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hfob1 19 complete medium  (Elabscience Biotechnology)


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    Elabscience Biotechnology hfob1 19 complete medium
    Hfob1 19 Complete Medium, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hfob1+19/hFOB+1%2E19+Cell+Complete+Medium/pm36769824-53-33-38
    Average 91 stars, based on 4 article reviews
    hfob1 19 complete medium - by Bioz Stars, 2026-10
    91/100 stars

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    Related Articles

    Cell Culture:

    Article Title: miR-197-3p Promotes Osteosarcoma Stemness and Chemoresistance by Inhibiting SPOPL.
    Article Snippet: The human osteoblast hFOB1.19 (catalog: CRL-11372), human osteosarcoma cell lines U2OS (catalog: HTB-96), and MG63 (catalog: CRL-1427) were sourced from the American Type Culture Collection (Manassas, VA, USA). .. U2OS and MG63 were cultured in Dulbecco’s modified Eagle medium (catalog: BE12-604F, Lonza, Basel, Switzerland) supplemented with 10% fetal bovine serum (catalog: F7524, Sigma-Aldrich, St. Louis, MO, USA). hFOB1.19 cells were cultivated in hFOB1.19 complete medium (catalog: EP-ML-0353, Elabscience, Wuhan, China). ..

    Article Title: miR-197-3p Promotes Osteosarcoma Stemness and Chemoresistance by Inhibiting SPOPL
    Article Snippet: The human osteoblast hFOB1.19 (catalog: CRL-11372), human osteosarcoma cell lines U2OS (catalog: HTB-96), and MG63 (catalog: CRL-1427) were sourced from the American Type Culture Collection (Manassas, VA, USA). .. U2OS and MG63 were cultured in Dulbecco’s modified Eagle medium (catalog: BE12-604F, Lonza, Basel, Switzerland) supplemented with 10% fetal bovine serum (catalog: F7524, Sigma-Aldrich, St. Louis, MO, USA). hFOB1.19 cells were cultivated in hFOB1.19 complete medium (catalog: EP-ML-0353, Elabscience, Wuhan, China). ..

    Modification:

    Article Title: miR-197-3p Promotes Osteosarcoma Stemness and Chemoresistance by Inhibiting SPOPL.
    Article Snippet: The human osteoblast hFOB1.19 (catalog: CRL-11372), human osteosarcoma cell lines U2OS (catalog: HTB-96), and MG63 (catalog: CRL-1427) were sourced from the American Type Culture Collection (Manassas, VA, USA). .. U2OS and MG63 were cultured in Dulbecco’s modified Eagle medium (catalog: BE12-604F, Lonza, Basel, Switzerland) supplemented with 10% fetal bovine serum (catalog: F7524, Sigma-Aldrich, St. Louis, MO, USA). hFOB1.19 cells were cultivated in hFOB1.19 complete medium (catalog: EP-ML-0353, Elabscience, Wuhan, China). ..

    Article Title: miR-197-3p Promotes Osteosarcoma Stemness and Chemoresistance by Inhibiting SPOPL
    Article Snippet: The human osteoblast hFOB1.19 (catalog: CRL-11372), human osteosarcoma cell lines U2OS (catalog: HTB-96), and MG63 (catalog: CRL-1427) were sourced from the American Type Culture Collection (Manassas, VA, USA). .. U2OS and MG63 were cultured in Dulbecco’s modified Eagle medium (catalog: BE12-604F, Lonza, Basel, Switzerland) supplemented with 10% fetal bovine serum (catalog: F7524, Sigma-Aldrich, St. Louis, MO, USA). hFOB1.19 cells were cultivated in hFOB1.19 complete medium (catalog: EP-ML-0353, Elabscience, Wuhan, China). ..



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    a. All variant–gene pairs within a receptive field of 256kbp are scored by Rosalind. Genes above the elbow threshold are kept as candidate causal genes. b. The distribution of protein-coding genes around the 1103 lead variants from the eBMD GWAS (Morris et al. ) when using a window of 256kbp. c. Overlap of Rosalind-predicted genes with nearest genes is shown, with genes known to regulate eBMD highlighted. d. Mineralization assay experimental workflow: arrayed CRISPR/Cas9 knock-out screen <t>of</t> <t>hFOB1.19</t> human osteoblasts. e. Plot shows SNP RSID-gene pairs, where at least one hit was observed in the osteoblast mineralisation assay. Hit calling was performed using Region of Practical Equivalence (ROPE) analysis and results show hits at ROPE 0.5 or greater (‘moderate’ effect size) f. Experimental validation summary, with assay “hits” represented in blue and “no effect” represented in red.
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    Image Search Results


    The role of miR-27a-3p in osteosarcoma cells. (A) Expression levels of miR-27a-3p in normal osteoblasts hFOB1.19 and osteosarcoma cells Saos-2 and MG-63. (B,C) The influence of introducing miR-27a-3p mimic and inhibitor on the expression of miR-27a-3p in osteosarcoma cell lines MG-63 and SAOS-2. (D-F) CCK-8 assay, Transwell migration assay and FCM assay were used to detect the changes in cell proliferation, migration ability and apoptosis levels of osteosarcoma cells MG-63 and Saos-2 transfected with miR-27a-3p mimic and inhibitor. Stained with crystal violet; magnification: ×200. *, P<0.05; **, P<0.01. CCK-8, Cell Counting Kit-8; FCM, flow cytometry; NC, negative control.

    Journal: Translational Cancer Research

    Article Title: Molecular mechanism of miR-27a-3p targeting FBXW7 regulating the malignant behavior of osteosarcoma cells

    doi: 10.21037/tcr-2025-aw-2290

    Figure Lengend Snippet: The role of miR-27a-3p in osteosarcoma cells. (A) Expression levels of miR-27a-3p in normal osteoblasts hFOB1.19 and osteosarcoma cells Saos-2 and MG-63. (B,C) The influence of introducing miR-27a-3p mimic and inhibitor on the expression of miR-27a-3p in osteosarcoma cell lines MG-63 and SAOS-2. (D-F) CCK-8 assay, Transwell migration assay and FCM assay were used to detect the changes in cell proliferation, migration ability and apoptosis levels of osteosarcoma cells MG-63 and Saos-2 transfected with miR-27a-3p mimic and inhibitor. Stained with crystal violet; magnification: ×200. *, P<0.05; **, P<0.01. CCK-8, Cell Counting Kit-8; FCM, flow cytometry; NC, negative control.

    Article Snippet: Osteosarcoma cell lines MG-63 (RRID: CVCL_0426) and Saos-2 (RRID: CVCL_0548), as well as the normal human osteoblast hFOB1.19 (RRID: CVCL_3889), were sourced from Servicebio company (Wuhan, China).

    Techniques: Expressing, CCK-8 Assay, Transwell Migration Assay, Migration, Transfection, Staining, Cell Counting, Flow Cytometry, Negative Control

    The expression of FBXW7 in osteosarcoma cells. (A) The mRNA levels of FBXW7 in normal osteoblasts hFOB1.19 and osteosarcoma cells Saos-2, MG-63 were also analyzed. (B,C) To explore the impact of FBXW7 on the biological behavior of osteosarcoma cells Saos-2 and MG-63, the researchers performed transfections on these two osteosarcoma cell lines using pcDNA3.1-FLAG-FBXW7 and siRNA-FBXW7. (D,E) Effect of transfection of pcDNA3.1-FLAG-FBXW7 and siRNA-FBXW7 on expression of FBXW7 and β-catenin in osteosarcoma cells MG-63 (D) and Saos-2 (E). *, P<0.05; **, P<0.01; ***, P<0.001; ****, P<0.0001; ns, not significant. NC, negative control; OE, overexpression.

    Journal: Translational Cancer Research

    Article Title: Molecular mechanism of miR-27a-3p targeting FBXW7 regulating the malignant behavior of osteosarcoma cells

    doi: 10.21037/tcr-2025-aw-2290

    Figure Lengend Snippet: The expression of FBXW7 in osteosarcoma cells. (A) The mRNA levels of FBXW7 in normal osteoblasts hFOB1.19 and osteosarcoma cells Saos-2, MG-63 were also analyzed. (B,C) To explore the impact of FBXW7 on the biological behavior of osteosarcoma cells Saos-2 and MG-63, the researchers performed transfections on these two osteosarcoma cell lines using pcDNA3.1-FLAG-FBXW7 and siRNA-FBXW7. (D,E) Effect of transfection of pcDNA3.1-FLAG-FBXW7 and siRNA-FBXW7 on expression of FBXW7 and β-catenin in osteosarcoma cells MG-63 (D) and Saos-2 (E). *, P<0.05; **, P<0.01; ***, P<0.001; ****, P<0.0001; ns, not significant. NC, negative control; OE, overexpression.

    Article Snippet: Osteosarcoma cell lines MG-63 (RRID: CVCL_0426) and Saos-2 (RRID: CVCL_0548), as well as the normal human osteoblast hFOB1.19 (RRID: CVCL_3889), were sourced from Servicebio company (Wuhan, China).

    Techniques: Expressing, Transfection, Negative Control, Over Expression

    a. All variant–gene pairs within a receptive field of 256kbp are scored by Rosalind. Genes above the elbow threshold are kept as candidate causal genes. b. The distribution of protein-coding genes around the 1103 lead variants from the eBMD GWAS (Morris et al. ) when using a window of 256kbp. c. Overlap of Rosalind-predicted genes with nearest genes is shown, with genes known to regulate eBMD highlighted. d. Mineralization assay experimental workflow: arrayed CRISPR/Cas9 knock-out screen of hFOB1.19 human osteoblasts. e. Plot shows SNP RSID-gene pairs, where at least one hit was observed in the osteoblast mineralisation assay. Hit calling was performed using Region of Practical Equivalence (ROPE) analysis and results show hits at ROPE 0.5 or greater (‘moderate’ effect size) f. Experimental validation summary, with assay “hits” represented in blue and “no effect” represented in red.

    Journal: bioRxiv

    Article Title: A DNA foundation model predicts osteoporosis risk genes without proximity bias

    doi: 10.64898/2026.03.09.707383

    Figure Lengend Snippet: a. All variant–gene pairs within a receptive field of 256kbp are scored by Rosalind. Genes above the elbow threshold are kept as candidate causal genes. b. The distribution of protein-coding genes around the 1103 lead variants from the eBMD GWAS (Morris et al. ) when using a window of 256kbp. c. Overlap of Rosalind-predicted genes with nearest genes is shown, with genes known to regulate eBMD highlighted. d. Mineralization assay experimental workflow: arrayed CRISPR/Cas9 knock-out screen of hFOB1.19 human osteoblasts. e. Plot shows SNP RSID-gene pairs, where at least one hit was observed in the osteoblast mineralisation assay. Hit calling was performed using Region of Practical Equivalence (ROPE) analysis and results show hits at ROPE 0.5 or greater (‘moderate’ effect size) f. Experimental validation summary, with assay “hits” represented in blue and “no effect” represented in red.

    Article Snippet: hFOB1.19 (ATCC) were cultured in flasks in DMEM/F-12, 10% FBS (Sigma F7524 lot.0001663052), antibiotic/antimycotic (Gibco 15240-062) and G418 sulfate (ab144261) at 34oC until 80-90% confluent.

    Techniques: Variant Assay, Mineralization Assay, CRISPR, Knock-Out, Biomarker Discovery