies at room temperature. .. Bands were visualized using the Odyssey CLx Infrared Imaging System (Li-Cor). .. Images were obtained in a Leica DM600B fluorescence microscope (Leica, Wetzlar, Germany) coupled to a MicroPublisher 6 camera (Teledyne Photometrics, Tucson, AZ, USA).

    Transcranial Magnetic Stimulation:

    Article Title: Multiple Pol determinants constrain koala retrovirus A replication
    Article Snippet: Secondary antibodies included goat anti-rabbit IgG (1:10,000; LI-COR Biosciences; cat. no. 926-32211), goat anti-rat IgG (1:10,000; LI-COR Biosciences; cat. no. 925-32219), and goat anti-mouse IgG (1:10,000; LI-COR Biosciences; cat. no. 926-68070). .. Membranes were then washed three times for 5 min each with 1× TBS-T and imaged using an Odyssey CLx Imaging System (LI-COR Biosciences). ..

    Incubation:

    Article Title: Small GTPases RhoA and RhoC coordinately regulate cardiomyocyte size in the mouse heart
    Article Snippet: For alkaline phosphatase-based detection, membranes were incubated with IgG-AP-conjugated secondary antibodies (Santa Cruz Biotechnology, Dallas TX; 1:2,500), developed using ECF substrate (GE Healthcare, Buckinghamshire, United Kingdom, #RPN5785), and imaged with a Gel Doc XR+ system using Image Lab software (Bio-Rad Laboratories, Hercules, CA; RRID:SCR_014210). .. Alternatively, membranes were incubated with IRDye 800CW- or IRDye 680RD-conjugated secondary antibodies (LI-COR Biosciences, Lincoln, NE; 1:3,000) and imaged using an Odyssey CLx Imaging System (LI-COR Biosciences). .. Band intensities were quantified using LI-COR Image Studio software (RRID:SCR_015795).



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    Image Search Results


    Flow chart of microarray data analysis. The agilent single color microarray platform was used having more than 58,000 transcripts and further data processing was done using agilent GeneSpring software followed by pathway analysis using GSEA database. Numbers in parentheses illustrates number of pathways enriched in each category using GSEA database. DEGs-Differentially expressed genes, ANOVA-Analysis of variance, BP-Biological pathway, CC-Cellular component, MF-Molecular function.

    Journal: bioRxiv

    Article Title: Synovial fluid transcriptome dynamics in osteoarthritis progression: Implications in pathogenesis

    doi: 10.1101/2024.06.24.600143

    Figure Lengend Snippet: Flow chart of microarray data analysis. The agilent single color microarray platform was used having more than 58,000 transcripts and further data processing was done using agilent GeneSpring software followed by pathway analysis using GSEA database. Numbers in parentheses illustrates number of pathways enriched in each category using GSEA database. DEGs-Differentially expressed genes, ANOVA-Analysis of variance, BP-Biological pathway, CC-Cellular component, MF-Molecular function.

    Article Snippet: Following hybridization, the array slides were washed and scanned with an Agilent SureScan High-Resolution DNA Microarray Scanner.

    Techniques: Microarray, Software

    The 11 genes were detected in the microarray analysis and in the human dataset. A correlation between FCs of genes. B heatmap with hierarchical clustering of FCs of the 11 genes commonly detected in the murine neurospheres and AD patients’ tissues

    Journal: Cell & Bioscience

    Article Title: Bradykinin promotes immune responses in differentiated embryonic neurospheres carrying APP swe and PS1 dE9 mutations

    doi: 10.1186/s13578-024-01251-3

    Figure Lengend Snippet: The 11 genes were detected in the microarray analysis and in the human dataset. A correlation between FCs of genes. B heatmap with hierarchical clustering of FCs of the 11 genes commonly detected in the murine neurospheres and AD patients’ tissues

    Article Snippet: The arrays were then washed as described on Agilent SSPE wash protocol v. 2.1 and scanned with a High-Resolution Microarray Scanner (Agilent Technologies).

    Techniques: Microarray